Fluorescence : One phenomenon, many

applications Jussi Kinnunen, MSc (Physics), PhD student

Oral presentation

CREATE Gjøvik

There will be:

• Short introduction to fluorescence• Few applications

– Something about bioanalytics– Coloring or whitening merchandise – Arts

• Measurements and data analysis – by using two monochromator –method

Fluorescence : One phenomenon, many applications / Jussi Kinnunen

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What is fluorescence?

For me it is fancy colours

Fluorescence : One phenomenon, many applications / Jussi Kinnunen

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Light interaction with matter

When light comes• Absorption

– Time about 10-15 s

• Scattering– Time about 0 s

When light goes• Fluorescence emission

– Lifetime about 10-8(10-10) s

– Lower energy photons than the one(s) absorbed

• Phosphorescence– Lifetime >10-8 s– Lower energy photons

than the one(s) absorbed

• Scattering– Energy if the photon is

close to the incident one(s)

Fluorescence : One phenomenon, many applications / Jussi Kinnunen

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A FP

Applications: Bioanalytics

Fast detection of small quantities of anything• Idea:

– Choose a receptor for the target

– Attach label (reporter) to the receptor

– Illuminate and see • Where is your target

– e.g. cancer cells• Was there any of

your target – e.g. DNA

sequencingFluorescence : One phenomenon, many applications / Jussi Kinnunen

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Applications: Industry

Making things white

Fluorescence : One phenomenon, many applications / Jussi Kinnunen

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Applications: Art

Masterpiece (Miera, 1950’s), white light and UVPhoto courtesy of Nick Padalino

Fluorescence : One phenomenon, many applications / Jussi Kinnunen

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Color measurements of fluorescence

Two monochromator method

Fluorescence : One phenomenon, many applications / Jussi Kinnunen

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Measured data

• Donaldson matrix is calculated from the measured data

•The reflectance – On diagonal

•The fluorescence– Off diagonal

Fluorescence : One phenomenon, many applications / Jussi Kinnunen

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Simulations

Simulation with the scientific brown

Fluorescence : One phenomenon, many applications / Jussi Kinnunen

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Simulation with the scientific brown

Fluorescence : One phenomenon, many applications / Jussi Kinnunen

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Lights

• Simulations under – Tungsten light (green)– Daylight simulator

(blue)– White led (red)– Fluorescent light

(cyan)

Fluorescence : One phenomenon, many applications / Jussi Kinnunen

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Under “smooth” light sources

Halogen light Daylight simulator

Fluorescence : One phenomenon, many applications / Jussi Kinnunen

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reflectance

fluorescence

total

The black curve is measured under

polycromatic light

Under “peaky” light sources

Led light Fluorescent light

Fluorescence : One phenomenon, many applications / Jussi Kinnunen

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reflectance

fluorescence

total

The black curve is measured under

polycromatic light

Thank you.

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