Gioi han phat phepthir D6i thfr coj/Phamvi

D6i tuongphep thfrTT Ten phep thir

127

128

129 Xet nghiem tetracycline bangphirong phap ELISA

Thirc anchan nuoi

0,3 ppb

Gioi han phathien (neu Phuong phap thu'

coj/Pham vido

Tieu chuan C(J sa,

Maso:TYV6-HDPP.HL-02Tieu chuan co so

10 ppbMas6:TYV6-HDPP.HL-07Tieu chuan co so

10 ppb Mas6:TYV6-HDPP.HL-08

Xet nghiem Furazolidone (AOZ)130 bang phuong phap ELISA

Thuc anchan nuoi

Xet nghiem Furaltadone (AMOZ)131 bang phirong phap ELISA

Thuc anchan nuoi

Tieu chuan C(J so1 ppb

Mas6:TYV 6-HDPP .HL-04Tieu chuan C(J so

1 ppbMas6:TYV6-HDPP.HL-03

19

Gioi han phat

TT Ten phep thu'D8i tuong hien (neu Phuong phap thirphep thif co)/Ph?ID vi

do

Xac dinh ADN ciru bang ky thuat Nguyen li~u thirc 0,5%Tieu chuan CO" 56":

116 Realtime PCR an chan nuoi va Ma so:thirc an chan nuoi TYV6-HDPP.SH-02

Xac dinh ADN heo bang ky thuat Nguyen lieu thirc 0,5%Tieu chuan CO" so:

117 Realtime PCR an chan nuoi va Mas6:thirc an chan nuoi TYV6-HDPP.SH-03

Xac dinh ADN ga bang ky thuatNguyen lieu thirc Tieu chuan CO" so':an chan nuoi va 0,5%

118 Realtime PCR thirc an chan nuoi Mas6:TYV6-HDPP.SH-04

Xac dinh ADN de bang ky thuat Nguyen lieu thirc 0,5%Tieu chuan CO" SCJ"

119 Realtime PCR an chan nuoi va Mast>:thirc an chan nuoi TYV6-HDPP.SH-OS

Xac dinh ADN ngua bang ky Nguyen lieu thirc 0,5%Tieu chuan CO" so':

120 thuat Realtime PCR an chan nuoi va Mas6:thirc an chan nuoi TYV6-HDPP.SH-06

b) Phep thir Hoa hoc

Xet nghiem dinh hrong Aflatoxin Tieu chuan CO" so'

121 G2, G1,B2, B1 tren thuc an chanThirc an 2ppb

nuoi b~g ky thu~t HPLC-FLDchan nuoi Mas6:

TYV6-HDPP.HL-19

Tieu chuan CO" sa

122 Xet nghi~m Clenbuterol Thuc an 40 ppb Mas6:

b~g ky thu~t ELISA chan nuoiTYV6-HDPP.HL-27

-Tieu chuAn CO" s

123 Xet nghi~m Salbutamol Thuc an 40 ppbb~g ky thu~t ELISA chan nuoi Mas6:

TYV6-HDPP.HL-33Tieu chuAn co sa

124 Xet nghi~m Ractopamin Thuc an 10 ppbb~ng ky thu~t ELISA chan nuoi Mas6:

TYV6-HDPP.HL-34

Xac dinh ham luqng Nita va tinh Thuc an TCVN 4328:2001

125 ham luqng protein tho trong mftu chan nuoi Mas6:thuc an chan nuoi TYV6-HDPP.HL-16

Xac dinh de>fun va ham luqng TCVN 4326:2001

126 ch~t bay hoi khac trong thuc an Thuc an Mas6:chan nuoi chan nuoi TYV6-HDPP.HL-17

18

, .

TT TeD phep thir a6i nrongphep thir

~

(~. ~~~~.~~n chan nuoi

t(f,l/l *,,~~~--+-------------_;~l~~!Z~~r~~~~~i~fi!~~'~_~~.~-------1----------~T~C=VN~~68~4~6~:2~0~07--~

Phuong phap dinh,lu ~e/ n lieu thirc (ISO 7251:2005)109 Escherichia coli ban tL *. ~.... an nuoi va 3 MPN/g Ma s6:

MPN ~.-~ thirc an chan nuoi 0,3 MPN/ml TYV6-HDPP.VT-03.01

Gioi han phathien (neu

co)/Ph<;tm vido

Phuong phap thif

TYV6-HDPP.VT-07.01

110Phuong phap dinh lUQTIgColiforms bang ky thuat MPN

Nguyen lieu thircan chan nuoi vathirc an chan nuoi

3 MPN/g0,3 MPN/ml

TCVN 4882:2007(ISO 4831: 2006)

Mas6:TYV6-HDPP.VT-02.01

111

Phuong phap dinh hrongEscherichia coli cho phan imgdirong tinh voi p- glucuronidase

Phuong phap phat hien112 Salmonella spp tren rna thach



10 CFU/g1CFU/ml

TCVN 7924-2:2008(ISO16649-2:2001)

Mas6:TYV6-HDPP.VT-03.02TCVN 4829:2005Modify

10 CFU/25 g(ml) (ISO 6579 :2002)

Mas6:TYV6-HDPP.YT-07.03

Phat hien Vibrio spp - C6 khanang gay benh duong ruot - Phan

113 1: phat hien Vibrioparahaemolyticus va Vibriocholera


10 CFUI 25 g(ml)

TCVN 7905-1:2008(ISOITS 21872-1:2007)


Xac dinh ADN bo va ciru bAng ky114 thu~t PCR

Nguyen li~u thucan chan nuoi vathuc an chan nuoi

0,5%

TCCSSTTNSV01:2007

Mas6:TYV6-HDPP.SH-09.01

Xac dinh ADN b6 bAng ky thu~t115 Realtime PCR

Nguyen li~u thucan chan nuoi vathuc an chan nuoi

0,5%Tieu chuAn CO' sa:

Mas6:TYV6-HDPP.SH-Ol

17

D6i nrongGioi han phat

TT TeD phep thu'hien (neu Phuong phap thtl

phep thu' coj/Pham vido

Xac dinh ham hrong AOAC 980.23-2000

98 Hydroxymethylfurfural (HMF) M~t ongTCVN 5270:2008

trong mat ongMas6:TYV6-HDPP.HL-13

Xet nghiern do d9 pH tren m~u Thit va san phamTCVN 4835:2002

99 thit -2 - 19IS02917:1999

thit Mas6:TYV6-HDPP.HL-14

100 Dinh tinh H2S tren mau thit Thit va san pham TCVN 3699:1990

thit Mas6:TYV6-HDPP.HL-15

Xac dinh ham hrong nita aminiac Thit va san pham TCVN 3706:1101 trong thuc pham thit Mas6:

TYV6-HDPP.HL-Ol

Xet nghiem chloramphenicol bang Thit, cs, Tom, mat Tieu chuan co sa102 phuong phap ELISA

0,3 ppbong Mas6:

TYV6-HDPP.HL-02

Xet nghiem streptomycine bang Thit, cs, Tom, mat Tieu chuan CO" sc

103 phuong phap ELISA ong10 ppb

Mas6:TYV6-HDPP.HL-07Tieu chuan CO" sa

104 Xet nghiem tetracycline bangThit, Ca, Tom, mat 10 ppbong Mas6:

phuong phap ELISATYV6-HDPP.HL-08

Xet nghiem Furazolidone (AOZ) Thit, cs, Tom, mat Tieu chuan co sa105 bang phuong phap ELISA

1 ppbong Mas6:

TYV6-HDPP.HL-04

Xet nghi~m Furaltadone (AMOZ) Thit, cs, Tom, m~t Tieu chuan CO" sa106 bfuIg phuong phap ELISA ong 1 ppb

Mas6:TYV6-HDPP.HL-03

III. Linh V1!C thrrc an chan nuBi

a) Phep thil Sinh hQc

Nguyen li~u thucTieu chuan CO" sa

107Phat hi~n vi khu§n Salmonella an chan nuoi va

10 CFU/25 gMas6:

spp. bfuIg ky thu~t real time PCR (ml)thuc an chan nuoi TYV6-HDPP.VT-

07.05

Phuong phap phat hi~n Nguyen li~u thuc 10 CFU/ 25 g TCVN 4829:2005

108(ISO 6579:2001 Amd

Salmonella spp. tren rna th~ch an chan nuoi va (ml) 1: 2007Mas6:

16 cf

TT

87


co)/Ph:;un vido

T" h' thf Dfli nrongen p ep U' h' t·h ?_ P ep U'/'-;:VH, ~

2ppb

88 Xet nghiem Tylosinbang ky thuat ELISA Thit,

Gan, Th~20ppb

Phuong phap thfr

TYV6-HDPP.HL-33

Tieu chuan co saMas6:TYV6-HDPP.HL-29

89M~t ong 10 ppb

Mas6:~-------------+----------~TYV6-HDPP.HL-30

Xet nghiem Sulfamethazinebang ky thuat ELISA

Thit,Gan, Th~

10 ppb

Tieu chuan cc sa

90 Xet nghiem Sulfadiazinebang ky thuat ELISA

Mat ong 5 ppbTieu chuan co saMas6:TYV6-HDPP.HL-28

91 Thit, ca, tom, matong, sua ong chua

Dinh hrong Chloramphenicolbang ky thuat LC/MS/MS

0,1 ppbTieu chuan co saMas6:TYV6-HDPP.HL-26

92 Dinh luong Carbendazim bang ky M~t ongthuat LCIMS/MS

1 ppb

Tieu chuan co saMas6:TYV6-HDPP.HL-36

93 Xet nghiern Enrofloxacinebang ky thuat ELISA

Tom, Cli va thit 20 ppbTieu chuan co' saMas6:TYV6-HDPP.HL-23

Xac dinh ham hrong nuoc trong94 mat ong Mat ong 12-30%

AOAC 969.38B-2000


95 Xac dinh ham hrong dirong khir M~t ongtv do trong mat ong

TCVN 5266:1990


96

97

Xac dinh ham lUQ1lg dUOng M~t ongsaccharose trong m~t ong

Xac dinh chi s6 Diastase trong M~t ongm~t ong

TCVN 5296:1990

Mas6:TYV6-HDPP.HL-11AOAC 958.09-2000TCVN 5268:2008Mas6:TYV6-HDPP.HL-12

15 rf

Gioi han phatD6i nrong hien (neu Phirong phap thirTT Ten phep thu' phep thfr coj/Pham vi

doThit dong v~t va Tieu chu~n CO" so'Xac dinh ADN bo bang ky thuat ,san pham co nguon 0,5% Mas5:77 Realtime PCR g6c til dong v~t TYV6-HDPP.SH-Ol

Thit d9ng v~t va

Xac dinh ADN ciru bang ky thuat san pham co nguon Tieu chuan co so'g6c til dong v~t 0,5% Mas5:78 Realtime PCR

TYV6-HDPP.SH-02

Xac dinh ADN heo bang ky thuatThit d9ng v~t va Tieu chuan CO" sa,san pham co nguon 0,5% Mas5:79 Realtime PCR g6c til d9ng v~t TYV6-HDPP.SH-03

Thit dOng v~t va , Tiel! chuan co' saXac dinh ADN ga bang ky thuat san pham co nguon0,5% Mas5:80 Realtime PCR g6c til dong v~t

TYV6-HDPP.SH-04

Xac dinh ADN de bang ky thuatThit dOng v~t va , Tieu chuan co so'san pham co nguon 0,5% Mil s5:81 Realtime PCR g6c til d9ng v~t TYV6-HDPP.SH-OS

Xac dinh ADN ngva bing ky Thit d9ng v~t va Tiel! chu~n co sasan phfun co nguon 0,5% Mas5:82 thu~t Realtime PCR g6c illd9ng v~t TYV6-HDPP.SH-06

b) Phep thir Hoa h9c

Tieu chuAnco saDinh lUQ'ngTetracycline, .

Thit, Tom, Ca 60 ppbMas5:83 Oxytetracycline, ChlortetracyclmeTYV6-HDPP.HL-18bing kY thu~t HPLCTiel! chuan CO'sa0-150

Xac dinh d9 mim cua m~t ong M~t ong mm Mas5:84pfund TYV6-HDPP.HL-20

Thit, Gan, 0,2 ppb Tieu chuAnco saXet nghi~m Clenbuterol Th~

Mas5:85 bing ky thu~t ELISA2 ppb TYV6-HDPP.HL-27Nu6c tieu

5 ppbTieu chuAnCO" so'

Xet nghi~m Salbutamol Thit,86 b~ng ky thu~t ELISA Gan, Th~ Ma s5:

14

72

TT T" h' th? Dai tU'O'Dgen p ep U' h' tOh?P ep U'~PH~

Gi6i han phathien (neu

co)/Phl;llll vido

Phuong phap thu'

!,~y~~~~~eae san~&(r*l~uthit, thuyf)i~ ~m;mgStaph~loeq .~f'~~ ~~~n ~hfun ,phan ung dirong tmh vc ~~ '. ' tnrng va~. ~-7::"-y ,Coagulase (Staphylococc ;rP(j' ~lik:' pham nraureus va cac loai khac) bclng~J~: :=i1ti"ng,sua tuoithuat MPN nguyen lieu, m~t

ong va cac sanpham illmat ong

73Dinh hrong Bacillus cereus bangky thuat d~m khuan lac a 30°C

74Dinh hrong Clostridiumperfringens bang ky thuat d~mkhuan lac

75

Phat hi~n Vibrio spp - Co khanang gay b~nh duemg fUot - Phk1: phat hi~n Vibrioparahaemolyticus va Vibriocholera

76Xac dinh ADN be va cUu bAng kythu~t PCR

Tnrng va cac sanpham tir tnrng, suatuoi nguyen lieu

TNt va cac sanpham tir thit, thuysan va san phamthuy san, tnrng vacac san pham illtnrng, sua tuoinguyen lieu, matong va cae sanphAro illm~t ong

Thit va cae sanphfun ill thit, thuysan va san phAmthuy san, tning vacae san phfun tutning, sua tumnguyen li~u, m~tong va cae sanphAm tu m~t ong

Thit dong v~t vasan phAro co ngu6ng6e tll' dong v~t

TCVN 7905-1 :2008(ISO/TS 21872-1

10 CFU/25 g :2007)

(ml)

3 MPN/g0,3 MPN/ml

10 CFU/g1 CFU/ml

10 CFU/g1 CFU/ml

0,5%

TCVN 4830-3 :2005(ISO 6888-3:2004)Mas6:TYV6-HDPP.VT-04.02

TCVN 4992 : 2005(ISO 7932 :2004)Mas6:TYV6-HDPP.VT-06.01

TCVN 4991 : 2005(ISO 7937 :2004)Mas6:TYV6-HDPP.VT-05.01


TCCS STTNSV01 :2007

Mas6:TYV6-HDPP.SH-09.01

13

Gioi han phat

TT Ten phep thirD8i tuong hien (neu Phuong phap thirphep thif coj/Pham vi

doThit va cac sanpham til thit, thuysan va san pham TCVN 4882:2007

Phuong phap dinh hrongthuy san, tnrng va (ISO 4831: 2006)

68 Coliforms bang ky thuat MPNcac san pham til 3 MPN/gtnrng, sua tuoi 0,3 MPN/ml Mas6:

nguyen lieu, matTYV6-HDPP.VT-02.01

ong va cac sanpham nr mat ong

Thit va cac sanpham tir thit, thuysan va san pham TCVN 7924-2:2008

Phuong phap dinh hrong thuy san, tnrng va (ISO16649-2:2001)

69 Escherichia coli cho phan irng cac san pham nr 10 CFU/gdirong tinh voi p- glucuronidase tnrng, sua tuoi 1 CFU/ml Mas6:

nguyen lieu, m~tTYV6-HDPP.VT-03.02

ong va cac sanpham tu mat ong

Thit va cac sanpham tu thit, thuysan va san pham TCYN 4829:2005

Phuong phap phat hienthuy san, tnrng va 10 CFU/ 25 g Modify

70 Salmonella spp tren rna thachcac san pham tu (ml)

(ISO 6579 :2002)

trung, sua tuai Mas6:TYY6-HDPP.Y

nguyen li~u, m~t 07.03ong va cac sanphb tu m~t ong

Thit va cac sanphb tu thit, thuysan va san phb TCVN 7700-1:2007

Phuang phap phat hi~n va dinh thuy san, trl.'mgva (ISO 11290-1 :1996,

luong Listeria monocytogenes - cac san ph~m tu10 CFUI 25 g Amd. 1 : 2004)

71 (ml)Ph§.n 1: Phuang phap phat hi~n trung, sua tuai Mas6:

nguyen li~u, m~t TYV6-HDPP.VT-ong va cac san 09.01

phb tUm~t ong

12

TT Ten phep thfr -Dc3i nrongphep thu


co)/Ph9Jl1vido

Phuong phap tbfr

Petrifilm

64

'-=-Dinh hrong Staphylococci c6phan irng duong tinh voiCoagulase (Staphylococcusaureus va cac loai khac) bang kythuat d~m khuan lac sir dung diaPetrifilm

65

Dinh luong Staphylococci c6phan irng dirong tinh voiCoagulase (Staphylococcusaureus va cac loai khac) tren diathach bang ky thuat sir dung m6itnrong thach Baird Parker

66Phuong phap dinh luqngEscherichia coli bfutg ky thu~tMPN

67

Phuong phap dinh luqng vi sinhv~t tren ma th<;lch- Ky thu~t d~mkhu§n l<;lca 30DC

Thit va cac sanpham ill thit, thuysan va san phamthuy san

Thit va cac sanpham ill thit, thuysan va san phamthuy san, tnrng vacac san pham illtnrng, sua nroinguyen lieu, matong va cac sanpharn illmat ong

Thit va cac sanpham nr thit, thuysan va san phamthuy san, tnrng vacac san phk tiltrUng, sua tuO'inguyen li~u, m~tong va cac sanph~m til m~t ong

Thit va cac sanphk ill thit, thuysan va san phkthuy san, trung vacac san phk illtrung, sua tuO'inguyen li~u, m~tong va cae sanphk illm~t ong

10 CFU/g1 CFU/ml

10 CFU/g1 CFU/ml

3 MPN/g0,3 MPN/ml

10 CFU/g1 CFU/ml

AOAC 2003.11


TCVN 4830-1 :2005(ISO 6888-1: 1999,Amd.l :2003)Mas6:TYV6-HDPP.VT-04.01

TCVN 6846:2007(ISO 7251 :2005)Mas6:TYV6-HDPP.VT-03.01

TCVN 4884:2005(ISO 4833: 2003)


ucf

Gioi han phat

TT Ten phep thfrDBi nrong hien (neu Phuong phap thfrphep thii' coj/Pham vi

do

Tnrng va cac san TCVN 7852:2008Dinh hrong t6ng s6 nfun men va pham tit tnrng, sua

10 CFU/g (AOAC 997.02)58 nftm moe bang phirong phap su nroi nguyen lieu, Mas6:

dung rna d~m Petrifilm mat ong va cac san1 CFU/ml TYV6-HDPP.VT-

pham tit mat ong 12.01

Phat hien vi khuanTCVN 7850 : 2008

Tnrng va cac san 10 CFU/25 g (ISO/IS 22964: 2006)59 Enterobacteriaceae sakazakii pham tit tnrng, sua (ml) Mas6:

tuoi nguyen lieu TYV6-HDPP.VT-13.01

Thit va cac sanpham tit thit, thuysan va san pham Tieu chuan CO" sa

Phat hien vi khuan Salmonellathuy san, tnrng va 10 CFU/25 g

60 spp. bang ky thuat real time PCRcac san pham tit (ml) Mas6:tnrng, sua nroi TYV6-HDPP.VT-

nguyen lieu, m~t07.05

ong va cac sanpharn tit mat ong

Thit va cac sanpham ill thit, thuysan va san pham TCVN 4829:2005thuy san, tnrng va 10 CFU/25 g

(ISO 6579:2001 Amd

61 Phuong phap phat hien cac san pham ill 1: 2007Salmonella spp. tren ilia th~ch (ml) Mas6:

trlrng, sua tuai TYV6-HDPP.Vnguyen li~u, m~t 07.01ong va cae sanphfun illm~t ong

Dinh lUQ'llgStaphylococci co AOAC 2003.07phan ung duang tinh v6i

62 Coagulase (Staphylococcus Tr(rng va cac san 10 CFU/g Mas6:aureus va cac loai khac) b~ng ky phfun tit trung 1 CFU/ml TYV6-HDPP.VT-thu~t d~m khu<1nl~c su dl,ll1grna 04.03Petrifilm

Dinh lUQ'llgStaphylococci co AOAC 2003.08phan (rng duang tinh v6i Sfratuainguyen 10 CFU/g

63 Coagulase (Staphylococcus li~u 1 CFU/mlMas6:

aureus va cac loai khac) b~ng ky IYV6-HDPP.VT-

thu~t d~m khu<1nl~c su dl,ll1grna04.04

10 r;I

TT

53

54

Ten phep thir

Ph ' hie kha h;:" ~.vR ._._.\~'-JL/at ien ang t e vl-ru~~: .~chirng r6i loan h6 h~p va sinh sana 1911(PRRS) bang ky thuat LQ11ELISA

Phat hien khang th~ cum gia dmbang phan irng ngan tro ngung k~t

55 hong du trong huyet thanh (HIcum)

56

II. Linh V1!C thuc phiim

a) Phep thif Sinh hec

Phuong phap phat hien va dinhhrong Listeria monocytogenes.PhfuI 2: Phuong phap dinh lUQ11g

D6i tU'Q'Ilgphep thfr


coj/Pham vido

D(>nhay100%

D(>d~c hieu100%

D(>nhay98.8%

D(>d~c hieu99.9%

4HAU

Phuong phap thfr

Gia cam

Tieu chuan co sc

Mas6:TYV6-HDPP.VR-03.01

Tieu chuan cc saMas6:TYV6-HDPP.VR-04.01

OlE Manual.Chuong 2.3.4Mas6:TYV6-HDPP.VR-01.01

Dinh luQ11gEnterobacteriaceae57 bkg phuong phap Slr d\mg rna

d~m Petrifilm

Thit va cac sanpham ill thit, thuysan va san phamthuy san, tnrng vacac san pham tirtnrng, sua tumnguyen lieu, m~tong va cac sanpham nr mat ong

Thit va cac sanphfun ill thit, thuysan va san ph~mthuy san, trll'ng vacac san ph~m illtrlrng, sua tumnguyen li~u, m~tong va cac sanph~m til m~t ong

10 CFU/g1 CFU/ml

10 CFU/g1 CFU/ml

TCVN 7700-2:2007(ISO 11290-2:1998/Amd 1:2004)


TCVN 9980:2013(AOAC 2003.01)Mas6:TYV6-HDPP.VT-11.01

9

1>8i nrongGioi han phat

TT Ten phep thirhien (neu Phuong phap thir

phep thir e6)/Ph~m vido

TCVN 9977 : 2013

Dinh lUQTIgtong s6 vi khu~n hieu Benh pham dong 10 CFU/g Modify

45 khi tu benh pham gia sue gia cam v~t va(AO~C 990.12M)

va thuy san thuy sanMa so:TYV6-HDPP.VTB-14.01

Phat hien khang th~ 16morn longELISA test kit

46 m6ng serotype 0 bang ky thuat Gia sue Hi~u gia 1/40 Mas6:

ELISATYV6-HDPP.VR-02.01

Phat hien khang th~ 16morn longELISA test kit U

47 m6ng serotype A bang kY thuat Gia sue Hi~u gia 1/40 Mas6:

ELISATYV6-HDPP.vR-02.02

Phat hien khang th~ 16 16m long ELISA test kit

48 m6ng serotype Asia 1 bfuIg ky Gia sue Hi~u gia 1140 Mas6:

thuat ELISATYV6-HDPP.VR-02.03

Phat hien khang nguyen vi-rut 16ELISA test kit

49 morn long m6ng bang ky thuat Gia sue 2ng/ml Mas6:

ELISATYV6-HDPP.VR-02.05

Phat hien khang the 3ABC cua vi-DQnhay > ELISA test kit -0

50 rut 16morn long m6ng bang ky Gia sue99% Mas6:

thuat ELISA DQ d~e hieu TYV6-HDPP.VR-

>99%02.04

Dinh lUQTIgkhang th~ khang vi- OlE. Manual.

51rut 16morn long m6ng bang kY Gia sue Hieu gia 1145

Chuong 2.1.5

thuat trung hoa vi rut tren t~ baoMas6:

dQng v~tTYV6-HDPP.VR-02.07

Phful l~p vi-rut 16m6m long m6ng10 TCIDso/ml Tieu chuan CO" sa

52 b,fuIgphuong phap nu6i dy tren Gia sue Mas6:

te bao QQngv~t TYV6-HDPP.VR-02.06

8

TT

35

36

Ten phep thu' D6i nrongphep thif


c6)/Phe;unvido

10 copies/Sill

10 copies/Sill

Phuong phap thif

;~~/ ·"'.I'd'P~(;<>f/

Ph ' hi ~ , , N ~~ _1l t. ~at len VI-rut ewcasuew -0' , G' ~ky thu~t realtime RT-PCR ra cam

Tieu chuan coso

Ma s6:TYV6-HDPP,VR-28.01Tieu chuan co sc

Mas6:TYV6-HDPP.VR-05,02

37Phat hien khang thS vi-rut gaybenh Gumboro bang ky thuatELISA

Gia camHi~u gia

2:. 396

Tieu chuan co So'


38Phat hien vi-rut gay benh vierngan vit bang ky thuat RealtimeRT-PCR

Thuy d.m 10 copies/Sill

Tieu chuan co So'


39Phat hien vi-rut gay benh dich tavit bang ky thuat Realtime PCR Thuy cam 10 copies/Sill

Tieu chuan co So'


40Phat hien vi-rut dai (Rabies virus)bang ky thuat RT-PCR

Ch6, Meo 20 copies/Sui Ma s6:TYV6-HDPP.VR-22.01

Tieu chuan co so'

41Phat hien vi-rut dai (Rabies virus)bang ky thu~t Realtime RT-PCR Ch6, Meo 10 copies/Sill Ma s6:

TYV6-HDPP.VR-22.02

Tieu chuan co So'

Quy trinh nuoi dy va gift gi6ng42 cac dong tS bao

TS baodQng v~t

Tieu chuan co' so'Mas5:TYV6-HDPP.VR-20.01

Ph<ithi~n con trung s6ng bkg43 phuong phap soi tuai tn,rc tiSp

San phfun dQng v~tva thuy san

Tieu chuan ca So'

Mas5:TYV6-HDPP-BL-IS.I

Phat hi~n vi khuan gay b~nh thoi<iutrung ong Chau Au

44 (Melissococcus pluton ius) 60ngnuoi l<iym~t

Ong m~t 10 CFU/g

OIE,2008.Chuang 2.2.3Mas5:TYV6-HDPP.VTB-11.01

7

Gioi han phat

TT Ten phep thfrn8i nrong hien (neu Phuong phap thu'phep thfr co)/Ph(;ll11 vi

dothuat Realtime RT-PCR TYV6-HDPP.VR-

13.01b) Benh don!!vat tren can

PhM hien vi-rut gay benh cumTieu chu§.nco sc

26 subtype N6 bang ky thuat Gia cam, 10 copies/5 ul Mas6Realtime RT-PCR Gia sue TYV6-HDPP.VR-

01.07Tieu chuan co'sa

Phat hien vi-rut cum subtype Gia sue, 10 eopies/5 ulMas6:

27 H7N9 bang kY thuat realtime RT- Gia camTYV6-HDPP.VR-

PCR 01.06

Quy trinh giai trinh nr gen vi-rutTieu chuan co sa

28 cum subtype H5N 1 Gia sue, Mas6:Gia cfun TYV6-HDPP.VR-

01.05Tieu chuan co' sa

29 Phat hien ki sinh trung duong mau Gia sue,bang phuong phap nhuom Giemsa Gia cfun Mas6:

TYV6-HDPP-BL-14.1Tieu chuan coso

Phat hien vi khuan Clostridium Gia sue,30 s1?P.til benh pham gia sue, gia Gia efun10 CFU/g Ma so:

cam TYV6-HDPP.VTB-05.01

Phat hien vi khuful Streptococcus Tieu chuan co sa

31 spp, til benh pham gia sue, gia Gia sue,10 CFU/g Mas6:

cfun, bang phirong phap nuoi cfty Gia earn TYV6-HDPP.VTB-phanl~p 04.01

Tieu chuan co sO-Quy trinh giai trinh tv gen vi-rut

32 La morn long mong serotype 0 va Gia suc Mas6:A TYV6-HDPP.VR-

02.09

Phat hi~n vi-rut PoreineTieu chuan co' so'

33 Circovirus type 2 (PCV2) a lqn Lqn 10 copies/5fl.l Mas6:bfuIg ky thu~t realtime PCR TYV6-HDPP.VR-

21.01Tieu chuan cO'sO-

34 Huang dfuI m6 kh{tm gia sue Gia sue Mas6:TYV6-HDPP-BL-22.1

6

OIE -Aquatic Manual,

10 copies/51l1 2012. Chuong 2.2.3Mas6:TYV6-HDPP.VR-12.01

D6i tuongphep thir

Gi6i han phathien (neu

co)/Ph~m vi Phuong phap thifdo

TT

san pham thuy san,mroc dung trongnang nghiep

Ma so:TYV6-HDPP.VTB-12.06

't'4'~-.._.~,,>JA'Phat hien vi khu~"J.· ~~"i~~

(Milky haemolymph disease) atom hum bang ky thuat PCR

Tom, nhuyen th€,san pham thuy san,mroc dung trongnang nghiep

Tieu chu~nco sc

10 CFU/g Ma s6:TYV6-HDPP.VTB-27.01

18

Phat hien vi-rut gay hQi clnrngd6m trang (WSSV) tren tom bangky thuat Realtime PCR

Cac loaigiap xac

OlE -Aquatic Manual,10 copies/Sui 2012. Chuong 2.2.5


19

OIE -Aquatic Manual,2012. Chuang 2.2.4

10 copies/SuiPhat hien vi-rut gay hQi clnrngTaura (TSV) tren tom bang kYthuat realtime RT-PCR

Cac loaigiap xac

20 Mas6:TYV6-HDPP.VR-11.01

PhM hien vi-rut gay benh diuvang (YHV) tren tom bang kythu~t Realtime RT -PCR

Cac loaigiap xac

OlE -Aquatic Manual,10 copies/Sui 2009. Chirong 2.2.7


21

Phat hien vi-rut gay benh Hoai nrdiroi vo va co quan tao mau(IHHNV) tren tom bang ky thu~tRealtime PCR

Cac loaitom he

OIE -Aquatic Manual,10 copies/Sui 2012. Chuong 2.2.2


22

PhM hi~n vi khuk gay b~nh Ho~iillgan t\ly (NHP-B) tren tom bikgky thu~t Realtime PCR

Cac loaitom he

OlE -Aquatic Manual,10 copies/51l1 2012. Chuang 2.2.4


23

Tom the chanPhat hi~n vi-rut gay b~nh ho~i ill, triing, tom su, tomco (IMNV) tren tom bang ky thu~tRealtime RT-PCR xanh Thai Binh

Duang24

OlE -Aquatic Manual,10 copies/51l1 2013. Chuang 2.3.1125

Phat hi~n vi-rut gay b~nh Ho~i ill Cac loaithin kinh (VNN) tren ca bikg ky ca bi€n

Mas6:

5

Di~u 4. Quyet dinh nay co hieu hrc kS tir ngay ky, Chanh Van phong BQ,Vu tnrong Vu Khoa hoc, Cong nghe va Moi tnrong, Thu tnrong cac co quan, danvi co lien quan va Trung tam chan doan xet nghiem benh dong vat- Co quan thu yvung 6 chiu trach nhiem thi hanh Quyet dinh nay.

Noi nh(in,:- Nhir Dieu 4;- Bo tnrong (d~b/c)- Cac Tfmg cue/Cue thuoc Bo (d~ p/h);- Luu: VT, KHCN. CTLI ifJbdn)

KT. BO TRUONGTmiTRUONG

L8 QuAe Doanh

'-

2

BO NONG NGm~pvA PHAT TRIEN NONG THON

@lI,tlo IQIl-BNN-KHCN

CQNG HOA xA HOI CHiT NGHiA vier NAMDQc l~p - Tl}' do - H~nh phuc

HaN<}i, ngay~thang 10 nom 2015

QUYETDJNHChi dinh Phong thir nghi~m nganh nong nghiep va phat tri~n nong thfm

B(>TRUONG B(>NONG NGm~p vA pIlAT TRIEN NONG THON

Can cir Nghi dinh 199/2013/NI)-CP ngay 26 thang 11 nam 2013 cua Chinhphu quy dinh chirc nang, nhiem vu, quyen han va co cAu t6 chirc cua BQNangnghiep va Phat trien nang than;

Can cir Thong ill s6 16/20111TT-BNNPTNT ngay 01 thang 4 nam 2011 cuaBQNang nghiep va Phat trien nang than quy dinh vS danh gia, chi dinh va quan 1yphong thir nghiem nganh Nang nghiep va Phat trien nang than;

Can cir Thong tu s6 54/2011/TT-BNNPTNT ngay 03 thang 8 nam 2011 cua BQNang nghiep va Phat triSn nang than yeu c~unang hrc phong thir nghiem vS chAthrong,an toan thuc pham Nang lam thuy san va muoi;

Xet Bien ban danh gia phong thu nghi~m cua Trung tam chAndmin xet nghi~mb~nh dQngv~t - Ca quan thil y vUng 6 ngay 09/0112015;

Theo dSnghi cua V\l truang V\l Khoa hQc,Cong ngh~ va Moi truOng,

QUYETDJNH:

Di~u 1. Chi diM l~i va chi diM rna rQng Phong thu nghi~m cua Trung tamChAndoan xet nghi~m b~nh dQngv~t 1aphong thu nghi~m nganh Nang nghi~p vaPhat tri€n nang than v6i rna s6 phong thu nghi~m 1aLAS - NN 11.

f)i~u 2. Phong thu nghi~m neu t~i DiSu 1 duqc tham gia thvc hi~n cac phepthu trong linh VlJC xet nghi~m b~nh dQng v~t, thuc an chan nuoi, thuc an thuy san,an toan thvc ph§.m, nuac dUng trong' nang nghi~p chi ti~t t~i Danh m\lc cac phepthu dinh kern.

f)i~u 3. Phong thu nghi~m neu t~i DiSu I' co trach nhi~m thvc hi~n d~y ducac quy diM neu t~i DiSu 24, Thong ill s6 16/2011/TT-BNNPTNT ngay 01 thang4 nam 2011 cua BQNang nghi~p va Phat tri€n nang than Quy diM vS danh gia, chidinh va quan 1yphong thu nghi~m nganh nang nghi~p va phat triSn nang than.

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Documents

Gioi han phat phepthir D6i thfr coj/Phamvi