Upload
lamthu
View
220
Download
0
Embed Size (px)
Citation preview
A
B
C
1
2
denitrification tank aeration tank
rotating biologicaldiskcontactors
secondaryclarifiers(1– 3)
3
oxidationditch
secondaryclarifier(oxidationditch)
microsieves
effluent
grids andsieves
aerated sludge process (A)rotating biological disk contactors (B)oxidation ditch (C)
influent
aeratedgritchamber primaryclarifier
Biological Wastewater Treatment
A
B
C
1
2
denitrification tank aeration tank
rotating biologicaldiskcontactors
secondaryclarifiers(1– 3)
3
oxidationditch
secondaryclarifier(oxidationditch)
microsieves
effluent
grids andsieves
Arotating biological disk contactors
oxidation ditch
influent
aeratedgritchamber primaryclarifier
BC
aerated sludge process
Mechanical Wastewater Treatment
Results (mechanical wastewater treatment): EEQvaluesoftheinfluentandtheeffluentoftheprimaryclarifiertaking intoaccounttheaveragehydraulicretentiontime(Nov8,2006)
Hormones in the Environment - Quantitation of the Total Estrogenic Activity
of Wastewater Samples with a Bioassay
www.iswa.uni-stuttgart.de
Contact
: Prof.Dr.rer.nat.JörgW.Metzger,ISWA,Bandtäle2,70569Stuttgart
[email protected],www.iswa.uni-stuttgart.de/ch
CorneliaKlaus,BiljanaMarić,AndreaKern,BertramKuchandJörgW.Metzger InstituteforSanitaryEngineering,WasteWaterQualityandSolidWasteManagement,Dept.ofHydrochemistry
Universität Stuttgart
Aim of this Study: • Input/output balance of the total estrogenic activity in the sewage treatment plant (STP) of Stuttgart-Büsnau in Germany • Determination of the efficiencies of purification of the three different biological treatment steps
Material and Methods:Sampling: grabsamplesofeachsewage treatmentsteptakingintoaccount thehydraulicretentiontimeifpossibleinaddition:24hourscomposite samplesofinfluentandeffluent duringoneweekSampleTreatment: centrifugation filtration solidphaseextraction(RP-18)DeterminationofEstrogenicity: biologicalsummaryparameter expressedasanestradiolequivalent concentration(EEQ)inng/LE-Screenassay:invitrocell proliferationassaybasedonthe humanestrogenreceptor-positive breastcancercell-lineMCF-7 generalestimationoftheuncertainty ofmeasurementaspartofthe validationprocessforthesuitabilityof thisbioassay
•
•
•••
•
•
•
Results (24 h composite samples): Diminutionofestrogenicactivityduringwastewatertreatment -EEQvaluesof24hcompositesamples(Nov21-28,2006)
Conclusions:
• TheE-Screenassayisverysuitablefor balancingestrogenicactivityofwastewater samples(datavariationmax.oneorderof magnitude)• EEQ values of the influent rangedbetween 12.6ng/Land72.7ng/L(median23.8ng/L); those of the effluentwerefoundtobe permanentlybelow2.5ng/L(median1.6ng/L)
• Theoveralleliminationefficiencyofthewholeplantisconstantand stable:mechanicalwastewatertreatmentdidnotleadtoany significantelimination,whereasbiologicaltreatmentreducedestrogenicity toagreatextentwithslightdifferencesbetweenthethreetechnologies• Thereisasmallbutconstantinputofestrogenactivecompounds intothereceivingwater:informationonthedilutionfactorandthekindof differentestrogeniccompoundsisrequiredtoevaluateanecotoxicological riskpotential
Results (biological wastewater treatment): Eliminationofestrogenicactivityinwastewater Eliminationofestrogenicactivityinwastewater -activated sludge process -rotating biological disk contactor
Eliminationofestrogenicactivityinwastewater -oxidation ditch
0,0
10,0
20,0
30,0
40,0
50,0
60,0
70,0
80,0
EEQ(ng/L)
influent primaryclarif ier
denitrif icationtank
aerationtank secondaryclarif ier1
secondaryclarif ier2
secondaryclarif ier3
eff luent
Oct11,2006 Oct18,2006 Jan24,2007
0,0
10,0
20,0
30,0
40,0
50,0
60,0
70,0
80,0
influent primaryclarif ier
roll1 roll2 roll3 secondaryclarif ier1
effluent
EEQ(ng/l)
Oct18,2006 Jan10,2007 Jun4,2007
0,0
5,0
10,0
15,0
20,0
25,0
30,0
35,0
40,0
EEQ(ng/L)
influent oxidationditch secondaryclarif ier(OD)
effluent
Oct12/13,2006 Oct17/18,2006 Jan16/17,2007
Reference: Klaus,Cornelia(2007):ErmittlungdesVorkommensundderElimination vonestrogenaktivenSubstanzenimLehr-undForschungsklärwerkStuttgart-Büsnau -BewertungdesE-ScreenAssaysalsinvitro-Testverfahren,diplomathesis,UniversitätStuttgart
→ nosignificant decreasein estrogenicity
→ average efficiencyof removalduring thatweek: 92.2%
Eliminationefficiencies(%)ofbiologicalwastewatertreatment (effluentofthebiologicaltank/rawinfluent)
1.sampling 2.sampling 3.sampling average
activatedsludgeprocess 92.3 97.8 94.4 94.8
rotatingbiologicaldiskcontactors 66.7 82.3 76.3 75.5
oxidationditch 91.0 90.5 93.7 91.7
0,0
5,0
10,0
15,0
20,0
25,0
30,0
35,0
40,0
45,0
50,0
EEQ(ng/L)
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
influent effluent
0
5
10
15
20
25
30
EEQ(ng/L)
7.30a.m./8.55a.m. 7.45a.m./9.10a.m. 8.00a.m./9.25a.m. 8.15a.m./9.40a.m.
inf luent eff luentprimaryclarif ier
samplingsites(grabsamples)