NEW PRODUCT NEWS FROM HOEFER

How to get the most out of your Transfers.

Whether for nucleic acid hybrid-ization, immunostaining or auto-radiography, your transfers mustbe fast and uniform. The currentmust be high and homogeneousthroughout the transfer chamber,and the temperature must be con-trolled. We took these factors intoconsideration when we developedour transfer systems, and as a re-sultproduced electrodepanels, heatexchangers and power supplies togive you the best transfers possible.* The Hoefer Transphor (TE 42) holdsfour standard size gels, 16 x 22 cm, morethan any other unit on the market, or withthe heat exchanger in place, two standardsize gels. Easy-to-load cassettes hold gels

firmly without squeezing. 75% open gridpattern offers maximum transfer. Elec-trodes fixed at 10 cm apart assures a uni-form electrical field. Moving them closertogether results in an inhomogeneous elec-trical field, which in turn causes uneventransfers. The two electrode panels haveplatinum wire (more than 120" of it) strungin a closely spaced parallel pattern to as-sure equal removal of samples. The elec-trode assemblies lock automatically intoplace and can be removed instantly. Twoimportant options: The Power Lid, a lowcost power source which sits directly ontop; and a highly efficient Heat ExchangeManifold which enables you to use higherfield strength for more rapid transfer.Reader service card no. 227* The PS 250 Power Supply is ideal fortransfer electrophoresis, which requireshigh current flow at relatively low voltage.It produces 0- 250V, 0- 2.5A, adjustable inboth constant current and voltage modes.We recommend running transfers in con-stant current to eliminate any possibility ofoverheating. The PS 250 provides amplecurrent for several small or 2 large Hoefer

Transphors, has two digital panel meters, a7 hour timer, and works well with all stan-dard and mini size gel systems.Reader service card no. 2280 The Mighty Small Transphor is idealfor the small gel format. It can transferproteins from 4 small gels (up to 9 x 10 cm)at once, to either nylon or nitrocellulosemembranes-in as little as 30 minutes.The key to successful transfer is a uniformelectrical field between electrodes. Themore platinum wire, properly spaced, themore uniform the field. Mighty SmallTransphor has more than enough platinumwire to achieve this, seven parallel, oppos-ing strands, a total of 115 cm. Spacing ofelectrodes is crucial. They should not betoo close together. We have found thatelectrodes fixed at 10 cm apart assures auniform field. Mighty Small Transphor hasa serpentine cooling channel milled into itsbase and covered with alumina. Used with

a magnetic stirrer and cooling, the unit willshow no more than a 5°C heat rise over thelength of the run.Reader service card no. 229* Hoefer sells Transfer Membranes touse with its Transphor (TE 42) and MightySmall Transphor (TE 22). A pure grade ofTransphor Nitrocellulose Membranes freefrom admixtures of other cellulose estersinsures faithful replicas, maximal bindingand minimal background staining. The

binding capacity is 80-100jig/cm. Highbinding Transphor Nylon 66 Membranesare available in two forms: positivelycharged Nylon 66 Plus (TM-NYP) andstandard Nylon 66 (TM-NYS). We offera wide choice of shapes and pore sizes.Reader service card no. 230

* Deca-Probe is a 10-chamber micro-incubator manifold designed to stain 10separate immunotransfers on a singletransfer membrane. It is a sturdy, precise-ly-engineered unit. Two 19 x 19 cm acrylicplates, each 1.7 cm thick, fit together witha membrane held in place between them.One ofthe plates has parallel troughs milledinto it. These troughs become individualincubation chambers when the plates areclamped together and sealed. An 0-ringgasket surrounding each chamber preventsleakage from one to another, which means

you can use up to 10 separate detectionmethods simultaneously. Reagent volumeis low, normally just enough in each cham-ber to wet the membrane and allow formixing when the unit is rocked. Whenscreening is finished, you remove the mem-brane, intact, for side-by-side comparisonof lanes. No danger of exposure to toxicreagents and radioactivity.* Red Rocker is a solidly built 25 x 25cm rocking platform. We recommend it asan accessory to the Deca-Probe, in fact,for any application where gentle, perfectlyuniform rocking action is required.Reader service card no. 231

* Hoefer Scientific Instruments, P.O.Box 77387, San Francisco, CA 94107USA. In California: 415-282-2307. Out-side California: 800-227-4750. OutsideUSA: Telex 470778.For demonstration of the Transphor line,Reader service card no. 232For complete catalog, Reader servicecard no. 233

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INTERNATIONALCONGRES ONCOMPUTERS IN BIOTECHNOLOGY

JANUARY 30-31, 1986BALTIMORE CONVENTION CENTER, BALTIMORE MARYLAND

Chairman: Stephen R. HellerU.S. Dept. of Agriculture, Agricultural Research Service, Beltsville, Maryland

Computers have and will continue to play a major role in the field of biotechnology, both as a support tool and asresearch tool. As the computer spans a vast range of activities in biotechnology, this conference will endeavor toprovide timely presentations of the policy, resource, system, and applications areas which bear both on the immedi-ate and long term activities in this fast growing, fast moving, and exciting field.

Session TopicsPOLICY ISSUES - Jerrold Roschwalb, National Assoc. of State Univ. & Land Grant CollegesThis session will present the major and critical policy issues in Government support and interaction in the field. A paneldiscussion will follow the session. Speakers will include Dr William Raub (NIH), Dr Rick Weingarten (OTA), and DrDavid Kingsbury (NSF).

SYSTEMS and RESOURCES - Lewis Gevantman, National Bureau of StandardsThis session will present examples of computer systems and developments related to and supporting biotechnology.Speakers will include Professor Carver Mead (CalTech), Professor David Mount (Arizona State), Richard Feldmann(NIH), Dr Kevin Ulmer (CARB), Dr Charlotte Hollister (BBN), and Dr Dennis Smith (Intellicorp).

APPLICATIONS IN AGRICULTURAL BIOTECHNOLOGY - Joseph Modelevsky, International Mineralsand ChemicalsAgricultural biotechnology focuses on problems ranging from the level of the gene to the plant to the farm. Computer-based tools are applied by investigators working on the whole spectrum of problems. This session will address com-puter applications in genetic engineering and protein design, agricultural expert systems and computer-based toolsto optimize production agriculture. Speakers will include Dr Joachim Messing (Rutgers University), Dr RyszardMichalski (University of Illinois), Derek Crates (Imperial Chemical Industries), Dr. James Kendrick (AGNET).

CONTRIBUTED PAPERS - Dieter Soil Yale UniversityContributed papers for the poster and oral presentations are invited. Please request abstract forms from EdwardRuffing, Scherago Associates Inc. (212) 730-1050.

ORGANIZED BY SCHERAGO ASSOCIATES, INC.

Registration Fee: $300.00 Student Fee: $25.00 (Student Status must be in writing) S-11-15Attendance will be limited. Make checks payable to: Scherago Assoc., Inc., Computers in BiotechnologyO1 Please reserve space(s): Registration Fee must be included.E] Please send Registration Application.Cancellations must be received in writing by January 9,1985.NameDept.OrganizationStreet

City State ZipTelephone: ( )

Return to: Computers in Biotechnologyc/o Scherago Associates, Inc.Dept. S-1 1-15, 1515 Broadway, New York, NY 10036 * (212) 730-1050 /D 7/-3 6 / A

IS NOVEMBER 1985 Circle No. 60 on Readers' Service Card 737

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15 NOVEMBER 1985 739

Only the sample breaks down.Brinkmann homogenizers make the difference

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Powerful, durable motors workharder...longer.Brinkmann homogenizers usehn n%,v,_S,,tiihi h n 70r) XAI onn

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For complete information or a demon-stration, call or write:Brinkmann Instruments Co., Division

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This is it-CelliftM.

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Now, right on your benchtop, and with veryminimal investment, you can harvest thebenefits of the latest airlift technology. Youcan set up easily. And you can start cost-effective production immediately.

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Circle No. 237 on Readers' Service Card

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When you check for media con-tamination, Millipore helps you seethe most exciting thing of all: noth-ing. At Millipore, we provide a fullline of sterilizing filtration productsand high performance water purifi-cation systems, backed by the mostaccessible applications support inthe business. All to help you prop-agate even the most sensitive ofcell lines.Sterilizing filtration with virtuallyno protein loss.Millipore filtration products sterilizefrom 1 ml up to 300 liters of mediawith virtually no protein loss. Theexclusive low-extractable Durapore®

membrane is the lowest protein-binding membrane available. Andall filter units are pressure-drivento minimize foaming and the result-ing protein denaturation commonwith vacuum devices.Milli-Q® water maximizes cellgrowth.Studies* document that Milli-Q wa-ter offers both higher growth ratesand higher cell concentrations thaneven double-distilled. The systemprovides pyrogen-free, 1 8-megohmwater-on demand-at up to 1.5liters per minute.Technical Service HOTLINE. Forexpert answers.Just call 1-800-225-1380. Talk di-rectly to our cell culture specialistsabout anything from media

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Extended capability when used with the recirculating water cooler forCOOLING the centrifuge chamber and with the in-line heater for HEATINGthe chamber and holding it at a constant temperature...The better way toachieve greater efficiency and precision when concentrating and meas-urina biologically active compounds.

A TABLE TOP UNIT WITH FLOORMODEL CAPABILITY

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* 12x75 Tubes* 17x95 Tubes* MC Tubes* Mechanical Lock* Variable Speed

The Heat Exchanger coil is a unique feature; circulating controlled No need to go into the cold room since the centrifugetemperature water in order to cool or heat the chamber to prevent can be kept close by on a laboratory bench.samples from overheating...or for the maintenance of temperature Unnecessary noise pollution is eliminated becausebelow or above ambient in order to maintain the stability of the centrifuge is surprisingly quiet by comparisonbiolonicalIv active comoonents. with other wall known makes.

Circle No. 173 on Readers' Service Card

THE IMAGE-i SYSTEM FROM IVS

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A new dimension in microscopy is available. The versatile IMAGE-I

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The easy to use IMAGE- I has been developed in the researchenvironment with leading biologists. All functions have beenextensively tested on laboratory applications.There's a new library of enhancement and analysis

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15 NOVEME.ER 1985 -833