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SI F
SP IR
I I I I I I I I I II I I IFFIFFFI FFIFFFI IFF FFFI FFFIFFFI FFIFFFI FFFIFFFI FFFIFFFI FFFIFFFI FFFIFFFI IFFFI IFFFIIFFI IFFFIFFFFFIFFFFFFFFFFFIFFIFFFIFFFF IFFF IFF S
I I I I RS PI I I SI I
I I I I I I I II I I II II I I I I I I I I
I F S
3267 aa / 354K
3038 aa / 342K
7968 aa / 868K
26926 aa / 2742K
S
SP
I I I I I I I IF
I FSR
P SRS S S SS
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000 11000 12000 13000 14000 15000 16000 17000 18000 19000 20000 21000 22000 23000 24000 25000 26000 27000
I I I I I ICI I I I I I I I I I II I II II I I I I I I I I I I I I I I I II II II I I IIFFFIFFIFFFFFFI IFFI FFIFFF FFF FFFIF IF I FFI FFI FFIFFIFFFIFFFIF
0 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900 2000 2100 2200 2300 2400 2500 2600 2700 2800 2900 3000 3100 3200 3300 3400 3500 3600 3700 3800
I I I II I II I II I I I I IF
I I I I I I I I I I I I I I I IF
1289 aa / 147K
S S GPL L
C
C
C
C
C
S
S
C
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
C
C
C
U
U
U
U
U
U
U
U
P P
D
I FI I I I I I I
D D
D D
K
K
K
K
K
S C
S C
S C
S C
S
S
S
S
S
S
S
C
S
S
AC
C
C
A
A
C
C
C
C
A A A A A A A A A A
P
P
C
C
S
S
S
S
S
S
S
S
S
S
S
C C
C C
C C
F
SP
S
S
C
C
S
S
S
S
S
Trio Family
MLCK Family
CAMK GROUP
CAMK1 Family
CAMK2 Family
CAMKL Family, AMPK Subfamily
CAMKL Family, BRSK Subfamily
CAMKL Family, CHK1 Subfamily
CAMKL Family, HUNK Subfamily
CAMKL Family, LKB Subfamily
CAMKL Family, MARK Subfamily
CAMKL Family, MELK Subfamily
CAMKL Family, NIM1 Subfamily
CAMKL Family, NuaK Subfamily
CAMKL Family, PASK Subfamily
CAMKL Family, QIK Subfamily
CAMKL Family, SNRK Subfamily
CAMK-Unique Family
CASK Family
DAPK Family
DCAMKL Family
MAPKAPK Family, MAPKAPK Subfamily
MAPKAPK Family, MNK Subfamily
MLCK Family
PHK Family
PIM Family
PKD Family
PSK Family
RAD53 Family
Trbl Family
TSSK Family
Obscn Q5VST9
SPEG Q15772Trad Q9Y2A5TRIO O75962
TTN Q4ZG20
CaMK1α Q14012CaMK1β Q6P2M8CaMK1δ Q8IU85CaMK1γ Q96NX5CaMK4 Q16566VACAMKL Q8WTT8
CaMK2α Q9UQM7CaMK2β Q13554CaMK2δ Q13557CaMK2γ Q13555
AMPKα1 Q13131AMPKα2 P54646
BRSK1 Q8TDC3BRSK2 Q8IWQ3
CHK1 O14757
HUNK P57058
LKB1 Q15831
MARK1 Q9P0L2MARK2 Q7KZI7MARK3 P27448MARK4 Q96L34
MELK Q14680
NIM1 Q8IY84
NuaK1 O60285NuaK2 Q9H093
PASK Q96RG2
QIK Q9H0K1QSK Q9Y2K2SIK P57059
SNRK Q6IQ46
SgK495 Q96I44STK33 Q8NEF5
CASK O14936
DAPK1 P53355DAPK2 Q9UIK4DAPK3 O43293DRAK1 Q9UEE5DRAK2 O94768
DCAMKL1 O15075DCAMKL2 Q8N568DCAMKL3 Q9C098
MAPKAPK2 P49137MAPKAPK3 Q16644MAPKAPK5 Q8IW41
MNK1 Q9BUB5MNK2 Q9HBH9
caMLCK Q32MK0SgK085 Q86YV6skMLCK Q9H1R3smMLCK Q15746
PHKγ1 Q16816PHKγ2 P15735
PIM1 P11309PIM2 Q9P1W9PIM3 Q86V86
PKD1 Q15139PKD2 Q9BZL6PKD3 O94806
PSKH1 P11801PSKH2 Q96QS6
CHK2 O96017
Trb1 Q96RU8Trb2 Q92519Trb3 Q96RU7
SSTK Q9BXA6TSSK1 Q9BXA7TSSK2 Q96PF2TSSK3 Q96PN8TSSK4 Q6SA08
CS I
370 aa / 41K343 aa / 38.5K
385 aa / 43K476 aa / 53K473 aa / 52K
501 aa / 54K
478 aa / 54K664 aa / 73K
499 aa / 56K558 aa / 63K
794 aa / 87K
550 aa / 63K552 aa / 62K
736 aa / 82K
433 aa / 49K
476 aa / 54K
795 aa / 89K
714 aa / 80K
788 aa / 88K776 aa / 87K
752 aa / 82.5K
436 aa / 50K
651 aa / 75K
1323 aa / 143K
661 aa / 74K628 aa / 70K
926 aa / 104K1371 aa / 140K783 aa / 85K
765 aa / 84K
435 aa / 49K514 aa / 58K
926 aa / 105K
370 aa / 43K454 aa / 52.5K
414 aa / 47K372 aa / 42K
1431 aa / 160K
740 aa / 82K766 aa / 83.6K
648 aa / 74K
400 aa / 46K382 aa / 43K
473 aa / 54K
819 aa / 88K
465 aa / 51K418 aa / 47K
388 aa / 44.5K595 aa / 65K
386 aa / 45K406 aa / 46K
1914 aa / 211K
404 aa / 45K311 aa / 34K326 aa / 36K
912 aa / 102K878 aa / 97K890 aa / 100K
372 aa / 41K
423 aa / 48K385 aa / 43K
343 aa / 39K
543 aa / 61K
358 aa / 40K
273 aa / 30K367 aa / 42K358 aa / 41K
268 aa / 30K328 aa / 37.5K
xplxRxxSxxxxxxx
xxlsRqxSldxxxxx
xxlrRsxSfxxLxxl
xxxxxxxSxcxxxxx
xflxRxxSxxxxxxx
xxgxxLxTxCGsppY
xvlsKxgSxxnlkhx
xxxxxxxSxxxxlxr
xxRxRRxSxxfxxxx
xxLxRspSlxxxxxx
xxxxsssStxxxggx
xxxxxxxSxrxxxxx
xxRxRrxSxxxxxxx
xpLxRrxSxvxxxxx
pxlxRxxSqxxkxxx
xxxxxxxSxxxsxxx
kkrKxxxSxrxxxxx
KinaseNET - The Human Kineome Knowledgebase
0 100 200 300 400 500 600 700 800 0 100 200 300 400 500 600 700 800 0 100 200 300 400 500 600 700 800
LEGEND
BBC B
BBOX B
BTK B
DCX D
DnaJ D
DSRM D
EGF E
E
FATC F
FCH F
FHA F
FN3 F
C1 C
C2 C
CNH C
cNMP C
ABC1 A
ADF A
Activin_recp A
Alpha_kinase A
ANF_receptor A
ANK A
B41 B
BROMO B
Coiled-coil C
CARD C
Calmodulin-bind C
Arm A
FAST_Leu-rich F
FAT F
CYCc C
FA58C F
EPH_lbd E
Focal_AT F
DEATHD
COR C
Extensin_2 E
Autoinhibitory A
CaMK2 assoc. C
Fz F
GS G
HEAT H
If I
IGc2 I
IQ I
KA1 K
L27 L
LDLa L
LIM L
LRR L
LRRCT L
LRRNT L
LY L
PB1 P
PHD P
PI3Kc P
PH P
HR1 H
PBD P
PDZ P
HGTP_anticodon H
MIT M
Ion_trans I
IG I
IG_like I
IGv I
HSP20 H
HATPase_c H
NUC194 N
PAS P
GuKc G
MYSc M
MAM M
GCC2_GCC3 G
KR K
FFurin-likePSI P
PX P
RBD R
Recep_L_domain R
Rhodanese R
Ribonucl_2-5A
R
RIO R
RGS R
RhoGAP R
RhoGEF R
RING
PQQ P
RCC1 R
FU F POLO_box P
Pkinase_C P
Specific information on these domains can be obtained from www.pfam.wustl.edu/browse.shtml and www.smart.embl- heidelberg.de/browse.shtml. The concensus phosphorylation specificity for representatives of the various protein kinase groups are shown here in yellow text with the key amino acids residues indicated in capital letters. More detailed information for each of these protein kinases is available on-line from Kinexus at www.kinaseNET.ca. Phosphorylation site information for these protein kinases and at least 10,000 substrate poteins can be retrieved from Kinexus at www.phosphoNET.ca.
36
4
4
127
621
196
329
124
5
411
65
59
6
15
141
1
20
3
15
5
2
14
2
6
2
2
5
4
9
11
91
3
314
72446
12916
22
7
143
715
11
37
1
5
2
45
59
24
2
2
54
2
4
1
1
6
6
53
3
11
32
31114
1
4
71
R
75-102 (91)129-130 (130)199-231 (213)
28-35 (31)
6-41 (27)
42-48 (45)
37 (37)
104-137 (114)
128 (128)
175-316 (287)117-124 (120)21-793 (58)369-401 (385)195-196 (196)
66-67 (67)
137-157 (147) 168 (168)324-450 (385)
69-210 (87)138-139 (139)
28-33 (32)
62 (62)
41 (41)
357-401 (379)
36-52 (49)
92--94 (93)
228-255 (240)127 (127)
64 (64)
76-97 (86)
73-124 (90)
102-117 (114)
335-364 (348)
46-57 (52)
26-37 (33)
92 (92)
11-26 (20)
196-233 (177)
84-92 (88)
138-142 (139)43-50 (45)29-31 (31)
124-141 (129)
92 (92)
17 (17)
56-108 (76)
58-151 (80)
23-30 (28)
83 (83)54-56 (55)37 (37)52-55 (54)19-25 (23)
157 (157)
68 (68)80-84 (81)14-74 (48)
289-332 (306)46-80 (65)
76-89 (81)
39-43 (41)
32-41 (37)
398 (398)
64-72 (68)
90-130 (121)
64-85 (71)93 (93)
44-62 (49)
28-35 (32)
62-183 (86)
153-162 (156)
173 (173)
53-102 (69)
29-79 (54)
48-50 (49)
206-213 (210)
702-723 (713)
45-49 (47)
44 (44)108-113 (110)73-76 (74)
112-122 (116)
107 (107)
127-132 (129)
224-249 (237)
119-122 (121)
185 (185)176-189 (180)
50-75 (60)
31-39 (34)
39-65 (53)
RWD R
SAM_1 S
SEC14 S
Sema S
SH2 S
SH3 S
signal_peptide S
RRM R
SARAH S
SAM S
11
12
30
2
16
7829
1
113 (113)
65 (65)
146 (146)445-446 (446)76-106 (86)53-68 (59)16-56 (26)
83 (83)
48 (48)
64-75 (68)
SWIM S
TPR_1 T
tRNA-synt_2b T
TUDOR T
WD40 W
WH2 W
TyrKinase T
SerThrKinase S
TMD T
WIF W
UBA U
UME U
SPEC S
TBC T
TTIG
39911
93121
13
11
1
6
1
4
82
1
29 (29)
34 (34)165 (165)59 (59)
32-61 (45)20 (20)
246-359 (269)
192-574 (270)
18-24 (23)
129 (129)
38-45 (40)107 (107)
107-108 (108)
212 (212)83-95 (90)
RROC 2 121-222 (172)
Freq. Freq. Freq.a.a. lengthlow-high (average)
a.a. lengthlow-high (average)
a.a. lengthlow-high (average)
0 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500
II
TS TF FI I
S
S
S
S
SS
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
B
B
S
T
T
T
P
P
P
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
C
C C
C C
U
U
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
T
SS
S
S
S
S
S
EL M
S
S
S
S
S
S
S
S
S
F F
F F
I I
I I I I I I I
F F
F F
F
F F
F
F
F
F
F
F
F
F
F F
F
F
F
F
F
F
F
F
F
F F
F
F
F
F
FF
F F F F F F
FF F
FF F
I I
I
I
I
I
I
II
I
I
I I I
I
I
I
I
II
I I
I
I
I
I
I I
I
I
I
I
I
I
I
I
I
I
I
I
F F
F F
F F F
F F
F
F
F
F
R
R
R
R
R
R
R
R
FR R
FR
FR R
R
E G
E
E
E
E
E
E
E
E
E
E
E
E
G
G
G
G
G
G
G
G
G
G
G
S
S
S
S
S
S
S
S
S
S
S
S
B
B
F
F
S
S
P
P T T T
T T T
F
F
F
C
K
L
K
L L
W
B
B
B
E EE
E E E
L
L L L
L L
L LL
S
F
F
I
I I
I I
I I
I
I I
I I
I
I
I I
I I
I I
L
L
L
T
S T
T
T
T
T
T
T
T
T
T S
T
T
S
T F
FT
T
T
I T
T
T
T
B T
B T T
T
T
T
T
T
T
T
T
T
TSS
T
T
S T
T
T
T
T
T
T
T
P B T
TS
TS
S
T
T
T
TS TF FGE S
S
T
S
G
1149 aa / 123K1182 aa / 128K
1036 aa / 114K666 aa / 72.5K
1620 aa / 176K
864 aa / 92K
890 aa / 97K887 aa / 97K
999 aa / 110K
1070 aa / 118K
450 aa / 51K507 aa / 56.5K
913 aa / 101K855 aa / 97K
1210 aa / 134K1255 aa / 138K
1342 aa / 148K
1308 aa / 147K976 aa / 108K976 aa /108K983 aa / 110K986 aa / 110K
1037 aa / 115K1036 aa / 115K
998 aa / 112K1005 aa / 111K1008 aa / 110K
984 aa / 110K1055 aa / 117.5K
998 aa / 110K987 aa / 108K1006 aa / 109K
1009 aa / 116K1052 aa / 119K
822 aa / 95K822 aa / 93.5K
821 aa / 92K822 aa / 92K
806 aa / 88K802 aa / 88K
1367 aa / 155K1382 aa / 156K
1297 aa / 144K
1142 aa / 132K1132 aa / 131K1124 aa / 125K
1326 aa / 140K
1503 aa / 165K
1480 aa / 156K1390 aa / 155.5K
1400 aa / 152K869 aa / 97K
993 aa / 113K972 aa / 108K976 aa / 110K
1089 aa / 123K1106 aa / 124K
1114 aa / 124K
937 aa / 104K943 aa / 105K
604 aa / 67.5K2347 aa / 264K
504 aa / 58K451 aa / 52K
529 aa / 59.5K505 aa / 58K
536 aa / 61K525 aa / 59.5K
508 aa / 58K511 aa / 58K535 aa / 60K
488 aa / 54.5K542 aa / 61K
635 aa / 72K619 aa / 70K
675 aa / 78K659 aa / 76K
620 aa / 72K631 aa / 74K
527 aa / 61K
1138 aa / 125K1124 aa / 126K
422 aa / 48K
796 aa / 87.5K822 aa / 92K839 aa / 94.5K
1338 aa / 151K
1298 aa / 146K
1356 aa / 151.5K
1187 aa / 134K
F TF F T
xxxndxxYxxPxxxx
xxxedxxYvnfxxxx
xxxexxdYdxxxxxx
xxxxxxxYxexxxxx
ftatexqYqpqxxxx
xxxxexxYxxxxxxx
xxxxxxxYxxxpxxx
xxxexddYmxMxxxx
xxxxIdxYIkIxxxx
xxdxdxxYvxvxxxx
xxxedxnYvxxxpxx
txxxxxxYxxxxxxr
xxxeedvYgxvxxxx
xxxxxxxYxxxxgxx
xeddddDYExpxexx
xxxxxxlYxxxxxxx
xxxxxxxYxxxtxxx
xgxxxdyYyxxggxx
xxsxxxdYxxxxxxx
TK GROUP
Abl Family
Ack Family
Alk Family
Axl Family
CCK4 Family
Csk Family
DDR Family
EGFR Family
Eph Family
Fak Family
Fer Family
FGFR Family
InsR Family
Jak Family
Lmr Family
Met Family
Musk Family
PDGFR Family
Ret Family
Ror Family
Ryk Family
Sev Family
Src Family
Syk Family
Tec Family
Tie Family
TK-Unique Family
Trk Family
VEGFR Family
ABL P00519ARG P42684
ACK1 Q07912TNK1 Q13470
ALK Q9UM73LTK P29376
TYRO3 Q06418AXL P30530MER Q12866
CCK4 Q13308
CSK P41240CTK P42679
DDR1 Q08345DDR2 Q16832
EGFR P00533ErbB2 P04626ErbB3 P21860ErbB4 Q15303
EPHA1 P21709EPHA2 P29317EPHA3 P29320EPHA4 P54764
EPHA5 P54756EPHA6 Q9UF33EPHA7 Q15375EPHA8 P29322
EPHB1 P54762EPHB2 P29323EPHB3 P54753EPHB4 P54760EPHB6 O15197
FAK Q05397PYK2 Q14289
FER P16591FES P07332
FGFR1 P11362FGFR2 P21802FGFR3 P22607FGFR4 P22455
IGF1R P08069INSR P06213IRR P14616
JAK1 P23458JAK2 O60674JAK3 P52333TYK2 P29597
LMR1 O75136LMR2 Q8IWU2LMR3 Q96Q04
MET P08581RON Q04912
MUSK O15146
FLT3 P36888FMS P07333KIT P10721PDGFRα P16234PDGFRβ P09619
RET P07949
ROR1 Q01973ROR2 Q01974
RYK P34925
ROS P08922
BLK P51451BRK Q13882FGR P09769FRK P42685FYN P06241HCK P08631LCK P06239LYN P07948SRC P12931SRM Q9H3Y6YES P07947
SYK P43405ZAP70 P43403
BMX P51813BTK Q06187ITK Q08881TEC P42680TXK P42681
TIE1 P35590TIE2 Q02763
SuRTK106 Q6J9G0
TRKA P04629TRKB Q16620TRKC Q16288
FLT1 P17948FLT4 P35916KDR P35968
EPHA10 Q5JZY3
0 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500
SA
D
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
TKL GROUP
IRAK Family
LISK Family, LIMK Subfamily
LISK Family, TESK Subfamily
LRRK Family
MLK Family, HH498 Subfamily
MLK Family, ILK Subfamily
MLK Family, LZK Subfamily
MLK Family, MLK Subfamily
MLK Family, TAK1 Subfamily
RAF Family
RIPK Family
STKR Family, Type 1 Subfamily
STKR Family, Type 2 Subfamily
TKL Family, Unique Subfamily
IRAK1 P51617IRAK2 O43187IRAK3 Q9Y616IRAK4 Q9NWZ3
LIMK1 P53667LIMK2 P53671
TESK1 Q15569TESK2 Q96S53
LRRK1 Q38SD2LRRK2 Q5S007
HH498 Q59H18
ILK Q13418
DLK Q12852LZK O43283
MLK1 P80192MLK2 Q02779MLK3 Q16584MLK4 Q5TCX8ZAK Q9NYL2
TAK1 O43318
ARAF P10398BRAF P15056KSR1 Q8IVT5KSR2 Q6VAB6RAF1 P04049
ANKRD3 P57078RIPK1 Q13546RIPK2 O43353RIPK3 Q9Y572SgK288 Q8NFD2
ALK1 P37023ALK2 Q04771ALK4 P36896ALK7 Q8NER5BMPR1A P36894BMPR1B O00238TGFβR1 P36897
ACTR2A P27037ACTR2B Q13705BMPR2 Q13873MISR2 Q16671TGFβR2 P37173
MLKL Q8NB16
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C C
AA A
AA A A A A A A A A
AA A A A A A A A A A
C
AA A
AA A AA A AA A A
WW W W W W
S
S
S
S
S
S
S
S
T
T
T
T
T
T
T
T
T
T
T
T
S
S
S
S
R
L L
L L
P
P
D
D
D
LL L L L L L L
LL LL L L L LL
C
S
R
R
R
D
A G
A G
A G
A G
A G
A G
S
S
S
S
A
A
A
A
I
C
S
S
S
S
S
S
S
S
S
G
S
C C C C
R
712 aa / 76.5K590 aa / 65K596 aa / 68K
460 aa / 51.5K
647 aa / 73K638 aa / 7K
2053 aa / 225K
626 aa / 68K571 aa / 64K
2527 aa / 286K936 aa / 104K
452 aa / 51K
859 aa / 93K966 aa / 108K
847 aa / 93K
1104 aa / 122K954 aa / 104K
1036 aa / 114K800 aa / 91K
606 aa / 67K
606 aa / 68K766 aa / 84K
921 aa / 102K829 aa / 94K
648 aa / 73K
832 aa / 92K671 aa / 76K
540 aa / 6K518 aa / 57K
765 aa / 85K
505 aa / 57K
503 aa / 56K509 aa / 57K
493 aa / 55K532 aa / 60K
502 aa / 57K503 aa / 56K
513 aa / 58K512 aa / 58K
1038 aa / 115K573 aa / 63K567 aa / 64.5K
471 aa / 54.5K
xxxxxxxSxxvxxtx
xxxxxxaSgvxvxdx
rxrfxqfSyxasxxe
kxxxxxxTxxxxxxx
lxxxxxkTxaxxxxx
xxLrxtxSxxxxxxx
xxrsrrxSxxxxxxx
xxxpxxxSxxxxxxx
xxxdxxxSgxxlxIx
F
CH
H
H F
F
F
F
N
T
F
F
F
F
P
P
P
P
P
FP
3056 aa / 351K
2644 aa / 301K
4128 aa / 469K2549 aa / 289K
3657 aa / 410K
3859 aa / 438K0 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900 2000 2100 2200 2300 2400 2500 2600 2700 2800 2900 3000 3100 3200 3300 3400 3500 3600 3700 3800
C
C
C
C
P
A
A
A
A
A
S
S
T T
T T
I
I
A
A
R
I
D
F
D
D
C
C
H
H
H
H
H
H
C
B
BB
B
BB
B B
R
A
A
AI
AC I
C C
C
P
BB BR
R B
P
B
R
PB
B P BB B
B
B
B
B
R
R
R
B
B
Atypical GROUP
ABC1 Family, ABC1-A Subfamily
ABC1 Family, ABC1-B Subfamily
ABC1 Family, ABC1-C Subfamily
Alpha Family, ChaK Subfamily
Alpha Family, eEF2K Subfamily
Alpha Family, Unique Subfamily
BCR Family
BRD Family
FAST Family
G11 Family
H11 Family
PDHK Family
RIO Family, RIO1 Subfamily
RIO Family, RIO2 Subfamily
RIO Family, RIO3 Subfamily
TAF1 Family
TIF1 Family
PIKK Family, ATM Subfamily
PIKK Family, ATR Subfamily
PIKK Family, DNAPK Subfamily
PIKK Family, FRAP Subfamily
PIKK Family, SMG1 Subfamily
PIKK Family, TRRAP Subfamily
ADCK3 Q8NI60ADCK4 Q8TAJ1
ADCK1 Q6PD65ADCK5 Q3MIX3
ADCK2 Q7Z695
ChaK1 Q96QT4ChaK2 Q9BX84
eEF2K O00418
AlphaK1 Q96QP1AlphaK2 Q86TB3
AlphaK3 Q96L96
BCR P11274
BRD2 P25440BRD3 Q15059BRD4 O60885BRDT Q58F21
FASTK Q14296
G11 P49842
H11 Q9UJY1
BCKDK O14874PDHK1 Q15118PDHK2 Q15119PDHK3 Q15120PDHK4 Q16654
RIOK1 Q9BRS2
RIOK2 Q9BVS4
RIOK3 O14730
TAF1 P21675TAF1L Q8IZX4
TIF1α O15164TIF1β Q13263TIF1γ Q9UPN9
ATM Q13315
ATR Q13535
DNAPK P78527
FRAP P42345
SMG1 Q96QV0
TRRAP Q9Y4A5
C
H H H H
H
H
H H H H H
H H H
H H H H
H
H
H H
H
H
H H
F
U
647 aa / 72K503 aa / 56K
523 aa / 60K580 aa / 66K
626 aa / 69K1865 aa / 213K
726 aa / 79.5K 1362 aa / 152K
947 aa / 108K
549 aa / 61K
368 aa / 41K
196 aa / 22K
552 aa / 63K
519 aa / 59K
412 aa / 46K436 aa / 49K
407aa / 46K406 aa / 47K411 aa / 46K
568 aa / 66K
2022 aa / 232K725 aa / 82K
1907 aa / 201K
2170 aa / 237K
1244 aa / 139K
1271 aa / 143K
801 aa / 88K
1872 aa / 213K
1826 aa / 207K1050 aa / 117K
1127 aa / 122.5K835 aa / 89K
xxxxxxxSQxxxsxx
xxxexxlSQgxxxxx
xqxsxqxSQxxxxxx
xtxxxpxSPxaxxxx
xxxxxxxSxxkxxxx
xxxxxgxSxxxxxxs
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
SS
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
K O14976
S
C
C C
C
C
C
C
C
C
C
C
C
C C C
C
C
C
C
C
C
W W W W W W
S
S C
A
A A A A A AA A
A A A
A
M M
S
S
S
T
T
T
T
R
R
P P P P P
P P P P
P P
R R R R
R R R R
R
R
T
T
R
D D
P P
P P
P P
H H H H
H H
H H
W
T R
H H H H
H H
D
P
S
S
S
S
S
S
S
S
C
S
S
S
S
S
S
S
SC
C
C
C
R
C
C
C
H H
W
C
S
H
C
C
403 aa / 46K344 aa / 39K
309 aa / 36K
1050 aa / 120K1085 aa / 122K
253 aa / 28K
505 aa / 56K588 aa / 65K
574 aa / 64K
391 aa / 45K350 aa / 41K
798 aa / 88K
756 aa / 86.5K745 aa / 85K
716 aa / 80K729 aa / 84K
977 aa / 110K925 aa / 102K
346 aa / 38K
841 aa / 95K708 aa / 81K
313 aa /36K302 aa / 35K
692 aa / 75K979 aa / 107K
1175 aa / 133K645 aa / 74K
424 aa / 46K935 aa / 102K
581 aa / 63K1383 aa / 147K
1746 aa / 193K401 aa / 45K
341 aa / 39K
1451 aa / 163K485 aa / 52K
535 aa / 60K294 aa / 34K
419 aa / 46.5K741 aa / 83.5K
680 aa /76K350 aa / 40K
1019 aa / 116K294 aa /33KK
929 aa /105K
1649 aa / 187K1115 aa / 125K
630 aa / 71K551 aa / 62K
603 aa / 68K685 aa / 78K
646 aa / 72K970 aa / 109K
808 aa / 90K929 aa / 104K
741 aa / 83K
578 aa / 65K
893 aa / 101K
766 aa /87K772 aa / 88K
322 aa / 36K
841 aa / 95K1315 aa / 144K
1050 aa / 113K1036 aa / 113K
472 aa / 53K
1243 aa / 135K
1743 aa / 192K2297 aa / 243K
2382 aa / 251K567 aa / 63K
646 aa / 72K499 aa / 54.5K
1357 aa / 153K
580 aa / 65K
506 aa /58K445 aa / 52K
1258 aa / 143K
304 aa / 34.5K1311 aa / 143K
1161 aa / 129K897 aa / 94K
xxxxrRrSxxxxxxx
xxxxrxxSxxxsxxx
xxxeeedSDdEeeee
xxdxxxxSlxdxxxx
xxxxfxxSxxxxxxx
xxxxxxxSPxxxxxx
xxxxxkxTxxxrxxx
xxxxxdxSxxxrxxx
xxxxxexSxxxrxxx
xrxvxxkSxxgtxxx
Other GROUP
AUR Family
BUB Family
Bud32 Family
CAMKK Family, Meta Subfamily
CDC7 Family
CK2 Family
Haspin Family
IKK Family
IRE Family
MOS Family
NAK Family
NEK Family
NKF1 Family
NKF2 Family
NKF3 Family
NKF4 Family
NKF5 Family
NRBP Family
Other-Unique Family
PEK Family, GCN2 Subfamily
PEK Family, PEK Subfamily
PEK Family, Unique Subfamily
PLK Family
SCY1 Family
Slob Family
TBCK Family
TLK Family
TOPK Family
TTK Family
ULK Family
VPS15 Family
WEE Family
Wnk Family
AurA O14965AurB Q96GD4AurC Q9UQB9
BUB1 O43683BUBR1 O60566
PRPK Q96S44
CaMKK1 Q8N5S9CaMKK2 Q96RR4
CDC7 O00311
CK2α1 P68400CK2α2 P19784
Haspin Q8TF76
IKKα O15111IKKβ O14920IKKε Q14164TBK1 Q9UHD2
IRE1 O75460IRE2 Q76MJ5
MOS P00540
AAK1 Q2M2I8BIKE Q9NSY1GAMPSK1 O75716
NEK1 Q96PY6NEK2 P51955NEK3 P51956NEK4 P51957NEK5 Q6P3R8NEK6 Q9HC98NEK7 Q8TDX7NEK8 Q86SG6NEK9 Q8TD19
SBK Q52WX2SgK069 XP_372749
PINK1 Q9BXM7
SgK223 Q86YV5
SgK269 Q8NCM3
CLIK1 Q8TDR2CLIK1L Q8N165
SgK307 Q7RTP3SgK424
NRBP1 Q9UHY1NRBP2 Q8NCX8
KIS Q8TAS1RNASEL Q05823SgK071 Q8NE28SgK196 Q9H5K3SgK396 Q9BXU1SgK493 Q504Y2SgK496 Q6IN87
GCN2 Q9P2K8
PEK Q9NZJ5
HRI Q9BQI3PKR P19525
PLK1 P53350PLK2 Q9NYY3PLK3 Q9H4B4PLK4 O00444
SCYL1 Q96KG9SCYL2 Q6P3W7SCYL3 Q8IZE3
Slob Q7Z7A4
TBCK Q8TEA7
TLK1 Q9UKI8TLK2 Q86UE8
PBK Q96KB5
TTK P33981
Fused Q8TC32ULK1 O75385ULK2 Q8IYT8ULK3 Q9UFS4ULK4 Q96C45
PIK3R4 Q99570
MYT1 O14731WEE1 P30291WEE1B P0C1S8
Wnk1 Q9H4A31Wnk2 Q9Y3S1Wnk3 Q9BYP7Wnk4 Q96J92
NEK10 Q6ZWH5NEK11 Q8TBY1
0 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500
0 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
C
C
C
C
C
C
C
C C
C
C C
C
C
C
C C
C
C
C
C
P
P
P
C
C
C
C
C
C
C
S C C
RS
S
S
I I
I IM
M
S
S
S
S
S
S
S
S
S
S
S
I
P S
S
S
S
S
S
S
S
S
S
S
C
C C
C
C
C
C CC
C
P
P
P
P
P
CC C CC
C
C C C C C C C C C
C
S
467 aa / 53K947 aa / 104K
1495 aa / 164K
618 aa / 69.5K626 aa / 71K
1607 aa / 182K
1374 aa / 154.5K
1011 aa / 112.5K1313 aa / 147K
1328 aa / 150.5K527 aa / 58K547 aa / 60K
819 aa / 92K833 aa / 91K846 aa / 95K
894 aa / 101K1239 aa / 142K
1360 aa / 155K1332 aa / 150K
1384 aa / 156K487 aa / 55K491 aa / 56K 1615aa / 186K
1341 aa / 152K
545 aa / 61K524 aa / 58K
559 aa / 62K
591 aa / 64K719 aa / 81K
681 aa / 75K
968 aa / 112K1235 aa / 143K
431 aa / 48.5K418 aa / 47K
1001 aa / 116K1235 aa / 138K
898 aa / 105K
443 aa / 49K416 aa / 46.5K426 aa / 48K
392 aa / 43K400 aa / 44K
347 aa / 39K399 aa / 44K
438 aa / 49K334 aa / 37.5K
419 aa / 47.5K
hgxxxxLYxYxrxYR
xxdxxxxSlxxxxxg
xxgslaxTxaxxxsx
xxtxxxxTfxxxtxx
xxxxxxxTlxrxxxx
xxkxxxkTlkxxxxx
xtxxRRrSvxgxlxx
xxxxrrxSxxxxxxx
xxxxxxxTxrxxxxx
xxxxxxxTxrrxxxx
STE GROUP
STE Family, Unique Subfamily
STE11 Family
STE20 Family, FRAY Subfamily
STE20 Family, KHS Subfamily
STE20 Family, MSN Subfamily
STE20 Family, MST Subfamily
STE20 Family, NinaC Subfamily
STE20 Family, PAKA Subfamily
STE20 Family, PAKB Subfamily
STE20 Family, SLK Subfamily
STE20 Family, STLK Subfamily
STE20 Family, TAO Subfamily
STE20 Family, YSK Subfamily
STE7 Family
COT P41279NIK Q99558
MEKK1 Q13233MEKK2 Q9Y2U5MEKK3 Q99759MEKK4 Q9Y6R4ASK1 Q99683ASK2 O95382MAP3K15 Q5JPR4YSK4 Q56UN5
OSR1 Q3LR53STLK3 Q9UEW8
GCK Q12851HPK1 Q92918KHS1 Q9Y4K4KHS2 Q8IVH8
ZC1 O95819ZC2 Q9UKE5ZC3 Q8N4C8ZC4 Q5H9K2
MST1 Q13043MST2 Q13188
MYO3A Q8NEV4MYO3B Q8WXR4
PAK1 Q13153PAK2 Q13177PAK3 O75914
PAK4 O96013PAK5 Q9P286PAK6 Q9NQU5
LOK O94804SLK Q9H2G2
STLK5 Q9H272STLK6 Q9C0K7
TAO1 Q7L7X3TAO2 Q9UL54TAO3 Q9H2K8
MST3 Q9Y6E0MST4 Q9P289YSK1 O00506
MEK1 Q02750MEK2 P36507MEK3 P46734MEK4 P45985MEK5 Q13163MEK6 P52564MEK7 O14733
1210 aa / 131K1198 aa / 131K1215 aa / 134K
1490 aa / 164K
1512 aa / 165K
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
CMGC GROUP
CDK Family, CDC2 Subfamily
CDK Family, CDK10 Subfamily
CDK Family, CDK4 Subfamily
CDK Family, CDK5 Subfamily
CDK Family, CDK7 Subfamily
CDK Family, CDK8 Subfamily
CDK Family, CDK9 Subfamily
CDK Family, CRK7 Subfamily
CDK Family, PITSLRE Subfamily
CDK Family, TAIRE Subfamily
CDK Family, Unique Subfamily
CDKL Family
CLK Family
DYRK Family, Dyrk1 Subfamily
DYRK Family, Dyrk2 Subfamily
DYRK Family, HIPK Subfamily
DYRK Family, PRP4 Subfamily
GSK Family
MAPK Family, ERK Subfamily
MAPK Family, ERK7 Subfamily
MAPK Family, JNK Subfamily
MAPK Family, nmo Subfamily
MAPK Family, p38 Subfamily
RCK Family
SRPK Family
S
S
C
C
C
C C
C
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
623 aa / 71K419 aa / 48K
533 aa / 59K826 aa / 92K
688 aa / 77.5K
359 aa / 41K372 aa / 42K365 aa / 42K367 aa / 42K
632 aa / 71K
544 aa / 60K
427 aa / 48K424 aa / 48K464 aa / 53K
515 aa / 57K
379 aa / 43K360 aa / 41K
721 aa / 83K557 aa / 63K
815 aa / 89K
1007 aa / 117K
616 aa / 69K
483 aa / 51K420 aa / 47K
588 aa / 66K520 aa / 60K
490 aa / 60K481 aa / 57.5K
763 aa / 86K629 aa / 69K
592 aa / 67.5K315 aa / 36K
1030 aa / 115.5K
484 aa / 57K499 aa / 60K
384 aa / 44K
346 aa / 39K
469 aa / 53K
357 aa / 42K493 aa / 56K
780 aa / 91K
496 aa / 56K523 aa / 60K
472 aa / 54K
346 aa / 39K
502 aa / 57K464 aa / 53K
372 aa / 43K
305 aa / 35K
303 aa / 34K326 aa / 37K
360 aa / 41K
292 aa / 33K
297 aa / 34K298 aa / 34K
528 aa / 60K
xxxxlpxSPxkkxxx
xxxxxPlSPxrxxxx
xxxsxplSPxkxxxx
xxpxxxxSPxvxtlw
xPxsgsxSPxyxxxx
xxrxrxxSxxxxxxx
xxpsRpgSPxxxxxx
xxgsRpxSPxpxxpp
xxxqxppSPxxxxxa
xxxSpppSPppSpxx
xxpxpPlSPtppxxx
xxxsalxSPxaxxxx
xxxxxpxSpxxxxxx
xxxxxPlSPxxpxxx
xxxxrpxSpxxxxxx
RSRSRSRSRSRSRSR
0 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500
CDK1 P06493CDK2 P24941CDK3 Q00526
CDK10 Q15131
CDK4 P11802CDK6 Q00534
CDK5 Q00535
CDK7 P50613
CDK11 Q9BWU1CDK8 P49336
CDK9 P50750
CHED Q14004CRK7 Q9NYV4
PITSLRE Q9UQ88
PCTAIRE1 Q00536PCTAIRE2 Q00537PCTAIRE3 Q07002PFTAIRE1 O94921PFTAIRE2 Q96Q40
CCRK Q8IZL9
CDKL1 Q00532CDKL2 Q92772CDKL3 Q8IVW4CDKL4 Q5MAI5CDKL5 O76039
CLK1 P49759CLK2 P49760CLK3 P49761CLK4 Q9HAZ1
DYRK1A Q13627DYRK1B Q9Y463
DYRK2 Q92630DYRK3 O43781DYRK4 Q9NR20
HIPK1 Q86Z02HIPK2 Q9H2X6HIPK3 Q9H422HIPK4 Q8NE63
PRP4 Q13523
GSK3α P49840GSK3β P49841
ERK1 P27361ERK2 P28482ERK3 Q16659ERK4 P31152ERK5 Q13164
ERK7 Q8TD08
JNK1 P45983JNK2 P45984JNK3 P53779
NLK Q9UBE8
p38α Q16539p38β Q15759p38δ O15264p38γ P53778
ICK Q9UPZ9MAK P20794MOK Q9UQ07
MSSK1 Q9UPE1SRPK1 Q96SB4SRPK2 P78362
S
S
S
RGC GROUP
RGC FamilyS
S
S
S
S
T
T
T
T
T
CA
A
A
A
C
C
C
C
A
S
S
0 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500
1061 aa / 119K1047 aa / 117K
1103 aa / 120K1108 aa / 125K
1073 aa / 123K
ANPα P16066ANPβ P20594CYGD Q02846CYGF P51841HSER P25092
S
S
S
S
S
S
S
S
T
S
S
S
S
337 aa / 39K337 aa / 39K
415 aa / 47K416 aa / 47K422 aa / 48.5K415 aa / 47K
447 aa / 51K
1414 aa / 152K
1244 aa / 137K
396 aa / 45.5K508 aa / 58K
474 aa / 53K
gxxxdddSxxgxxxx
xxxxxxtTxxxxxxx
CK1 GROUP
CK1 Family
TTBK Family
VRK Family
CK1α1 P48729CK1α2 Q8N752CK1δ P48730CK1ε P49674CK1γ1 Q9HCP0CK1γ2 P78368CK1γ3 Q9Y6M4
TTBK1 Q5TCY1TTBK2 Q6IQ55
VRK1 Q99986VRK2 Q86Y07VRK3 Q8IV63
0 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500
C
S
S
S
P
P
S
S
S
S
S
SP
P
S
S
P
PS
C
C
P
P
P
P
P
S P
C
C
S
S
P
P
S
S
S
P
P
P
C
C
C
P
P
PS
S
S
S
S
S
P
P
S
S
S
S
S
S
S
S
S
P
AGC GROUP
AKT Family
DMPK Family, GEK Subfamily
DMPK Family, ROCK Subfamily
DMPK Family, Unique Subfamily
GRK Family, BARK Subfamily
GRK Family, GRK Subfamily
MAST Family
NDR Family
PKA Family
PDK1 Family
PKC Family, Alpha Subfamily
PKC Family, Delta Subfamily
PKC Family, Eta Subfamily
PKC Family, Iota Subfamily
PKG Family
PKN Family
RSK Family, MSK Subfamily
RSK Family, p70 Subfamily
RSK Family, RSK Subfamily
RSK Family, Unique Subfamily
RSKL Family
SGK Family
YANK Family
AKT1 P31749AKT2 P31751AKT3 Q9Y243
DMPK1 Q09013DMPK2 Q6DT37MRCKα Q5VT25MRCKβ Q9Y5S2
ROCK1 Q13464ROCK2 O75116
CRIK O14578
BARK1 P25098BARK2 P35626
GPRK4 P32298GPRK5 P34947GPRK6 P43250GPRK7 Q8WTQ7RHOK Q15835
MAST1 Q9Y2H9MAST2 Q6P0Q8MAST3 O60307MAST4 O15021MASTL Q96GX5
LATS1 O95835LATS2 Q9NRM7NDR1 Q15208NDR2 Q9Y2H1
PKACα P17612PKACβ P22694PKACγ P22612PRKX P51817PRKY O43930
PDK1 O15530
PKCα P17252PKCβ P05771PKCγ P05129
PKCδ Q05655PKCΘ Q04759
PKCε Q02156PKCη P24723
PKCι P41743PKCζ Q05513
PKG1 Q13976PKG2 Q13237
PKN1 Q16512PKN2 Q16513PKN3 Q6P5Z2
MSK1 O75582MSK2 O75676
p70S6K P23443p70S6Kβ Q9UBS0
RSK1 Q15418RSK2 P51812RSK3 Q15349RSK4 Q9UK32
SgK494 Q96LW2
RSKL1 Q5VUN8RSKL2 Q9BSU9
SGK O00141SGK2 Q9HBY8SGK3 Q96BR1
YANK1 Q8WU08YANK2 Q8IY14YANK3 Q86UX6
480 aa / 56K481 aa / 56K479 aa / 56K
P
P
P
P
P
P
P
P
P
C
C
C
C
C
C C
C
C C
C C
C
C
C
P
P
P
P
P
P
P
P
P
R
R
R
R
R
R
R
S
S
S
S
S
689 aa / 80K
P
P
P
P
S
1711 aa / 194K
S
S
S
P
P
P
P
P
P
S
C
C
C
C
C
C
C C
C C
C C
C C
C C
C C
C C
C
C
P
P
U
U
C
C C
C
P
P
H
H
H
H H
H H H
H H H
E
P
P
SP P
S PP
S PP
C
1388 aa / 161K
E
S P
S P
PS
PS
S P
S
P
C S
S
S
PSC
S
PS S
P S
PS
S SP
S SP
P
S P
S
S
639 aa / 70.4K1551 aa / 172K
1354 aa /
688 aa / 80K
578 aa / 67K590 aa / 68K
576 aa / 66K553 aa / 62K563 aa / 63.5K
1570 aa / 171K
C
M
M S
P C
P
P 1719 aa / 196K
158K
2027 aa / 231K
1798 aa / 196K
1309 aa / 143K
2626 aa / 284K879 aa / 97K
1130 aa / 127K1088 aa / 120K
465 aa / 54K464 aa / 54K
350 aa / 40.5K350 aa / 40.5K350 aa / 40K
277 aa / 32K358 aa / 41K
556 aa / 63K
671 aa / 77K670 aa / 77K697 aa / 78K
676 aa / 77.5K706 aa / 82K
737 aa / 84K682 aa / 77K
587 aa / 67K592 aa / 68K
670 aa / 77K762 aa / 87K
942 aa / 104K984 aa / 112K
889 aa / 99K
802 aa / 90K772 aa / 86K
525 aa / 59K482 aa / 53.5K
735 aa / 83K740 aa / 84K733 aa / 83K745 aa / 84K
274 aa / 31K
1054 aa /117K541 aa / 59K
431 aa / 49K427 aa / 48K
496 aa / 57K
396 aa / 46K414 aa / 48K
486 aa / 55K
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0 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500
Scientific Content: Dr. Steven Pelech, University of British Columbia and Kinexus Bioinformatics Corporation, Vancouver, B.C., Canada; Dr. Janet L. Smith and Dr. Yanji Xu, Boston Biomedical Research Institute, Watertown, Mass., U.S.A Graphic Design: Dr. Steven Pelech; Cameron Bowyer, www.carbondigital.ca.
Reversible phosphorylation is the premier means of post-translational modification of proteins for their regulation in eukaryotic cells. Within the phosphoproteome, there appears to be over 500,000 phosphorylation sites distributed amongst most of the 23,000 proteins encoded by the human genome. This is catalyzed by at least 478 conventional protein kinases and 38 atypical protein kinases, which constitute the human kineome. The conventional protein kinases include 388 protein-serine/threonine kinases and 90 protein-tyrosine kinases. There are 58 receptor-tyrosine kinases and 32 non-receptor tyrosine kinases in humans. Due to alternative splicing, there are at least 140 additional splice variants of human protein kinases besides those shown in this poster. Defective cell signalling through malfunctioning protein kinases have been linked with over 400 human diseases, including cancer, diabetes, Alzheimer disease and other immunological and neurological disorders. More than a third of total R&D expenditures in large pharma is currently directed towards the exploitation of less than 20% of these protein kinases as drug targets. Over 150 compounds that are selective kinase inhibitors are presently undergoing human clinical trials, and at least 500 additional compounds are in pre-clinical animal trials. Ultimately, mapping the complex interactions between protein kinases, their effectors and substrates will yield a rich harvest of reliable biomarkers for patient diagnosis and suitable targets for therapeutic intervention to pave the way for personalized medicine delivery.
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