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Methods in Molecular Biophysics ( PDFDrive.com )dosequis.colorado.edu/Courses/Biofizz/Sections/Section27_794-837.… · Methods in Molecular Biophysics ( PDFDrive.com ).pdf Author:
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1(r) 4(r)
5(r)
3(r)2(r)
+ . . .
+ . . .
| |
| | | |
(a)
(b)
-
m = 2
x = a
x = a X = 3a0
m = 1
-
Sphere (1:1:1)
Guinier Gaussian
Ellipsoid (1:1:5)Log
G
0 2 4 6 8 10
0
2
4
6
8
G = 85 •
-
0 1 20
0.5
1.0
0
0.5
1.0
0 1 2
( )( )
( = 0) ( = 1)
x=1
< > x= 4x 1/4< >
1/4
0 1 20
0.5
1.0
0
0.5
1.0
0 1 2
( )( )1/
( = 0) ( = 1)x
=1< > x
= 4x 1/4 = 2x 1/8< >
( = 2)x< >
1/4
-
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8
4
5
6
7
8
( )
( )
0
1
2
3
0 10 20 30 40 50 60
(a)
0 10
(b)
20 30 40 50 60 70 80 90 0 10 20 30 40 50 60 70 80 90
0 10 20 30 40 50 60 70 80 900 10 20 30 40 50 60 70 80 90
( )
0
1
2
3( )
0
1
2
3
( )
0
1
2
( )
0
1
2
-
(a) (b) (c)
-
(a)
(b)
(c) (f)
(e)
(d)
-
0 5 10 15 20 25 300
1
2
3
4( )
12
12
( ) = 2 + 2sin 12
12
(a)
0 5 10 15 20 25 300
1
2
3
4( )
12
12
(b)
-
1
( )(a)
0 5 10 15 20
0.2
0.2
0.4
0.6
0.8
1.0( )(b)
0
(c)
5 10 15 20
0.2
0.4
0.6
0.8
1.0
2( )
-
t
c
(a)
(b)
-
Scatte
rin
g len
gth d
ensi
ty 10
10 c
m2
-
(a)
2 = 2 2
C1C2
1, G12, G2
L12
(b)
-
50 S
30 S
11,000
9000
7000
6000
7000
5000
3000
65 2 •
RNA
G RNA
66 2 •
81 3 •G,prot
102 3 •G,prot
0 0.2 0.4 0.6 0.8 1.0
Scatter ing fr action prot
2 G (
•2)
-
Scatter ing length density , 1010 cm 2
(1)
(2)
(3)
(4)
SAXS SANS in H 2O SANS in D 2O
0
2
4
6
8
10
12
2
(a)
0.0
(b)
1.0 2.0 3.0
(nm 1)
2
1
ln
(1)
(2)
(3)
0
( )
F( )+
=
-
20
10
0
10
0 50 100
0/
, a
rbitr
ary
un
its
Vol fr action D 2O
(a)
2
(• 2)
8000 90
80
70
60
50
4030
1 0
(b)
1
6000
4000
2000
(•)
1/
-
0
(a)
100 200
(•)
0.5
0
1( )
ATP
ThÐA TP
ThÐA TP*
Th
ThÐADP
Pi
(b)
ThÐADPÐPi
ADP
Rearrangementat ph ysiologicaltemper atures
Fast h ydrolysisat ph ysiologicaltemper atures
Slo w h ydrolysisat lo wer
temper atures
-
(a)
0.000
(b)
0.002 0.004 0.006 0.008 0.0102 (• 2)
4.2
4.0
3.8
3.6
3.4
3.2
3.0
2.8
2.6ln
[(
)]
Guinier Plots f or bR in d63 -DMPC & d 67 -DMPC
0
(c)
20 40 60 80 10010
5
0
5
10
15
20
%D 2O
[(0
)/]1
/2
