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Millipore recognizes the need to better understand the role that bone metabolism biomarkers like osteocalcin play both in preserving normal bone structure and in the development of disease. This product guide includes a list of our available MILLIPLEX MAP Bone Metabolism panels, based on the Luminex xMAP platform. MILLIPLEX MAP enables you to investigate the modulation and expression of dozens of analytes simultaneously, giving you the advantage of speed and sensitivity, and dramatically improving productivity.
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Data SheetProduct Selection Guide
MILLIPLEX™ MAP and Bone MetabolismLuminex® xMAP® Technology
RAT
OPG
PTH
ACTH
RANKL
HUMAN
RAT
MOUSE
Your Only Source for Bone Metabolism Multiplex Immunoassays BONE METABOLISM
MILLIPLEX MAP Bone MetabolismUnderstanding the delicate metabolic balance between bone deposition and resorption and identifying its biomarkers is essential for determining how to maintain balance and treat disruption of this equilibrium through disease.
Millipore recognizes the need to better understand the
role that bone metabolism biomarkers like osteocalcin
play both in preserving normal bone structure and in the
development of disease. This product guide includes a list of
our available MILLIPLEX MAP Bone Metabolism panels, based
on the Luminex xMAP platform. MILLIPLEX MAP enables you
to investigate the modulation and expression of dozens of
analytes simultaneously, giving you the advantage of speed and
sensitivity, and dramatically improving productivity.
Bone Metabolism Research plays a significant role in achieving a deeper understanding of disease states such as:
•Osteoarthritis
•RheumatoidArthritis
•ChronicKidneyDisease
•BoneMetastasis
Rat Bone Metabolism Panels •BonePanel1
•BonePanel2
•BonePanel3
•OPGSinglePlex
•OsteocalcinSinglePlex
•RANKLSinglePlex
Available Panels:
Bone Turnover Cycle
Bone Remodeling Unit
Osteoclasts
ACTIVATION
REVERSAL
Bone Resorption 10-20 d
Osteoclast Apoptosis
FORMULATION
Collagen Formulation/mineralization
Osteoblasts
Bone Formulation 3-6 Months
RESTING PHASE
HUMAN RATMOUSE
Human Bone Metabolism Panels •BonePanel1A(serum/plasma)•BonePanel1B(tissueculture)•RANKLSinglePlex
Mouse Bone Metabolism Panels •BonePanel1A(serum/plasma)•BonePanel2A(serum/plasma)•BonePanel1B(tissueculture)•BonePanel2B(tissueculture)•OPGSinglePlex•OsteocalcinSinglePlex•RANKLSinglePlex
MILLIPLEX MAP Bone Metabolism Multiplex PanelsBone metabolism is the dynamic process of ongoing bone deposition and
resorption, controlled by osteoblasts, osteocytes, and osteoclasts. While
osteoblasts and osteocytes (osteoblasts surrounded by matrix) are responsible
for bone deposition, osteoclasts are responsible for bone resorption. Both are
required to maintain bone structure, as well as an adequate supply of calcium.
To maintain this metabolic balance these cells rely on complex signaling pathways
involving hormones and cytokines to achieve the appropriate rates of growth
and differentiation. The disruption of bone metabolism results in such disease
as osteoporosis, osteoarthritis, rheumatoid arthritis, chronic kidney disease and
bone metastases.
Three of these proteins are osteocalcin, osteoprotegerin (OPG) and RANKL. • Osteocalcinissecretedbyosteoblastswithincreasedlevelsfoundinbonediseases
characterizedbyincreasedboneturnover.
- Osteocalcinmeasurementhasbeenusedtoidentifywomenatrisk
forosteoporosis.
• OPGcaninhibittumorbonemetastasisandbonelossbyinhibitingboneresorption.
• RANKLactivatesosteoclastsandhasbeenimplicatedindegenerativebonediseases
suchasrheumatoidarthritisandosteomyelitis.
- ThebalancebetweenOPGandRANKLregulatesosteoclastdifferentiation,
activationandsurvival.
AVAILABLE ANALYTESHuman Panel 1AHuman Panel 1B
Human Single PlexMouse Panel 1AMouse Panel 2AMouse Panel 1BMouse Panel 2B
Mouse Single PlexRat Panel 1Rat Panel 2Rat Panel 3
Rat Single Plex
Adi
pone
ctin
Inte
rleu
kin-
1β
(IL-1β)
Inte
rleu
kin-
6 (IL
-6)
Insu
lin
Lept
in
Ost
eoca
lcin
Ost
eopo
ntin
Adr
enoc
orti
cotr
opic
H
orm
one
(ACTH
)
Ost
eopr
oteg
erin
(O
PG)
Para
thyr
oid
H
orm
one
(PTH
)
RA
NKL*
Tum
or N
ecro
sis
Fa
ctor
α (TN
Fα)
*RANKL- Receptor Activator of Nuclear Factor Kappa B Ligand
Human Bone Metabolism Panels
100,000
Human Bone Panel 1AStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
1,00
0,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
Human RANKL Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
SerumMatrix
100,000
Human Bone Panel 1BStandard Curves in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
1,00
0,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
1
OPG
PTH
Leptin
ACTH
Osteopontin
Insulin
Osteocalcin
IL-1β
Adiponectin
IL-6
TNFα
100,000
Human Bone Panel 1AStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
1,00
0,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
Human RANKL Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
SerumMatrix
100,000
Human Bone Panel 1BStandard Curves in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
1,00
0,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
1
OPG
PTH
Leptin
ACTH
Osteopontin
Insulin
Osteocalcin
IL-1β
Adiponectin
IL-6
TNFα
100,000
Human Bone Panel 1AStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
1,00
0,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
Human RANKL Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
SerumMatrix
100,000
Human Bone Panel 1BStandard Curves in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
1,00
0,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
1
OPG
PTH
Leptin
ACTH
Osteopontin
Insulin
Osteocalcin
IL-1β
Adiponectin
IL-6
TNFα
100,000
Human Bone Panel 1AStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
1,00
0,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
Human RANKL Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
SerumMatrix
100,000
Human Bone Panel 1BStandard Curves in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
1,00
0,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
1
OPG
PTH
Leptin
ACTH
Osteopontin
Insulin
Osteocalcin
IL-1β
Adiponectin
IL-6
TNFα
SpecificityCross-reactivitybetweentheantibodiesandanyofthe
otheranalytesinthispanelisnon-detectableornegligible.
Assay Conditions
Bone Panel 1A: Serum/plasma samples
• Thiskitmaybeusedfortheanalysisofallorany
combinationoftheanalytesinthispanelinserumor
plasma.
• A1:4dilutionisrequiredforbothserumandplasma
samples.
• Thisisanovernightassayrequiring25µLsamplesize.
Bone Panel 1B: Cell/tissue culture samples
• Thiskitmaybeusedfortheanalysisofallorany
combinationoftheanalytesinthispanelincellor
tissueculturesamples.
• Thisisanovernightassayrequiring25µLsamplesize.
RANKL Single Plex
• Thiskitmaybeusedfortheanalysisofallorany
combinationoftheanalytesinthispanelinserum,
plasma,otherbodyfluids,andcell/tissueextractor
culturesamples.
• A1:4dilutionisrequiredforbothserumandplasma
samples.
• Thisisanovernightassayrequiring25µLsamplesize.
PERfORMANCE SPECifiCATiONS
HUMAN
HUMAN BONE METABOLiSM PANEL – PERfORMANCE SPECifiCATiONS
AnalyteStandard
Curve Range (pg/mL)
Sensitivity* Precision Accuracy (%)
Panel 1A Panel 1B Panel 1A Panel 1B Panel 1A Panel 1B
Adiponectin 61.0–250,000 – 22.2 – 3.3/10.2 – 96.9
ACTH 2.4–10,000 2.1 1.3 4.9/5.6 3.4/7.8 89.2 96.6
Insulin 61.0–250,000 46.3 38.5 3.5/6.3 2.7/5.5 95.2 100.0
IL-1b 0.5–2,000 – 0.13 – 3.5/7.3 – 93.3
IL-6 0.5–2,000 – 0.09 – 3.9/6.1 – 94.5
Leptin 61.0–250,000 119.7 18.8 4.8/6.2 3.7/4.7 136.3 94.0
Osteocalcin 24.4–100,000 39.3 39.8 5.6/10.7 3.7/6.7 98.4 101.2
Osteopontin 61.0–250,000 67.8 32.8 5.4/5.8 4.0/5.6 79.7 103.7
OPG 2.4–10,000 1.4 0.9 6.5/6.3 3.2/4.5 96.4 102.5
PTH 2.4–10,000 0.3 0.3 7.0/9.6 5.2/9.8 98.1 98.1
TNFa 0.5–2,000 – 0.06 – 4.8/5.9 – 101.2
Single Plex
RANKL 4.9–5,000 4.8 <5/<6 93.2
*MinimumDetectableConcentration
100,000
Mouse Bone Panel 1AStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL) Concentration (pg/mL)
100,000
Mouse Bone Panel 1BStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse Bone Panel 2BStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
100,000
Mouse Bone Panel 2AStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
Mouse Osteocalcin Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse OPG (Osteoprotegrin) Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse RANKL Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Leptin
ACTH
Insulin
IL-6
TNFα
RANKL
OPG
Leptin
ACTH
Insulin
Osteocalcin
IL-6
TNFα
RANKL
100,000
Mouse Bone Panel 1AStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL) Concentration (pg/mL)
100,000
Mouse Bone Panel 1BStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse Bone Panel 2BStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
100,000
Mouse Bone Panel 2AStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
Mouse Osteocalcin Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse OPG (Osteoprotegrin) Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse RANKL Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Leptin
ACTH
Insulin
IL-6
TNFα
RANKL
OPG
Leptin
ACTH
Insulin
Osteocalcin
IL-6
TNFα
RANKL
100,000
Mouse Bone Panel 1AStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL) Concentration (pg/mL)
100,000
Mouse Bone Panel 1BStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse Bone Panel 2BStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
100,000
Mouse Bone Panel 2AStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
Mouse Osteocalcin Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse OPG (Osteoprotegrin) Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse RANKL Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Leptin
ACTH
Insulin
IL-6
TNFα
RANKL
OPG
Leptin
ACTH
Insulin
Osteocalcin
IL-6
TNFα
RANKL
Mouse Bone Metabolism Panels
SpecificityCross-reactivitybetweentheantibodiesandanyofthe
otheranalytesinthispanelisnon-detectableornegligible.
Assay Conditions
Bone Panels 1A and 2A: Serum/plasma samples
• Thesekitsmaybeusedfortheanalysisofallorany
combinationoftheanalytesineachpanelinserum
orplasma.
• Theseareovernightassaysrequiring25µLsamplesize.
Bone Panels 1B and 2B: Cell/tissue culture
• Thesekitsmaybeusedfortheanalysisofallorany
combinationoftheanalytesineachpanelincellor
tissueculturesamples.
• Theseareovernightassaysrequiring25µLsamplesize.
RANKL, Osteocalcin, and OPG Single Plexes
• Thesekitsmaybeusedfortheanalysisoftheanalytes
ineachpanelinserum,plasma,otherbodyfluids,and
cell/tissueextractorculturesamples.
• A1:20dilutionisrequiredforbothserumandplasma
sampleswhenusingtheOsteocalcinSinglePlex.
• Theseareovernightassaysrequiring25µLsamplesize.
PERfORMANCE SPECifiCATiONS
MOUSEHUMAN
MOUSE BONE METABOLiSM PANEL – PERfORMANCE SPECifiCATiONS
AnalyteStandard
Curve Range (pg/mL)
Sensitivity (pg/mL) Precision intra-/inter (%CV) Accuracy (%)
Panel 1A
Panel 2B
Panel 1B
Panel 2B
Panel 1A
Panel 2B
Panel 1B
Panel 2B
Panel 1A
Panel 2B
Panel 1B
Panel 2B
ACTH 2.4–10,000 1.8 1.7 1.4 1.34.0/
8.9
2.9/
5.5
2.9/
7.5
2.9/
7.5117 115 92 85
Insulin 24.4–25,000 18.6 16.2 17.4 14.42.2/
8.3
2.7/
4.0
2.2/
7.2
2.7/
4.892 92 101 91
IL-6 2.4–10,000 0.6 0.6 0.6 0.63.7/
7.1
3.7/
4.4
3.7/
8.6
3.7/
3.486 89 87 90
Leptin 9.8–40,000 3.0 4.5 3.2 4.43.7/
7.2
3.2/
3.7
3.7/
9.8
3.2/
6.587 93 93 93
Osteocalcin 9.8–40,000 – – 8.5 4.7 – –2.3/
8.3
3.2/
6.5– – 99 97
OPG 9.8–40,000 2.3 – 2.4 –3.1/
6.4–
3.1/
8.7– 95 – 90 –
RANKL 9.8–40,000 – 3.3 – 2.7 –3.1/
9.0–
3.1/
11.0– 119 – 78
TNFa 2.4–10,000 1.0 0.8 0.8 0.62.5/
6.0
2.5/
4.4
2.5/
6.2
2.5/
9.3112 133 92 93
Single Plex
Osteocalcin 9.8–40,000 7.0 2.3/7.3 99
OPG 9.8–40,000 2.3 3.1/6.0 83
RANKL 9.8–40,000 3.0 3.1/7.1 113
100,000
Mouse Bone Panel 1AStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL) Concentration (pg/mL)
100,000
Mouse Bone Panel 1BStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse Bone Panel 2BStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
100,000
Mouse Bone Panel 2AStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
Mouse Osteocalcin Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse OPG (Osteoprotegrin) Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse RANKL Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Leptin
ACTH
Insulin
IL-6
TNFα
RANKL
OPG
Leptin
ACTH
Insulin
Osteocalcin
IL-6
TNFα
RANKL
100,000
Mouse Bone Panel 1AStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL) Concentration (pg/mL)
100,000
Mouse Bone Panel 1BStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse Bone Panel 2BStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
100,000
Mouse Bone Panel 2AStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
Mouse Osteocalcin Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse OPG (Osteoprotegrin) Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse RANKL Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Leptin
ACTH
Insulin
IL-6
TNFα
RANKL
OPG
Leptin
ACTH
Insulin
Osteocalcin
IL-6
TNFα
RANKL
100,000
Mouse Bone Panel 1AStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL) Concentration (pg/mL)
100,000
Mouse Bone Panel 1BStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse Bone Panel 2BStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
100,000
Mouse Bone Panel 2AStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
Mouse Osteocalcin Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse OPG (Osteoprotegrin) Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse RANKL Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Leptin
ACTH
Insulin
IL-6
TNFα
RANKL
OPG
Leptin
ACTH
Insulin
Osteocalcin
IL-6
TNFα
RANKL
100,000
Mouse Bone Panel 1AStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL) Concentration (pg/mL)
100,000
Mouse Bone Panel 1BStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse Bone Panel 2BStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
100,000
Mouse Bone Panel 2AStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
Mouse Osteocalcin Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse OPG (Osteoprotegrin) Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse RANKL Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Leptin
ACTH
Insulin
IL-6
TNFα
RANKL
OPG
Leptin
ACTH
Insulin
Osteocalcin
IL-6
TNFα
RANKL
100,000
Mouse Bone Panel 1AStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL) Concentration (pg/mL)
100,000
Mouse Bone Panel 1BStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse Bone Panel 2BStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
100,000
Mouse Bone Panel 2AStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
Mouse Osteocalcin Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse OPG (Osteoprotegrin) Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse RANKL Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Leptin
ACTH
Insulin
IL-6
TNFα
RANKL
OPG
Leptin
ACTH
Insulin
Osteocalcin
IL-6
TNFα
RANKL
100,000
Mouse Bone Panel 1AStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL) Concentration (pg/mL)
100,000
Mouse Bone Panel 1BStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse Bone Panel 2BStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
100,000
Mouse Bone Panel 2AStandard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
Mouse Osteocalcin Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse OPG (Osteoprotegrin) Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Mouse RANKL Single PlexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Leptin
ACTH
Insulin
IL-6
TNFα
RANKL
OPG
Leptin
ACTH
Insulin
Osteocalcin
IL-6
TNFα
RANKL
MOUSEMOUSE
Rat Bone Metabolism Panels
SpecificityCross-reactivitybetweentheantibodiesandanyofthe
otheranalytesinthispanelisnon-detectableornegligible.
Assay Conditions
Bone Panels 1, 2, and 3
• Thesekitsmaybeusedfortheanalysisofallorany
combinationoftheanalytesineachpanelinserumor
plasma,cellortissueculturesamples.
• Theseareovernightassaysrequiring25µLsamplesize.
RANKL, Osteocalcin, and OPG Single Plexes
• Thesekitsmaybeusedfortheanalysisoftheanalytes
ineachpanelinserum,plasma,otherbodyfluids,and
cell/tissueextractorculturesamples.
• A1:50dilutionisrequiredforbothserumandplasma
sampleswhenusingtheOsteocalcinSinglePlex.
• Theseareovernightassaysrequiring25µLsamplesize.
PERfORMANCE SPECifiCATiONS
100,000
Rat Bone Panel 1Standard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL) Concentration (pg/mL)
100,000
Rat Bone Panel 3Standard Curves in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
100,000
Rat Bone Panel 2Standard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
Rat Osteocalcin Single-plexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Rat OPG (Osteoprotegrin) Single-plexStandard Curve in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Rat RANKL Single-plexStandard Curve in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
OPG
PTH
Leptin
Osteopontin
Insulin
Osteocalcin
RANKL
ACTH
100,000
Rat Bone Panel 1Standard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL) Concentration (pg/mL)
100,000
Rat Bone Panel 3Standard Curves in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
100,000
Rat Bone Panel 2Standard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
Rat Osteocalcin Single-plexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Rat OPG (Osteoprotegrin) Single-plexStandard Curve in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Rat RANKL Single-plexStandard Curve in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
OPG
PTH
Leptin
Osteopontin
Insulin
Osteocalcin
RANKL
ACTH
100,000
Rat Bone Panel 1Standard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL) Concentration (pg/mL)
100,000
Rat Bone Panel 3Standard Curves in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
100,000
Rat Bone Panel 2Standard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
Rat Osteocalcin Single-plexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Rat OPG (Osteoprotegrin) Single-plexStandard Curve in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Rat RANKL Single-plexStandard Curve in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
OPG
PTH
Leptin
Osteopontin
Insulin
Osteocalcin
RANKL
ACTH
100,000
Rat Bone Panel 1Standard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL) Concentration (pg/mL)
100,000
Rat Bone Panel 3Standard Curves in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
100,000
Rat Bone Panel 2Standard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
Rat Osteocalcin Single-plexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Rat OPG (Osteoprotegrin) Single-plexStandard Curve in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Rat RANKL Single-plexStandard Curve in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
OPG
PTH
Leptin
Osteopontin
Insulin
Osteocalcin
RANKL
ACTH
100,000
Rat Bone Panel 1Standard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL) Concentration (pg/mL)
100,000
Rat Bone Panel 3Standard Curves in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
100,000
Rat Bone Panel 2Standard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
Rat Osteocalcin Single-plexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Rat OPG (Osteoprotegrin) Single-plexStandard Curve in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Rat RANKL Single-plexStandard Curve in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
OPG
PTH
Leptin
Osteopontin
Insulin
Osteocalcin
RANKL
ACTH
100,000
Rat Bone Panel 1Standard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL) Concentration (pg/mL)
100,000
Rat Bone Panel 3Standard Curves in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
100,000
Rat Bone Panel 2Standard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
Rat Osteocalcin Single-plexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Rat OPG (Osteoprotegrin) Single-plexStandard Curve in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Rat RANKL Single-plexStandard Curve in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
OPG
PTH
Leptin
Osteopontin
Insulin
Osteocalcin
RANKL
ACTH
100,000
Rat Bone Panel 1Standard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL) Concentration (pg/mL)
100,000
Rat Bone Panel 3Standard Curves in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0M
edia
n Fl
uore
scen
ceIn
tens
ity
(MFI
)
100,000
Rat Bone Panel 2Standard Curves in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
Rat Osteocalcin Single-plexStandard Curve in Assay Buffer
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Rat OPG (Osteoprotegrin) Single-plexStandard Curve in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
Rat RANKL Single-plexStandard Curve in Serum Matrix
10,000
1,000
100
10
10 10 10
0
1,00
0
10,000
100,00
0
Med
ian
Fluo
resc
ence
Inte
nsit
y (M
FI)
Concentration (pg/mL)
100,000
OPG
PTH
Leptin
Osteopontin
Insulin
Osteocalcin
RANKL
ACTH
RATRAT
RAT BONE METABOLiSM PANEL – PERfORMANCE SPECifiCATiONS REfERENCES
AnalyteStandard
Curve Range (pg/mL)
Sensitivity (pg/mL) Precision intra-/inter (%CV) Accuracy (%)
Panel 1 Panel 2 Panel 3 Panel 1 Panel 2 Panel 3 Panel 1 Panel 2 Panel 3
ACTH 2.4–10,000 1.8 1.7 – 4.0/8.9 2.9/5.5 – 99 102 –
Insulin 24.4–100,000 18.6 16.2 – 2.2/8.3 2.7/4.0 – 76 76 –
Leptin 9.8–40,000 3.0 4.5 – 3.7/7.2 3.2/3.7 – 78 88 –
Osteocalcin 5–20,000 – – 1.3 – – 2.9/4.6 – – 100
Osteopontin 5–20,000 – – 0.8 – – 3.1/5.4 – – 85
OPG 9.8–40,000 2.3 – – 3.1/6.4 – – 79 – –
PTH 1–5,000 – – 0.3 – – 3.5/8.1 – – 78
RANKL 3.7–15,000 – 1.1 – – 3.1/9.0 – – 118 –
Single Plex
Osteocalcin 5–20,000 0.8 3.2/5.6 101
OPG 9.8–40,000 2.3 3.1/6.0 78
RANKL 3.7–3,750 1.0 3.1/7.1 105
StolinaM,DwyerD,OminskyM,CorbinT,VanG,BolonB,
SarosiI,McCabeJ,ZackD,KostenuikP.ContinuousRANKL
InhibitioninOsteoprotegerinTransgenicMiceandRats
SuppressesBoneResorptionwithoutImpairing
LymphorganogenesisorFunctionalImmuneResponses.
Journal of Immunology,179:7497-7505(2007)
WadaC,KataokaM,SetoH,HayashiN,KidoJ,ShinoharaY,
NagataT.High-turnoveosteoporosisisinducedby
cyclosporineAinrats.J None Miner Metab24:199-205
(2006)
KhoslaSandKleetrkoperM.Biochemicalmarkersofbone
turnover.In:FavusMJEd“PrimerontheMetabolicBone
DiseasesandDisordersofMineralMetabolism.American
Society for Bone and Mineral Research,Washington,DC”
166-172(2003)
DucyP,DesboisC,BoyceB,PineroG,StoryB,DunstanC,
SmithE,Bonadioj,GoldsteinS,GundbergC,BradleyA,
KarsentyG.Increasedboneformationinosteocalcin-
deficientmice. Nature382:448-452(1996)
FrolikCA,BryantHU,BlackEC,MageeDE,and
ChandrasekharS.Time-dependentchangesinbiochemical
bonemarkersandserumcholesterolinovariectomized
rats:effectsofraloxifeneHCl,tamoxifen,estrogen,and
alendronate.Bone18:621-627(1996)
NodaMandRodanGA.Transcriptionalregulationof
osteopontinproductioninratosteoblast-likecellsby
parathyroidhormone.J Cell Biol108:713-718(1989).
RAT
xMAP TECHNOLOGy PROCESS fLOw
With xMAP technology, you will benefit from
the ability to simultaneously quantitate up to
100 analytes in a single sample, using as little
as 25 µL or less.
• Speed/High-Throughput:Simultaneouslymeasure
theconcentrationofalargenumberofanalytes,
allowingyoutodomorefaster,gainingearlyand
comprehensivedatasocriticaltoyourwork.
• Accuracy:xMAPTechnologygeneratesreal-time
analysisandaccuratequantificationofbiological
interactions.
• Reproducibility:Thehigh-volumeproductionof
xMAPbeadsallowsassaystandardizationthat
solid-phasedflatarrayscannotprovide.
COMPARiSON Of THE xMAP & ELiSA TECHNOLOGiESSide-by-sidecomparisonofMultiplexingvs.ELISAs(Studyof10differentanalytesin80testsamples).
xMAP Technology ELISA
Number of plates required 1 10
Results per plate 800 80
Total sample used per panel 50µL 4mL
Internal controls possible Yes No
Dynamic Range 1-10,000pg/mL 10-2500pg/mL
Lower limit of detection ~1pg/mL ~1pg/mL
Luminex xMAP Technology
1 Luminexusesproprietarytechniquestointernallycolor-codemicrosphereswithtwofluorescentdyes.Throughpreciseconcentrationsofthesedyes,100distinctlycoloredbeadsetscanbecreated,eachofwhichiscoatedwithaspecificcaptureantibody.
3 Themicrospheresareallowedtopassrapidlythroughalaser,whichexcitestheinternaldyesmarkingthemicrosphereset.AsecondlaserexcitesPE,thefluorescentdyeonthereportermolecule.
2 Afterananalytefromatestsampleiscapturedbythebead,abiotinylateddetectionantibodyisintroduced.ThereactionmixtureisthenincubatedwithStreptavidinPEconjugate,thereportermolecule,tocompletethereactiononthesurfaceofeachmicrosphere.
Bead #12, IL-6Bead #29, IFN-γ
Bead #56, TNF-α
PRODUCTS • Higher Quality–Industry-leadingQC,QAandSixSigmaPrinciples• More Efficient–Analyticallyvalidatedpanelsforconsistencyandreproducibility• All-In-One Kit–Asinglekitwithasinglecatalognumber• More Flexible–Selectonlytheanalytesneededorchooseapre-mixedkit• Continuity of Knowledge Is the Key–Millipore“owns”everystepoftheprocess,
fromR&Dtomanufacturing
• More Choices–ThebroadestrangeofmultiplexanalytesfortheLuminexxMAPplatform
iNSTRUMENTS • Speed/High-Throughput–Simultaneouslymeasuretheconcentrationofalargenumber
ofdifferentanalytes,allowingyoutodomorefaster,gainingearlyandcomprehensive
datasocriticaltoyourwork.
• Accuracy–xMAPtechnologygeneratesreal-timeanalysisandaccuratequantificationof
thebiologicalinteractions.
• Reproducibility–High-volumeproductionofxMAPmicrospheresallowsassay
standardizationthatsolid-phasedflatarrayscannotprovide.
• Low Sample Volume–Withminimalhands-ontime,youcanscreenmorethan40
analytesinasinglesampleusingaslittleas25µLorless.
SOfTwARE • TheLuminex xPONENT® softwarehelpsyoumanageandtrackallofyourmultiplex
assaysbycombiningeaseofuseandflexibilitytoperformbothproteinandnucleicacid
testingononesystem.It’salsocompatiblewiththeupcomingmagneticbead(MagPlex™
beads)assays,andwillbemovingforwardwithcompatiblefutureinstrumentplatforms.
• NEw! MilliporenowoffersMILLIPLEX Analyst software,developedexclusivelyfor
MilliporebyVigeneTech.Thisdataanalysispackagehelpsyoudoyourbestworkwith
increasedspeedandefficiency.MILLIPLEXAnalystsoftwareprovidesawholenewlevel
ofdataanalysisbyallowingyoutoimportlargedatasetsandbenefitfromtheabilityto
calculateyourresultsinseconds.Theuser-friendlyplatesetupfeatureletsyouwork
withspeedandefficiencywhiletheuniquealgorithmsoneventhemostchallengingdata
setsgiveyoutheperformanceyouneedformultiplexinganalysis.
SERViCES • Millipore’s Assay Serviceshasbeenperformingimmunoassaysforover25years.• AssayservicesareavailableforRIAs,ELISAs,IRMAsandmultiplexassays,usingboth
Milliporekitsaswellasanycommerciallyavailableassays.
• Qualitycontrol,reproducibleresults,andconfidentialityareofparamountimportance
toourAssayServiceteam.
• Millipore’sRegulatory Compliant Laboratoryisuniquelypositionedtoprovide
bioanalyticalservicestosupportyourpreclinicalandclinicalstudies.
MILLIPLEX MAP: Everything You Need – All In One Place
MILLIPLEX MAP
MILLIPLEX Analyzer
MILLIPLEX Analyst
BioPharma Services
Foracompleteandup-to-datelistofMILLIPLEXMAPanalytes,visitwww.millipore.com/analytes.
TO PLACE AN ORDER Visitwww.millipore.com/milliplexorcall1-800-MILLIPORE.
fOR PRODUCT OR APPLiCATiON iNfORMATiON Visitwww.millipore.com/bmia,orcontactTechnicalService
(USTollFree)1-866-441-8400,1-636-441-8400,
TO REQUEST A SALES REP Email:[email protected].
Millipore,Upstate,Chemicon,Linco,andDuraporeareregisteredtrademarksofMilliporeCorporation.TheMmark,AdvancingLifeScienceTogether,andMILLIPLEXaretrademarksofMilliporeCorporation.Luminex,xPONENT,andxMAPareregisteredtrademarksofLuminexCorporation.MagPlexisatrademarkofLuminexCorporation.Lit.No.PB1073EN0008DD013PrintedinU.S.A.09/08DD-SBU-08-00908©2008MilliporeCorporation,Billerica,MA01821U.S.A.Allrightsreserved.
Description Quantity Catalogue No.
Human HumanBoneMetabolismPanel1A 96-wellplate HBN1A-51K
HumanBoneMetabolismPanel1B 96-wellplate HBN1B-51K
HumanRANKLSinglePlex 96-wellplate HBN51K1RANKL
Mouse MouseBoneMetabolismPanel1A 96-wellplate MBN1A-41K
MouseBoneMetabolismPanel2A 96-wellplate MBN2A-41K
MouseBoneMetabolismPanel1B 96-wellplate MBN1B-41K
MouseBoneMetabolismPanel2B 96-wellplate MBN2B-41K
MouseOPGSinglePlex 96-wellplate MBN-41K-1OPG
MouseOsteocalcinSinglePlex 96-wellplate MBN-41K-1OC
MouseRANKLSinglePlex 96-wellplate MBN-41K-1RANKL
Rat RatBoneMetabolismPanel1 96-wellplate RBN1-31K
RatBoneMetabolismPanel2 96-wellplate RBN2-31K
RatBoneMetabolismPanel3 96-wellplate RBN3-31K
RatOPGSinglePlex 96-wellplate RBN-31K-1OPG
RatOsteocalcinSinglePlex 96-wellplate RBN-31K-1OC
RatRANKLSinglePlex 96-wellplate RBN-31K-1RANKL
ORDERiNG iNfORMATiON
VigeneTech
www.millipore.com/offices
