MTech (Biotechnology) Curriuclum & Syllabi for AY2012-13

7/31/2019 MTech (Biotechnology) Curriuclum & Syllabi for AY2012-13

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M.Tech BiotechnologyApplicable to AY 2012-13 onwards

University Core

S. No Course Code Course Title L T P C1 ENG 601 Professional and Communication Skills

(or)Foreign Language

0

2

0

0

4

0

2

22 BIT 528 Statistics for Biological Applications 3 1 0 4

Total Credits 6

University Elective

S. No Course Code Course Title L T P C1 University Elective 3 0 0 3

Total Credits 3

Programme Core

S. No Course Code Course Title L T P C1 BIT 517 Cell Biology and Biochemistry 3 0 4 52 BIT 518 Biochemical Engineering 3 0 4 5

3 BIT 519 Molecular Biotechnology 3 0 4 54 BIT 521 Genetic Engineering 3 0 4 55 BIT 520 Bioprocess Technology 3 0 2 46 BIT 511 Immunotechnology 3 0 2 47 BIT 513 Genomics and Proteomics 3 0 2 48 BIT 537 Bioethics, Biosafety and Patents 3 0 0 39 BIT 699 Students Project - - - 20

Total Credits 55

Programme Elective

S. No Course Code Course Title L T P C1 BIT 522 Advanced Bioseparation Techniques 3 0 0 32 BIT 523 Food Process Technology 3 0 0 33 BIT 529 Metabolic Engineering and Metabolomics 3 0 0 34 BIT 514 Nano Biotechnology 3 0 0 35 BIT 527 Biobuisness Management 3 0 0 3


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6 BIT 530 Plant Biotechnology 3 0 0 37 BIT 524 Natural Products Technology 3 0 0 38 BIT 536 Medical Biotechnology 3 0 0 39 BIT 533 Environmental Biotechnology 3 0 0 310 BIT 502 Industrial Biotechnology 3 0 0 3

11 BIT 515 Protein Engineering 3 0 0 312 BIT 531 Animal Biotechnology 3 0 0 313 BIT 532 Pharmaceutical Biotechnology 3 0 0 314 BIT 525 In silico Drug Design 3 0 0 315 BIT 534 Systems Biology 3 0 0 316 BIT 538 Microbial Technology 3 0 0 317 BIT Marine and Aquatic Biotechnology 3 0 0 318 BIT Applied Bioinformatics 2 0 1 3

Total credits to be taken 9

Credit SummaryMinimum Qualifying Credits 73

Total Credits Offered 73University Core 6University Elective 3Programme Core Offered 55Programme Elective Needed 9


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UE for all M.Tech. programmesUniversity Elective

19 BIT Biodiversity and Conservation 3 0 0 3


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BIT 528 Statistics for Biological Applications 3 1 0 4

Course prerequisites: Knowledge on basic mathematics.

Objectives: This course is an introduction to statistical methods used in the public health, biological, andmedical sciences. Topics include descriptive statistics, performance characteristics of diagnostic tests,graphical methods, estimation, hypothesis testing, p-values, confidence intervals, correlation, linear regression, and clinical trials.Expected Outcomes: On learning the course students will be able to do statistical analysis for their research data.

ContentsIntroduction (9 hrs)Types of Biological data, frequency distribution, cumulative frequency distributions. Measure of centraltendencymean, median, mode, geometric mean & harmonic mean. Measure of dispersionrange,variance, standard deviation, coefficient of variation.

Line & curve fitting and Correlation & regression (9 hrs)Curve fitting by method of least squares, regression analysis, inferences based on least squares estimation,curvilinear (or non linear) regression, curve fitting by sum of exponentials, linear weightage least squareapproximation, correlation analysis, rank correlation.

Probability Theory (8 hrs)Review of set theory, mathematical and statistical probability. Laws of probability-addition andmultiplication laws, conditional probabilities. Probability distributionsnormal, binomial and Poissondistributions.

Theory of sampling and test of hypothesis (9 hrs)Populations, sample from populations, random sampling, parameters and statistical standard error.Testing for goodness of fit, Chi-square test, statistical significance, Large sample test, F- test, Student t-test, testing the significance of regression, comparisons of two slopes, hypothesis testing about correlationcoefficients.

Design of experiments and Analysis of variance (10 hrs)Design of experiments- Principles of experimentation- randomization, replication and local control.Analysis of variance- One way, Two way and Three way classification. Simple designs and analysis:completely randomised block design (CRD), randomised block design (RBD); latin square design (LSD)and factorial design.

Text Books:Jerold. H. Zair . 1999. Biostatistical Analysis, 4th eds. Prentice Hall Inc.Gupta S. C & V. K Kapoor. 2000. Fundamentals of Mathematical Statistics - Sultan Chand & Sons.Stew Selvin . 2005. Biostatistics, How it works. Pearson education.Reference Books:Ramana B. V. 2007. Engineering Mathematics, McGra-Hill companies.Sundar Rao, P. S. S. & Richard, J. 2003. An introduction to Biostatistics- A manual in health sciences,3rd eds. Prentice Hall of India Pvt Ltd.

Mode of Evaluation - Take home problems, assignments and Written Examinations.


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BIT 517 Cell Biology and Biochemistry 3 0 4 5

Course prerequisites: Biology and chemistry.

Objectives : To impart knowledge on prokaryotic and eukaryotic biochemistry.

Expected Outcome : Students will be able to apply the derived knowledge in comparing and contrasting biochemical pathways in living organisms which forms the foundation for any biological project work and research.

Principles of Biochemistry 10 hrsOrganelles and biomolecules of cells; Thermodynamics; Properties of water, pH and buffering action;Amino acids and peptides, protein structure, denaturation, folding and functions, enzymesmechanism of action and kinetics; Carbohydrates and glycoconjugates; Nucleic acid structure, chemistry and functions;Storage and structural lipids, lipids as signals, cofactors and elements; Bioenergetics, energy richcompounds and biological oxidation-reduction reactions.

Transport and signaling 8 hrsComposition, architecture and dynamics of biomembrane; ATP powered pumps, ion channels, andtransporters; Signal transduction pathways-G protein, Cytokine, tyrosine kinases, TGF, Hedgehog, Wntand Notch receptors; Signaling in microorganism and plants, Regulation of cell cycle by protein kinases;Apoptosis.

Microbial metabolism 9 hrsAnoxygenic and oxygenic photosynthesis, autotrophic CO 2 fixation; Hydrogen, sulphur, iron oxidation,nitrification and anammox; Aerobic, anaerobic respiration, its energetics; Nitrate reduction anddenitrification, sulphate reduction, acetogenesis, methanognesis, ferric iron, manganese, chlorate andorganic electron acceptors; Fermentation, glycolysis, syntrophy, hydrocarbon oxidation, and nitrogenfixation; Biosynthesis of sugars, polysaccharides, amino acids and nucleotides, lipids and fatty acid.

Plant metabolism 9 hrsPhotosynthesislight reactions and carbon reactions; Respiration and lipid metabolism; Assimilation of mineral nutrientsNitrogen and biological nitrogen fixation, sulphur, phosphate, cations and oxygen;Secondary metabolites and plant defense; Cell wall biogenesis; Stress biochemistry- biosynthesis of osmoprotectants; Biosynthesis of growth harmones.

Animal metabolism 9 hrsGlycolysis, gluconeogenesis and pentose phosphate pathway; Metabolism of glycogen; Citric acid cycle;Oxidative phosphorylation; Lipid catabolism and anabolism; Amino acid oxidation and urea production;Biosynthesis of amino acids and nucleotides; Hormones, tissue-specific metabolism and regulation.

Text and reference books1. Dave Nelson and Mike Cox. 2009. Lehninger Principles of Biochemistry. 5 th Edition. W.H.

Freeman Publishers. USA.2. Lincoln Taiz and Eduardo Zeiger. 2010. Plant Physiology, 5 th Edition. Sinauer Associates Inc.

USA.3. Harvey Lodish, Arnold Berk, Chris A. Kaiser, Monty Krieger, Matthew P. Scott, Anthony

Bretscher, Hidde Ploegh, and Paul Matsudaira. 2008. Molecular Cell Biology. 6th Edition. W.H.Freeman Publishers. USA.


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4. Michael Madigan, John Martinko, David Stahl, David Clark. 2011. Brock Biology of Microorganisms, 13 th Edition. Pearson Education. Inc. USA.

Mode of Evaluation: Written examination, assignments/projects and seminars.


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BIT 517L Cell Biology and Biochemistry

Contents

1. Statistical analyses and calculations in biochemistry, mass calculations & calibration of balances.

2. Solutions, concentrations and standard solutions & calibration of pipettes and burettes.

3. Acid-Base potentiometric, volumetric titrations and preparation of indicator solutions.

4. pKa estimations of acid/bases/buffers.

5. Preparation of buffers.

6. Fractionation of biopolymers.

7. Carbohydrate - qualitative (Benedict/Diesche) and quantitative analysis (DNS/Nelsons methods).

8. Estimation of cholesterol & free fatty acids.

9. Lipid profile analysis by TLC/GC.

10. Comparison of protein estimation methods: UV/ Dye binding/ Lowry/ Fluorimetric estimations.

11. Study of mitosis

12. Study of meiosis

Mode of Evaluation: Assignments, seminars, written examination


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BIT 518 Biochemical Engineering 3 0 4 5

Course prerequisites: Basic knowledge on biotechnology and chemical engineering.

Objectives: This course will help the students to

(i) Understand and apply the concepts of Material and Energy balances to bioprocesses(ii) Understand the reaction kinetics involved in bioprocesses and also model the kinetics(iii) Understand the concepts of heat transfer and apply them for heat transfer equipment design

Expected outcome : The students shall be able to take up research projects in biochemical engineeringand can make it their career.

Contents:

Material Balances (9 hrs)

Units and dimensions in Engineering calculations - Law of conservation of mass Material Balances withRecycle, By-pass and Purge systems Stoichiometry of growth and Product formation- elemental

balances theoretical oxygen demand- Estimation of maximum possible yield

Energy Balances (6 hrs)

General Energy Balance equations, Enthalpy, Entropy,Gibbs Free Energy thermodynamics of microbialgrowth

Heat transfer (9 hrs)

General equipment for heat transfer Mechanisms of heat transfer Conduction, convection Estimationof heat transfer coefficients

Mass Transfer (9 hrs)

Diffusion theory gas- liquid mass transfer Oxygen uptake in cell cultures Oxygen transfer infermenters Estimation of oxygen mass transfer coefficient factors affecting mass transfer rate

Reaction Kinetics (12 hrs)

Reaction rate Effect of temperature on reaction rate, Zero order kinetics,First- order kinetics,MIchealismenten kinetics Cell growth Kinetics Rates of growth, product formation and substrate uptakeKinetics of cell death, Models for cell growth, substrate and product inhibition structured models

Text Books

Pauline M.Doran, Bioprocess Engineering Principles,Academic Press,2005Shuler, M. and F. Kargi. 2002. Bioprocess Engineering. Prentice Hall (I) Ltd.,

Reference BooksReid and Rehm, 2003. Biotechnology Vol I to VIII. WILEY-VCH VerlagJ.E.Bailey and D.F.Ollis,Biochemical Engineering,Mc-GrawHill International Publications,1986


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Bhatt B.I., Vora S.M. Stoichiometry. 3rd Edition. Tata McGraw-Hill, 1977.Geankoplis C.J. Transport Processes And Unit Operations. Prentice Hall India.2002.

Mode of Evaluation: Written Examination. Assignments, Seminars


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BIT 518L Biochemical Engneering Lab

Application of chemical engineering concepts for bioprocesses

1. Mass transfer concepts during Ammonium sulfate precipitation2. Mass transfer concepts during Solvent extraction Liquid- Liquid, Aqueous Two phase.3. Estimation of Kinetic parameters for enzymatic reactions

Mode of Evaluation: Written examination, assignments and seminars.


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BIT 519 Molecular Biotechnology 3 0 4 5

Course prerequisites: Knowledge on cell biology.

Objectives: To provide knowledge on the fundamental aspects and techniques of molecular biology.

Expected Outcomes: Students will gain confidence in undertaking research work related to the field of molecular biology with the help of the knowledge gained from reading the course.

DNA (10 hrs)DNA genetic material, double helix structure, base pairing, semi conservative replication, cistron,multiple alleles, cis- acting sites and trans- acting molecules, interrupted gene : exon, intron organizationContent of the genome : genome organization, expression, organelle genome, clusters and repeats: geneduplication, pseudo genes, tandem repeats, cluster, satellites,

Transcription (9 hrs)Transcription, RNA Polymerases and their sub units, s-factor and promoter recognition, control of initiation, termination and anti-termination, eukaryotic transcription an overview, regulation of transcription, operon, Transcription activators : factors, repressors, activators, DNA binding domains -Znfinger proteins, steroid receptors, homeodomains, helix loop helix proteins, Leucine zippers.

RNA Splicing and Processing (9 hrs) Nuclear splicing, hnRNA, splice junctions, spliceosome, snRNA, scRNA, group-II introns and autosplicing, alternative splicing, yeast t-RNA splicing, Catalytic RNA : group-I intron self splicing bytransesterification, catalytic activities of ribozymes, RNAase P, viriods, RNA editing, protein splicing

Translation (9 hrs)Protein synthesis : Initiatiors, Elongation factors, termination codons, Using the Genetic code : codon-anticodon, Wobbling, modified bases of tRNA, charging of tRNAs, accuracy of translation, proof reading, suppressors, frame shifting, Protein localization passage, chaperones, protein folding,translocation signal sequences, anchor sequences, SRP, NLS, NES, Sec system, Ran, nuclear porecomplex, proteasome

Transposons and Signal transduction (9 hrs)Transposons - replicative and non-replicative mechanisms, common intermediates for transposons, Spm

elements, P elements, Retrovirus and retroposons : retrovirus life cycle and retroposons. Signaltransduction Ion channels, Neurotransmitters, G proteins, Protein kinase, Receptor kinase, Ras/MAPK,AMP, CREB, JAK-STAT, TGF signals.

Text BookBenjamin Lewin. 2004. Genes VIII. Pearson Prentice Hall

ReferenceGeoffrey M. Cooper. 2000. The Cell: A Molecular Approach. 2nd Edition. Sinauer Associates, Inc.James D. Watson et al., 2004. Molecular biology of the gene. Pearson education Inc.Lodish H. et. al., 2004. Molecular Cell Biology, 5th Edition, W.H. Freeman & Co.

Mode of Evaluation: Written examinations, assignments, seminars and quizzes


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BIT 519L Molecular Biotechnology

List of the experiments:

1. DNA isolation from bacteria and plant

2. DNA isolation from yeast and human blood

3. Plasmid DNA isolation

4. Native PAGE

5. SDS PAGE

6. Western blotting (Demonstration)

7. RNA extraction from prokaryotes and eukaryotes

8. Purification and quantification of DNA

Mode of Evaluation: Lab assignments, Viva voce and written exam


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BIT 521 Genetic Engineering 3 0 4 5

Course prerequisites: Molecular biology.

Objectives: To make students understand gene isolation and manipulation in-vitro , reintroduce into cell(in vivo ) and enable them to function in the foreign host. To make students understand the potentials of invitro DNA technology.Expected outcomes: Students will gain knowledge on gene manipulation and cloning. This course willhelp the students take up genetic engineering projects in the future.

Contents:Enzymes (8 hours)DNA modifying enzymes, polymerases, ligases, endo and exo nucleases, restriction enzymes and itstypes, adapters and linkers, homopolymer tailing, Labeling of DNA: Nick translation, Random priming,Radioactive and non-radioactive probes.

Vectors (9 hours)Plasmid and phage vectors, YAC, BAC, expression vectors, M13 vector, Plant, animal and yeast cloningvectors, vectors for chloroplasts and cosmid, phasmid and phagemid vectors. High capacity vectors, basicand advanced features of cloning vectors. Expression vectors: pMal; GST; Protein purification; His-tag;GST-tag; MBP-tag etc.; Intein-based vectors.

Gene Synthesis and Transformation (9 hours)Promoter, types, T7, pL, pho A, DNA sequencing, cDNA and genomic DNA library construction, PCR types and its application. Artificial gene synthesis, transformation methodologies in plants, animals andmicrobes.

Cloned Gene Expression (10 hours)Reporter genes, role and mechanism of GFP, CAT, luciferases and -galactosidases. Expressionsystems: Expression of foreign proteins in E. coli, Bacillus, Yeast, Insect and Mammalian cells. Genesilencing techniques: siRNA technology; Micro RNA; Construction of siRNA vectors; Principle andapplication of gene silencing; Gene knockouts and Gene Therapy; Creation of knock out mice.

Applications (9 hours)Transgenic plants, animals and microbes; Cloned organisms-plant and animal tissue engineering;Inducible gene expression in plants, animals and microbes.

Text / Reference Book: 1. Primrose, S.B. and Twyman, R.M., 2006. Principles of Gene Manipulation and Genomics. 7 th

Edition, Blackwell Publishing Co. UK 2. Dominic W.S. and Wong, 2006. The ABCs of Gene Cloning, 2 nd Edition, Springer International,

The Netherlands.3. Lisa Yount, 2008. Biotechnology and Genetic Engineering, 3 rd Edition, Facts on File Inc., USA.4. Russ Hodge, 2009. Genetic Engineering. 1 st Edition, Facts on File Inc., USA.5. Frank Kempken and Christian Jung, 2010. Genetic Modification of Plants, Springer International,

The Netherlands.6. Twyman, R.M. 2005. Gene Transfer to Animal Cells, Garland Science Publishers, USA.7. Sambrook et al., 2001. Molecular Cloning. A Laboratory Manual, 3rd edition, CSHL press.


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BIT 521L Genetic Engineering

List of the experiments:

1. RNA extraction, purification and quantification

2. cDNA synthesis

3. RT - PCR

4. Cloning

5. Competent cell preparation and transformation

6. Colony PCR

7. Plasmid DNA isolation and restriction digestion

8. RAPD (PCR) (Demonstration)

9. Real time PCR (Demonstration)

Text/Reference book

Sambrook and Russell, 2001. Molecular Cloning: A Laboratory Manual. 3rd edition, CSHL press.



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BIT 520 Bioprocess Technology 3 0 4 5

Course prerequisites: Microbiology

Objectives : This course will help the students to(i) have a thorough understanding of the various types of bioprocesess and the equipment used(ii) Understand and apply the concepts involved in design of media and sterilization methods(iii) Understand and apply the concepts involved in Scale- up

Expected outcomes: On successful completion of this course, students can gain confidence in bioreactor designing which will be useful for their future career.

Contents:Fermentation process (6 hours)Aerobic and Anaerobic fermentations - case studies from microbes, plant and animals

Bioreactors (9 hours)Classification, Accessories Parameters to be monitored and controlled in a bioreactor, Types of control Feed back and cascade Reactors for Animal and Plant cells

Media optimization and Sterilization (12 hours)Components of Fermentation media Media optimization methods Plackett Burman Design,Responsesurface methodology and Simple search method. Sterilization and its practical limits, Batch sterilisation,Continuous sterilisation, Filter Sterilization

Analysis of Bioreactors (12 hours) Mode of operation of reactors batch, CSTR, CSTR with Recycle, Fed batch and Reactors in series,Estimation of Biomass,substrate and product concentration in reactors - Nonideal reactors RTD studies.

Bioreactor Scale up (6 hours) Scaling up of lab scale bioprocesess to Industrial level

Text Books1. Pauline M.Doran, Bioprocess Engineering Principles,Academic Press,20052. Shuler, M. and F. Kargi. 2002. Bioprocess Engineering. Prentice Hall (I) Ltd.,3. Prescott, Lansing M.2005. Microbiology, 6th ed. NY: McGraw-Hill

Reference Books1. Reid and Rehm, 2003. Biotechnology Vol I to VIII. WILEY-VCH Verlag2. J.E.Bailey and D.F.Ollis,Biochemical Engineering,Mc-GrawHill International Publications,1986

3.

James M. Lee, Biochemical Engineering, PHI, USA.4. Peter F. Stanbury, Stephen J. Hall & A. Whitaker, Principles of Fermentation Technology,Science &Technology Books.

5. Harvey W. Blanch, Douglas S. Clark, Biochemical Engineering, Marcel Dekker, Inc.

Mode of Evaluation: Written Examination. Assignments, Seminars


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BIT 520L Bioprocess Technology

List of experiments

1. Screening of microorganisms for industrial applicationsa) Cellulase

b) Proteasec) Amylase

2. Growth curve and optimization studies.

3. Physical and chemical control of microorganisms.

4. Demonstration of biogas production

5. Production of fermented products wine, lactic acid, antibiotics.

6. Methylene blue reduction test to check the quality of milk samples

7. Assessment of water quality for physiochemical & biological parameters



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BIT 511 Immunotechnology 3 0 2 4

Objectives :

To prepare the students in the knowledge of prophylactic technology to prevent infectious diseases anddevelop strategies to treat allergic and autoimmune diseases.To orient the students to potential applications of bio-separation & molecular biology tools for developing

prophylactic & therapeutic agents against infections, autoimmune & allergic diseases.To review the industrial applications & explore the commercial potentials of these technologies.

Expected Outcomes :To meet the manpower need of healthcare biotechnology industry.To become a part of frontier areas of research in transplantation and immunological disorders.

Contents:

Immune system (10 hours)Immunity, cells and organs of immune system, immune responses, complement system and their pathways, biological consequences and diseases, cytokines and role of cytokines in signal transduction,diagnosis and therapy.

Antigen and Antibody (7 hours)Antigen structure and its types, antibody structure and its types, antigen processing and presentation,mechanism of antigen recognition, MHC organization Class I, II and III and MHC restriction.

Cellular Immunology (9 hours)T cell maturation, activation and proliferation. B cell maturation, activation and proliferation. T cellreceptors. Isolation of lymphocytes from the blood, Identification of lymphocytes based on CD markers,

Lymphoproliferation assay, Mixed lymphocytes reaction, HLA typing and cytokine assay IL 2, IFNgamma and TNF alpha

Immunopathology and Immmunotherapy (10 hours)Autoimmunity and autoimmune disorders, hypersensitivity reactions, transplantation and tumor Immunology, immunotherapy for tumors, immunotherapy for autoimmune disorders, vaccines activeand passive immunization, DNA and plant based vaccines, AIDS vaccine, recombinant antigen asvaccine, reverse vaccinology.

Immunotechnology (9 hours)Antibody engineering, catalytic antibodies, idiotypic antibodies, stem cell transplantation technology,Gene knock out animal models, Monoclonal antibodies and their use in diagnosis, ELISA, antigen

detection assay, plaque forming cell assay, immunoelectrophoresis, Immunoblottingprinciples andapplications,immunohistochemistry and Immunofluorescence, principle and applications.

Reference books :1. Kuby et al. 2007. Immunology. W. H. Freeman.2. Roitt et al. 2006. Essentials of Immunology 11th edition. Blackwell Publisher.3. Clackson,T. and Lwman,B.H.2004. Phage Display:A Practical Approach. Oxford University

Press.


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4. Andrew George, J T and Catherine Urch, E. 2000. Diagnostic and Therapeutic antibodies(Methods in Molecular Medicine) Humana Press.

5. John E. Coligan, Barbara Bierer, David H. Margulies, Ethan M. Shevach, Warren Strober andRichard Coico. 2006. Current Protocols in Immunology, Vo.1-5., John Wiley & Sons, Inc.

6. Moron. A. 2006. Immunotechnology:Principles, concepts and applications, John Wiley & Sons.7. Rao, C.V. 2003. Immunology. New Age Publication.

Method of Evaluation: Written Examination, Assignments, Seminars, viva voce and lab exam.


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BIT 511L Immunotechnology

List of Experiments

1. Immunodiffusion

2. Latex Agglutination test

3. Quantitative precipitn assay

4. Immuno-electrophoresis

5. Antibody labeling with enzyme

6. ELISA

7. Immuno-blot

8. Radio immuno assay

9. Immuno-histochemistry

10. Lymphocyte separation

Demonstration

Immuno-fluorescent microscopy



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BIT 513 Genomics and Proteomics 3 0 2 4

Course prerequisites: Knowledge on bioinformatics and molecular biology.

Objectives: This course will provide students advanced knowledge and practical skills needed for genomics, and proteomics. The emphasizes will be placed on Genomics and proteomics combine tofacilitate genome and proteome information acquisition, processing, storage, distribution, analysis andinterpretation of gene and protein function, further the studies will help to understand the techniquesinvolved in the analysis of genomics and proteomics and utilize the genomic and proteomic patterns inindustrial and medicinal diagnostics and treatment.

Expected Outcomes: Students will gain knowledge on the basics of genomics and proteomics and willgain confidence in taking up future research programs.

Introduction to Genomics (10 hrs)

Organization and structure of genomes, Genome size, Sequence complexity, introns and exons, Principlesof gene Expression, Genome Mapping, Human Genome Project, Genomes of other organisms, Genomestructure in viruses and prokaryotes.

Genome analysis (9 hrs)

Genome annotation, traditional routes of gene identification, detecting open-reading frames, Identifyingthe function of a new gene, gene ontology, comparative genomics, analysis, Protein structural genomics,introduction, determining gene function by sequence comparison, software programs for finding genes.

Introduction to proteomics (9 hrs)

Bridging Genomics and proteomics, Analysis of Proteomes, one dimensional and Two-Dimensional polyacrylmide gel electrophoresis, solubilization, Reduction, Resolution, Image Analysis of 2-DE Gels.Image visualization, spot detection and quantification. MALDI-TOF.

Proteome and genome analysis techniques (8 hrs)

Introduction to Mass Spectrometry, 2-D gel electrophoresis coupled with Mass Spectrometry, De novosequencing using Mass Spectrometric data, correlative Mass Spectrometric based identification strategies,Micro array techniques, types of micro arrays, Designing Micro array experiment, Micro array technologyin treating disease.

Applications in genomics and proteomics (9 hrs)

Genomics in Biopharmaceutical Industry. Functional Genomes, Pharmacokinetics, introduction toChemoinformatics: Genomics in relation to molecular Diagnosis, Molecular Therapeutic technologies.Gene Therapy, New Targets for drug discovery, Application of proteome analysis-drug development andtoxicology, Proteomics in drug discovery in humans, Glycobiology and proteomics in plant genetics and

breeding.

Text Book:Pennington, S. R. & Dunn, M.J. 2000. Proteomics - from Protein sequence to function. BIOS.Liebler, D.C. 2002. Introduction to proteomics - tools for new biology. Humana press.


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Reference Books:S.B.Primrose & R.M. Twyman. 2006. Principles of Genome analysis and Genomics. 7th edition.Blackwell Publishing.Sahai, S. 1999. Genomics and Proteomics. Functional and Computational Aspects. Plenum Publication.Richard Simpson. 2003. Proteins and Proteomics: A Laboratory Manual. CSHL press.

Mode of evaluation: Written examination, assignments, quiz and seminars


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BIT 513L Genomics and Proteomics

1. Genome mining2. ORF finding and gene prediction3. In silico analysis of genome islands4. In vitro analysis of genome islands5. Operon amplification6. Single dimension electrophoresis7. Solubilization of proteins for 2D gel electrophoresis8. Secretome analysis9. Membrane proteome preparation10. Iso-electric focusing11. 2D gel electrophoresis (demonstration)12. Mass Spectrometry (demonstration)



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BIT 537 Bioethics, Biosafety and Patents 3 0 0 3

Course prerequisites: Knowledge on bioscience and current legal issues related to it.

Objectives: This course seeks to identify and explore salient ethical, legal, and policy issues and possiblesolutions associated with developments in biotechnology. It also provides knowledge on biosafety andintellectual property rights (IPRs).

Expected Outcomes: Students will gain familiarity with some of the important ethical issues that arise asa result of advances in biotechnology and changes in biotech policy. Students will follow biosafetymeasures in research works & gain knowledge on patenting his or her research finding.

ContentsBioethics (10 hrs)Definition and scope of ethics, bioethics and business ethics, purpose, ideology and methodology,regulation, public evaluation of science and technology, unethical contexts for ethical questions: what isright and what are wrong, human subject protections, environmental ethics: anthropocentrism,

biocentrism, ecocentrism, ecofeminism, deep ecology, pluralism, communitarianism, land ethics,environmental health, environmental racism, environmental policy and law, epidemics: ethicalimplications and case studies (Tropical moist forest degradation, DDT and control of malaria, Pros andcons of nuclear power etc.,)

Ethical Issues (9 hrs)GM crops, patenting of genes for agricultural biotechnology, world food crisis, biofuels, environment and

biodiversity conservation, in vitro fertilization, embryo research, sex selection, surrogacy, geneticselection and enhancement, prenatal screening and gene therapy, patenting human genes, cloning of animals and humans, tissue engineering and transplantation, euthanasia & related issues, animalexperimentation in biomedical research & genetically modified microbes.

Biosafety (10 hrs)Guidelines, principles, risk criteria for establishing ascending levels of containment, agent summarystatements, biosecurity, biological risk assessment, laboratory and animal biosafety level criteria,laboratory biosecurity, occupational health and immunoprophylaxis. Status, safety and ecologicalassessment of drugs, vaccines, feeds, foods and food products produced by genetically modifiedmicroorganisms, plants and animals.

IPRs (9 hrs)History of IPRs in India, intellectual property, protection of IPR: Trade secret, Patent, Copyright, PVP,mask work protection, trademarks, cyber squatting, international harmonization of patent laws, WTO,TRIPs, India & TRIPs, protection of biotechnological inventions, patenting of genes, DNA sequences &genetic resources, patenting life forms, broad patents in biotechnology, biotechnology industry & patents,

patent imbroglio, PBR, policy on seed development & GEAC.

IPR protection, pros and cons (7 hrs)Choice of IPR protection, foreign patent protection, ownership and transfer of patent rights, employmentcontracts, management of IPR, benefits and problems from IPR, IPR and developing countries, ICBD,Indian response to IPR upheaval

Text/ Reference Books:Armitage, P. & G. Berry. 1994. Statistical methods in medical research. Blackwell sci. publication.Arthur W. Galston and Christiana Z. Peppard. 2005. Expanding horizons in bioethics. Springer.


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Casey Chosewood, L & D. E. Wilson. 2007. Biosafety in Microbiological and Biomedical Laboratories.5th edition. U. S. Government Printing Office.Howard B. Rockman. 2004. Intellectual property law for engineers and scientists. John Wiley & Sons,John A. Thomas and Laurie A. Myers. 1993. Biotechnology and safety assessment. Raven Press Ltd.,John Bryant, Linda Baggott la Velle and John Searle. 2002. Bioethics for scientists. John Wiley & SonsStephen G. Post.2004. Encyclopedia of Bioethics, 3rd edition. The Gale Group, Inc.

Mode of evaluation: Assignment, case studies, seminars & written examination


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BIT 522 Advanced Bioseparation Techniques 3 0 0 3

Course prerequisites: Knowledge on biochemical techniques.

Objectives: To provide an overview of the fundamentals of downstream processing for biotechnological processes. To understand the primary purification and isolation of proteins and product preparation.

Expected Outcome: Students will be expected to develop a strong basic foundation with a soundunderstanding of the major technologies employed in the processing of bioproducts. They will also beexpected to develop collaborative and disciplined work ethics while consistently addressing latest issuesin bioprocess technology. Upon successful completion of the course, individuals should possess thenecessary skills to qualify for employment in a variety of bioprocessing industries.

Contents

Overview

Overview of bioseparation processes, Characteristics of biomolecules and fermentation broths. Celldisruption methods, Primary purification, Separation of in-solubles, centrifugation, filtration andsedimentation.

Isolation of products

Solvent extraction, aqueous two-phase extraction, precipitation and adsorption. Membrane processes,dialysis, Ultra filtration, reverse osmosis and electrodialysis.

Chromatography

Principles of chromatographic separations, Gel filtration, Reversed phase, Ion exchange, Immobilizedmetal affinity chromatography and bio-affinity chromatography. Modes of operation, design and selectionof chromatographic matrices. Design and large scale chromatographic separation processes

Process

Final product purification and preparation, Crystallization, drying and lyophilization.Advances in bioseperations and case studiesReverse miceller extraction, super critical fluid extraction

Text books:1. Paul.A.Belter, E.L. Cussler and Wei shan Hu, Biosepation Downstream processing for Biotechnology Wily Inter Science Pub. (1988)2. Product Recovery in Bioprocess Technology, BIOTOL series, Butterworth Heinmann ( 1992)

Reference Books:1. J.Asenjo, Separation processes in biotechnology, Marcel- Dekker (1993)2. Verrall, M.S and Hudson M J separations for Biotechnology, Ellis Horwood Ltd., (1990)

Mode of Evaluation: Assignments, Written examination, seminar


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BIT 523 Food Process Technology 3 0 0 3

Course prerequisites: Knowledge on microbiology

Objective: The purpose of this unit is to familiarize the students with various aspects of food biotechnology. The course offers an integrated approach to technological aspects of Agro processing,Dairy processing and technology of fermented foods and finally on the recent trends in the field.

Learning out come: 1. Students should be able to use their knowledge on Biotechnology to thetechnology of food. 2. Understand basic process methods and appreciate role of biotechnology in

preserving foods. 3. Discuss the various biotechnological means of improving the food processtechnology and be able to understand the biotechnological basis for processing foods. 4. Understand thelatest trends in food biotechnology.

Contents:

Introduction to Food Biotechnology: (9 hrs)

Potentiality of food industry and the trade. Introduction to Food Biotechnology, history, importance,advances and Trends. Potential Impact of Biotechnology in Food Industries.

Agro Food biotechnology: (9 hrs)

Applications of biotechnology for agro produces and commodities. Impact of Biotechnology on Nutritional Quality of Food Plants. Spoilage of food, Microbiology of water, fruits, vegetables;Preservation Technology Fumigation, modified atmospheric storages, thermal and cold preservation,canning, dehydration, ultra filtration and irradiation.

Dairy Food Biotechnology: (9 hrs)

Applications of biotechnology in processing of dairy products. Specific Examples, Dairy Starter Culturesand Dairy Enzymes, Caseins etc. The different types of processing followed for dairy products including

pasteurization . Probiotics and health benefits of fermented milk and milk products.

Fermentated foods: (9 hrs)

Fermented foods : origin scope and development; Application of enzymes in food industry production of food flavor, colour, enzymes; Immobilized enzymes. Technological aspects of industrial production of

beer, wine, Organic acids, Amino acids; Vitamins; Antibiotics, Bakers yeast; Single cell protein;

Trends in Food Biotechnology: (9 hrs)

Recent Applications of Biotechnology in food industry. Biotechnology Applied to Fats and Oils and other ingredients. Regulatory and Social Aspects of Biotechnology with particular reference to GeneticallyModified foods. Consumer perspective and future of food biotechnology.

References:Jay, 1987. Modern Food Microbiology, CBS Publishers, 1987Ranganna, S. 1986. Handbook of Analysis and Quality Control for Fruits and Vegetable Products, TataMcGraw Hill, New Delhi.William C. Frazier, Food Microbiology fourth edition Tata Mc Graw Hill


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Michael P.Doyle Food Microbiology 2nd Edition, ASM press.Fennema, O.R. Ed. 1976. Principles of Food Science: Part-I Food Chemistry. Marcel Dekker, New York.

Mode of evaluation: Assignment, case studies, quiz, seminars & written examination


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BIT 529 Metabolic Engineering and Metabolomics 3 0 0 3

ObjectiveTo equip the students with the fundamental aspects and applications of metabolic engineering

Learning outcomesTo formulate flux mapsTo design experimental strategies for the evaluation of metabolic fluxTo find the optimal pathway for metabolic engineering

Introduction to enzyme expression and kineticsOverview of three dimensional structure of the enzyme enzyme kinetics: Michaelis-Menton and non-Michaelis Menton situations enzyme activity parameters factors affecting the enzyme kinetics enzyme inhibition modes regulation in enzyme production.

Metabolomics Need for metabolic mapping Global metabolite profiling Metabolic finger printing Metabolite targetanalysis metabonomics in vitro metabolomics advantages of metabolome nalysis metabolomicsand microarray techniques in metabolome analysis: capillary electrophoresis, FTIR, MS, NMR quantitative metabolomics.

Metabolic networks and flux mapsMetabolic pathways in amino acid production and their linkages with other metabolic cycles linear and

branched flux Synthesis of metabolic pathways formulation of flux maps stoichiometric modeling of cell metabolism

Metabolic control, flux balance analysisSystem classification for flux analysis - isotope labeling methods carbon metabolite balances connectivity theorems in metabolic control analysis determination of control coefficients constraint

based modeling incorporation of thermodynamics modeling metabolic networks computationalissues in analyzing biochemical networks

Approaches and case studiesTop-down and bottom up approaches metabolic engineering of plants for human health and nutrition hemicelluloic ethanol production application in food science metabolomic assisted breeding for cropimprovement molecular target based drug discovery discovering novel pathways production of exopolysaccharides.

Text Books No prescribed text book

References

Metabolic Engineering Principles and Methodologies. (GN Stephanopoulos, AA Aristidou and J Nielsen), 1998, Academic Press. Neurotoxicology (2008) 29: 1 12Journal of Chromatography A (2007) 1168: 237 246Acta Histochemica (2004) 106: 89 96Current opinion in Biotechnology (2002) 13:20 24.Trends in Analytical Chemistry (2008) 27: 228 237.Current opinion in Biotechnology ( 2003) 14: 491-496Biophysical Chemistry (2004) 109: 1 15.


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Computers and Chemical Engineering (2005) 29: 2297 2303Current opinion in Plant Biology (2000) 3: 249 253.


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BIT 514 Nano Biotechnology 3 0 0 3

Course prerequisites : Knowledge on biochemistry, biophysics and molecular biology.

Objectives: To make the students learn the basics of nanotechnology. Understand the various methods inthe synthesis / preparation of nanoparticles. Get exposed to potential applications of nanobiotechnology.

Expected Outcomes: At the end of the course the student should be able to suggest a suitabletechnique(s) to characterize a given nanoparticle. Appreciate the potential uses of nanobiotechnology.Design a nanobioparticle for specific application.

ContentsIntroduction to nanotechnology and Interphase systems (9 hrs)

Biocompatible inorganic devices, Lab-on-a-chip devices, Microcontact printing of proteins, Cellnanostructure interactions, Defined networks of neuronal cells in vitro

Nanoanalytics (9 hrs)

Lumuniscent quantum dots for biological labeling, Nanoparticle molecular labels, Surface Biology:Analysis of Biomolecular Structure by Atomic force microscopy and molecular pulling, Forcespectroscopy, Biofunctionalized Nanoparticles for surface enhanced Raman scattering and Surface

plasmon resonance

DNA-based nanostructures (9 hrs)DNA-Protein nanostructures, DNA-templated electronics, Biomimetic fabrication of DNA-based metallicnanowires and networks, Mineralization in Nanostructured Biocompartments: Biomimetic ferritins for high-density data storage, DNA-gold nanoparticle conjugates, DNA nanostructrures for mechanics andcomputing: Nonlinear thinking with lifes central molecule; Nanoparticles as non-viral transfection agents

Protein-based nanostructures (8 hrs)

Engineering nanopores, Genetic approaches to programmed assembly, Microbial nanoparticle production,Magnetosomes: Nanoscale Magnetic Iron Minerals in Bacteria, Polymer nanocontainers, Nanoparticle-

biomaterial hybrid systems for Bioelectronic Devices and Circuitry

Application of nanobioparticles and Nanotoxicology (10 hrs)

Bacteriorhodopsin and its potential in technical applications, Tissue engineering, Biological activities of nanoparticles, interactions with cells, biomolecules, immune system, cytotoxicity, cellular responses,

biocompatibility, reducing and eliminating nanotoxicity and ecotoxicology.

Text books:CM Niemeyer and CA Mirkin. 2004. Nanobiotechnology: Concepts, applications and perspectives.Wiley-VCH Verlag GmbH & Co., (A)M Kohler and W Fritzsche. 2004. Nanotechnology: An introduction to nanostructuring techniques.Wiley-VCH Verlag GmbH & Co.,

Reference books:Bhusan. 2004. Handbook of nanotechnology. Springer-Verlag.


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PM Ajayan, LS Schadler and PV Braun. 2003. Nanocomposite science and technology. Wiley - VCHVerlag, GmbH & Co.,Yuliang Zhao and Hari Singh Nalwa. 2007. Nanotoxicology.. American Scientific Publishers.

Mode of Evaluation: Written Examination, Assignments and tutorials


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BIT 527 Biobusiness Management 3 0 0 3

Objectives: T o make the students to Understand regulatory issues, clinical trial process for both devicesand drugs. Understand key issues surrounding a products intellectual property. Learn about theentrepreneurial mindset in startup small and large companies. Learn about bioethics issues in developing

and marketing biotech productsExpected outcome: At the end of the course the student should be able to perspective on the

biotechnology industry: current status and drivers; Explore issues in the transition from research project to product launch in the biotechnology and medical device industry

ContentsBiotechnology industry overview (8 hours)Bio business segments in India and world level. Case study and its importance. Trends and key issues in

biotechnology and devices industry. Technology basis in industry segments, Emerging technologies,technical convergence issues.

Product development (8 hours)

Marketing and public perceptions in product development. Product development and projectmanagement. Transition from R&D to business units. Innovation management, radical innovationtechnologies vs. stage gate approach in product development. Discussion on regulatory issues and clinicaltrial process.

Entrepreneurship (8 hours)Role of small scale industries in various segments of India, Growth of small scale industries in developingcountries and their positions large industries; role in the national economy; characteristics and types of small scale industries and large scale industries.

Project identification, Planning and control (8 hours) - assessment of viability, formulation, Evaluation, financing, field-study and collection of information,

Proposal of projects and preparation of project report. Laws concerning entrepreneur viz, partnershiplaws, business ownership, sales and income taxes and workman compensation act.

New Venture Creation (8 hours) New venture creation, technology transfer and business planning. Financing biotech businesses and project finance. Compare the role of Public companies and private limited companies. Way of enhancingfinancial status. Case discussion on Surface Logix entrepreneurship issues.

Text books:1. Eric Grace. 1997. Biotechnology unzipped: Promises and realities. Wash. DC: Joseph Henry

Press.2. Arthur Kornberg & Sausalito, CA 1995. The golden helix: University Science Books..

Reference Books:1. Richard Oliver. 2000. The coming biotech age: The business of biomaterials. NY: McGraw Hill.2. R E Bulger et al. 1993. The ethical dimensions of the biological sciences. NY: Cambridge Univ

Press3. David F. Betsch Principles of Biotechnology.4. Joseph, L. Massod, Essential of Management, Prentice Hall of India



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BIT 530 Plant Biotechnology 3 0 0 3

Course Pre-requisites: Molecular biology

Objectives: To give a broad idea on the possible scopes and commercial applications in the field of pant biotechnology

Expected outcome: Facilitates students in becoming an entrepreneur in the field of tissue culture and aresearcher in making transgenics.

Contents:Plant Genome Organization (9 hrs)Chromosome structure, organization, DNA (nucleus, mitochondria, chloroplast), gene structure,expression, regulation of gene expression, transcription, and translation post transcriptional and

post translational modifications gene families, model plants.

Plant Tissue, Cell and Organ Culture (9 hrs)Totipotency, plasticity, nutrients, plant growth regulators, cell culture explant, surface sterilization,media, culture conditions, single cell culture, protoplast culture, somatic hybridization, callus,organogenesis and embryogenesis, somaclonal variation, cell suspension culture, protoplast culture,meristem culture, embryo culture, microspore culture artificial seeds, hardening, applications.

Plant Genetic Engineering (9 hrs)Direct and indirect methods of transformation, gene manipulation vectors (Agrobacterium and viralvectors), nuclear and chloroplast transformation, gene silencing, marker free technology, metabolicengineering.

Transgenics, CMS and IPR (9 hrs)Transgenics development for overcoming biotic and abiotic stress, herbicide resistance, productiontraits, technology protection systems, male sterility, IPR patents, trade secrets, copyright, PBR.

Inducible control of gene expression (9 hrs)Promoter systems plant based developmental processes (senescence specific, ABA, hormoneresponse elements), environmental signals (wound inducible, nitrate inducibility, heat shock

promoters) and non-plant systems (tetracycline repressor, ecdysteroid, glucocorticoid and copper).

Text/Reference Books:1. Adrian Slater, Nigel Scott and Mark Fowler. 2003. Plant Biotechnology The genetic manipulation of

plants, Oxford University Press.

2. Old, R.W., and Primrose, S.B., 2001. Principles of Gene Manipulation - An Introduction to GeneticEngineering, Blackwell Science Ltd., New York.3. Bernard R. Glick and Jack J. Pasternak. 2001. Molecular Biotechnology Principles and Applicationsof recombinant DNA, ASM Press, Washington D.C4. Bhojwani, S.S., and Razdan, M.K., 2004. Plant tissue Culture: Theory and Practice, Elsevier SciencePublishers B V, Amsterdam, The Netherlands.

Mode of Evaluation Examinations, class tests, seminar and assignment.BIT 524 Natural Products Technology 3 0 0 3


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Course Pre-requisites: Knowledge on biochemistry

Objectives: To make the students to understand the natural products. Learn about technology to developnatural products and suitable utilization

Expected outcomes: At the end of the course the student should be able to know the different kinds of natural products.

ContentIntroduction (8 hrs)Importance of natural products - natural product chemistry classification of natural products. Collectionand processing of the plant material, methods of extraction - Purification and concentration

Identification. (8 hrs)Secondary Metabolites Chemistry, biological synthesis and types of Terpenoids, plant Sterols, glucosides,

phenolics and Alkaloids

Pigments (8 hrs)Plant pigments and natural dyes: Introduction, history, importance of plant dyes and mordants, chemistryand types, dye extraction and fabric dye process, Application of Technology for the production of naturaldyes and colourants. Biosynthetic pathway of carotenoids, flvaonoids and anthocyanins.

Oils (8 hrs)Essential oils, volatile oil, & Vitamins - Introduction classification source description Biosynthesis- active constituents. Sources and toxic manifestations of poisonous plants.

Herbal Products (8 hrs)

Medicinal plant and herbal practice in India Introduction History - Herbal Practice - Study of different

traditional medicine Conservation sustainable utilization.

Reference

Harborne J. B. 1998. Phytochemical methods- A guide to modern techniques of plant analysis, 3rdEdition., Chapman & Hall.Keith Wilson and Walker 2000. Practical Biochemistry- Principles and Techniques, 5TH edition.,Cambridge University Press.Bell. F.A., Harlwood B.V. 1980. Encyclopedia of Plant physiology Secondary Plant ProductsStumpt P.K. and Conn. E.E. 1981.The Biochemistry of Plants Secondary Plant ProductsGoodwin T.W. and Mercer E.I. 1972. Introduction to Plant Biochemistry. Pergamon Press, Oxford

Mode of Evaluation: Quiz, Written Examination, Assignments and tutorials


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BIT 536 Medical Biotechnology 3 0 0 3

ObjectivesTo highlight and specialize the basic concepts involved in applications of Medical Biotechnology for human welfare.

Expected OutcomeStudents will acquire knowledge, understanding and skills about the association of microbes and non-microbial ailments and their treatment strategies.

III. Human Anatomy and PhysiologyIntroduction to Human Anatomy and Physiology Major systems Respiratory Circulatory Digestive

Excretory Nervous and Sensory systems.

IV. Diseases burden an over viewMicrobial Bacterial, Viral, Fungal and Parasitic diseases. Investigation of epidemics. Methods of culturing and assaying Bacteria, Fungi, Virus and Parasites Mode of action of antibiotics.

V. VaccinesAn outline Allergy, Immunoprophylaxis, vaccines, monoclonal antibodies and its therapeuticapplications.

VI. Transplantation and teratogenesisEmbryo culture, stem cell culture organ culture artificial blood.

VII. DiagnosticsClinical biochemical and immunological therapeutic categories such as vitamins, laxatives,analgesics, non steroidal contraceptives, antibiotics and biological hormones.

Text Book1. Gary Walsh (2003), Biopharmaceuticals: Biochemistry and Biotechnology by; ISBN 0470843276

Reference Books

1. Crommelin, Sindelar (1997), Pharmaceutical Biotechnology, Harwood Academic Publishers.2. Leon Lachman (1776), Theory and Practice of Industrial Pharmacy, 3rd edition, Lea and Febiger.3. Alfonso R. Gennaro (2003), Remington: The Science and Practice of Pharmacy, publishers lippincottWilliams & Wilkins; 20th edition ISBN: 0781750253.4. Review of Medical Physiology (LANGE Basic Science) (2003) by William F. Ganong publishers,McGraw Hill Medical; 21st edition, ISBN:0071402365.

Mode of Evaluation: By Assignment, Seminar and Written Examinations.


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BIT 533 Environmental Biotechnology 3 0 0 3

Objectives:To explore the different facets of environmental biotechnology.The students will be able to understand the theories and fundamentals underlying environmentalengineering and sciences.

Learning Outcomes:To understand the fundamentals of environmental microbiology with main emphasis to soil microbiology.To learn about the degradation outcomes of different environmental xenobiotics.To have an insight about environmental engineering ETP treatment trains and design of activated sludge

process.Treatment of industrial wastes emanating from different sources.

Contents:

Fundamentals of Micro-organismsThe lithosphere, atmosphere and hydrosphere as a microbial habitat, Ecological adaptations, Interactionsamong soil microorganisms, Biogeochemical role of soil microorganisms (Carbon, Nitrogen, Sulphur).

Degradation of xenobiotic compoundsSimple aromatics, aliphatics, Chlorinated polyaromatic compounds, petroleum products, pesticides andsurfactants.

Wastewater managementWastewater characteristics, biological wastewater treatment, unit operations, design and modelling of activated sludge process, design and modelling of trickling filter.

Treatment of industrial wastesDairy, pulp, leather, petrochemicals, distilleries, Solid waste management.

Special topics in environmental biotechnologyMicrobial diversity, Biofouling, biocorrosion, Bioleaching, acid mine drainage.

Text Book1. Foster C.F., John Ware D.A., Environmental Biotechnology, Ellis Horwood Ltd., 1987.

Reference Book1. Stanier R.Y., Ingraham J.L., Wheelis, M.L., Painter R.R., General Microbiology, McMillanPublications, 1989.2. Metcalf and Eddy, Wastewater Engineering-Treatment, disposal, reuse, 3rd Edn. Tata Mc. Graw Hill.3. Kernely D., Chakrabarty K., Omen G.S., Biotechnology and Biodegradation., Advances in applied

biotechnology series, Vol. 4, Gulf Publications Co., London, 1989.

Mode of Evaluation: Written examinations, Assignments, Seminars, Quiz.


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BIT 502 Industrial Biotechnology 3 0 0 3

Course Pre-requisites: Biochemistry

Objectives: At the completion of the subject, students will have an understanding.

1. Biological systems and molecules and how these are harnessed in biotechnology2. Understanding of the wider influences on the bio-processing industry

Expected Outcome: Students will be expected to develop a strong basic foundation with a soundunderstanding of the major technologies employed in the processing of bioproducts. They will also beexpected consistently addressing latest issues in bioprocess technology. Upon successful completion of the course, individuals should possess the necessary skills to qualify for employment in a variety of bio-

processing industries.

ContentsFermentation technology (9 hours) Fermenter design, aeration agitation, mass transfer in fermentation, Reactors of immobilized enzymes:

design and application, fermentation kinetics, screening techniques of microorganisms, computerizedcontrol of fermentation process, system configuration.

Scale up Process (9 hours) Scale up of fermentation processes: principles, theoretical considerations and techniques used, Productrecovery, isolation and purification of fermentation products: filtration, solvent extraction, adsorption and

partition, paper/gas/thin-layer and ion-exchange chromatography, electrophoresis and counter-currentdistribution, crystallization, turbidity analysis and cell yield determination, disintegration of cells for

product recovery

White biotechnology products (9 hours)Organic solvents, Organic acids, Antibiotics, vitamins, Amino acids, Nucleotides, Enzymes,

Biopolymers.

Enzymology (9 hours) Enzyme sources and properties. Enzyme kinetics, enzyme regulation, applications of enzymes: biological

preparations/ analytical reagents, diagnostics, therapeutics, Techniques of immobilization of enzymes andtheir applications in industry, Kinetics of immobilized enzymes

Business organization and finance in industrial biotechnology (9 hours)Organization of biotechnology industry, Public Policy Opportunities, Entrepreneurial Opportunities,Investment and Investment Management Opportunities. Financial risks.

Reference Books:

1. Win Soetaert and Erick J. Vandamme. Industrial Biotechnology sustainable growth andeconomic success, 1 st Edition, John Wiley and Sons Publications, 2010.2. Julio Polaina and Andrew P. Maccabe, Industrial enzymes structure, function and applications,Springer publications, 2007.3. Fabrizio Cavani, Sustainable industrial processes, John Wiley and Sons Publications, 2009.4. Nduka Okafor, Modern industrial microbiology and biotechnology, Science Publishers, 2007.5. Jacobsen and A. I. Werthei, Modern pharmaceutical industry, Jones and Barlett Publishers, 2010.

Mode of Evaluation: Written Examination, Assignments and seminars


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BIT 515 Protein Engineering 3 0 0 3

Course Pre-requisites: Knowledge on biochemistry, physical chemistry and molecular biology

Objectives : To make the students to understand the basics of protein three-dimensional structure:Correlate a given protein structure to its function; Get acquainted with the recent developments in proteinengineering techniques; Get familiarized with basic strategies involved in protein design

Expected Outcome: At the end of this course the student will be able to analyze the three dimensionalstructure any given protein; Correlate the structure of a given protein with its function; Design rationalapproach to engineer a given protein so as to exhibit required function

ContentsIntroduction to protein structure and properties (8 hrs)Basics of proteins, classification and properties of proteins, levels in protein structure, motifs in proteinstructures, posttranslational modifications of proteins, covalent chemical modification of proteins, moderntechniques in protein sequencing.

Protein stability, folding and dynamics (10 hrs)Factors determining the intrinsic and extrinsic stability of proteins, thermodynamic stability versus kineticstability of proteins, unfolding and folding of proteins, induced molecular conformational changes in

proteins, molecular dynamics of proteins, techniques for the study of proteins structures, UVspectroscopy, Circular dichroism, Fluorescence, Mass spectrometry, Nuclear magnetic resonancespectroscopy, X-diffraction technique, prediction of protein structure from sequence data.

Structure function relationship of proteins (9 hrs)Architectural proteins, Nucleotide and Nucleic acid binding proteins; Fibroblast growth factor, epidermalgrowth factor as examples of receptor-protein interactions; phosphorylation and dephosphorylation assignaling events; apoptosis for protein interaction network. Functional proteins, proteases, lipases,oxidoreductases, amylases.

Strategic design in the production of recombinant proteins (9 hrs)Types of mutagenesis, over-expression of proteins, differences in the host cells, molecular evolutiontechniques, high throughput production and analysis of recombinant proteins, inclusion bodies, co-expression of proteins with specific properties, protein renaturation, stabilization of proteins, productionof chimeric multifunctional proteins.

Protein engineering by semi-synthesis and covalent modification (9 hrs)Susceptibility of amino acid side chains for chemical modification; residue specific modifications;reagents for modifications; monofunctional, bifunctional and polyfunctional cross linkers; modificationusing carbohydrate polymers; fragment condensation route in semi-synthesis; insulin and cytochrome csemi-synthesis; press-stud conjugations; clinical and industrial applications of protein conjugates.

Text BooksC.Branden and J.Tooze. 1999. Introduction to Protein structure, Garland Publishing Inc.,Thomas E Crieghton. 2002. Proteins Structures and molecular properties, WH Freeman and Company.Carmicheal JA Wallace. 2000. Protein Engineering by semisynthesis, CRC Press.

Reference Books David Whitfield. 2005. Protein: Structure and Function, John Wiley and Sons.Jeffrey L Cleland and Charles S Craik. 1996. Protein Engineering: Principles and Practice, John Wileyand Sons.


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Mode of Evaluation Examinations, class tests, seminar and assignment.


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BIT 531 Animal Biotechnology 3 0 0 3

Objectives: Animal cells in biotechnology focuses primarily in gene transfer in cells for their GEOs areGMOs products development.

Expected outcome: Enables student to think of animal cells, tissue and organ improvements throughmolecular breeding by gene transfer and by means of other cell & tissue culture techniques..

ContentsLaboratory and introduction to cells (10 hours)Equipments and Materials for animal Cell Culture Technology:Design of Tissue Culture Laboratory, Equipments: Laminar Flow Hoods, CO2 incubator, Microscopes,Centrifuge, Refrigerators and Freezers, Pipetting aids, Miscellaneous small items of Equipments,Materials, filter, Miscellaneous, Items, Basic Aseptic Techniques, .Storage shipping and safety.Characters of cells and behavior:Cells and tissue types, Behavior of cells in culture: Primary cell lines permanent/Established celllines/Transformed cell lines, Tumor/cancer originated cells.

Growth media (10 hours)Physical requirements and Nutritional Requirements of Cells and growth media and cell culture growthkinetics; Natural media, Basal salt solution (BSS)-Various types, Minimum Essential Medium (MEM),Antibiotics in media, Serum dependent defined media, Serum independent defined media Cell specificmedia, and bulk ions, trace ions, CO2, O2 tension, Ascorbic acid, sugars, and Vitamins coenzymes. Basicof mammalian cell culture, open and closed cell-cultures, Primary Cell culture Isolation and separationof cells, viable cell count, maintenance of cell culture, maintenance of stock culture, Antibiotic free stock cultures,Types of cell cultures Monolayer, Suspension, Clonal culture, Mass culture, micro carrier culture (monolayer), and Stem cell cultures (ESC)

Biology and Characterization of cultured cells (9 hours)

Karyotyping, Contamination Testing of Culture, Viability measurement and cytotoxicity, Measurement of growth parameters, Cell cycle analysis and Synchronization of cultures, Cell surgery Methods,Preparation of anucleated cells and polykaryon cells, Preparation of ghost RBCs, Preparation of minicells, micro cells, surgical manipulation of in vitro fertilization.

Cell Fusion Methods (9 hours)Cell fusion techniques, Hybridoma cell preparations and their properties, Use of Hybridoma technology:e.g. M AB and other related techniques,

Use of Animal Cells in Culture-(9 hours) Mutant cell preparation, Evaluation of Chemical carcinogenicity, Cell malignancy testing, ToxicityTesting, Karyotyping and cytogenetic characterization, Production of metabolic products, ESC

applications, Pluripotent stem cell applications ESC applications, Pluripotent stem cell applications ESCapplications, Pluripotent stem cell applications

Text Books1. Butler M. 2004. Animal Cell Culture and Technology, BIOS Scientific Publishers, Second edition.2. Kuchler, R.J. 1977. Biochemical Methods in cell culture and Virology, Dowden, Huchinson andRossini. Strausberg, USA,3. Morgan, 1993. S.I.Animal cell culture, ,Bio Scientific Publishers Ltd, Oxford.


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Reference Books1. Freshney,R.I.Culture of Animal cells: A Manual of Basic Technique, 1994, John Wiley and Sons Inc.Publication, USA.2. Butler, M.Mammalian, cell Biotechnology: A Practical Approach (1991), IRL Press, Oxford.3. Jenni P.Mather and David Barnes, eds; Animal cell culture Methods, Methods in cell Biology, vol.57,Academic Press.4. Cell Culture: Methods in enzymology,Vol-58,Academic Press 1979 or recent



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BIT 532 Pharmaceutical Biotechnology 3 0 0 3

Objectives: To create awareness on pharmacology and biotechnology based pharmaceutical products

To promote the applications of biotechnology to the thrust areas of healthcare industry

Expected outcome : Students will gain enough knowledge to take up pharmaceutical biotechnology as aresearch career to develop newer productsTo provide manpower to the biotechnology based pharmaceutical companies, clinical trial industriesPrepares students to aspire as consultants in KPO healthcare industry

General pharmacology (5 hours)Introduction - Development of Drugs, Pharmacokinetics and pharmacodynamics, routes of drugadministration Bioavailability curve, Drug receptor interaction, adverse drug reaction, prescription.

Manufacturing Principles General Considerations (12 hours)

Major classes of therapeutic agents (anesthetics, opioids, antiinflamatory, antihistamines, lipid loweringdrugs,antibiotics) and their manufacturing; Compressed tablets, wet granulation-dry granulation or slugging-direct compression-tablet presses, coating of tablets, capsules, sustained action dosage forms-

parenteral solutions-oral liquids-injections-ointment-topical applications.

Formulating protein drugs (10 hours)Pharmacokinetics and pharmacodynamics of peptide and protein drugs, Microbial decontamination,excipients used in biotech products, self life of protein based drugs, routes of administration andabsorption enhancement in protein drugs and packaging considerations, controlled and site specificdelivery of protein drugs, Critical analysis of peer-reviewed papers in peptide and protein drugs.

Protein drugs - therapeutic aspects (10 hours)

Pharmacotherapy using Cytokines, interleukins and interferon-gama, insulin and insulin analogs indiabetes treatment. Growth hormone, Modern vaccines -subunit vaccines, r-DNA vaccines, Developmentof antibody based drug therapy, monoclonal antibody, murine antibody, chimeric antibody, humanizedantibody. Biotechnological strategies to overcome drug resistance. Critical analysis of peer-reviewed

papers on drugs developed by Biotechnological means.

Drug Development (8 hours)Overview of drug discovery, HTS strategies, Assessment of drug safety, Clinical Trials & Regulations -Clinical Trials Design, double blind studies, placebo effects, FDA regulations (General) and IndianDrug regulations- highlights.

Textbooks:

1.

Swarbrick Remingtons Pharmaceutical Sciences, Mack Publishing and Co. 18 th eds. 1996

Reference books:1. A C Guyton ,Text Books of Medical Physiology, WB Saunders, Hong Kong,19862. Pamela C.Champe and Richard A.Harvey, Lippin cotts Illustrated reviews BIOCHEMISTRY

2nd ed.3. R S Satoskar, Pharmacology and Pharmacotherapeutics, 20 th Edition, 2007.4. Leon Lachman et al Theory and Practice of Industrial Pharmacy , 3 rd Edition, Lea and Febiger,

1986


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5. Daan J A Crommelin and Robert D Sindelar, Pharmaceutical Biotechnology, 2 nd Edition, 20026. Gary Walsh, Biopharmaceuticals: Biochemistry and Biotechnology, Wiley, second edition. 2003.7. Rang HP: Drug Discovery and Development Technology in Transition, Churchill Livingston,

Elsevier, 20068. Swarbrick Remingtons Pharmaceutical Sciences, Mack Publishing and Co. 18 th eds. 19969. Recent peer reviewed journal articles

Mode of Evaluation: Written examinations, Seminar, Quizzes, Assignments.


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BIT 525 In silico Drug Design 3 0 0 3

Course Pre-requisites : Knowledge on biochemistry and bioinformaticsObjectives: This course aims to study the practical aspects of mathematical and statistical modelling,focussing on the biological sciences and complex systems.Expected Outcome: After taking this course, the student should be able to construct an initialmathematical model for a real-world process and assess this model critically

Content

OverviewObjectives of mathematical and statistical modelling; the iterative nature of modelling; Introduction to

basic Mathematical and Statistical Techniques (recapitulation): Algebra (matrices, determinants,eigenvalues); Theory of equations (Polynomials, Finding Roots); Discrete and Continuous Equations:Ordinary differential equations (both linear & non-linear); Partial differential equations: Equilibria andstability analysis (asymptotic behaviour); Dynamic behaviour: bifurcation, limit cycle, chaos; Probabilitytheory; Elementary Statistics: Central tendency, Deviations, Correlation & Regression, Statisticalestimation & inference and Testing of hypothesis: Optimisation techniques. (All the above topics should

be based on examples taken from different biological situations)

ModellingThe three stages of modelling. (1) Model formulation, including the art of consultation and the use of empirical information. (2) Model fitting. (3) Model validation. Construction of models mathematicaland statistical approach. Introduction to different types of models from biological perspectives: Cellkinetics: Bio-chemical reactions, oscillators, feedback control mechanisms: Gene regulatory models,metabolic pathways, signal pathways:Ionic processes, Hodgkin-Huxley models :Epidemic models, host-

parasite interaction, disease dynamics etc : Ecological systems: population dynamics, Interacting species(prey-predator, symbiosis, competition etc.). databases STKE biocarta

StatisticsModel fitting and model validation; Data analysis, Test of significance, Curve fitting (use of regression,linear/non-linear), Best fitted curve, Prediction, Accuracy, Parameter Estimation. (Use different types of data sets to give examples).

System analysisStochastic Processes (Random walk or Markov process); Effect of randomness on different biologicalsystems/parameters, different types of noises.: Stochastic Differential Equations (Ito Eqns., Fokker-Planck Eqns.), : Reaction-diffusion Systems, : Modelling mean response and variability; Probabilisticmodels for complex systems; Comparison of theoretical and experimental models.

Case studiesData analysis, Time series, non-linearity (examples from epidemiological studies, experiments, reactionkinetics, neurobiology, etc.); Numerical simulation; Introduction to Computer programming/Software.

Software Packages: EXCEL, SPSS, NEURON, MATLAB,

Text Books: Anderson, R.M. and May, R.M. 1992. Infectious Diseases of Humans: Dynamics andControl, Oxford University Press, Oxford.Armitage, P. and Berry, G. 1994. Statistical Methods in Medical Research, Blackwell Sci. Publication.


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Reference Books: Clark, C.W. 1990 Mathematical Bioeconomics- The optimal management of renewable resources, Wiley Eastern, New York.Dunn, O.J. & Clark, V.A. 2001 Basic Statistics: A Primer for the Biomedical Sciences, John WileyEdelstein, L. 2005 Mathematical Models in Biology, SIAMCambridgeUniversity Press.Farkas, M. 2001 Dynamical Models in Biology, Academic Press, NY.Gupta, S.C. and Kapoor, V.K. 2003. Fundamental of Applied Statistics, S.Chand & Sons, Delhi.

Mode of Evaluation: Quiz, Written Examination, Assignments and tutorials


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BIT 534 Systems Biology 3 0 0 3

ObjectivesIt aims at system-level understanding of biology, and to understand biological systems as a system. Thismeans an examination of the structure and dynamics of cellular and organism function, rather than thecharacteristics of isolated parts of a cell or organism.

Learning outcome:The biological knowledge is growing very rapidly, and data analysis can hardly keep pace. In the

bioinformatics area, tools have been developed and will be advanced to handle the huge and rapidlygrowing amount of data stored in databases. One main effort is to group and compare data, to gaininformation about single molecules compared to similar molecules.

Contents:System-level Understanding of Biological SystemsIntroduction, system, level understanding of biological systems. Advanced measurement systemsmodeling genetic networks.

Modeling the Activity of Single GenesModeling the activity of single genes, a probabilistic model of a prokaryotic gene and its regulationmodeling biochemical networks, atomic, level simulation and modeling of bio-macromolecules.

I. Kinetic Models

II. Kinetic models of excitable membranes and synaptic interactions, stochastic simulation of cellsignaling pathways, analysis of complex dynamics in cell cycle regulation.

Virtual Biology Laboratory

Modeling large biological systems from functional genomic data: Parameter estimation, cellular simulation, towards a virtual biology laboratory, computational cell biology, the stochastic approach.

Simulation of the Whole CellComputer simulation of the whole cell, computer simulation of the cell, human erythrocyte model and itsapplication, software for modeling and simulation, E-cell, and V-cell.

Textbooks:1. Hiroaki Kitano. Foundations of Systems Biology (Editor), MIT Press, 2001.2. Computational Modeling of Genetic and Biochemical Networks, James M. Bower, Hamid Bolouri,MIT Press, 2000.3. Gene Regulation and Metabolism: Postgenomic Computational Approaches, Julio Collado- Vides(Editor), Ralf Hofestadt (Editor), MIT Press, 2002.

Reference Books:1. Lars Skyttner General Systems Theory, Science, 2001.2. Dynamical Systems and Their Applications in Biology by Fields Institute for Research in MathematicalSciences. Science. 2003.

Mode of Evaluation: Written examinations, Seminar, Quizzes, Assignments


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BIT 506 Applied Bioinformatics 3 0 2 4

Objectives: Overview of bioinformatics, the application of computational methods to interpret the rapidlyexpanding amount of biological information. Following the natural flow of information in the cell, the

course begins with the analysis of gene sequences and progresses to the study of protein structures.

Expected Outcome: Students will gain knowledge on the computational analysis of gene sequences and protein structures on a large scale. Students will understand and implement bioinformatics algorithms.

Introduction to BioinformaticsIntroduction to operating systems, programming languages and RDBMS. Internet Protocols FTP Telnet and HTTP Internet domains.

Sequence alignmentSequence analysis Dot Plot, pairwise sequence comparison, BLAST and FASTA - multifunctional toolsfor sequence analysis. Multiple sequence alignments, Introduction to Phylogenetic analysis rooted and

unrooted trees construction of phylogenetic trees by Feng and Dolittle methods.

Biological databases Nucleic acid GenBank, EMBL, DDBJ, OMIM, ENTREZ. Protein Uniprot, Structural PDB.Metabolic pathway databases- KEGG pathway. Submitting sequences to databases BankIt, SequIn,WebIn, and Sakura. Sequence retrieval by Entrez.

Application of Bioinformatics in BiotechnologySequence annotation and tools for sequence annotation - GenScan, GRAIL, FGENESH/FGENES.Protein structure prediction secondary structure prediction Chou fossman algorithm. 3D structure

predictions Homology modelling fold recognition and Ab-initio methods.

Bioinformatics in Industry and other applicationsDrug designing - Lead compound CADD Pharmacogenomics- Molecular Medicine-Micro arraytechnology, Problems and challenges in bioinformatics.

Text Books:1. Discovering Genomics, Proteomics and Bioinformatics. A.Malcolm Campbell and Laurie J. HeyerCold,Spring Harbor Lab Press.(2002).

Reference Books1. Lesk, Arthur M. (2004). Introduction to protein science: architecture, function and genomics . OxfordOxford University Press.2. Lesk, Arthur M. (2007). Introduction to genomics . Oxford [Oxfordshire]: Oxford University Press.

3. Bioinformatics: Sequence and Genome Analysis. Author : David W. Mount Published : June 2004Publisher : Cold Spring Harbor Laboratory4. Essential Bioinformatics Jin Xiong, Texas A & M University5. Mount (2001) Bioinformatics Cold Spring Harbor Laboratory Press.

Mode of Evaluation: By Assignment, Seminar and Written Examinations


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BIT 506 Applied Bioinformatics

1. Retrieval of biological sequences Amino acid sequence, DNA sequences from database.

2. Pair wise sequence alignment using Dot plot.

3. Pair wise sequence alignment using dynamic programming.

4. Multiple sequence alignment using ClustalW.

5. Construction of phylogenetic tree.

6. RNA secondary structure prediction.

7. Introduction to DEEP-VIEW

8. Secondary structure: alpha helices and beta sheets

9. Prediction of secondary structure.

10. Homology modeling.



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Microbial Technology 3 0 0 3

Course prerequisites: Cell Biology and Biochemistry.

Objectives: To equip students with knowledge on usage of microorganisms in various fields of

biotechnology including food, agriculture, health and environment.

Expected outcomes: Students will gain knowledge on the applications of microorganisms and theconversion of microbiology to technology so that they can take up research career in this field.

Overview of microbial technology 9 Hrs Microbiology to Microbial Technology, Useful microbes fungi, bacteria, virus, algae, Classes of

products obtained from microbes and their uses, Microbes used directly as products, novel metabolitesfrom microbes.

Microbes for agriculture 9 Hrs Biofertilizers, classes of biofertilzers, mass production of biofertilizers, field application techniques,

marketing. Biopesticides, microbes used as biopesticides, mass production, field application andeconomics, PGPR microbes, microbial endophytes

Microbes for environment 9 Hrs Microbes for pollution control, immobilization of microbes, Bioremediation strategies for

bioremediation, biofilm based removal, quorum sensing, usage of microbes from extremeenvironments, microbes in waste management, water treatment,, bioleaching, biosorption

Microbes for nutrition and health 9 Hrs Antibiotics, commercial production of antibiotics, industrial production of vaccines, microbes in

probiotics and prebiotics, microbes as food and food additives, mushrooms and commercial production,microbial transformation of biochemicals, cosmetic microbiology.

Contemporary concepts in microbial technology 9 Hrs Microbial biosensors, microbial biofuel, electricity from bacteria, bacterial rhodopsin in IT industry,microbes to contain oil spills, model microbes in biotechnology research, microbes in nanobiotechnology.

Reference books:1. Antonio Mendez Vilas, Current topics in applied microbiology and microbial biotechnology, IstEdition, World Scientific Publishers, 2009.2. Ralph Mitchell and Ji-Dong Gu, Environmental Microbiology, 2 nd Edition, Wiley-Blackwell Science,2010.3. Trivedi, P.C., Microbes Applications and effects, 1 st Edition, Aavishkar Publishers, 2009.4. Heribert Insam, Microbes at work From waste to resources, 1 st Edition, Springer, 2010.

5. Nduka Okafor, Modern Industrial Microbiology and Biotechnology, 1st

Edition, Science Publishers, New Hampshire, USA, 2007.6. Alexander N. Glazer and Hioshi Nikaido, Microbial Biotechnology, 2 nd Edition, Cambridge UniversityPress, 2007.



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Marine and Aquatic Biotechnology 3 0 0 3

Course prerequisites: Molecular Biotechnology

Objectives: To obtain better knowledge of diverse environments, their biotechnological importance and

different biotechnological tools which are being employed to improve the quality and health of environment.

Expected outcomes: The students will gain knowledge in marine and aquatic environments, living thingsin these environments and the possible biotechnological approaches for commercial biotechnology in thisfield. The course will help students to take up employment as well as research in this field of

biotechnology.

Introduction to marine and aquatic environments 9 HrsImportance of Fresh and Sea Water - World Oceans, Seas and Rivers Physical and Chemical Propertiesof water Abiotic and Biotic Factors of an Environment Ecological Divisions of the Sea Biogeochemical Cycles Food Chain and Food Web.

Biological resources assessment 9 HrsMethods of Collection, Enumeration (Total and Viable Counts), Isolation, Culture and Identification,Methods of Studying the Marine Organisms and their Community Structure- Biochemical and Molecular Approaches-Microbial Processes- Influence of Environment Factors on Microbial Growth, Activity andDistribution, Fisheries and other Aquatic Potential.

Biotechnological potential of marine organisms 9 HrsDrugs from Microorganisms, Nanomaterials from Plankton and Crustaceans, Bioplastics and Biofuelfrom Aquatic Organisms, Adhesive Proteins from Bivalves, Biomaterials from Sponges, Ascidian andother Marine Organisms.

Biotechnological potentials from extreme environments 9 HrsImportance of Extreme Environments (Hot springs, Polar Regions, Hypersaline and AcidicEnvironments) Environmental Parameters - Adaptation and Survival of Microorganisms -Psychrophilic, Halophilic, Thermophilic, Acidophilic Microorganisms, Hydrothermal Vents - TubeWorms, and Chemosynthetic Bacteria, Microbes and Ultra-Low Frozen Invertebrates from Deep-SeaHydrothermal Vents and Cold-Water Sulfide/Methane Seeps

Biotechnology in aquaculture 9 HrsGlobal Perspective of Aquaculture, Genetically Modified Organisms and Aquaculture, Feeds Technology,Molecular Based Tools for Disease Diagnosis in Cultivable Organisms, Biotechnology in Aqua-HealthManagement.

Reference books:1.New Developments in Marine Biotechnology, Y. Le Gal, and H.O. Halvorson (Eds). Springer; Softcover (2010).2.Frontiers in Marine Biotechnology [Hardcover] Peter Proksch and Werner E.G. Mller (Eds.) Taylor

& Francis; 1 edition (2006) .Aquaculture Microbiology and Biotechnology, Volume Two . Didier Montet and, Ramesh C.

Ray (Eds.) Science Publishers; 1 edition (2011). 4.Introduction to Marine Biology. George Karleskint, Richard Turner, and James Small (Eds.) Brooks

Cole; 3 edition (2009).


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5.Bioactive Marine Natural Products [Paperback]. Dewan S. Bhakuni and, D.S. Rawat (Eds.)) Springer;Softcover, (2010)

Mode of Evaluation: Written examination, assignments/projects and seminars.


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Biodiversity and Conservation 3 0 0 3

Objectives : To impart knowledge on the biodiversity, concepts of biodiversity and its importance.

Expected Outcomes : Students will be able to understand the concepts of biodiversity in living organismsand the importance of conservation of biodiversity.

Introduction to biodiversity (9 hrs)Definition, history, importance of biodiversity, bioresources of India, biodiversity values.

Species and genetic diversity (9 hrs)Definitions, origin of species, speciation, molecular taxonomy, genetic diversity, flora and faunadiversity, molecular tools used for assessing the diversity.

Conservation biodiversity (9 hrs)IUCN, Red list, protected areas-National sanctuaries, National parks, Bioreserves, germplasm collection,in situ and ex situ conservation, conservation of sacred grooves, sustainable utilization, global climaticchange, green house gases, effect of global warming, mitigation of global warming.

Ecobiodiversity (9 hrs)Forest ecosystem, wet land ecosystem, mangrove ecosystem, arid and semi arid ecosystem, aquaticecosystem, organisms of extreme environments, insular ecosystem

Biodiversity and biotechnology (9 hrs)M

Documents

MTech (Biotechnology) Curriuclum & Syllabi for AY2012-13