Upload
tatiana-fitzgerald
View
44
Download
5
Embed Size (px)
DESCRIPTION
Ocular drugs influence on the structure of ocular tissues. Anzhelika S. Orlova, Tatyana G. Kamenskikh, Ivan D. Kamenskikh, Igor O. Kolbenev, Saratov State Medical University Alexey N. Bashkatov, Elina A. Genina, Artem A. Aratov, Anastasiya M. Parkheychuk, Valery V. Tuchin - PowerPoint PPT Presentation
Citation preview
Saratov State University
______________________________________________
Department of Optics & Biophotonics
__________________________________________________
Saratov Fall Meeting 2011
Ocular drugs influence on the structure of ocular tissues
Anzhelika S. Orlova, Tatyana G. Kamenskikh, Ivan D. Kamenskikh,
Igor O. Kolbenev,
Saratov State Medical University
Alexey N. Bashkatov, Elina A. Genina, Artem A. Aratov,Anastasiya M. Parkheychuk, Valery V. Tuchin
Department of Optics and Biophotonics of Saratov State University
e-mail: [email protected]
Anzhelika S. Orlova, Tatyana G. Kamenskikh, Ivan D. Kamenskikh,
Igor O. Kolbenev,
Saratov State Medical University
Alexey N. Bashkatov, Elina A. Genina, Artem A. Aratov,Anastasiya M. Parkheychuk, Valery V. Tuchin
Department of Optics and Biophotonics of Saratov State University
e-mail: [email protected]
Saratov State University
______________________________________________
Department of Optics & Biophotonics
__________________________________________________
Saratov Fall Meeting 2011
Motivation:Motivation:
It is well known that diagnostics and treatment of many diseases of human eye is connected with monitoring of glucose content. However, in spite of numerous investigations dealing with the transport of the metabolite within biological tissue, the problem of monitoring of structural changes of eye cornea under action of glucose has not been studied in detail.
Goal of the study is investigation of influence of 40% aqueous glucose solution on stuctural changes in human eye cornea at the noninflamation cornea edema after post-surgical phacoemulsification of aging cataract
It is well known that diagnostics and treatment of many diseases of human eye is connected with monitoring of glucose content. However, in spite of numerous investigations dealing with the transport of the metabolite within biological tissue, the problem of monitoring of structural changes of eye cornea under action of glucose has not been studied in detail.
Goal of the study is investigation of influence of 40% aqueous glucose solution on stuctural changes in human eye cornea at the noninflamation cornea edema after post-surgical phacoemulsification of aging cataract
Saratov State University
______________________________________________
Department of Optics & Biophotonics
__________________________________________________
Saratov Fall Meeting 2011
Materials and Methods: in vitro study
The study has been performed with ten samples of
rabbit cornea immersed in solutions with different pH in the
range from 3 to 9. The buffer solutions have been prepared
using sodium citrate (C6H5O7Na25*5H2O), citric acid
(C6H8O7*H2O), (Na2HPO4), (KH2PO4), sodium carbonate
(Na2CO3), and sodium bicarbonate (NaHCO3).
pH of these solutions (3.07, 4.04, 4.91, 5.5, 5.91, 7.01,
8.59, 8.99) have been measured by pH-meter "HANNA"
(Portugal) using standard procedure.
The study has been performed with ten samples of
rabbit cornea immersed in solutions with different pH in the
range from 3 to 9. The buffer solutions have been prepared
using sodium citrate (C6H5O7Na25*5H2O), citric acid
(C6H8O7*H2O), (Na2HPO4), (KH2PO4), sodium carbonate
(Na2CO3), and sodium bicarbonate (NaHCO3).
pH of these solutions (3.07, 4.04, 4.91, 5.5, 5.91, 7.01,
8.59, 8.99) have been measured by pH-meter "HANNA"
(Portugal) using standard procedure.
Saratov State University
______________________________________________
Department of Optics & Biophotonics
__________________________________________________
Saratov Fall Meeting 2011
Materials and Methods: in vitro study
For investigation of hydration degree, the samples have been
weighed using electronic balance (SCIENTECH, SA210) with the
precision of 1 mg. The tissue samples have been immersed into
cuvettes with the buffer solutions. Every 10 min during 2 hours, each
sample has been taken out from the solution and weighted. The
hydration degree of the cornea samples has been calculated using the
equation:
For investigation of hydration degree, the samples have been
weighed using electronic balance (SCIENTECH, SA210) with the
precision of 1 mg. The tissue samples have been immersed into
cuvettes with the buffer solutions. Every 10 min during 2 hours, each
sample has been taken out from the solution and weighted. The
hydration degree of the cornea samples has been calculated using the
equation:
where M is the mass of tissue sample and t is time. where M is the mass of tissue sample and t is time.
0,
0
M t M tH t
M t
Saratov State University
______________________________________________
Department of Optics & Biophotonics
__________________________________________________
Saratov Fall Meeting 2011
Materials and Methods: in vivo study
Cornea structure has been investigated using Heidelberg Retina Tomograph (HRT) with the Rostok Cornea Module (RCM) (Heidelberg Engineering GmbH, Germany)
Cornea structure has been investigated using Heidelberg Retina Tomograph (HRT) with the Rostok Cornea Module (RCM) (Heidelberg Engineering GmbH, Germany)
Saratov State University
______________________________________________
Department of Optics & Biophotonics
__________________________________________________
Saratov Fall Meeting 2011
Materials and Methods: in vivo study
Cornea thickness has been measured with Topcon 3D OCT-1000 Optical coherence Tomograph (OCT) (Japan)
Cornea thickness has been measured with Topcon 3D OCT-1000 Optical coherence Tomograph (OCT) (Japan)
Saratov State University
______________________________________________
Department of Optics & Biophotonics
__________________________________________________
Saratov Fall Meeting 2011
Materials and Methods: in vivo study
Sixty volunteers were enrolled for the study. The subjects were divided into two groups. The I group included thirty volunteers (21 male and 9 female at the age from 56 to 72 years) with post-surgical edema of cornea after phacoemulsification of aging cataract. The II (control) group included thirty health volunteers (16 male and 14 female at the age from 22 to 30 years).
For all persons the study of corneal structure and thickness was performed before the instillation of 40%-glucose solution (2 drops 0.05 ml = 0.1 ml) and in 20 and 40 minutes after the procedure with HRT and OCT
Sixty volunteers were enrolled for the study. The subjects were divided into two groups. The I group included thirty volunteers (21 male and 9 female at the age from 56 to 72 years) with post-surgical edema of cornea after phacoemulsification of aging cataract. The II (control) group included thirty health volunteers (16 male and 14 female at the age from 22 to 30 years).
For all persons the study of corneal structure and thickness was performed before the instillation of 40%-glucose solution (2 drops 0.05 ml = 0.1 ml) and in 20 and 40 minutes after the procedure with HRT and OCT
Saratov State University
______________________________________________
Department of Optics & Biophotonics
__________________________________________________
Saratov Fall Meeting 2011
Results: in vitro studyResults: in vitro study
3 4 5 6 7 8 9 10 11-0.2
0.0
0.2
0.4
0.6
0.8
1.0
1.2
Hyd
ratio
n de
gree
(H
)
pH
Cornea (t = 10 min) Cornea (t = 20 min) Cornea (t = 60 min) Gelatin hydration
H(t) = (M(t) - M(t = 0)) / M(t = 0)
Swelling
Shrinkage
Initial state
The comparison of hydration degree of rabbit cornea and collagen (gelatin) under action of solutions with different pH measured in different time. M is the mass of the sample; t is the time of action. The data about gelatin swelling have been obtained from Lykov A.V. Transport phenomena in capillaro-porous media / Moscow, 1954
Saratov State University
______________________________________________
Department of Optics & Biophotonics
__________________________________________________
Saratov Fall Meeting 2011
Results: in vivo studyResults: in vivo study
The typical OCT images obtained from cornea in normal state under action of 40%-glucose solution. (a) – before glucose instillation; (b) – after 20 min; (c) – after 40 min
a b
c
Saratov State University
______________________________________________
Department of Optics & Biophotonics
__________________________________________________
Saratov Fall Meeting 2011
Results: in vivo studyResults: in vivo study
The thickness of human cornea measured in vivo by OCT under action of 40%-glucose solution (pH = 3.5) in different time
0 20 40500
600
700
800
900
1000
1100
Thi
ckne
ss, m
Time, min
Cornea in normal state Cornea with edema
0 20 400.4
0.6
0.8
1.0
Hyd
ratio
n d
eg
ree
Time, min
The hydration degree of human cornea with edema measured in vivo under action of 40%-glucose solution (pH = 3.5) in different time
Saratov State University
______________________________________________
Department of Optics & Biophotonics
__________________________________________________
Saratov Fall Meeting 2011
Results: in vivo studyResults: in vivo study
The typical HRT images obtained from corneum epithelium in normal state under action of 40%-glucose solution. (a) – before glucose instillation; (b) – after 20 min; (c) – after 40 min
The typical HRT images obtained from corneum epithelium with edema under action of 40%-glucose solution. (a) – before glucose instillation; (b) – after 20 min; (c) – after 40 min
a b c
Saratov State University
______________________________________________
Department of Optics & Biophotonics
__________________________________________________
Saratov Fall Meeting 2011
Results: in vivo studyResults: in vivo study
The typical HRT images obtained from corneum endothelium in normal state under action of 40%-glucose solution. (a) – before glucose instillation; (b) – after 40 min
The typical HRT images obtained from corneum endothelium with edema under action of 40%-glucose solution. (a) – before glucose instillation; (b) – after 40 min
a b
Saratov State University
______________________________________________
Department of Optics & Biophotonics
__________________________________________________
Saratov Fall Meeting 2011
Results: in vivo studyResults: in vivo study
0 20 400
2
4
6
8
10
12
Dia
me
ter,
m
Time, min
Cornea in normal state Cornea with edema
The diameter of the corneal epithelial cells measured in vivo by HRT under action of 40%-glucose solution in different time
The diameter of the corneal endothelium cells measured in vivo by HRT under action of 40%-glucose solution in different time
0 20 40 600
4
8
12
16
20
24
Dia
met
er, m
Time, min
Cornea in normal state Cornea with edema
Saratov State University
______________________________________________
Department of Optics & Biophotonics
__________________________________________________
Saratov Fall Meeting 2011
Russian Federation governmental contacts 02.740.11.0770, and 02.740.11.0879
Russian Federation governmental contacts 02.740.11.0770, and 02.740.11.0879
Grant #224014 Network of Excellence for Biophotonics (PHOTONICS4LIFE) of the Seventh Framework Programme of Commission of the European Communities
Grant #224014 Network of Excellence for Biophotonics (PHOTONICS4LIFE) of the Seventh Framework Programme of Commission of the European Communities
Acknowledgement:
The authors thank Dr. Alexander B. Pravdin (Department of Optics and Biophotonics of Saratov State University) for the help in preparation of the buffer solutions