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Opportunity overview with OriGene Technologies. OriGene is looking to identify strategic collaboration partners for its full-length human proteins, validated monoclonal antibodies and "gene centric" tool box.
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Developing Better Assays for Every Human Protein
OriGene Technologies, Inc.
Mark E. WatsonSr. Director Business Development
Phone: (240)994-6873Email: [email protected]
Collaboration Opportunities with OriGene
• Strategic Licensing Agreements
• Supply agreement for cDNA clones, full-length human proteins and validated monoclonal antibodies
-Purchase of full cDNA clone collection or subsets-Human and Mouse ORF clones available -Bulk full-length protein development -Licensing of hybridoma cell lines (e.g. TrueMAB mAbs)-Joint mAb and/or Hybridoma cell line development -Custom multiplex assay development
• Collaboration agreements for antibody decoding and auto-antibody studies using the proprietary protein lysate microarray technology from OriGene
• Custom service projects leveraging gene synthesis capabilities from Blue Heron Biotechnology, including codon & antibody optimization
OriGene’s Key Capabilities• Largest collection of cDNA clones in the world • Protein production in human cells
– 12,000 transfected antigen lysates– 5,000 purified proteins from HEK293Tcells– Est.10,000 available by the end of 2011– Heavy-labeled MRM/Mass spec. standards.
• High throughput mAb production/ validation – Recently opened second facility to expand production to over 500 released and fully
validated mAbs/month– Three fold increase in production capabilities by year end 2011
• Cytomyx tissue bank: – >140,000 tissue samples – High throughput IHC validation capabilities
• Synthetic Gene Production & Services– Blue Heron Biotechnology Acquisition (Ab & Codon optimization)
• 10,000 Antigen Chip (Autoimmune & Cancer tests)• Luminex multiplex assay development (Marligen acquisition)
Complete Gene/clone Resource
Clones/genes Proteins Antibodies Assays
TrueORF/GoldHuman/mouseExpression vectors
True Proteins5,000 humanCustom purification
TrueMAB>1,800 mAbsIHC, IF, IP, FC, WB
TruePlex
Any speciesAny sequencesAny variants
Optimized expression
Antibody engineering Multiplex Assays
High Throughput Work Flow
15,000TrueORF Clones
12,000Over-expression Lysates
5,000Purified Protein
Transient Transfection(MegaTran)
Cell Lysates AffinityColumn
Largest offering of mammalian cell expressed human proteins
Highest Quality Standard
Not just sequence-verification
But also,
Expression Validation on 12,000 TrueORF clones
APO3C
TrueORF:RC206566
ORF size: 300 bp
Predicted MW: 11 kd
TP53
TrueORF:RC200003
ORF size: 1182 bp
Predicted MW: 53 kd
ENC1
TrueORF:RC201842
ORF size: 1770 bp
Predicted MW: 65 kd
LRP1
TrueORF:RC218369
ORF size: 13635 bp
Predicted MW: 504 kd
CDH3
TrueORF:RC207346
ORF size: 2490 bp
Predicted MW: 91 kd
Transfected Over-Expression Lysates
• 12,000 TrueORF clone transfected HEK293T cell lysates
• Mammalian cell expressed full length, WB validated
• Delivered in modified RIPA buffer to preserve protein function
• Ideal tools for antibody validation, protein function studies, and as assay standards
Over-expression Lysates
• OriGene over-expression lysates adopted in Antibody Atlas Program to validate polyclonal antibodies (e.g. Atlas Antibodies).
• Improved validation success rate from 32% with regular cell lysates to 89% with over-expression lysates.
• Data from Prof Mathias Uhlén, Program Director.
Using OriGene antigen microarray chip technology to decode antibodies that were generated by whole cell immunization
Anti-DDK (Rabbit) Anti-E-Cadherin1 (mAb)
Enlarged image
E-Cadherin1
10,000 Lysate Protein Arrays
Full Length Human Proteins
• Produced from TrueORF cDNA clones
• 5,000 full length human proteins
• Expressed in HEK293 cells
• Optimal preservation of protein structure, post-translational modifications and functions
Human Protein Activity
mTOR Activity Assay
Homogeneous time-resolved fluorescent (HTRF®) assay.
mTOR is a serine/threonine protein kinase that regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription. mTOR belongs to the phosphatidylinositol 3-kinase-related kinase protein family.
First commercial provider of active, full-length human mTOR protein
MS Collaboration with ISB
• Generate signature peptide sequences for all 5,000 human proteins – Sequences will be in PeptideAtlas database
• Heavy-isotope labeled purified proteins– 5,000 available now for SRM/MRM application
• Isotope labeled peptides –Coming in 2011 for all 5,000 human protein
mAbs for Every Human Protein
Dedicated mAb production center - Capacity: +200 immunizations per month
Authentic proteins as antigens- Full length proteins made in human cells
Extensive validation- WB: 9 different cell lines- IHC: 24 different tissues - IF- Flow cytometry- Luminex- Additional validation available
Hybridoma cell lines for licensing- High value targets available: ERCC1, LGR5,
HES1 & NOTCH1
Goal: To produce high quality mAbs to every human proteins in 5-10years
HTP Validation of mAbs
IHC data for human carcinoma liver tissue
Anti-KRT WB with OriGene over expression lysate
Anti-KRT WB with cell panel of 9 different cell lines(For endogenous protein detection in cell line)
ERCC1 (4F9)ERCC1 (1A3)IF data with A549 cells IF data with A549 cells
0
5000
10000
15000
20000
25000
30000
1 10 100 1000 10000
Analyte Concentration (pg/ml)
FGF -4
VEGF -D
EGF
HGF
PlGF
VEGF -A
Med
ian
Fluo
resc
ent I
nten
sity
Quantitation of 6 Growth Factors in an 11-plex ELISA
Immunoassay Development
Summary
OriGene has developed the most comprehensive genome wide product solutions available
Leading developer of high quality reagents and multiplex assays for every human protein
OriGene is your perfect partner for companion diagnostic and biomarker assay development
Additional information available at:
www.origene.com