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Vocabulary Review
• What is an autotroph?– Make their own energy
• What is a heterotroph?– Aren’t able to make own energy– have to get it
from another source
Photosynthetic Organisms
• Photosynthesis is one way to make your own energy
• Who does it?– Plants, algae, some other
protists, some prokaryotes (ex. cyanobacteria)
Location of Photosynthesis
• Photosynthesis occurs in the chloroplasts – Organelles that contain the
pigment chlorophyll and other pigments
– Openings in the leaf (stomata) allow CO2 (reactant) to enter and O2 (product) to leave
Equation for Photosynthesis
6 CO2 + 6 H2O + ENERGY C6H12O6 +
6 O2
• In terms of reactants and products, it is the reverse of cellular respiration, but process is different.
Stages of Photosynthesis
• Light Reactions– Captures light energy to
produce ATP and NADPH
• Calvin Cycle (“Dark” Reactions)– Uses ATP and NADPH to
“fix” carbon into a carbohydrate
– “fix” means to incorporate the carbon into a compound
Light Reactions• Main point = convert sunlight
energy into chemical energy (ATP and NADPH)
• Sunlight can be divided into different wavelengths of light– only some are useful in photosynthesis
Light Reactions
• Photosynthetic pigments in the chloroplast absorb different wavelengths – Chlorophyll a– Chlorophyll b– Carotenoids
Question…
• Why are most plants green?
• The chlorophyll and carotenoid molecules absorb the blue-violet and red light and use it to perform photosynthesis
• They reflect green light – colors we see are the reflected wavelengths.
Light Reactions: Photosystems
• Photosystems are the complex of pigments that receives the sunlight to begin the process
• Photosystems absorb energy to excite an electron in chlorophyll
• There are 2 different photosystems in chlorophyll – I and II.
Light Reactions: Noncyclic Flow
• Step 1: Light excites electrons in photosystems I and II
• Step 2: Excited electrons are passed down an electron transport chain – generates ATP and NADPH
Noncyclic Flow
• Excited electron from Photosystem II fills the space left in Photosystem I
• Water is split (photolysis) to provide an electron to fill the space left in Photosystem II
Cyclic Electron Flow
• Under certain circumstances, the excited electrons will only flow through Photosystem I (not II)
Cyclic Electron Flow
• Generates ATP
• Doesn’t generate NADPH
• Runs because the Calvin Cycle needs more ATP than NADPH
• May shift from noncyclic to cyclic flow when NADPH levels are high
Electron Transport Chain
• very similar to cellular respiration
• An H+ concentration gradient across the thylakoid membrane drives ATP synthesis
Calvin Cycle• Also called the Dark Reactions, Non-light
Dependent Reactions, or the Calvin Benson Cycle
• Uses the ATP and NADPH produced in the light reactions to produce glucose using CO2
Calvin Cycle: Rubisco
• Rubisco is the enzyme that first “traps” CO2 into the cycle
• Through a series of reactions, CO2 is used to eventually make glucose
Calvin Cycle
• CO2 only contributes 1 carbon 6 “turns” of the calvin cycle are needed to produce one glucose.
• RuBP also has to be regenerated so that cycle can continue
The O2 released during photosynthesis comes from
CO2
H2O
NADPH
RuBP (R
uDP)
C6H
12O
6
0% 0% 0%0%0%
1. CO2
2. H2O
3. NADPH
4. RuBP (RuDP)
5. C6H12O6
15
Carbohydrate-synthesizing reactions of photosynthesis directly require
Lig
ht
Pro
ducts
of the
light
...
Dar
knes
s
O2
and H
2O
Chlo
rophy
ll an
d CO2
0% 0% 0%0%0%
1. Light
2. Products of the light reactions
3. Darkness
4. O2 and H2O
5. Chlorophyll and CO2
15