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PhotosynthesisThe process autotrophs use to
make glucose sugars from carbon sources, water, and light energy
6CO2 + 6H2O C6H12O6 + 6O2
sunlight
Radiant energycarbohydrates
Respiration
How energy flows through the ecosystem
Photosynthesis
HeterotrophsHeterotrophs
Autotrophs
heat
How organisms get carbon energy
1. Autotrophs: able to produce own glucose – Ex: plants, algae, cyanobacteria– Also called: producers
2. Heterotrophs: must take in glucose from outside source
– Ex: animals, fungus, most bacteria, protozoans
– Also called: consumers
RespirationPhotosynthesis
Stored Chemical Energy(in the form of Glucose)
Light Energyfrom the sun
Energy for Cellin the form of ATP
Using oxygen; releasing w
ater & CO
2
Usi
ng w
ater
& C
O 2; re
leas
ing
oxyg
en
Why is a carbon source from sugars so important?• The carbons are needed to make ATP
molecules, which provide energy for metabolic reactions.
Adenine Ribose 3 Phosphate groups
ADP and ATP
• To get energy out of ATP, the bond between two phosphate groups is broken.
ADP ATP
Energy
EnergyAdenosine diphosphate (ADP) + Phosphate Adenosine triphosphate (ATP)
Partiallychargedbattery
Fullychargedbattery
Where and how are sugars made?Light Energy
Chloroplast
CO2 + H2O Sugars + O2
Photosynthesis video
Click on image to play video.
Pigments
• Chlorophyll is a pigment, a molecule that can absorb light energy.
• Unused light is reflected.
• What is the color of the wavelength least used by chlorophyll?
Absorption of Light byChlorophyll a and Chlorophyll b
V B G YO R
Chlorophyll b
Chlorophyll a
2. Photosynthesis pigments
• A. chlorophyll a(light green)
• B. chlorophyll b(dark green)
• C. xanthophyll(yellow)
• D. carotenoid– (orange)
Chromatography
• The process of separating colored solutions to determine the number of pigments in the solution
How does photosynthesis work?
1. Light dependent reaction
2. Calvin cycle
ChloroplastLight
Sugars
CO2
Light-Dependent Reactions
CalvinCycle
NADPH
ATP
ADP + PNADP+
Chloroplast
H20
O2
Step 1: Light dependent reaction
HydrogenIon Movement
Photosystem II
InnerThylakoidSpace
ThylakoidMembrane
Stroma
ATP synthase
Electron Transport Chain Photosystem I ATP Formation
Chloroplast
Light dependent reaction
• Pigments (chlorophyll) inside of the chloroplasts are arranged into photosystems (PSI and PSII).
• Photosystems absorb sunlight.
• Electrons become energized and help to produce NADPH.
3. Light Dependent
reactions• location:
grana of chloroplast• Photosystem II: energized chlorophyll releases
energy to split water into Oxygen (released) and Hydrogen (the H is carried by NADP to be used later in Calvin cycle)
• Photosystem I: energized chlorophyll releases energy to add phosphate to ADP to make ATP (to be used later in the Calvin cycle)
Light dependent video (1)
Click on image to play video.
Light dependent video (2)
Click on image to play video.
Step 2: Calvin cycle
ChloropIast
CO2 Enters the Cycle
Energy Input
5-CarbonMoleculesRegenerated
Sugars and other compounds
6-Carbon SugarProduced
4. Light independent reactions(Calvin cycle)
• Location: stroma (fluid) of chloroplast
• Carbons? CO2 is “fixed” by RuBP & begins the cycle becoming PGA, then PGAL after hydrogen (from NADP) and energy (from ATP) are added
• Products? RuBP is recycled for next time & Glucose (sugar/food) is made (from 6 turns of cycle)
Calvin cycle
• The NADPH supplies the energy needed to change the CO2 taken into the cell into a 6 carbon molecule.
• This 6 carbon molecule is made into sugars.
glucose
Sunlight
Light-dependentReactions
water
oxygen
Calvin Cycle
Carbon Dioxide Glucose
NADPH2
ATP
Within chloroplasts
RuBP
PGA
PGAL
Calvin cycle video
Click on image to play video.