Pulse Electroporation System

Onyx Medical Inc.

Various TDD Technologies Various TDD Technologies ElectroporationElectroporation - - Using short electrical modulated pulses of Using short electrical modulated pulses of

high voltage to create transient aqueous pores in the skin.high voltage to create transient aqueous pores in the skin. IontophoresisIontophoresis – Using low voltage electrical current to drive – Using low voltage electrical current to drive

charged drugs through the skin.charged drugs through the skin. SonophoresisSonophoresis – Using ultrasound to disrupt the stratum – Using ultrasound to disrupt the stratum

corneum. corneum. ThermalThermal energyenergy – Using heat to make the skin more permeable – Using heat to make the skin more permeable MagnetophoresisMagnetophoresis - has been investigated as a means to - has been investigated as a means to

increase drug flux across the skin.increase drug flux across the skin. Needle Free InjectionNeedle Free Injection - devices for “firing” molecules through - devices for “firing” molecules through

the skin at high velocitythe skin at high velocity

ElectroporationElectroporation

Non-invasiveNon-invasive Alternative to mesotherapyAlternative to mesotherapy Virtual mesotherapy or Virtual mesotherapy or

without needles without needles Painless procedurePainless procedure Introduction of drugsIntroduction of drugs alternating currentalternating current rearrange the structure in the rearrange the structure in the

cells of the outer layer of skincells of the outer layer of skin Acts as a virtual syringeActs as a virtual syringe

Changes direction (polarity) Changes direction (polarity) continuously slowlycontinuously slowly

Generating new poresGenerating new pores Micro and macromoleculesMicro and macromolecules 500,000 Dalton as collagen.500,000 Dalton as collagen. 800.000 Dalton as hyaluronic 800.000 Dalton as hyaluronic

acidacid

ElectroporationElectroporation

For electroporation we need:For electroporation we need: Membrane potential: 0.5-1.5 voltsMembrane potential: 0.5-1.5 volts Disrupt lipid layerDisrupt lipid layer Open biological channels (aquous)Open biological channels (aquous) These channels stay open several secondsThese channels stay open several seconds Change polarity constantlyChange polarity constantly

When the pulse ceases, the membrane returns to its When the pulse ceases, the membrane returns to its original structure, leaving retained product that has original structure, leaving retained product that has

penetrated into the interior of the cell in place. penetrated into the interior of the cell in place.

The application of an electrical pulse momentarily disrupts the cell membrane, allowing entry of

product.

Background

ElectroporationElectroporation

Electric doors called Electric doors called biological channelsbiological channels

Electric pulse changes Electric pulse changes membrane polaritymembrane polarity

By slowly changing the By slowly changing the polarization we do not polarization we do not cause electrolysiscause electrolysis

This disrupts lipid layerThis disrupts lipid layer

Open ChannelsOpen Channels

Intercellular CommunicationsIntercellular Communications

Cellular PenetrationCellular Penetration

ElectroporationElectroporation

This effect lasts for seconds, therefore allowing the introduction of This effect lasts for seconds, therefore allowing the introduction of substances.substances.

Hydrofilic and hydrophobic molecules penetrate at the rate of Hydrofilic and hydrophobic molecules penetrate at the rate of 1 g every 1 - 1.5 minutes1 g every 1 - 1.5 minutes..

Continuous formation and appearance of biological channelsContinuous formation and appearance of biological channels Dramatically increases skin permeabilityDramatically increases skin permeability..

ConclusionConclusion

Electroporation is a process that uses high-voltage current to Electroporation is a process that uses high-voltage current to make cell membranes permeable to allow the introduction of make cell membranes permeable to allow the introduction of new materials.new materials.

In a practical sense Electroporation is opening a door that In a practical sense Electroporation is opening a door that

allows us to insert “products” into the bodyallows us to insert “products” into the body..

MethodologyMethodology

Research documents 4 times Research documents 4 times better results when you do an better results when you do an exfoliation such as a peel or exfoliation such as a peel or micromicrodermabrasion micromicrodermabrasion before Electroporation.before Electroporation.

This eliminates dead cells This eliminates dead cells from the Keratinic layer.from the Keratinic layer.

This changes the skin This changes the skin potential and makes it easier potential and makes it easier to create biological channels.to create biological channels.

Comparison with MicrodermabrationComparison with Microdermabration

IndicationsIndications

Introduction of substances for:Introduction of substances for:– Skin treatmentSkin treatment– CelluliteCellulite– FlaccidityFlaccidity– WrinklesWrinkles– ObesityObesity– Collagen productionCollagen production

ContraindicacionesContraindicaciones

Pacemakers, heart valves Pacemakers, heart valves or other active implants. or other active implants.

EpilepsyEpilepsy Pregnant womenPregnant women Immediately after Botox Skin outbreaks or on Skin outbreaks or on

infected tissueinfected tissue Metal plates or other Metal plates or other

metal objectsmetal objects Ulcer or gastroenteritisUlcer or gastroenteritis

Wrinkles

Instruction:Clean and dry skin the skin. Exfoliate by microdermabrasion. Optional: Use a micro roller after microderm.

Select Protocol

Power:70% - 80%Time of application: 20 – 25 minutes

Amount of product:4 to 6 cc, divided into face, neck, and décolleté

Method:Circular motion, soft and slow until completely absorbed

Frequency of session:One session a week during five to six weeks After one session every two week for one month A monthly session as maintenance

Decolette

Instruction:Clean and dry skin the skin. Exfoliate by microdermabrasion. Optional: Use a micro roller after microderm.

Select Protocol

Power: 70% - 80% Time of application : 15 – 20 minutes

Amount of product :4 cc

Method:Circular motion, soft and slow until completely absorbed

Frequency of session:One session a week during five to six weeks After one session every two week for one month A monthly session as maintenance

Body Firming

Instruction:Clean and dry skin the skin. Exfoliate by microdermabrasion. Optional: Use a micro roller after microderm.

Select Protocol

Power: 80% - 90%Time of application: 15 – 20 minutes

Amount of product: 5 to 6 cc (depending if we have to reduce or reaffirm)

Method:Circular motion, soft and slow until completely absorbed

Frequency of session: One session a week during six to eight weeks After one session every two week for one month A monthly session as maintenance

Breast Firming

Instruction:Clean and dry skin the skin. Exfoliate by microdermabrasion or gentle enzyme peel.

Select Protocol

Power: 80% - 90%Time of application: 15 – 20 minutes

Amount of product: 3 - 4 cc

Method:Circular motion, soft and slow until completely absorbed

Frequency of session: One session a week during six to eight weeks After one session every two week for one month A monthly session as maintenance

Facial Rejuvenation

Instruction:Clean and dry skin the skin. Exfoliate by microdermabrasion. Optional: Use a micro roller after microderm.

Select Protocol

Power: 70% + Face, 30-40% LipsTime of application: 20 – 25 minutes

Amount of product: 5 to 6 cc, divided into face, neck, and chest (also hands)

Method:Circular motion, soft and slow until absorbed

Frequency of session:One session a week for three to four weeks

After one session every two week for one monthA monthly session as maintenance

Localized Obesity

Instruction:Clean and dry skin the skin. Exfoliate by microdermabrasion. Optional: Use a micro roller after microderm.

Select Protocol

Power: 85% - 95%Time of application: 15 – 20 minutes

Amount of product: 6cc - 10 cc

Method:Circular motion, soft and slow until absorbed

Frequency of session: One session a week during six to eight weeks After one session every two week for one month A monthly session as maintenance

Cellulite

Instruction:Clean and dry skin the skin. Exfoliate by microdermabrasion. Optional: Use a micro roller after microderm.

Select Protocol

Power: 85% - 95%Time of application: 15 – 20 minutes

Amount of product: 6 - 10 cc

Method:Circular motion, soft and slow until absorbed

Frequency of session: One session a week during six to eight weeks After one session every two week for one month A monthly session as maintenance

ELECTROPORATIONELECTROPORATION

ONYX MEDICAL INC.ONYX MEDICAL INC.

1-800-333-57731-800-333-5773

WWW.ONYXMEDICAL.COMWWW.ONYXMEDICAL.COM