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Catalog No. M-0300

R & F® Escherichia coli O157:H7 Chromogenic Plating Medium

A Differential/Selective Chromogenic Plating Medium For The Rapid Identification And Isolation Of Presumptively Positive Colonies Of Escherichia coli O157:H7

Presumptively positive colonies of Escherichia coli O157:H7 appear as blue-black domed colonies 1.5 to 2.5 mm in diameter with a

black precipitate after 20-24 hours incubation at 35°C.

U.S. Patent 6,087,156 and 6,617,149European Patent 1,045,923

LISTED IN FDA’s BAM

R & F® Escherichia coli O157:H7 Chromogenic Plating Medium at 35°Cfor 20-24 hours

Advantages of R & F® Escherichia coli O157:H7 Chromogenic Plating Medium

• AselectiveplatingmediumthatdifferentiatesEscherichia coli O157:H7 from most other strains of Escherichia coli

• Differentialcharacteristicsbasedonpositivechemical attributes of Escherichia coli O157:H7

• Isolatesβ-glucuronidasepositiveandslowfermentingsorbitolpositiveEscherichia coli O157:H7 variants

• Simplepreparationofplatesthatremainstablefor at least 45 days stored in the dark at 2-8°C

• Differentiationbasedonβ-galactosidasereactionand 4 carbohydrate fermentations coupled with a pH indicator

References

Onoue,Y.,H.Konuma,H.Nakagawa,Y.Hara-kudo,T.Fujita,andS.Kumagai.1999.CollaborativeevaluationofdetectionmethodsforEsch-erichia coli O157:H7fromradishsproutsandgroundbeef.International Journal of Food Microbiology 46: 27-36.

Reissbrodt,R.1998.EnterohemorrhagicEscherichia coli O157:H7 isolation and identification. Biotest Bulletin 6: 65-74.

Restaino,L.1996.Accentuatethepositive.Food Quality(USA)Nov./Dec.:68-70.

Restaino,L.,E.W.Frampton,K.N.Turner,andD.R.K.Allison.1999.AchromogenicplatingmediumforisolatingEscherichia coli O157:H7 from beef. Letters in Applied Microbiology.29:26-30.

ORDERING INFORMATION:

M-0300 R & F® Escherichia coli O157: H7Chromogenic Plating Medium

M-0310 R & F® Escherichia coli O157: H7Supplement for Plating Medium

M-0350 R & F® Escherichia coli O157: H7Chromogenic Detection System

BacteriaNumber

ofStrains

Colonial Morphology

Escherichia coliO157:H7Typicalstrains

Escherichia coliO157:H7 β-glu-curonidase positive

Escherichia coliO157:H7 sorbitol positive

38

1

1

Domedtoraisedcolony,1.5-2.0 mm diameter, dark blue to black, with a black precipitate

Domedtoraisedcolony,2.0mm diameter, dark blue to black, with a blackprecipitate

Domedtoraisedcolony,2.0 mm diameter, dark blue to turquoise, with a black precipitate

Escherichia coli O26 3 Domedcolony,greenwithbrownish center

Escherichia coli O111 2 Domedcolony,greenwithbrownish center

Escherichia hermanii 2Domed;pinpointto<1mmdiameter,cleartolightbluecolor

Escherichia coli(generic) 11

3strainsnogrowth,pinpointto 2 mm diameter, clear to green

Salmonella sp. 5 minimalgrowth<1to1mmdiameter, clear color

Pseudomonas sp. 3 Domed;pinpointto1to2mm diameter, clear color

Providencia stuartii 1 Domed;pinpointto<1mmdiameter, clear to white color

Klebsiella sp.Enterobacter sp.Proteus sp.Morganella sp.Citrobacter sp.Acinetobacter calco-aceticusProvidencia alcafaciensYersinia enterocolitica

15 Nogrowthforallstrainstested

R&FProducts,Inc.2725CurtissSt.DownersGrove,IL60515

Phone:630.969.5300Fax:630.969.5303E-mail:rf@rf-products.netWebSite:www.rf-products.net