Welcome

Seminar: Opportunities for studying cellular metabolism in live cells with the Seahorse analyzer 12 April 2017

Programme today

9.30 – 12.00

Seminar “Measuring mitochondria and metabolism with the Seahorse – New opportunities and applications”

13.00 – 17.00

Seahorse demonstration and training workshop

Introduction Shared Research Facilities and today’s seminar

Petra Caessens, Manager Operations Shared Research Facilities

Wageningen, 12 April 2017

Shared Research Facilities in practice For researchers from in and outside WUR

Video

Wageningen University & Research, Shared Research Facilities

Investments in and providing access to advanced research equipment Organise facility-sharing WUR / Campus

Organise seminars to stimulate facility sharing

Studying live cells – glimpse of facilities

Seahorse: analyzing cellular metabolism

Live cells

Laser microdissetion: dissection of cells

from tissues

BD Pathway: screening of cellular behaviour of a

large numbers of compounds

Light microscopes (CSLM and spinning disk): imaging cells

FACS flow cytometer and cell sorter: sorting and

analyzing cells

Programme today

9.30 – 12.00

Seminar “Measuring mitochondria and metabolism with the Seahorse – New opportunities and applications”

13.00 – 17.00

Seahorse demonstration and training workshop

Seminar programme

9:30 Petra Caessens (WUR Shared Research Facilities) 9:35 Daniel Gebhard (Agilent Technologies) "Introduction to the Seahorse technology” & "Bringing metabolomic pathways to life with real-time measures of cellular energy metabolism” 10:15 Jan van den Bossche (Department of Medical Biochemistry, Experimental Vascular Biology, Academic Medical Center, Amsterdam) "Mitochondrial Dysfunction Prevents Repolarization of Inflammatory Macrophages” 11:00 Break 11:15 Jaap Keijer (Human and Animal Physiology - WUR) "Challenging metabolism by limiting oxygen” 12.00 End of the seminar

Contact us

Shared Research Facilities www.wur.eu/shared-research-facilities