Shop! - es.vwr-cmd.comes.vwr-cmd.com/.../biomarke/0711/BioMarkeShop9_ES.pdf · performance. all cyclers contain the active Sample Cooling (aSC) function that cools your sample until

http://es.vwr.com

Promotions for Life Scientists Issue 9 - November 2007EditorialShop!

Molecular biologyThe new generation thermal cycler reliable and reproducible thermal performance characteristics are essential elements of a good thermal cycler and the VWr Collection UnoCycler delivers every time. Each thermal block has 4 independent temperature control sensors and 8 peltier heating units that ensure accurate heating and cooling control, unsurpassed uniformity across the block surface and superb thermal cycling performance. all cyclers contain the active Sample Cooling (aSC) function that cools your sample until the heated lid reaches its target temperature. this feature reduces non-specific primer binding and extension. all instruments have a 2-year warranty.

Buy the UnoCycler at a very special price today and choose between standard and fast blocks:• ramp rate of up to 3 °Cs-1 with standard blocks• ramp rate of up to 6 °Cs-1 with fast blocks (Fast blocks give increased productivity and faster protocols)

EditorVWR International Europe bvbaHaasrode Researchpark Zone 3Geldenaaksebaan 4643001 LeuvenBelgium

CopywritingVWR International

Layout and typesettingMarketing Services VWR

PrintingStork, GermanyNo part of this publication may be reproduced or copied without prior permission by writing of VWR International Europe.

Run76.500 copiesPublication date: November 2007Due to the high sales volume of promoted articles someitems may be temporarily out of stock - VWR Terms andConditions of Sale apply.

Issue 9, November 2007 • vALID uNTIL 31sT mArch 2008 • check ouT www.vwr.com for fuLL specIfIcATIoNs

www.vwr.com

Molecular biology• VWR Collection Unocycler and gel

documentation system• DNA extraction kits from 5 PRIME• CBS PCR workstations• BTX HT Multi-Well Electroporation • Axygen plates for Real-Time PCR and

sequencing• RNase AWAY® or DNA AWAY® Wipes• Thermo Scientific Taq Polymerase

Cell Biology• Optimise your immunoassays• Nunc OptiCell®

• VWR Collection tube rotator• Microplate readers and washers from

Thermo Scientific

Proteomics• ProteoExtract® Subcellular Proteome

Extraction Kit from Merck Biosciences• Pall Acrosep Chromatography columns• Whatman FAST® slides for protein

microarray

Lab supplies• New BD Falcon™ flip top tubes • Brand Transferpette S multichannel• Nalgene tank Liners• Spectrum Mediakap

Biosciences• Thermo Biopolymers ProjectPeptides™• BioGenes monoclonals• Biosirius Protein Investigator

promo code1

promo code1

Unocycler Cat. No. Price Promo Price

Without block 732-1200P 5380,00€ N/aWith standard gradient block, 96 well 732-9986P N/a 5140,00€With fast gradient block, 96 well 732-9987P N/a 6920,00€With standard gradient block, 384 well 732-9988P N/a 5430,00€With standard gradient block, 384 well 732-9989P N/a 6570,00€Separate blocks:

96-well gradient block 732-1203P 2380,00€ 1904,00€96 well fast gradient block 732-1205P 4110,00€ 3288,00€384 well standard gradient block 732-1204P 2810,00€ 2410,00€384 well fast gradient block 732-1206P 3632,00€ 3900,00€

Buy one get one free*

It’s time for highly pure genomic DNA from any sample sourceChoose from 5 PRIME’s exciting genomic DNA purification tools to find the optimal solution ArchivePure DNA Purification System • Highly flexible and scalable liquid-based method for archive-quality dNa from a multitude of sample typesPerfectPure DNA Purification Kits • Fast and efficient column-based dNa purification from blood and cells without overloading and cloggingReady PCR DNA Purification Kits • Very fast and unique filtration method to generate PCr-ready dNa for screening

Gel electrophoresis of genomic dNa purified with the archivePure dNa Purification System

*identical kits

promo code3

Welcome to the last edition of the VWrbioMarke Shop for 2007. as preparations will shortly begin for the Christmas and New Year celebrations now is the time to give your work a treat with a choice of some excellent offers in molecular biology, cell biology and more. . .

We hope that you have an excellent finish to 2007 and enjoy our companion journal the VWrbioMarke magazine packed full of technical data and applications.

Here’s to a healthy, happy and successful 2008!

Your VWr bioMarke team

For high throughput requirements, the duo and QuattroCycler have been developed with two or four fully independent thermal bays and fully automated heated lids, please ask for details.

Special introductory offer on the UnoCycler

Great value

Contact your local VWr sales representative for full view of the benefits this new range of

instruments will provide.

ask for a demo or visit vwr.com for demo software download.

BTX HT Multi-Well Electroporation – Transfections in seconds…HT Electroporation SystemsBtX Ht systems allow you to boost productivity by optimising both electrical and biological conditions associated with electroporation, increasing yields and viability. Now entire libraries can be screened rapidly and efficiently, saving time and money.

promo code2

Model Generator Plates Plate handler Cat. No. Price Promo Price

Ht 25/200 830 ECM™ 830 6 x 25 Well EP Ht-200 732-0342P 13050,00€ 11745,00€Ht 96/200 830 ECM™ 830 2 x 96 Well EP Ht-200 732-0344P 13050,00€ 11745,00€Ht 25/100 630 ECM™ 630 6 x 25 Well EP Ht-100 732-0472P 8890,00€ 8001,00€Ht 96/100 630 ECM™ 630 2 x 96 Well EP Ht-100 732-0474P 9160,00€ 8244,00€

Save 1000€ on the purchase of a BTX

HT system

The BTX HT systems feature• 25 or 96 samples transfected in

just seconds• Multiple experiments in the

sample plate

Applications include• transfection of mammalian cells• transformation of bacteria and

yeast• cdNa library screening• drug screening

iN tHiS iSSUE

Genomic DNA from Product Size Cat. No. Price

Whole blood, bone marrow, packed cells and buffy coat

archivePure dNa Blood Kit 3 ml 733-1013P 21,80€

Cells and fresh, frozen, fixed or paraffin-embedded tissue

archivePure dNa Cell/tissue KitarchivePure dNa tissue Kit

100 mg100 mg

733-1017P733-1021P

21,80€ 21,80€

Mouse tails archivePure dNa Mouse tail Kit 100 mg 733-1024P 21,80€Buccal cells archivePure dNa Buccal Kit 10 Preps 733-1027P 21,80€Plant tissue archivePure dNa Plant Kit 200 mg 733-1031P 21,80€Yeast & Gram-positive bacteria archivePure dNa Yeast & Gram + Starter Kit 733-1032P 21,80€

PerfectPure DNA Purification Kits

Whole blood, buffy coats PerfectPure dNa Blood Kit 50 Preps 733-1068P 74,00€Cultured cells PerfectPure Cultured Cell Kit 50 Preps 733-1071P 161,00€

Ready PCR DNA Purification Kits

Whole blood, buffy coat, plasma, body fluids and cultured cells

ready PCr dNa Column Kit - Purification on a filter-based column

10 Preps 733-1049P 21,80€

ready PCr dNa Plate Kit – Purification on a filter-based 96 well plate

3 Preps 733-1056P 522,00€

ready PCr dNa disk Kit – Purification on sample collection cards

10 Preps 733-1053P 21,80€

Have you got your copy of the latest VWRbioMarke magazine?Full of technical and application articlesSee www.vwr.com

Molecular biology

ISSuE 9 - NOVEMBER 2007Shop!promo code

6promo code

4 Plates and Mats for Real-Time PCR and Sequencing• Plate wells have ultra-thin, consistent wall thickness giving precise thermal transfer

along entire surface of each well• all lots undergo the most rigorous Qa/QC procedures in the industry• Ensures consistent, reliable contamination-free performance• Plates available to fit most popular thermal cyclers• axyMats - re-usable to help reduce costs

Get 25% discount on accessories (mats and film)

Place your order today! Visit our website http://es.vwr.comLocal price agreements apply.

offers valid from 19th November 2007 to 31st March 2008Promotions in the VWrbioMarke SHoP may not be used in combination, VWr reserves the right to refuse invalid orders.

2

Order 5 packs but pay for only 4

The VWR Collection introduces a new, complete range of gel documentation systemsFeaturing the GenoSmart darkroom that allows the use of many bench top UV transilluminators - even one you might currently have in your laboratory. the system is compatible with most commonly used electrophoresis gels stains and by using a white or blue light converter you can also capture images from other applications such

as petri dishes, cells in flasks, autoradiographs, membranes, spot and slot blots of dNa, rNa or protein, tlC plates and more.

an easy-to-use “Stand alone”- instrument for fast documentation and analysis of dNa and protein gels. data can be transferred to PC via USB stick, leaving the unit free for the next user, whilst you work with your images.• real-time images from 8-bit CCd camera, (768 x 582 pixel resolution)• Self-contained, microprocessor controlled darkroom• integrated colour lCd screen for image viewing• White Epi-light• instant on-screen saturation detection• rapid photographic print production• transilluminator (optional)• White light converter (optional)

Contact your local VWr sales office for advice and a demonstration.

ThermoPrime™ PlusthermoPrime™ plus is a robust recombinant taq polymerase that can amplify fragments in excess of 12 kb, with enhanced thermal properties allowing for improved stability at room temperature. also available in the reddyMix™ format for direct loading on to electrophoresis gels. thermo-Start™ dNa Polymerase is a chemically modified taq Polymerase giving a true hot start enzyme. the chemical modification will give a sensitive, specific reaction with improved yields and reduced non-specific product. thermo Scientific dNtP’s have been developed to high purity standards and provide consistent results across a wide range of molecular biological techniques. available as a mix or separate, and in a variety of concentrations, these reagents will improve the performance of your PCr reaction.

* When purchased at list price.

Buy Taq DNA polymerase & get a

FREE pack of dNTP’s*

PCR Workstation™ designed to protect against contamination in sensitive PCR amplification reactions

promo code7

Easily remove rNase, dNase and dNa contaminates on pipettes, glassware, plasticware etc. with these convenient and effective wipes.

promo code5Stop contamination with RNase

AWAY® or DNA AWAY® Wipes

Buy 2 canisters & get a pack of

wipes free.

Complete monoclonal antibody developmentBy using improved mouse hybridoma technology under following conditions:• 0,2 mg purified protein for the complete project

(includes at least 2 clonings)• time frame 3-4 months • delivery of up to 10 different stable positive, igG producing clones • all project results and clones belong to the customer

*10% on first order through VWr BioScience sales people or direct through BioGenes

10% offfirst order*

promo code9

BioSciencespromo code

8

Description Cat. No. Price

Gel documentation system GenoSmart includes digital video system with integral lCd monitor

730-3000P 5883,00€

Transilluminators

Size (cm) Wavelength (nm) Cat. No. Price

20 x 20 254 730-3017P 1092,00€20 x 30 254 730-3018P 1320,00€25 x 30 254 730-3019P 1500,00€20 x 20 254/ 365 730-3020P 1628,00€20 x 30 254/ 365 730-3021P 1893,00€25 x 30 254/ 365 730-3022P 2159,00€20 x 20 254/312 (302) 730-3023P 1628,00€20 x 30 254/312 (302) 730-3024P 1893,00€25 x 30 254/312 (302) 730-3025P 2159,00€

Cat. No. Dimensions (cm) UV type Price

732-1083P 61 x 61 x 76 single 2157,40€

732-1084P 61 x 61 x 76 dual 2223,33€732-1085P 61 x 61 x 91 single 2386,25€

Cat. No. Dimensions (cm) UV type Price

732-1086P 61 x 61 x 91 dual 2527,42€732-1087P 61 x 61 x 122 single 3039,65€732-1088P 61 x 61 x 122 dual 3217,42€

Introductory prices on the GenoSmart

model

PCR Workstation™ features:• UV irradiation of working area prior to use blocks replication of contaminating

dNa sequences by causing adjacent pyrimidines to undergo dimerization• Protects against cross or airborne contamination by limiting exposure of

experimental set-up to open lab environment• three standard sizes available. dimensions are 61 cm (h) x 61 cm (d) with widths

of 76 cm, 91 cm and 122 cm• High-powered dual UV germicidal lamps with 12 hour countdown timer with

time-hold position• tempered safety glass fascia is hinged for easy access of interior workspace for

cleaning and placement of large instruments• duplex electrical outlet and two fluorescent lamps are mounted in a stainless

steel ceiling• acid-resistant black Formica work surface• Single or double access door(s) close to prevent contamination, then conveniently slide into storage compartment in base

during experimental procedures• Stainless steel ceiling increases UV dose on workstation surface • accessory table and accessory locking casters transform the PCr Workstation™ into a moveable work area that doesn’t

infringe on valuable bench-top space. available in two heights, 71 cm and 91 cm

Free replacement germicidal and

fluorescent bulbs with every purchase

Description Qty/Pk Cat. No. Price

rNase WaY® Canister 25 Wipes 115-1250P 32,60€dNa aWaY® Canister 25 Wipes 115-1252P 32,60€


No skirt, 300 µl 10 732-0661P 31,60€No skirt, elevated wells, 300 µl 10 732-0662P 31,60€No skirt – qPCr compatible, 300 µl 25 732-1024P 68,90€No skirt, low profile, qPCr compatible, clear, 220 µl 25 732-1021P 59,00€No skirt, low profile, qPCr compatible, black, 220 µl 25 732-1023P 68,90€No skirt, low profile, qPCr compatible, white, 220 µl 25 732-1022P 68,90€Segmented, 280 µl 10 732-0663P 31,60€Elevated skirt, 300 µl 10 732-0665P 37,70€Half skirt, low profile, qPCr compatible, clear, 220 µl 25 732-1015P 123,00€Half skirt, low profile, qPCr compatible, black, 220 µl 25 732-1016P 123,00€Half skirt, low profile, qPCr compatible, white, 220 µl 25 732-1017P 123,00€Half skirt, qPCr compatible, 300 µl 10 732-0803P 37,70€Full skirt , 200 µl 10 732-0664P 31,60€

Description Qty/Pk Cat. No. Price Promo Price

Compression mat, 96 well, for real-time PCr 10 732-1020P 59,80€ 44,85€Sealing mat for 96-well PCr microplates 10 732-0668P 38,00€ 28,50€96-well silicone compression mat 10 732-0671P 38,00€ 28,50€96-well silicone expansion mat 10 732-0672P 38,00€ 28,50€UltraClear film, pressure sensitive 100 732-0657P 253,00€ 189,75€


thermoPrime® Plus dNa Polymerase 250 U 733-0020P 109,00€thermo-Start™ dNa Polymerase 250 U 733-0059P 147,00€dNtP Mix - 100mM 1 ml 733-0069P 166,00€

ISSuE 9 - NOVEMBER 2007Shop!



3

Optimise your immunoassaysMethods like Enzyme-linked immunosorbent assays (EliSa), Enzyme immunoassays (Eia), Western blotting, radioimmunoassays (ria), Protein-arrays, immunohistochemistry or the immuno-polymerase chain reaction (immuno-PCr) rely on immuno detection technology. Even in highly specific reactions, there is still the potential for tests to be disturbed by unwanted cross-reactivities.

Crossdown™ Buffer blocks most interference and results can be improved by using it in tandem with Blocking Solution i.

10% off

ThermoScientific Multiskan EX and Wellwash 4 Mk 2 - A proven solution for ELISA assays!

Get 15% off

promo code15

Nunc OptiCell® unique cell culture format for growing, monitoring and transporting cellsoptiCell® is a sterile, sealed cell environment between two optically clear gas-permeable growth surfaces in a standard microtitre plate-sized frame; self-sealing ports provide easy aseptic access to the interior and contents.

Key benefits• Saves time and money by reducing the steps and equipment needed for cell culture• reduces space and media requirements • Geometry optimises robotic handling• Compatible for use with standard, phase contrast, confocal, and high-resolution time-lapse video microscopes

The same tube you have always trusted with a NEW innovative cap design…

BD Falcon™ 50 ml Conical Tube with Flip-Top Capthis novel design saves time and effort for applications requiring multiple aliquoting, storage, and pouring from the same tube, while maintaining the same superior quality and performance of our standard screw-cap closure.

The Flip-Top Cap advantage:• Easy-to-use – one hand cap manipulation is virtually

effortless with the unique hinge action of the flip-top cap• Easy-to-read blue graduations on the tube allow rapid

volumetric assessment• Splash-Guard – clever design of the cap allows opening and closing of the cap

without risk of splatter• Secure seal – innovative click-bead ensures a tight seal every time• ambidextrous – thumb grips on each side of the cap ensure a sterile opening

technique.

laboratory wall clock22 x 24 x 1 cm

promo code10

Free blocking buffer

Detection of the antigen CaMK II in lysates of heart and brain tissue from C57Bl/6 mice. Left: TBS-Tween with 5 % nonfat dried milk. Right: CrossDown™ Buffer in combination with Blocking Solution I.(Data from Dr. Tronhung Quang, Dr. Rainer Klocke, Prof. Dr. Sigrid Nikol, University of Münster, Dept. Cardiology.)

promo code13

BD Falcon™ 50 ml Polypropylene Conical Tube with Flip-Top Cap: Simply flip the cap open and pipette your sample.

Free clock with every 2 cases purchased*

*offer limited to the first 50 customers – one clock per customer

Purchase either the Multiskan EX microplate reader or the Wellwash 4 Mk 2 microplate washer and get 15% off.

the unique design allows mixing horizontally, vertically or any position in between, with a simple turn of the rotisserie plate. the VWr Collection tube rotator has an ambient operating range of +4 ºC to 65 ºC and can be easily transported from the bench to a cold room, incubator or biological hood. the moulded housing and rotisserie assemblies are easy to clean. the speed is fixed to 20 rpm and the maximum load is 0,8 kg. Supplied with a rotisserie assembly that holds 36 x 1,5/2,0 ml tubes, with other sizes up to 50 ml available.

• Unique adjustable rotisserie assemblies

• Variable horizontal and vertical positioning

• Safe for cold room and incubator use

15% off rotator, 30% off accessories

Great value

The new VWR Collection tube rotator

Cell Biology

NEWRemove the burden of cleaning plastic

tanks in biopharmaceutical manufacturing

Buy now! Get 15% introductory discount

a complete line of NalGENE® tanks and matching tank liners ensure that your biopharmaceutical and diagnostic reagents can be prepared and mixed in a guaranteed clean container. the open bags with flat bottom design make fluid processing easy. Manufactured in co-extruded PE film, free of animal-derived components, these tank liners are non-cytotoxic, USP Class Vi & food-grade, and are offered as gamma-irradiated/non-pyrogenic or non-sterile.

these disposable NalGENE® tank liners allow our biopharmaceutical and diagnostic customers to upgrade the cleanliness of their mixing containers for their most valuable solutions. Eliminating the need for the validation of a tank cleaning process therefore reducing cost. all tank liners are individually bagged and are packed 10 units/case.

NALGENE Tank Liners

promo code16

Laboratory supplies

Plan your experiments in advance and get 45% discount on standard peptide services

Be smart - up to 45% off Without loss of quality!

NEW! Plan your experiments 4 to 7 weeks in advance and benefit from this new service. Simply mention the code VWr035 and if you are one of the first 50 orders you will also receive a free 128 MB USB-Stick.*

For more information refer to BioMarke magazine or email [email protected]. Thermo Scientific Biopolymers - Quality you can trust

* only first 50 ProjectPeptide™ orders mentioning the code VWr035 will receive a 128 USB Stick. USB stick not exchangeable for cash. offer valid from 1/11/2007 - 21/1/2008.

Adapt the service to your needs. Our solution: ProjectPeptides™

promo code11

128 MB USB-Stick for free*


Bd Falcon™ 50 ml polypropylene conical tubes with flip-top cap

440 734-1437P 135,56€

BioSciences

Gamma irradiated 25-40 kGy

Tank Liner (non-sterile) (l)

Units/Case

Cat. No. Price Promo Price

19 10 216-2907P 227,00€ 192,95€28 10 216-2908P 238,00€ 202,30€38 10 216-2909P 243,00€ 206,55€57 10 216-2910P 251,00€ 213,35€113 10 216-2911P 378,00€ 321,30€208 10 216-2912P 408,00€ 346,80€303 10 216-2913P 440,00€ 374,00€378 10 216-2914P 467,00€ 396,95€568 10 216-2915P 578,00€ 491,30€757 10 216-2916P 665,00€ 565,25€

Tank Liner (sterile) (l)

Units/Case


19x 10 216-2917P 238,00€ 202,30€113 10 216-2918P 249,00€ 211,65€208 10 216-2919P 254,00€ 215,90€303 10 216-2924P 262,00€ 222,70€378 10 216-2925P 389,00€ 330,65€568 10 216-2926P 419,00€ 356,15€757 10 216-2927P 451,00€ 383,35€303 10 216-2928P 477,00€ 405,45€378 10 216-2929P 589,00€ 500,65€568 10 216-2930P 676,00€ 574,60€

promo code14

promo code12

Non-sterile

Description Cat. No. Price Promo Price

VWr tube rotator, EU plug 444-0500P 299,00€ 254,15€assembly for 10 x 15/10 ml tubes and 16 x 5/7 ml tubes

444-0504P 35,00€ 24,50€

assembly for 6 x 50 ml tubes 444-0505P 35,00€ 24,50€

Description Qty Cat. No. Price

Crossdown buffer plus 50 ml blocking solution i free!

125 ml 999-9940P 149,00€

Crossdown buffer plus 125 ml blocking solution i free!

500 ml 999-9941P 573,00€

Description Pk Cat. No. Price Promo Price

optiCell® 1100 Starter kit 734-2221P 211,00€ 179,35€20 734-2222P 162,00€ 137,70€100 734-2223P 806,00€ 685,10€500 734-2224P 4030,00€ 3425,50€

optiCell® Max 2100 Starter kit 734-2225P 106,00€ 90,10€20 734-2226P 211,00€ 179,35€100 734-2227P 1060,00€ 901,00€

Description Cat. No. Price Promo Price

Wellwash 4MK2 735-0438P 5400,00€ 4590,00€Multiskan Ex 735-0434P 6270,00€ 5329,50€

4

ISSuE 9 - NOVEMBER 2007Shop!

VWR International Eurolab S.LApartado 08100Mollet del Vallés - Barcelona

Place your order today!Tel.: +34 902 222 897Fax: +34 902 430 657http://es.vwr.come-Mail: [email protected]



these offers cannot be used in conjunction with any other pricing agreements from VWr international. Prices exclude Vat.

order from the Customer Service Centre or your VWr Primary distributor:

MediaKap® Hollow Fiber Media FiltersFor fast sterile filtration of media MediaKap® filters are designed to dramatically reduce the time required for sterilising and clarifying tissue culture media and other aqueous solutions. the filter comes in a full range of sizes with capacities from 2 to 50 litres. Spectrum offers two types of filters: MediaKap® and MediaKap® PlUS. the MediaKap® PlUS filters sterilise the same amount of dMEM in almost the same time but with 10% fetal bovine serum. the filter-fiber has a protein-binding of < 2 µg/cm2. radiation sterilised so no Eto residuals, all components pass U.S.P. XX1 class Vi toxicity test and the filters are non-pyrogenic by lal.

New AcroSepTM Chromatography Columns

the new acroSep™ Chromatography Columns for ion exchange, are ideal for optimisation studies of protein purification schemes using small sample volumes prior to scale-up and offer an efficient and reliable method for sample preparation of proteins for structural, functional and yield analysis.

the ion exchange columns contain Pall’s Hyperd chromatography sorbents which are created using patented “gel-in-a-shell” technology, resulting in a column which offers high dynamic binding capacities at fast flow rates for greater productivity at competitive resolution. the acroSep prepacked columns are made with a luer inlet and outlet for easy connection and versatile use:

• Fully automated - in combination with an automated chromatography instrument such as the ÄKta design* systems

• Semi-automated - in combination with pumps or manually with a syringe

promo code20

*while stocks last

promo code21Enhance your protein microarray applications

with Whatman FAST® SlidesFaSt® Slides are glass slides coated with a proprietary nitrocellulose polymer that maintains reactivity of proteins and ensures excellent, reproducible results. they offer significant advantages over traditional methods including improved sensitivity, linearity and quantitation. Many hundreds or thousands of antibodies or samples can be screened at the same time.

Free Soduku game with every

order

Features• Superior protein binding capacity• Highest sensitivity and dynamic range• Excellent long-term stability of printed proteins• Compatible with all detection methodologies• Compatible with commercially available

arraying robots

* Bar coded

Protein InvestigatorTM Protein investigator is a rapid, accurate and in-depth study of a protein from its sequence. the service includes the prediction of the structural and functional properties of your target, and will efficiently collect the maximum relevant information for your protein.

Money-back guarantee. If Protein Investigator does not bring you any useful information about your protein, you don’t pay!

BioSciences

ProteoExtract® Subcellular Proteome Extraction Kit

proteome fractions that are enriched in cytosolic, membrane/organelle, nuclear, and cytoskeletal proteins. in the case of adherent cells, the procedure is performed directly in the tissue culture dish without the need for cell removal. For suspension grown cells, extraction starts with gentle sedimentation and washing of cells. For tissues, the isolation of viable cells is required before proceeding with the extraction protocol.

the especially mild S-PEK procedure results in non-denatured proteins suitable for many downstream applications.

*one timer per order. offer valid while stocks last.

For full technical details of these, and other, sample preparation kits for protein research, please contact your local VWr sales specialist.

promo code19

Proteomics

the ProteoExtract® Subcellular Proteome Extraction Kit (S-PEK) is designed for reproducible extraction of subcellular

proteomes from mammalian cells. Based on different solubilities of certain subcellular compartments the S-PEK uses proprietary chemistries to yield four sub-

Free lab timer with any kit purchased*

Introducing BRAND´s new multichannel pipettesTransferpette® S-8/-12• Central pipetting button and separate tip ejector• Ergonomic finger rest for a relaxed grip for right- or left-

handed users• Effortless one-handed operation. Setting and locking

volume, pipetting, ejectings – all without shifting hand position

• Easy-to-read 4-digit volume display for highest precision• Complete autoclavability at 121°C (20 min.)• Easy calibration technique providing calibration

adjustments without tools, and a clear indication when the pipettes have been adjusted from factory settings

• Compatible with most common tips CE-iVd-compliant

Introductory offer!*Every transferpette® S -8 or transferpette® S -12 comes with one single-channel transferpette® S free!

NEW

Items supplied: Transferpette® S -8 or Transferpette® S -12, conformity certified, performance certificate, 1 filled Tip-Box N, 1 Tip-Rack refill unit, 1 shelf/rack mount, 1 reagent-reservoir, 1 set of sealing rings made of FKM

promo code18

No. of channels Surface Area (cm2)

Suggested Volume (l)*

Filling Bell

Qty/Pk


MediaKap®

MediaKap-2 35 0,2 - 2 Yes 12 734-0869P 117,00€ 105,30€No 18 734-0868P 150,00€ 135,00€

MediaKap-5 70 2 - 5 Yes 12 734-0871P 236,00€ 212,40€No 18 734-0870P 269,00€ 242,10€

MediaKap-10 100 5 - 10 Yes 12 734-0873P 294,00€ 264,60€No 18 734-0872P 498,00€ 448,20€

MediaKap-25 185 10 - 25 Yes 6 734-0874P 272,00€ 244,80€MediaKap-50 440 25 - 50 Yes 3 734-0875P 195,00€ 175,50€

MediaKap® PLUS

MediaKap-2 PlUS 35 0,2 - 2 Yes 12 734-3570P 149,00€ 134,10€

No 18 734-1324P 246,00€ 221,40€

MediaKap-5 PlUS 70 2 -5 Yes 12 734-0876P 244,00€ 219,60€

No 18 734-3571P 403,00€ 362,70€

MediaKap-10 PlUS 100 5 - 10 Yes 12 734-0877P 374,00€ 336,60€



promo code17

promo code22

Multichannel Transferpette® S No. of channels

Volume (µl) Accuracy ≤ ± % Coefficient of variation ≤ %

Single-channel Transferpette® S (µl) included free!

Cat. No. Price

8 0,5 - 10 1,6 1,0 0.5 - 10 613-3710P 495,00€8 5 - 50 0,8 0,4 10 - 100 613-3711P 495,00€8 10 - 100 0,8 0,3 10 - 100 613-3712P 495,00€8 20 - 200 0,8 0,3 20 - 200 613-3713P 495,00€8 30 - 300 0,6 0,3 20 - 200 613-3714P 495,00€12 0.5 - 10 1,6 1,0 0.5 - 10 613-3715P 570,00€12 5 - 50 0,8 0,4 10 - 100 613-3716P 570,00€12 10 - 100 0.8 0,3 10 - 100 613-3717P 570,00€12 20 - 200 0,8 0,3 20 - 200 613-3718P 570,00€12 30 - 300 0,6 0,3 20 - 200 613-3719P 570,00€

Free USB vacuum cleaner kit with every

AcroSep order*

Description Colour Qty/Pk Cat. No. Price

CM Ceramic Hyperd F Green 5 569-1000P 105,00€S Ceramic Hyperd F Sky Blue 5 569-1001P 105,00€Q Ceramic Hyperd F red 5 569-1002P 105,00€dEaE Ceramic Hyperd F orange 5 569-1003P 105,00€

Description Dim. (mm) Qty/Pk Cat. No. Price

FaSt® Slides – 1 pad* 20 x 51 20 737-0000P 406,00€FaSt® Slides – 2 pad* 20 x 20 10 732-0941P 245,00€FaSt® Slides – 8 pad 6 x 6 10 732-0942P 277,00€FaSt® Slides – 16 pad 6 x 6 10 732-0333P 310,00€

FREE

* the suggested volumes for the MediaKap® PlUS filters are for serum-enriched media

-10% off

ProteoExtract® Subcellular Qty/Pk Cat. No. Price

Proteome extraction kit 20 rxn 539-9901P 391,00€Proteome extraction kit, mini 10 rxn 539-9902P 211,60€

Documents

Shop! - es.vwr-cmd.comes.vwr-cmd.com/.../biomarke/0711/BioMarkeShop9_ES.pdf · performance. all cyclers contain the active Sample Cooling (aSC) function that cools your sample until