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http://it.vwr.com Promotions for Life Scientists Issue 8 - September 2007 ISSUE 8, SEPTEMBER 2007 VALID UNTIL 30TH NOVEMBER 2007 CHECK OUT WWW.VWR.COM FOR FULL SPECIFICATIONS For Nordic countries prices are indicative, local price-agreements apply. Cell Biology Cell Biology · VWR Collection cryopreservation package · Calbiochem antibodies from Merck Biosciences · Nalgene ® MF75 PES Filter with asymmetric Supor ® machV PES membrane · Thermo/ biopolymers custom polyclonal peptide antibody service · NEW BD fluorescent dyes · Flourometer and luminometer special plates offer · Get the new catalogues from Nunc and Nalgene Electrophoresis · Horizontal mini-gel system from CBS Molecular biology · Maxymum recovery filter tips from Axygen · NEW VWR Collection thermal cyclers · PCR Mycoplasma Test Kit from Applichem · Brand products for PCR · Abgene, Thermo-fast ® PCR plates · Evaporation cuvettes from BTX · Special kit offers from 5 Prime · Whatman FAST ® slides for microarray · Omega Bio-tek DNA extraction kits Biosciences · Free coffee mug! · Protein analysis from Alphalyse · Tecan plate readers Sample Prep · Pall AcroPrep 96 well filter plates · Spectra/mesh ® woven filters Editor VWR International Europe bvba Haasrode Researchpark Zone 3 Geldenaaksebaan 464 3001 Leuven Belgium Copywriting VWR International Layout and typesetting Marketing Services VWR Printing Stork, Germany No part of this publication may be reproduced or copied without prior permission by writing of VWR International Europe. Run 80.500 copies Publication date: September 2007 Due to the high sales volume of promoted articles some items may be temporarily out of stock - VWR Terms and Conditions of Sale apply. IN THIS ISSUE EDITORIAL IT The one and only! In the CryoPro DPF 30 you can store your samples in a completely dry and uniform temperature environment. The VWR Collection CryoPro DPF 30 is the only portable, self-filling unit that provides a completely dry storage at -190 °C. No liquid Nitrogen or vapour in the storage compartment Fully portable – on wheels! Low pressure allows storage at your workplace (according to EC directive) No splashes of LN 2 – superior safety The EU directive 2004/23/EC states steps should be taken to avoid cross contamination and temperature gradients that could affect the sample material. CryoPro DPF 30 is in complete compliance with this directive. Description Cat. No. Price Freezer pack 1 for 2 ml vials. Includes: Personal freezer DPF 30 478-0150 Set of racks for 1536 x 2 ml vials 478-0151 478-9990P 8 packs of 250 x 2 ml vials 479-0287 Freezer pack 2 for 1,2 ml vials. Personal freezer DPF 30 478-0150 Set of racks for 1920 x 1,2 ml vials 478-0152 478-9991P 8 packs of 250 x 1,2 ml vials 479-0286 15.477,70 15.477,70 Shop! 20% off, including racks and 2000 vials! promo code 5 NALGENE ® MF75 PES filter with asymmetric Supor ® machV PES membrane The NALGENE MF75 filters with Supor ® machV PES membrane offer a complete range of vacuum filtration units for the cold sterilisation of liquid cell culture media, animal sera, growth factors, buffers and solutions for cell and tissue culture work. The asymmetric Supor ® machV 0,2 μm PES membrane enables very high filtration rates and throughput, whilst ensuring that valuable proteins are not retained by the membrane. Even your most concentrated protein-containing solutions can be filtered to the last drop with diameters of 50, 75 or 90 mm to choose from. The NALGENE ® filter units and receiver bottles are certified and radiation-sterilised to guarantee a shelf life of 5 years. With so many sizes to choose from, there is always a NALGENE ® filter unit that is perfect for your filtration needs. Fast and low-binding 0,2 μm Supor ® machV PES membrane for great filtration performance Non-cytotoxic, non-pyrogenic & sterile filter units and receiver bottles Product and lot identification printed on the upper part for full traceability Ergonomic and stable design of leakproof receiver bottles, preventing pH-shift of stored solutions • NALGENE ® Certified performance: Certificate of Quality in each box Supplied in boxes of 12 Supor ® machV is a registered trademark of Pall Corporation. Buy 4 cases, pay for only 3! Description Cap. (ml) Membrane dia. (mm) Cat. No. Price Conical filter unit 50 50 514-0590P Complete filter unit 115 50 514-0023P 150 50 514-0024P 500 75 514-0025P 1000 90 514-0026P 250 50 514-0027P 500 90 514-0028P Bottle top filter - 33 mm bottle neck 500 75 514-0029P Bottle top, 45 mm thread 500 75 514-0030P Bottle top, 33 mm thread 150 50 514-0031P Bottle top, 45 mm thread 150 50 514-0032P Bottle top, 33 mm thread 1000 90 514-0033P Bottle top, 45 mm thread 1000 90 514-0034P 52,00 34,00 44,00 82,00 104,00 51,00 97,00 48,00 48,00 35,00 35,00 92,00 92,00 20% discount on primary antibodies from Calbiochem* See our full antibody range in the Antibody SourceBook (5th Edition): More than 2000 primary antibodies listed with species reactivity and application data Secondary antibodies Antibody support products * To obtain your 20% discount add P to the end of the Calbiochem catalogue number when ordering. Valid for regular sizes only, trial sizes are excluded from this offer Time flies! Welcome to the Autumn edition of bioMarke Shop. We hope that you have had an excellent summer. Now as the days get shorter and the weather a bit colder and wetter our aim is to brighten your day by bringing you the brightest and best promotions and offers for Life Science products as a companion to our journal the bioMarke magazine. Don’t miss out on the fantastic VWR Collection cryopreservation package, new catalogues and free coffee mugs. Enjoy! Your VWR bioMarke team Custom Polyclonal Peptide Antibody Service promo code 2 Buy 3 antibodies but pay for only 2! PLUS Be one of the first 10 placing an order and receive a 1 GB USB stick FREE of charge!* Test our NEW antibody production service and benefit from the high quality and the special 3 for 2 offer. For more information go to www.thermo.com/antibodies or contact your local VWR specialist. *Terms and Conditions: 3 for 2 offer only valid for orders placed before 30/11/2007 and only if placed by email at [email protected] using the Excel order form available at www.thermo.com/antibodies.You MUST quote the reference VWR031 to participate in this offer and to claim the free USB stick if you are one of the first 10 orders. Prizes not exchangeable for cash. Void when prohibited by law or company / organisation. Ask for our FREE Prediction Service! NEW promo code 3 NEW BD Fluorescent Dyes - live cell tracking in cell-based assays BD Biosciences introduces their NEW BD Calcein AM and BD DiIC 12 (3) Fluorescent Dyes. The non fluorescent, cell permeant Calcein AM is converted in the cell into a fluorescent anion. The lipophilic tracer DiIC 12 (3) labels neuronal projections as well as lipid bilayers in other cell types. BD Fluorescent Dyes can be used with BD BioCoat Angiogenesis Systems or with other cell-based assays to pre- or post-label viable cells and quantify cell migration, invasion and endothelial cell tubulogenesis. Description Qty/Pk Cat. No. Price Promo price BD Calcein-AM 10 x 50 μg 734-1434P BD Calcein-AM 1 mg 734-1435P BD DiIC 12 (3) 100 mg 734-1436P 288,00 244,80 322,00 273,70 356,00 302,60 15% off promo code 1 Order your copy from your local VWR office. promo code 4 Prices reduced!

Shop! - es.vwr-cmd.comes.vwr-cmd.com/.../life_science/biomarke/0709/bioMarkeShop8_IT.pdf · from the high quality and the special 3 for 2 offer. For more information go to www

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http://it.vwr.com

Promotions for Life Scientists Issue 8 - September 2007

ISSUE 8, SEPTEMBER 2007 • VALID UNTIL 30TH NOVEMBER 2007 • CHECK OUT WWW.VWR.COM FOR FULL SPECIFICATIONSFor Nordic countries prices are indicative, local price-agreements apply.

Cell Biology

Cell Biology· VWR Collection cryopreservation package · Calbiochem antibodies from Merck Biosciences· Nalgene® MF75 PES Filter with asymmetric

Supor® machV PES membrane· Thermo/ biopolymers custom polyclonal

peptide antibody service· NEW BD™ fluorescent dyes · Flourometer and luminometer special

plates offer· Get the new catalogues from Nunc and

Nalgene

Electrophoresis· Horizontal mini-gel system from CBS

Molecular biology· Maxymum recovery filter tips from Axygen· NEW VWR Collection thermal cyclers· PCR Mycoplasma Test Kit from Applichem · Brand products for PCR · Abgene, Thermo-fast® PCR plates· Evaporation cuvettes from BTX· Special kit offers from 5 Prime· Whatman FAST® slides for microarray · Omega Bio-tek DNA extraction kits

Biosciences· Free coffee mug!· Protein analysis from Alphalyse· Tecan plate readers

Sample Prep· Pall AcroPrep™ 96 well filter plates· Spectra/mesh® woven filters

EditorVWR International Europe bvbaHaasrode Researchpark Zone 3Geldenaaksebaan 4643001 LeuvenBelgium

CopywritingVWR International

Layout and typesettingMarketing Services VWR

PrintingStork, GermanyNo part of this publication may be reproduced or copied without prior permission by writing of VWR International Europe.

Run80.500 copiesPublication date: September 2007Due to the high sales volume of promoted articles someitems may be temporarily out of stock - VWR Terms andConditions of Sale apply.

IN THIS ISSUE

EDITORIAL

IT

The one and only!In the CryoPro DPF 30 you can store your samples in a completely dry anduniform temperature environment.

The VWR Collection CryoPro DPF 30 is the only portable, self-filling unit that provides a completely dry storage at -190 °C.

• No liquid Nitrogen or vapour in the storage compartment • Fully portable – on wheels!• Low pressure allows storage at your workplace (according to EC directive) • No splashes of LN2 – superior safety

The EU directive 2004/23/EC states steps should be taken to avoid cross contamination and temperature gradients thatcould affect the sample material. CryoPro DPF 30 is in complete compliance with this directive.

Description Cat. No. Price

Freezer pack 1 for 2 ml vials. Includes: Personal freezer DPF 30 478-0150Set of racks for 1536 x 2 ml vials 478-0151 478-9990P8 packs of 250 x 2 ml vials 479-0287Freezer pack 2 for 1,2 ml vials. Personal freezer DPF 30 478-0150Set of racks for 1920 x 1,2 ml vials 478-0152 478-9991P8 packs of 250 x 1,2 ml vials 479-0286

15.477,70 €

15.477,70 €

Shop!20% off, including

racks and 2000 vials!

promo code

5NALGENE® MF75 PES filter with asymmetricSupor® machV PES membrane

The NALGENE MF75™ filters with Supor® machV PES membrane offer a complete range of vacuum filtration units for the cold sterilisation of liquidcell culture media, animal sera, growth factors, buffers and solutions for cell and tissue culture work. The asymmetric Supor® machV 0,2 μm PESmembrane enables very high filtration rates and throughput, whilst ensuring that valuable proteins are not retained by the membrane. Even yourmost concentrated protein-containing solutions can be filtered to the last drop with diameters of 50, 75 or 90 mm to choose from.

The NALGENE® filter units and receiver bottles are certified and radiation-sterilised to guarantee a shelf life of 5 years.With so many sizes to choose from,there is always a NALGENE® filter unitthat is perfect for your filtration needs.

• Fast and low-binding 0,2 μm Supor®

machV PES membrane for great filtration performance

• Non-cytotoxic, non-pyrogenic & sterile filter units and receiver bottles

• Product and lot identification printed on the upper part for full traceability• Ergonomic and stable design of leakproof receiver bottles, preventing

pH-shift of stored solutions• NALGENE® Certified performance: Certificate of Quality in each box• Supplied in boxes of 12

Supor® machV is a registered trademark of Pall Corporation.

Buy 4 cases,pay for only 3!

Description Cap. (ml) Membrane dia. (mm) Cat. No. PriceConical filter unit 50 50 514-0590PComplete filter unit 115 50 514-0023P

150 50 514-0024P500 75 514-0025P1000 90 514-0026P250 50 514-0027P500 90 514-0028P

Bottle top filter - 33 mm bottle neck 500 75 514-0029PBottle top, 45 mm thread 500 75 514-0030PBottle top, 33 mm thread 150 50 514-0031PBottle top, 45 mm thread 150 50 514-0032PBottle top, 33 mm thread 1000 90 514-0033PBottle top, 45 mm thread 1000 90 514-0034P

52,00 €34,00 €44,00 €82,00 €

104,00 €51,00 €97,00 €

48,00 €48,00 €35,00 €35,00 €92,00 €92,00 €

20% discount on primary antibodies from Calbiochem*See our full antibody range in the Antibody SourceBook (5th Edition):• More than 2000 primary antibodies listed with species reactivity and application data • Secondary antibodies• Antibody support products

* To obtain your 20% discount add P to the end of the Calbiochem catalogue number when ordering.Valid for regular sizes only, trial sizes are excluded from this offer

Time flies! Welcome to theAutumn edition of bioMarkeShop. We hope that you have hadan excellent summer. Now as thedays get shorter and the weathera bit colder and wetter our aim isto brighten your day by bringingyou the brightest and bestpromotions and offers for LifeScience products as a companionto our journal the bioMarkemagazine. Don’t miss out on thefantastic VWR Collectioncryopreservation package, newcatalogues and free coffee mugs.Enjoy!

Your VWR bioMarke team

Custom Polyclonal PeptideAntibody Service

promo code

2

Buy 3 antibodies but pay for only 2! PLUS Be one of the first 10 placing an order and receive a 1 GB USB stick FREE of charge!*

Test our NEW antibody production service and benefitfrom the high quality and the special 3 for 2 offer.For more information go to www.thermo.com/antibodiesor contact your local VWR specialist.

*Terms and Conditions:3 for 2 offer only valid for orders placed before 30/11/2007 and only if placed by email at [email protected] using theExcel order form available at www.thermo.com/antibodies. You MUST quote the reference VWR031 to participate in this offer and toclaim the free USB stick if you are one of the first 10 orders. Prizes not exchangeable for cash. Void when prohibited by law orcompany / organisation.

Ask for our FREE Prediction Service!

NEW

promo code

3NEW BD™ Fluorescent Dyes - live cell tracking in cell-based assaysBD Biosciences introduces their NEW BD™ Calcein AM and BD™

DiIC12(3) Fluorescent Dyes. The non fluorescent, cell permeantCalcein AM is converted in the cell into a fluorescent anion. Thelipophilic tracer DiIC12(3) labels neuronal projections as well aslipid bilayers in other cell types.

BD™ Fluorescent Dyes can be used with BD BioCoat™

Angiogenesis Systems or with other cell-based assays to pre- or post-label viable cells and quantify cell migration, invasion and endothelial cell tubulogenesis.

Description Qty/Pk Cat. No. Price Promo price

BD™ Calcein-AM 10 x 50 μg 734-1434PBD™ Calcein-AM 1 mg 734-1435PBD™ DiIC12(3) 100 mg 734-1436P

288,00 € 244,80 €322,00 € 273,70 €356,00 € 302,60 €

15% off

promo code

1

Order your copyfrom your local

VWR office.

promo code

4

Prices reduced!

ISSUE 8 - SEPTEMBER 2007

Offers valid from 1st September to 30th November 2007 Place your order today! Visit our website http://it.vwr.comPromotions in the VWRbioMarke SHOP may not be used in combination, VWR reserves the right to refuse invalid orders. For Nordic countries prices are indicative, local price-agreements apply.2

Shop!Electrophoresis

promo code

10Free PCR plates when you purchase Axygen Maxymum Recovery filter tips Buy two packs of the same Axygen Maxymum Recovery filter tipsand receive a pack of 96 well unskirted PCR plates absolutely FREE

Why use Maxymum Recovery filter tips?• Allows for complete sample retrieval• Saves costly reagent by reducing sample loss• Greater tip to tip precision• Optimises the accuracy of the pipette• Avoid denaturation of nucleic acids and protein samples• Designed to fit a wide variety of single and multi-channel pipettes

Free PCR plates!

Cell Biology

The Thermo Scientific Fluoroskan Ascent microplate fluorometer and LuminoskanAscent microplate luminometer together with white and black Thermo ScientificMicrotiter plates are powerful combinations to increase sensitivity of ATP andPicogreen assays. To learn more, go to www.thermo.com/readingroom and downloadyour own copy of the two application notes describing these assays.

Special offer:

Purchase the Fluoroskan Ascent microplatefluorometer or the Luminoskan Ascent microplateluminometer and receive a case of plates free!Choose between a pack of 50 white or blackCliniplates, 96 well, universal binding.

Go to www.vwr.com/biomarke and supply us withthe serial number of the instrument you purchasedand we will send you your free case of microplates.

Offer not available in Italy

Increase the sensitivity ofyour fluorometric and luminometric assays!

promo code

6 Free accessories to cast a second gel whilstthe first is still running!

promo code

8

Buy a horizontal mini-gel kit and get a FREE external gel caster and extra UVT gel tray!

Ideal for separation and identification of DNA and RNA fragments, PCRproducts and synthetic oligonucleotides. Features tapered baffles forsubmarine gel formation and safety cover with attached power leads.This special offer also includes a 1,0 mm thick dual-sided comb allowinggel formation of either 10 or 14 wells.

Size (μl) Type Pack size Cat. No. Price

10 Universal fit 10 x 96 732-0649EU10 Extended length 10 x 96 732-0650EU10 Eppendorf style 10 x 96 732-0651EU20 Eppendorf style 10 x 96 732-0936EU20 Universal (except Eppendorf) 10 x 96 732-0652EU100 Universal (except Eppendorf) 10 x 96 732-0862EU200 Universal fit 10 x 96 732-0655EU1000 Universal fit 10 x 100 732-0863EU1100 Universal fit 12 x 96 732-0935EU

55,00 €55,00 €55,00 €55,00 €55,00 €55,00 €55,00 €55,00 €65,00 €

Request the new catalogues now on www.vwr.com

PCR Mycoplasma test kitpromo code

7

Description Cat. No. Price

PCR Mycoplasma test kit APPCA3744.0020P 300,00 €

Find your way around the research LAByrinth

NUNC™ and NALGENE ® provide the broadest range of t rusted lab solut ions

Description Cat. No. Price

Includes a dual-sided comb (1,0 mm x 10/14 well), 2 UVT gel trays, 730-1814Pand external gel caster. 7,5 cm(w) x 10 cm(l)

350,00 €

The PCR Mycoplasma test kit is designed to detect the presence ofmycoplasma contaminating biological materials, in for example cultured cells.

Mycoplasma detection by the direct culture procedure istime-consuming and some mycoplasma species aredifficult to cultivate. PCR testing gives results within a fewhours, since the presence ofcontaminant mycoplasma can beeasily detected simply by verifyingthe bands of amplified DNAfragments in electrophoresis. Thereis no need to prepare probeslabelled with radioisotopes, or tocalculate enzyme, dNTP’s or bufferconcentrations. Instead, a ready-to-use, optimised PCR mix issupplied.

The primer set allows detection ofvarious mycoplasma species (M. fermentans, M. hyorhinis, M.arginini, M. orale, M. salivarium,M. hominis, M. pulmonis, M.arthritidis, M. bovis, M. pneumoniae, M. pirum and M. capricolum), as well asAcholeplasma and Spiroplasma species, with high sensitivity and specificity.

PCR-based method

• Simple, one-step reaction; less than 10 minutes to prepare the sample - The mixture contains everything (incl. Taq Pol.)

• Can be used routinely in every lab• Minimal sample handling reducing the risk of contamination• Sensitive (100 – 1000 CFU/ml)• M. hyorhinis can be detected

Buy a PCR Mycoplasma test kit and get the incubator-clean 500 ml solutionto prevent contamination in incubators free (A5230.0500)

FREE incubator-clean 500 ml solution

The thermal cycler collection from VWR is the new solution for the modern molecular biology laboratory, including all the latest features and functionality that will take yourresearch to a new level.

For high throughput, the Duo and QuattroCycler have been developed with two or four independent thermal bays and fully automatedheated lids. Reliable and reproducible thermal performance characteristics should be viewed as the essential elements of a good thermalcycler - the Duo and the QuattroCyclers will deliver results with the highest quality data every time.

Buy a Duo cycler and a 96 wellgradient block, and get a second blockfree of charge.

Or buy the Quattro cycler and two 96well gradient blocks and get anothertwo blocks free.

Please contact your local VWR salesorganisation for a demonstration

The next generation in thermal cyclingtechnology is now available

promo code

9

Introductory offer!

Description Cat. No. Price

DuoCycler thermal cycler chassis w/o block + 96 well gradient block 732-9990PQuattroCycler thermal cycler chassis w/o block+ two 96 well gradient blocks 732-9991P

9.800,00 €

18.600,00 €

Two year warranty

Free case of plates!

Molecular biology

NEW

New

Prices reduced!

Offers valid from 1st September to 30th November 2007 Place your order today! Visit our website http://it.vwr.comPromotions in the VWRbioMarke SHOP may not be used in combination, VWR reserves the right to refuse invalid orders. For Nordic countries prices are indicative, local price-agreements apply. 3

ISSUE 8 - SEPTEMBER 2007Shop!Molecular Biology

Special features of all PCR disposables from BRAND:• Suitable for use in common thermal cyclers• Polypropylene (PP), extra-thin uniform wall thickness to provide the optimal thermal

transfer and short cycle times• Manufactured under the most modern cleanroom conditions• DNase-, DNA- and RNase-free• Autoclavable at 121 °C (20 min.)

Strips of 8 PCR tubes with attached individual caps – suitable for quantitative Real-Time PCR

PP, clear, 8 connected 0,2 ml or 0,15 ml vessels withattached, flat individual caps provide an ideal protectionagainst contamination. Ideal for quantitative Real-TimePCR due to the highly transparent cap.

Save with BRAND productsfor PCR!

Capacity (ml) Description Pack Cat. No. Price Promo Price

0,2 Standard 120 strips (960 vessels) 211-2613P0,15 Low Profile 120 strips (960 vessels) 211-2614P

122,00 € 85,40 €122,00 € 85,40 €

promo code

11

PCR mini cooler with transparent lid and visual indicator - changes colour whentemperature rises.

The PCR mini cooler is made of PP and keeps samplesat 4 °C for approximately 3 hours. The insulating gelchanges colour from violet to pink at 7 °C. Suitable for0,2 ml single vessels, 8-strips and 12-strips as well as96-well PCR plates.

Pack Cat. No. Price Promo Price

2 211-3269P 70.20 € 56,16 €

Save more than 30%

Save more than 20%

ABgene® Thermo-fast® PCR platesWith over 10 years experience in manufacturing PCR plates, Thermo Scientific ABgene® branded plates are worldrenowned for their premium quality and consistent performance.

• Virgin polypropylene ensures uniformity and quality of every plate • Every PCR plate checked for integrity and consistency• Thin wall design for optimum heat transfer ensuring maximum PCR performance• Certified free from DNase, RNase and human genomic DNA• Compatible with all PCR instruments• Supplied in packs of 25 plates

ABgene® products are not available in Italy, Belgium and the Netherlands

promo code

13

Description Skirt Cat. No. Price Promo Price

Thermo-Fast® 96 well PCR plate non 732-4828Psemi 732-0892Psemi 732-4944Pfully 732-4888P

Thermo-Fast® 96 well PCR plate semi 732-0882Pblack grid referencing fully 732-0881P

30% off!

Got cuvettes?BTX electroporation cuvettes plusHigh quality cuvettes for transfection/transformation of all cell types including bacteria, yeast, plantand mammalian cells. Compatible with most electroporation systems.

• Transfer pipettes included with each cuvette• Round caps: easy to handle and fits perfectly• Colour coded: easy identification• Available in 1, 2, and 4 mm gap sizes• Gamma radiated for guaranteed sterility• Medical-grade polycarbonate• Smooth-polish aluminum plates• Supplied in packs of 50

promo code

14

Gap (mm) Cat. No. Price

1 732-0020P2 732-0021P4 732-0023P

158,00 €158,00 €158,00 €

Buy 3 bags of BTXcuvettes and pay

for only 2

It's time for 5 Prime - your primepartner for perfect researchAs the one source for your everyday laboratory research, 5 PRIME offers former Eppendorf or Gentra reagents for:• Nucleic acid purification: plasmid DNA, genomic DNA,

& RNA• PCR: End point & Real-Time PCR/RT-PCR• Molecular biology reagents: buffers & enzymes

5 PRIME’s wide range of PCR & RT-PCR enzymes ensures that you will find the optimalenzyme for your application:

* Offer limited to one per customer

promo code

15

Buy one kit,get another one

free!*

Application Product Size Cat. No. Price

Standard PCR & RT-PCR Taq DNA polymerase 250 units 733-0107PHot-Start PCR – highest PCR specificity HotMaster Taq DNA polymerase 250 units 733-0118PDifficult & contaminated samples MasterTaq kit 250 units 733-0114PProofreading Exact polymerase 100 units 733-0993PLong range PCR PCR extender system 200 units 733-0190PReverse transcription Masterscript kit 200 rxns 733-0997POne-step & two-step RT-PCR Masterscript RT-PCR system 100 rxns 733-0998P

81,10 €124,00 €102,00 €97,40 €

229,00 €930,00 €464,00 €

Enhance your protein microarray applications with Whatman FAST® slidesFAST® slides are glass slides coated with a proprietary nitrocellulose polymer that maintains reactivity of proteins and ensures excellent, reproducible results. They offer significant advantages over traditional methods including improved sensitivity, linearity and quantitation. Many hundreds or thousands of antibodies or samples can be screened at the same time.

• Superior protein binding capacity• Highest sensitivity and dynamic range• Excellent long-term stability of printed proteins• Compatible with all detection methodologies• Compatible with commercially available arraying robots

* Bar coded

promo code

16

Buy a pack ofWhatman FAST®

slides and get a freeSUDOKU game!

Omega Bio-Tek offers DNA extraction kits for any source. Now you have the chance totry out Omega Bio-Tek's plant, soil or stool kits completely free! We know these kitsperform excellently - but we want to convince you to try a free sample and put these kits through your tests.

E.Z.N.A SP plant mini kit: homogeniser column included.Free sample kit - Order OMEGD5511-00P

E.Z.N.A. soil DNA kit: contains a new inhibitor removal agent!Free sample kit - Order OMEGD5625-00P

E.Z.N.A. stool DNA kit: contains a new inhibitor removal agent!Free sample kit - Order OMEGD4015-00P

*limited to one sample kit per customer

Try before you buy!*promo code

12

Pack Cat. No. Price

50 OMEGD5511-01P 200 OMEGD5511-02P

97,00 €348,00 €

Pack Cat. No. Price

50 OMEGD5625-01P 200 OMEGD5625-02P

127,00 €470,00 €

Pack Cat. No. Price

50 OMEGD4015-01P200 OMEGD4015-02P

132,00 €483,00 €

The new standard, with real hot air sterilisation

The Binder C150 sets the “New standards for cell cultivation”. Readthe article in issue 18 of the VWRbioMarke magazine for more detailson this fabulous product.

Description Dimensions (mm) Pack Cat. No. Price

FAST® slides – 1 pad* 20 x 51 20 737-0000PFAST® slides – 2 pad* 20 x 20 10 732-0941PFAST® slides – 8 pad 6 x 6 10 732-0942PFAST® slides – 16 pad 6 x 6 10 732-0333P

434,00 €267,00 €303,00 €339,00 €

ISSUE 8 - SEPTEMBER 2007

Offers valid from 1st September to 30th November 2007 Place your order today! Visit our website http://it.vwr.comPromotions in the VWRbioMarke SHOP may not be used in combination, VWR reserves the right to refuse invalid orders. For Nordic countries prices are indicative, local price-agreements apply.4

Shop!Biosciences

Sample Preparation

For high throughput sample preparation and detection procedures

• Proprietary sealing technology individually seals each well eliminating crosstalk• Available with a variety of membranes and configurations, plate colours, well

volumes and outlet tip lengths• Robotic-friendly design has single-piece construction• Plates are constructed from chemically-resistant,

biologically-inert polypropylene• Serialised barcode label allows for use in automated tracking systems

Ideal for the following applications:• Concentrate, purify and desalt proteins and peptides• Bead-/resin-based applications• Gross fractionation and lysate clarification• General filtration

Pall AcroPrep™ 96 well filter plates

promo code

20

Membrane Pack Cat. No. Price

AcroPrep 96 well filter plates, 350 μl well, naturalOmega 3K 10 516-0169POmega 10K 10 516-6884POmega 30K 10 516-6885POmega 100K 10 516-6886P0,2 μm GHP 10 516-6892P0,45 μm GHP 10 514-0459P 1,0 μm glass fibre 10 516-6882P 1,2 μm Supor® 10 736-0196P3,0 μm GF/0,2 μm Bio-Inert® 10 516-6893PAcroPrep 96 well filter plates, 1 ml well1,0 μm glass fibre 5 516-6894P 0,2 μm GHP, natural 5 516-6895P3,0 μm GF/0,2 μm Bio-Inert® 5 516-6896P0,45 μm GHP, natural 5 516-6897P

289,00 €289,00 €289,00 €289,00 €185,00 €185,00 €127,00 €171,00 €225,00 €

104,00 €116,00 €150,00 €116,00 €

Spectrum® offers four types of synthetic fibres consisting of extruded solid, nonporous, smooth monofilaments that retard trapping impurities and facilitate cleaning. The fibres are woveninto precise meshes with a high proportion of open area to provide accurate and efficient filtering, screening, sifting, classifying, drying, straining, grading and dewatering.You can choose the polymer type based on your application conditions such as pressure, temperature, abrasion, polarity and chemical compatibility.

Product specifications and properties - Spectra/Mesh® macro filtration

One of the most useful Spectra/Mesh® filters in Life Science research is Spectra/Mesh® polyester. The filter material is mildly hydrophobic and has a low binding capacity to proteins. It has special applications for cellular separation, tissue culture, cloning transfer, cellular and bacterial analysis, protein and virus purification, RNA & DNA hybridisation and electron microscopy.

Spectra/Mesh® woven filterspromo code

21

Nylon Polyester Polypropylene Fluorocarbon

Mesh opening (μm) 5 - 1,000 5 - 300 105 - 1,000 70 - 1,000

Water affinity hydrophilic mildly hydrophobic hydrophobic very hydrophobic

Strength/durability high high high (wet) medium

Adsorption high low low (inert) very low (inert)

Best resistance corrosives alkalis/organics acids/alkalis/organics nearly all

pH resistance pH 3 – 10 pH 3 – 13 pH 2 – 14 pH 1 – 14

Thermal stability up to 180 °C up to 140 °C up to 130 °C up to 250 °C

Sterilisation irradiation autoclavable autoclavable autoclavable

15% off all polyester fibres

The VWR Bioscience programme offers a complete portfolio of custom services forcustomers’ scientific discovery and drug development needs.

Please refer to the special insert in the VWRbioMarkemagazine or refer to our VWR Biosciences homepage on vwr.com for a detailed overview of our capabilities.

All enquiries should be referred to your local contact:

Free VWR BioSciences mug tofirst 100 enquiries!

promo code

17

Limited availability!

promo code

18Protein analysis from Alphalyse

Get easy access to protein analysis – choose from seven

services

• Protein identification – from 1D/2D gels or protein in-solution • Characterisation of protein phosphorylation • Characterisation of protein glycosylation • N-terminal sequencing• Amino acid analysis • Protein molecular weight determination• 2D-Gel protein profiling

The Pick ‘n Post Protein Analysis services offered by Alphalyse give fast and easy access to high qualityprotein analysis. You receive a full analysis report by email within 8-10 days (depending on the specificservice).

Order your Pick ‘n Post Protein Analysis kit for free at www.pick-n-post.com and get 100€ off your first order.

Get 100€ off yourfirst order

promo code

19Relax, your reader is a TecanOur 7 different detection modes guarantee top measurement speeds and extremely reliable results,speeding up a wide range of biotech, pharmaceutical, cosmetics and research applications.

See the difference with our highly sensitive, accurateand versatile Infinite™ F500 and Safire2™ microplatereaders.

For more information please contact your localVWR sales office or your local VWR Biosciencesrepresentative and you might get a free mugtoo!

Offer not available in Italy

New range fromVWR Biosciences

Countries Contact

UK, IE [email protected], BE, NL [email protected], NO, FI, SE [email protected], AU, CH [email protected] [email protected], ES [email protected]

Mesh Disc diameter Sheetpore Open Thickness 25 mm 47 mm 55 mm 90 mm 150 mm 30 x 30 cmsize (μm) area % (μm)

10/pkg 5/pkg 3/pkg

5 2 65 734-3510P 734-3513P 734-3524P 734-3532P 734-3563P 734-3518P 10 2 55 734-3511P 734-3514P 734-3525P 734-3533P 734-3520P 734-3542P 15 8 55 734-3512P 734-3515P 734-3526P 734-3534P 734-3564P 734-3543P 21 15 70 734-3549P 734-3556P 734-3527P 734-3535P 734-3521P 734-3544P 37 25 65 734-3550P 734-3557P 734-3528P 734-3536P 734-3565P 734-3519P43 29 70 734-3551P 734-3558P 734-3516P 734-3537P 734-3522P 734-3545P 60 29 87 734-3552P 734-3559P 734-3529P 734-3538P 734-3566P 734-3546P 74 36 90 734-3553P 734-3560P 734-3530P 734-3539P 734-3567P 734-3547P 80 39 90 734-3554P 734-3561P 734-3531P 734-3540P 734-3523P 734-3548P 300 44 258 734-3555P 734-3562P 734-3517P 734-3541P 734-3568P 734-3569P

Price 88,40 € 102,70 € 113,10 € 137,80 € 163,80 € 284,70 €Promo Price 75,14 € 87,30 € 96,14 € 117,13 € 139,23 € 242,00 €

Order from the Customer ServiceCentre or your VWR PrimaryDistributor:These offers cannot be used in conjunction with anyother pricing agreements from VWR International. Pricesexclude VAT.

VWR International s.r.lVia Stephenson 94I - 20157 Milano (MI)

Place your order today!Tel: 02 332 03 11Fax: 800 152 999http://it.vwr.come-Mail: [email protected]: [email protected] per ordinare

Offer not available in Italy

Prices reduced!