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Supplemental Figure 2 A Control RIPC 0.0 0.5 1.0 1.5 M M P-2 (a.u.)/Actin (a.u.) Control RIPC 0.0 0.5 1.0 1.5 M M P-9 (a.u.)/Actin (a.u.) C ontrol R IPC 0.0 0.5 1.0 1.5 M M P-9 (a.u.)/Actin (a.u.) Control RIPC 0.0 0.5 1.0 1.5 M M P-2 (a.u.)/Actin (a.u.) B C D Proteinexpression of MMP-2/9. Westernblotting experiments were performed using cardiac tissue derived before CPB (A, C) and after CPB (B, D). Antibodies against MMP-2 (A, B) and MMP-9 (C, D) were employed and the intensity of the respective signal was related to the intensity of actin. One representative westernblot containing samples from 6 control and 6 RIPC patients is shown in E (before CPB) and F (after E F MMP-9 MMP-2 Actin MMP-9 MMP-2 Actin Control RIPC Control RIPC Before CPB After CPB N=18 N=17 N=18 N=17 N=18 N=17 N=18 N=17

Supplemental Figure 2

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After CPB. Before CPB. N=17. N=18. N=17. N=18. B. A. N=17. N=18. N=17. N=18. D. C. MMP-9. MMP-9. MMP-2. MMP-2. Actin. Actin. RIPC. RIPC. Control. Control. F. E. Supplemental Figure 2. - PowerPoint PPT Presentation

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Page 1: Supplemental Figure 2

Supplemental Figure 2

A

Control RIPC0.0

0.5

1.0

1.5

MM

P-2

(a.

u.)

/ Act

in (

a.u.

)

Control RIPC0.0

0.5

1.0

1.5

MM

P-9

(a.

u.)

/ Act

in (

a.u.

)

Control RIPC0.0

0.5

1.0

1.5

MM

P-9

(a.

u.)

/ Act

in (

a.u.

)

Control RIPC0.0

0.5

1.0

1.5

MM

P-2

(a.

u.)

/ Act

in (

a.u.

)

B

C D

Proteinexpression of MMP-2/9. Westernblotting experiments were performed using cardiac tissue derived before CPB (A, C) and after CPB (B, D). Antibodies against MMP-2 (A, B) and MMP-9 (C, D) were employed and the intensity of the respective signal was related to the intensity of actin. One representative westernblot containing samples from 6 control and 6 RIPC patients is shown in E (before CPB) and F (after CPB). Bars denote SEM.

E F

MMP-9

MMP-2

Actin

MMP-9

MMP-2

Actin

Control RIPC Control RIPC

Before CPB After CPB

N=18N=17

N=18N=17 N=18N=17

N=18N=17

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