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ABSTRACTS. found in the lungs and liver. Early peritonitis with some ascites was also present. The author was unable to of)tain cultures from this animal. The authors conclude that though the organism isolated somewhat resembles the bacillus mallei it is not identical with it. (Edward and Ireland, Abstract from Portions of a Report for the Advisory Committee for the Tropical Diseases Research Fund, November 1912, pp. 2-7.) THE TRANSMISSION OF TO DOG THROUGH SERRATICEPS. LEISHMANIA FROM DOG THE AGENCY OF PULEX IN the first experiment a dog which had been under observation for a period of two months, during which time it had appeared perfectly healthy, was subjected on eighty-two occasions to the bites of fleas which had previously been fed upon infected dogs. Rather more than a month later evidence of infection appeared, and the animal lost condition. Examination of marrow from the femur, however, proved negative. Subsequently the animal's condition improved, and it was then killed. , At the post-mortem parasites in an apparently degenerated condition were found in small numbers in preparations from the spleen and bone-marrow. Cultures on the Novy-M'Neal-Nicolle medium yielded numerous lepto- monads by the eighth day. This strain had up to the time of writing been passed through thirteen subcultures. Two other animals, one of which was bitten daily for fifteen days by a single flea fed upon an infected dog every two days during the preceding fortnight, and the other which was bitten daily for four days by a flea which had had a single meal on an infected dog two days previously, failed to become infected. In the intestinal contents of fleas that had been fed upon infected dogs, and in two out of nine fleas fed upon supposedly healthy dogs, numerous resting forms of the parasite were observed. These possessed a fairly large nucleus and blepharoplast. In the f<ecal matter and in the posterior portion of the intestine slender bi-nucleated flagellates were discovered measuring 10 to 12 microns in length. Transitional forms were also observed. (Sergent (Ed.), Sergent (Et.),Lheritier, and Lemaire, Bull. Soc. Path. Exot., Vol. V., No.8, October 19 12 , pp. 595-597·) HUMAN TUBERCLE BACILLI IN THE MILK OF A VACCINATED COW. AFTER referring at some length to the investigations of Weber and Titze, and to some of the results obtained by the Royal Commission, the author states that recently the opportunity offered to test the milk of two heifers that were vaccinated with human tubercle bacilli as calves. In the milk of one of these tubercle bacilli of the human type were found. The animal was vaccinated when four days old, the inoculation being performed intravenously, and presumably with a minimal dose. She calved

The Transmission of Leishmania from Dog to Dog through the Agency of Pulex Serraticeps

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Page 1: The Transmission of Leishmania from Dog to Dog through the Agency of Pulex Serraticeps

ABSTRACTS.

found in the lungs and liver. Early peritonitis with some ascites was also present. The author was unable to of)tain cultures from this animal.

The authors conclude that though the organism isolated somewhat resembles the bacillus mallei it is not identical with it. (Edward and Ireland, Abstract from Portions of a Report for the Advisory Committee for the Tropical Diseases Research Fund, November 1912, pp. 2-7.)

THE TRANSMISSION OF TO DOG THROUGH SERRATICEPS.

LEISHMANIA FROM DOG THE AGENCY OF PULEX

IN the first experiment a dog which had been under observation for a period of two months, during which time it had appeared perfectly healthy, was subjected on eighty-two occasions to the bites of fleas which had previously been fed upon infected dogs.

Rather more than a month later evidence of infection appeared, and the animal lost condition. Examination of marrow from the femur, however, proved negative. Subsequently the animal's condition improved, and it was then killed. ,

At the post-mortem parasites in an apparently degenerated condition were found in small numbers in preparations from the spleen and bone-marrow.

Cultures on the Novy-M'Neal-Nicolle medium yielded numerous lepto­monads by the eighth day. This strain had up to the time of writing been passed through thirteen subcultures.

Two other animals, one of which was bitten daily for fifteen days by a single flea fed upon an infected dog every two days during the preceding fortnight, and the other which was bitten daily for four days by a flea which had had a single meal on an infected dog two days previously, failed to become infected.

In the intestinal contents of fleas that had been fed upon infected dogs, and in two out of nine fleas fed upon supposedly healthy dogs, numerous resting forms of the parasite were observed. These possessed a fairly large nucleus and blepharoplast.

In the f<ecal matter and in the posterior portion of the intestine slender bi-nucleated flagellates were discovered measuring 10 to 12 microns in length. Transitional forms were also observed. (Sergent (Ed.), Sergent (Et.),Lheritier, and Lemaire, Bull. Soc. Path. Exot., Vol. V., No.8, October 19 12, pp. 595-597·)

HUMAN TUBERCLE BACILLI IN THE MILK OF A VACCINATED COW.

AFTER referring at some length to the investigations of Weber and Titze, and to some of the results obtained by the Royal Commission, the author states that recently the opportunity offered to test the milk of two heifers that were vaccinated with human tubercle bacilli as calves. In the milk of one of these tubercle bacilli of the human type were found.

The animal was vaccinated when four days old, the inoculation being performed intravenously, and presumably with a minimal dose. She calved