Toxicologie de nanoparticules métalliques à de faibles concentrations, pertinentes du point de vue environnemental CNRS, UMR EPOC 5805 Université de Bordeaux.

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Toxicologie de nanoparticules mtalliques de faibles concentrations, pertinentes du point de vue environnemental CNRS, UMR EPOC 5805 Universit de Bordeaux Station marine dArcachon par Jean-Paul Bourdineaud professeur, Universit de Bordeaux 1 er CONGRS SANT-ENVIRONNEMENT Organis par lURPS-Mdecins libraux de PACA 6 & 7 Dcembre 2013 Marseille - Palais du Pharo Slide 2 Comment des nanoparticules peuvent-elles tre introduites dans un systme cologique ? Quelle peut tre lampleur de la pollution ? Slide 3 Kaegi et al., Environmental Pollution, 156 (2008) 233-239 Fresh facade painting Older facade painting Collected particles from runoff events Concentration of micro and nanoparticles in rain runoffs Slide 4 Les nanoparticules peuvent-elles tre distribues dans les diffrents compartiments dun systme cologique ? Et si oui, quel est le niveau de transfert vers les diffrentes composantes biologiques ? Slide 5 (Ferry et al.) A single dose, 7.1 x 10 11 of gold nanorods/L (65 x 15 nm), making 157 g Au NPs/L. Slide 6 Genotoxic impact of metal nanoparticles on the zebrafish Danio rerio Gold nanoparticles are used in pregnancy tests, toothpaste, energizing creams and beverages, detection and treatment of cancer tumours. Cadmium sulfide nanoparticles are used in electronic devices. MesoGold, 20 ppm colloidal gold, 250 mL, by Purest Colloids Price: $29.97 Fulfilled by Amazon (Gift-wrap). Supports mental focus & concentration, and improved hand/eye coordination. Promotes improved memory & a general feeling of well being. Slide 7 Impact of cadmium sulfide and gold nanoparticles on the zebrafish Controls CdS (10 nm)CdS (50 nm) Au (12 nm)Au (50 nm) Contamination through diet (5 g nanoparticles/g food) giving 40 ng nanoparticles/fish/day Exposure length: 60 days 100 nm Nanoparticles embedded in the food 12 nm NPs 50 nm NPs Slide 8 Metal quantification (cadmium and gold) in zebrafish tissues after 60 days of exposure. Gold could not be quantified in tissues and even in whole fish. Detection limits by atomic absorption spectrometry : Cadmium : 0,1 g/L Gold : 1 g/L Diet LiverBrainMuscles Whole fish Control food 8 89 0,90,6 0,1 32 8 CdS (10 nm) 348 3019 31,8 1 170 10 CdS (50 nm)364 3010 33,1 1 50 5 Liver is the organ accumulating the most cadmium The little nanoparticles are more accumulated than the bigger in the brain and at the scale of the whole fish. Total Cd in ng/g Slide 9 Differential gene expression after 60 days of exposure sod1sod2mt 2rad 51c-junp53baxpyccox1 Liver Au (12 nm)1/181/3811/711/101/271/451/201/91/12 Au (50 nm)/1/28//1/71/161/4// CdS (10 nm)/1/712331/6/1/2/1/4 CdS (50 nm)1/31/23 /1/6/1/5 / Brain Au (12 nm) 233238208261/9 Au (50 nm) /5//56/41/38 CdS (10 nm) /225//2/1/5/ CdS (50 nm) 333/10/27/ Muscle Au (12 nm)////1/2//9/ Au (50 nm)///1/33/ 1/61/16 CdS (10 nm)51020326124// CdS (50 nm)/0,4/2////1/15 Oxidative stressDetoxication DNA repair General stress Mitochondrial metabolism 1/ CdS and gold nanoparticles trigger a different gene expression pattern. 2/ Size does matter ! Slide 10 Control DNA Contaminated DNA Pollutant Probe OPB OPB Loss of hybridization site Principle of RAPD (Random amplified polymorphic DNA) Slide 11 OPB Principle of RAPD (Random amplified polymorphic DNA) OPB Control DNA Creation of hybridization site Contaminated DNA Slide 12 Quantification of genotoxic damages by RAPD-PCR methodology RAPD probeTmoinsCdS (10 nm)CdS (50 nm)Au (12 nm)Au (50 nm) OPB7 0,6 0,10,7 0,1* 6,5 0,9* 8,5 0,9* 1,9 0,2 OPB11 (2,1 0,7).10 -5 * (31 14).10 -5 * (4300 1800).10 -5 * (5600 1800).10 -5 * (280 70).10 -5 1/ The numbers of hybridization sites for RAPD probes was modified by nanoparticles. 2/ The two metals presented different effects. 3/ Size still matters ! Genomic DNAs were extracted from whole zebrafish individuals Number of hybridization sites per genome of the RAPD probe RAPD probes sequences: OPB7 : 5-GGTGACGCAG-3 OPB11 : 5-GTAGACCCGT-3 n = 10, mean SEM Slide 13 Les nanoparticules piges dans les sdiments aquatiques peuvent-elles tre libres dans la colonne deau et reprsenter un risque pour la sant des animaux ? Slide 14 Impact of gold nanoparticles (14 nm) on zebrafish exposed to a spiked sediment Brain AChE activity (nmol/mg/min) Brain ache gene expression (relative to bactin gene) 440 17546 8551 22 0.08 0.010.10 0.010.33 0.05 ControlsAu (16 mg/kg)Au (55 mg/kg) Exposition : 20 days 0.25 g/L0.8 g/L Slide 15 Les nanoparticules incluses dans les sols peuvent-elles reprsenter un risque pour la sant des animaux ? Slide 16 Metal nanoparticles trigger an oxidative stress in the earthworm Eisenia fetida Silver nanoparticles are used as antibacterial and antifungal agents in textiles, water treatments, and medical devices. Slide 17 Impact of gold and silver nanoparticles on the red earthworm Contamination through the soil Exposure length: 10 days ControlsGold NPsIonic gold Silver NPsIonic silver 2, 10, and 50 mg/kg Slide 18 Lipid peroxidation and protein oxidation in earthworms exposed for 10 days to ionic and nanoparticular god and silver Lipid peroxidationProtein carbonylation MDA : malondialdehyde CH 2 CC H H O O Slide 19 Counter-adaptive levels of catalase in earthworms exposed for 10 days to ionic and nanoparticular gold and silver

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