UTB 8449: Thomas Reinard, Molekularbiologische Methoden - Leseprobe

7/23/2019 UTB 8449: Thomas Reinard, Molekularbiologische Methoden - Leseprobe

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2/17

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3/17

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4/17

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5/17

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6/17

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7/17

3

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8/17

2

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9/17

8P} dhmyma F}ib

Ho dhmyma P}ykaamobkoc smvdmo shv }oy k}ibaht dmo Umltgvmo fmyibthcmo "Lk~htmj 5(/ Dkyyhod ymjfyt!vm~jhphmvmodm DOK!Agjmljm* ho dhmmho vmadmy DOK!Evkcamot mhocmymtpt smvdmolkoo* dkaht yhm my ho mhom om}m Shvtypmjjm fvho!cmo/ Dhm fmlkootmytmo Umltgvkvtmo yhod ^jky!ahdm/ K} dhmym Smhym motytmbmo tvkoycmom Gv!ckohyamo* smjibm d}vib dhm K}okbam vmadmvDOK om}m Mhcmoyibktmo mvbkjtmo bkfmo* fmh!y~hmjysmhym ytmjjmo yhm mho om}my ^vgtmho bmv/Mhohcm dhmymv Gvckohyamo yhod v agjml}jkv!fhgjgchyibm Kvfmhtmo fmygodmvy c}t cmmhcomt}od smvdmo kjy Agdmjjgvckohyamo fmpmhibomt/^vgd}phmvt mho tvkoycmomv Gvckohya}y mho vma!dmy ^vgtmho* y~vhibt ako ugo bmtmvgjgcmv Mr!~vmyyhgo/ Dhm K}vmhohc}oc }od Kokjxym ygjibmvbmtmvgjgcmv ^vgtmhom hyt Cmcmoytkod dmv Lk~h!

tmj 2 }od 8/ Dmv Okibsmhy fmythaatmv ^vgtm!hom fmyibvolt yhib bhmv k} Kothlv~mv!fkyhmvtmOkibsmhyumvkbvmo shm Haa}ovf}oc }odMJHYK "Lk~htmj ;>(/ Ho Pmhtmo dmv Cmoga~vg`mltmohaat dhm Hogvakthgoyy}ibm fmv dky Hotmvomtmhomo smymotjhibmo Tmhj dmv Kvfmhtypmht mhomyAgjml}jkvfhgjgcmo ho Koy~v}ib* smybkjf ho Lk!~htmj ;; p}ahodmyt mho l}vpmv Mhofjhil ho dhmymoTbmamofmvmhib p} odmo hyt/ Ho dhmyma Lk~htmj

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D}vibbv}oc ugo Jkfgv p} Jkfgv/ Dhmy fmdm}tmt*dkyy mhom ho dhmyma F}ib fmyibvhmfmom Amtbgdmambv gdmv smohcmv ytkvl umvodmvt d}vibcmbvtsmvdmo lkoo/ Dkbmv yhod dhm k}cmjhytmtmo ^vg!tglgjjm kjy Ugvyibjkc p} fmtvkibtmo }od ohibt kjyytvhltm Kvfmhtykojmht}oc/ Yhm s}vdmo k}cmoga!amo }a dhm p}cv}odmjhmcmodmo ^vhoph~hmo p}umvdm}tjhibmo* dmoo jmtptmodjhib pbjt o}v mhomYkibm0 Mhom Amtbgdm ygjjtm }olthgohmvmo/


10/17

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11/17

;;

; Dk Jmfmood mhom Fmkodmhjm

;/; Dm Kefk uo DOK od vOK

;/6 Dm cmomhibm Idm

;/9 Dhm Cmom

;/4 Dhm ^mhom

;/= Dhm pmjjm


12/17

Dky Jmfmo }od ymhom Fmytkodtmhjm;6

K} dmo mvytmo Fjhil mvyibmhot dky Jmfmo k} dmvMvdm mrtvma uhmjjthc/ Yibgo ho dmv ljmhoytmofhgjgchyibmo Mhobmht* dmv Pmjjm* odmo shv lga!~jmrm ytv}lt}vmjjm }od }olthgokjm P}ykaamo!

bocm/ K} dmo psmhtmo Fjhil jkyymo yhib kjjcm!amhom ^vhoph~hmo motdmilmo* dhm my mvjmhibtmvo*Cmamhoykalmhtmo pshyibmo dmo uhmjjthcmo fhg!jgchyibmo Ugvcocmo p} motdmilmo0;/ Kjjm fhgjgchyibmo Yxytmam yhod bhmvkvibhyib

ytv}lt}vhmvt/6/ My chft ho fhgjgchyibmo Yxytmamo mhom ymbv

mocm Umvlo~}oc pshyibmo dmv Ytv}lt}v }oddmv E}olthgo/

Cmvkdm mhoakj uhmv umvyibhmdmom Kvtmo ko Ak!lvgagjmljmo yhod v dhm mogvam Uhmjkjt umv!kotsgvtjhib/ Ho dhmyma F}ib fmkyymo shv }oyaht dmo O}ljmhoy}vmo }od dmo ^vgtmhomo/ ^g!jxykiibkvhdm }od Jh~hdm ytmjjmo mfmokjjy shib!thcm Aklvgagjmljm dkv* smvdmo bhmv `mdgibohibt fmbkodmjt/Kjjm Aklvgagjmljm yhod bhmvkvibhyib k}cm!fk}t* dmoo yhm fmytmbmo k}y Agogamvmo* kjygljmhomvmo Agjmljmo/ Dhm Agogamvm smvdmofmv mhom Lgodmoykthgo }otmv Kfy~kjt}oc ugoSkyymv p} haamv cvmvmo Mhobmhtmo p}yka!

amocmct0 O}ljmgthdm smvdmo p} O}ljmhoy}!vmo lgodmoyhmvt* Kahogy}vmo p} ^m~thdmo }od^vgtmhomo/ Ygjibm Aklvgagjmljm loomo d}vibBxdvgjxym shmdmv ho ljmhomvm Mhobmhtmo* fhy bhop} dmo Agogamvmo pmvjmct smvdmo/Bm}tm hyt kjjcmamho klpm~thmvt* dkyy Vhfgo}ljm!hoy}vmo "VOK( dhm jtmytmo Fhgagjmljm dkv!ytmjjmo/ Mhomvymhty loomo yhm ho hbvmv Ym{}mopcmomthyibm Hogvakthgomo y~mhibmvo* shm dhmy

ho VOK!Uhvmo dmv Ekjj hyt* kodmvmvymhty yhod yhmk} uhmjjthcm Smhym mopxakthyib klthu* shm hoVhfgygamo gdmv Y~jhimgygamo/ Y~tmv ho dmvMugj}thgo bkfmo yhib kodmvm Fhgagjmljm k}

`msmhjy mhom dmv fmhdmo E}olthgomo y~mphkjhyhmvt/Dhm Dmygrxvhfgo}ljmhoy}vm "DOK( hyt ibmahyibuhmj ytkfhjmv }od }ochmvt kjy Y~mhibmv dmv cmomth!yibmo Hogvakthgo* ^vgtmhom dhmomo ygsgbj dmvYtv}lt}vfhjd}oc* kjy Y~mhibmv~vgtmhom ygshm kjyLktkjxyktgvmo ho dmv Pmjjm/^vhoph~hmjj umvj}t dmv cmomthyibm Hogvakth!goye}yy ho mhom Vhibt}oc0 ugo dmv DOK fmvdhm aVOK kjy Pshyibmoyt}m bho p}a ^vgtmho/Dhmymv }ohumvymjjm Hogvakthgoye}yy shvd Pmo!tvkjmy Dgcak dmv Agjml}jkvfhgjgchm cmokoot"Kff/ ;/;(/ Dhm cmomthyibm Hogvakthgo shvdd}vib uhmv umvyibhmdmom* ythilytgbkjthcm Fkymoho dmv DOK cmy~mhibmvt0 dmo }vhomo Kdmoho "K(}od C}koho "C( ygshm dmo ^xvhahdhomo Ixtgyho"I( }od Tbxaho "T(/ Dky ]ayibvmhfmo dhmymvcmomthyibmo Hogvakthgo ugo DOK k} aVOKbmht Tvkoylvh~thgo/ K}ib dhm dkv p}ytodhcmaVOK o}tpt v dhm Hogvakthgoyumvahttj}ocuhmv umvyibhmdmom Fkymo* sgfmh ytktt Tbxahobhmv ]vkihj "]( umvsmodmt shvd/ Ha psmhtmo

Yibvhtt shvd dhm Hogvakthgo dmv aVOK ho mhom^vgtmhoym{}mop }acmyibvhmfmo* dhmymv ^vgpmyyshvd Tvkoyjkthgo cmokoot/ Fmvmhty Kokoc dmv3>mv @kbvm s}vdm mhom K}yokbam dhmymy }oh!umvymjjmo Dgcaky motdmilt* dmoo Vmtvguhvmoyhod ho dmv Jkcm* hbvm k} VOK cmy~mhibmvtm Ho!gvakthgo ho DOK }ap}yibvmhfmo/ Dkbmv s}vdmdky Pmotvkjm Dgcak }a dhmym Acjhiblmht mv!smhtmvt/

DOK VOKTvkoylvh~thgo ^vgtmhoTvkoyjkthgo

vmumvym Tvkoylvh~thgoVm~jhlkthgo

Kff/ ;/;0Pmotvkjmy Dgcak dmv Agjml}jkvfhgjgchm/ D}vib dhm Vm~jhlkthgo dmv DOK shvd dhm cmomthyibm Hogvakthgo umvuhmjjthct }odk} dhm Tgibtmvpmjjmo smhtmvcmcmfmo/ Dmv cmomthyibm Hogvakthgoye}yy j}t ugo dmv DOK fmv dhm VOK p}a ^vgtmho/ D}vibdhm Motdmil}oc dmv Vmumvymo Tvkoylvh~thgo ho Vmtvguhvmo s}vdm dky Lgopm~t moty~vmibmod mvsmhtmvt/


13/17

;9Dmv K}fk} ugo DOK }od VOK

Mhom smhtmvm pmotvkjm Ugvk}yymtp}oc v dkyJmfmo ygjj bhmv ohibt }omvsbot fjmhfmo0 dhm cm!omthyibm Hogvakthgo a}yy vmcmjahc d}~jh!phmvt smvdmo "Kff/ ;/;(/ Dhmymo ^vgpmyy omootako Vm~jhlkthgo }od mv hyt moc umvlo~t ahtdmv Tmhj}oc dmv Pmjjm/ D}vib dhm ugvbmvhcm D}!~jhlkthgo dmv cmomthyibmo Hogvakthgo shvd cm!sbvjmhytmt* dkyy fmhdm Tgibtmvpmjjmo okib mhomvPmjjtmhj}oc dhm cjmhibm cmomthyibm Hogvakthgo

mvbkjtmo/

;/; Dm Kefk uo DOK od vOK

Ho kjjmo Jmfmsmymo fmytmbt dky cmomthyibm Ak!tmvhkj k}y O}ljmhoy}vmo/ Dhmym ytmjjmo gjxamvmk}y mhomv ymbv ljmhomo Pkbj }otmvyibhmdjhibmvAgogamvm dkv* dmo O}ljmgthdmo/ Dhm O}ljmg!thdm shmdmv}a fmytmbmo k}y dvmh umvyibhmdm!omo Lga~gomotmo0 mhoma P}ilmv }od mhoma^bgy~bkt* dhm dky Vilcvkt dmv O}ljmhoy}vm

fhjdmo* ygshm mhomv ythilytgbkjthcmo Fkym* dhmdmo mhcmotjhibmo Hogvakthgoytvcmv dkvytmjjt"Kff/ ;/6(/Dhm mhopmjomo O}ljmgthdm yhod fmv =9 ^bgy!~bgdhmytmvfhod}ocmo ahtmhokodmv umvlo~t*sgd}vib dky Agjmlj mhom fmythaatm K}yvhib!t}oc k}smhyt0 uga vmhmo =!^bgy~bktmodmbho p}a vmhmo 9!GB!Modm "Kff/ ;/9(/ KjjmDOK!agdhphmvmodmo Mopxam shvlmo ko dhmymv

^bgy~bgdhmytmvfhod}oc* d/ b/ DOK gdmv VOK!Agjmljm smvdmo p}oibyt ko dmv bgy~bgdhmy!tmvfhod}oc lgodmoyhmvt "1jhchmvt* kjyg p}yka!amocmct( gdmv bxdvgjxyhmvt "cmy~kjtmo(/Sbvmod P}ilmv }od ^bgy~bkt v dhm ytv}l!t}vmjjm Hotmcvhtt dmv DOK ygvcmo* hyt dhm dvhttmLga~gomotm* dhm ythilytgbkjthcmo Fkymo* vdhm Igdhmv}oc dmv cmomthyibmo Hogvakthgo umv!kotsgvtjhib/ Ho dmv DOK yhod dhmy dhm fmhdmo }!vhom Kdmoho "K( }od C}koho "C( ygshm dhm xvh!ahdhom Ixtgyho "I( }od Tbxaho "T(/ Fmh VOKshvd Tbxaho d}vib ]vkihj "](* mfmokjjy mho ^x!vhahdho* mvymtpt/

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Kff/ ;/60Dhm DOK hyt k}y dvmh umvyibhmdmomo Lga~gomotmo k}cmfk}t0 mhomv ugo uhmv umvyibhmdmomo Fkymo "Kdmoho* C}koho* Ixtgyhogdmv Tbxaho(* dhm ko mhomo P}ilmv* "mhom Dmygrxvhfgym( cmf}odmo yhod/ Dhmym Lgafhokthgo k}y Fkym }od P}ilmv shvd kjy Dmy!grxo}ljmgyhd fmpmhibomt/ Kfbochc ugo dmv Fkym mvchft yhib yg Dmygrxkdmogyho* !ixthdho* !c}kogyho }od !tbxahdho/ D}vib dhm

Fhod}oc mhomy Agog~bgy~bkty ko dmo P}ilmv motytmbt mho Dmygrxo}ljmgthd* smjibmy kjjcmamho kjy dOA^ kfcmlvpt shvd/Ev dhm umvyibhmdmomo Fkymo mvchft yhib dkbmv Dmygrxkdmogyhoagog~bgy~bkt* "dKA^( mti/ Evmhm* ohibt mhocmfk}tm O}ljmgthdmjhmcmo kjy momvchmvmhibm Tvh~bgy~bktm ugv* smjibm kjjcmamho kjy dOT^ }od `m okib Fkym p/F/ Dmygrxc}kogyhotvh~bgy~bkt "dCT^(cmokoot smvdmo/ Fmha Mhofk} smvdmo dhmym dOT^y dm~bgy~bgvxjhmvt* ygdkyy Agog~bgy~bktm "dOA^( motytmbmo/ Ha Cmcmo!yktp p}v DOK tvct VOK mhomo kodmvmo P}ilmvkotmhj* mhom Vhfgym/ Dkbmv jjt fmh VOK haamv dhm Ugvyhjfm Dmygrx smc "p/F/Ixtgyhoagog~bgy~bkt* IA^(/


14/17


15/17

;=Dmv K}fk} ugo DOK }od VOK

Kff/ ;/40Dg~~mjytvochcm DOK fmytmbt haamv k}y dmo ^kkvmo K }odT fps/ I }od C }od fhjdmt mhom vmibtydvmbmodm Bmjhr k}y/Ho dhmymv Ytv}lt}v fhjdmo yhib kjtmvohmvmod mhom p}coc!

jhibm* cvgm E}vibm \Ak`gv CvggumQ ygshm mhom ljmhom E}vibm\Ahogv CvggumQ k}y/

Kff/ ;/=0@m okib Cmbkjt ko CI!^kkv}ocmoytmhct dhm Yibamjptma~m!vkt}v mhomv DOK ko "K(/ DhmDmokt}vhmv}oc ugo dyDOKlkoo ~bgtgamtvhyib fmh mhomvSmjjmojocm 165> oa umvgjctsmvdmo "F(/ Dhm Tma~mvkt}v* fmhdmv dhm Bjtm dmv DOK Agjmljmmhopmjytvochc ugvjhmcmo* bmhtYibamjptma~mvkt}v TA/ Ho dmvKffhjd}oc jhmct dmv Ta fmh 29 I/

sky p} mhomv Tvmoo}oc dmv DOK Ytvocm bvt/Dhmymo Ugvckoc omoot ako Dmokt}vhmv}oc dmvDOK/ DOK dmokt}vhmvt fmh Tma~mvkt}vmo psh!yibmo 3> ! 8> I/ Dhm Tma~mvkt}v* fmh dmv dhm

Bjtm dmv DOK Ytvocm mhopmjytvochc ugvjhmct*shvd Yibamjptma~mvkt}v "Ta( cmokoot/ Dk dhmK!T!^kkv}oc psmh Skyymvytgfvilmo k}yfhjdmt

}od I!C dvmh* hyt dhm Yibamjptma~mvkt}v ohibto}v ugo dmv Jocm dmv DOK Agjmljm kfbochc*ygodmvo k}ib ugaK!T! fps/ C!I!Cmbkjt/ Akolkoo dhm DOK!Dmokt}vhmv}oc ~bgtgamtvhyib"Lk~htmj 9/2( okibumvgjcmo "Kff/ ;/=(* dmooyyDOK kfygvfhmvt dky Jhibt ytvlmv kjy dyDOK/Dhmymy Umvbkjtmo shvd Bx~gibvgamv Mmlt cm!okoot }od ho Lk~htmj 9/2/; mhocmbmodmv fmbko!dmjt smvdmo/Dhm Dmokt}vhmv}oc dmv DOK hyt vmumvyhfmj* skyfmdm}tmt* dkyy yhib fmh Kflbj}oc shmdmv dhmfmhdmo DOK!Ytvocm p}ykaamojkcmvo/ DhmymvUgvckoc dmv Vmokt}vhmv}oc hyt ytkvl kfbochcugo dmv Jocm dmv fmhdmo DOK!Agjmljm/ Fmhl}vpmo DOK Agjmljmo ugo mhohcmo Tk}ymodFkymo j}t dhmymv Ugvckoc hoomvbkjf ugo Ah!o}tmo vmibt yibomjj }od mphmot kf* v DOKYtvocm ugo fmv ;>4 ! ;>= Fkymo smvdmo Yt}o!

dmo fhy Tkcm fmothct/Dmv K}fk} dmv VOK hyt dma dmv DOK ymbv bo!jhib/ D}vib dhm Vhfgym ha Vilcvkt hyt dhm Ytv}l!t}v dmv VOK emrhfjmv kjy dhm dmv DOK/ Smhtmvbhoodmt yhib ho dmv VOK ytktt Tbxaho dky ^xvhah!dho ]vkihj "]( "Kff/ ;/5(/Mhcmotjhib hyt VOK mhopmjytvochc* yhm tmodhmvtkfmv dkp}* mfmokjjy dg~~mjytvochcm K}kjt}o!

Ljmhom

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9*4 oa

6 oa


16/17

Dky Jmfmo }od ymhom Fmytkodtmhjm;5

cmo d}vibp}bvmo/ Dhmym dg~~mjytvochcmo Fm!vmhibm smvdmo o}v fmv vmjkthu l}vpm Ym{}mop!kfyibohttm k}ycmfhjdmt* smhj dmv koth~kvkjjmjm^kvtomvytvkoc mbjt/ Dhmym dyVOK!Vmchgomosmvdmo cmfhjdmt* smoo psmh Kfyibohttm k} dmacjmhibmo Agjmlj gdmv k} psmh umvyibhmdmomoVOK!Agjmljmo p}mhokodmv lga~jmamotv yhod/Yhm bkfmo mhom mogvam fhgjgchyibm Fmdm}t}oc*dmoo ha Cmcmoyktp p}v DOK lkoo VOK d}vibhbvm emrhfjm Ytv}lt}v k}ib mopxakthyibm E}ol!

thgomo fmvombamo;/My chft dvmh shibthcm Kvtmo ko VOKy0 Dhm vhfgygakjm VOK "vVOK( ho dmo Vhfgyg!

amo akibt }ocmbv 2= - dmv cmykatmo VOKmhomv Pmjjm k}y }od ytmjjt ugv kjjma dhm mopx!akthyibm Lga~gomotm dmv Vhfgygamo dkv/

Dhm Tvkoymv!VOK "tVOK( lgaat k} }ocm!bv ;> - dmv Cmykat!VOK }od hyt mfmokjjyko dmv Tvkoyjkthgo fmtmhjhct/ Yhm umvahttmjtpshyibmo dmo Igdgoy dmv aVOK }od dmo

p}cmbvhcmo Kahogy}vmo/ Dhm tVOK fmyhtptmhohcm ymjtmom Fkymo }od }ocmsbojhibm Fk!ymo~kkv}ocmo/ Yhm fhjdmt ymbv ibkvkltmvhyth!yibm Ytv}lt}vmo k}y/

Dhm Amyymocmv!VOK "aVOK( fmvtvct dhmYm{}mophogvakthgo ugo dmv DOK k} dmoTvkoyjkthgoyk~~kvkt }od akibt smohcmv kjy= - dmv Cmykat!VOK k}y/

; Lktkjxthyib klthum VOKy smvdmo kjy Vhfgpxam fmpmhib!omt }od yhod ho dmv Okt}v smht umvfvmhtmt* p/F/ ho Vhfgyg!amo }od Y~jhimgygamo/

;/6 Dm cmomhibm Idm

Dk dmo uhmv Fkymo dmv DOK fps/ aVOK psko!phc umvyibhmdmom Kahogy}vmo v dhm ^vgtmhomcmcmofmvytmbmo* fhjdmo haamv dvmh k}mhoko!dmvgjcmodm Fkymo mhom Hogvakthgoymhobmht vmhom fmythaatm Kahogy}vm/ Dhm DOK!Kfgjcm

KTIshvd yg fmh dmv Tvkoylvh~thgo hoK]I }acm!yibvhmfmo }od koyibjhmmod ho dhm Kahogy}vm

Hygjm}iho tvkoyjkthmvt/D}vib dhm Umvsmod}oc ugo Tvh~jmtty loomoakrhakj 54 umvyibhmdmom Lgafhokthgomo mot!ytmbmo "4r4r4 Ukvhkotmo 1 54(* cmo}c }a dhmpskophc Kahogy}vmo p} igdhmvmo/ Dkbmv lo!omo mhohcm Kahogy}vmo d}vib umvyibhmdmom Tvh!~jmtty* dhm ygcmokootmo Igdgoy* vm~vymothmvtsmvdmo "Kff/ ;/3(/ Gt }otmvyibmhdmo yhib Ig!dgoy* dhm v dhm cjmhibm Kahogy}vm igdhmvmo*ho dmv dvhttmo Fkym/ Fmhy~hmjysmhym igdhmvmoCIK*

CII* CIC }od CIT kjjm v dhm Kahogy}vm Kjk!oho "Kff/ ;/3(/ Ako y~vhibt ho dhmyma P}yka!amobkoc uga dmcmomvhmvtmo Igdm* fmh dma dhmdvhttm Fkym mhomy Igdgoy ukvhhmvmo lkoo "sgf!fmjo \tg sgffjm 1 skilmjo* yibskolmoQ(/

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Dk haamv dvmh Fkymo mhom Kahogy}vmvm~vymothmvmo* lkoo ako k}y dmv Jocm mhomvDOK!Ym{}mop k} dhm }ocmbvm Cvm dmy p}cm!bvhcmo ^vgtmhoy yibjhmmo0 Mhom DOK!Ym{}mopugo 8>> Fkymo~kkvmo "f~( igdhmvt v mho vgtmhok}y 9>> Kahogy}vmo/ Kjy Ek}ytvmcmj lkoo v

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}od shvd ho VOK d}vib dhm Fkym ]vkihj"F( mvymtpt/


17/17

;3Dhm Cmom

mhom Kahogy}vm mho d}vibyibohttjhibmy Agjm!l}jkvcmshibt ugo ;;> Dk kocmogaamo smvdmo/Dhm 8>> f~ DOK igdhmvmo kjyg v mho vgtmho k}y9>> Kahogy}vmo aht }ocmbv 99 lDk/ Dhmy hyt

o}v mhom cvgfm Kfyibtp}oc* dmoo mhopmjom Kah!ogy}vmo smhibmo ho hbvma Agjml}jkvcmshibtytkvl kf* k}mvdma yhod ^vgtmhoagdhlkthgomo*shm Cjxlgyxjhmv}ocmo* ohibt fmvilyhibthct/

Dvmh smhtmvm Igdgoy igdhmvmo ohibt v mhom Kah!ogy}vm/ Dhmym Igdgoy dhmomo kjy Ytg~~igdgoy*dmoo fmh hbomo fvhibt dhm Tvkoyjkthgo kf/

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Dhm Cv}odfk}ytmhom* smjibm DOK* VOKgdmv ^vgtmhom k}fk}mo* yhod ho kjjmo Jmfmsm!ymo cjmhib/ K}ib dmv cmomthyibm Igdm hyt fhy k}smohcm K}yokbamo }ohumvymjj/ Dhmy chjt k}ibv pmjj}jvm ^vgpmyym shm Tvkoylvh~thgo* Tvkoy!jkthgo gdmv ~vhavm Ytgsmibymj/Ugo dhmymo kjjcmamhomo ^vhoph~hmo chft my o}vsmohcm K}yokbamo/ Fmhy~hmjysmhym umvj}t dmv

Hogvakthgoye}yy fmh Vmtvguhvmo ugo dmv VOKp}v DOK/ Mhohcm Ahtgibgodvhmo }od smohcm ohm!dmvm M}lkvxgotmo o}tpmo kfsmhibmodm Igdgoyv fmythaatm Kahogy}vmo/ Mhohcm ^vgtmhom

motbkjtmo fmygodmvm Kahogy}vmo/Dhmym }ohumvymjjmo ^vhoph~hmo mvacjhibmo mvytdhm agjml}jkvfhgjgchyibmo Kvfmhtytmibohlmo*shm shv yhm bm}tm lmoomo/ Gbom dhmym lootmlmho ^vgtmho ho mhoma kvtvmadmo Gvckohya}ybmvcmytmjjt smvdmo/ Dhmy hyt k}ib mho dm}tjhibmvBhosmhy dkvk}* dkyy dky Jmfmo k} dhmymv Mvdmo}v mho mhophcmy Akj motytkodmo hyt/

;/9 Dhm Cmom

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Documents

UTB 8449: Thomas Reinard, Molekularbiologische Methoden - Leseprobe