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ZymoPURE™: One Technology for all of your Plasmid NeedsEmpower your research with ZymoPURE™ plasmid DNA purification kits. Streamlined methodology and superior technology enables unrivaled speed and performance. Innovative binding technology enables DNA to be purified using a microcentrifuge column via vacuum or centrifuge in as little as 15 minutes. Ultra-pure, transfection-grade plasmid DNA is eluted directly from the spin-column into a 1.5 ml microcentrifuge tube and is ready for sensitive downstream applications.
Innovation. Pure & Simple.™
Innovative, PURE™ technology matrix
unique column design enables elution from a microcentrifuge (Mini, Midi, Maxi)
unique 45° angle prevents retention of contaminants
Luer Lock cap facilitates vacuum or centrifuge-based processing
Featuring the ZymoPURE™ Midiprep Spin-Column
• 25x higher binding capacity• orders of magnitude better
endotoxin removal• the lowest elution volumes
From E. coli
Miniprep Scale
ZymoPURE™ Plasmid Miniprep Kit
Rapid purification of ≤ 100 μg transfection-grade plasmid
DNA
Midiprep, Maxiprep, and Gigaprep Scales
ZymoPURE™ Kits ZymoPURE-EndoZero™ ZymoPURE-Express™
Transfection-ready plasmid DNA in ≤18 minutes, using
a spin-column: Midi ≤ 300 μg
Maxi ≤ 1200 μgGiga ≤ 10 mg
Ultra-low endotoxin levels (≤ 0.025EU/ug) in under
20 minutes
Pellet free isolation. Culture to transfection
in only 15 minutes. No large-scale centrifugation
ZymoPURE™ Plasmid Miniprep KitHighlights
• Fast and reliable purification of up to 100 µg of transfection-grade plasmidDNA using a spin-column.
• Innovative ZymoPURE™ technology enables elution of ultra-pure endotoxin-free plasmid DNA in as little as 25 µl.
Superior YieldsTransfection-grade
Supplier Q Supplier MNM ZymoPURE™
than the industry leading miniprep kit
50,000 timesfewer ENDOTOXINS
Product Cat. No. Size
ZymoPURE™ Plasmid Miniprep Kit
D4208T 10 preps.
D4209 50 preps.
D4210 100 preps.
D4211 400 preps.
D4212 800 preps.
Streamlined, Innovative Workflow
rapid loading onto a spin-column via vacuum or centrifuge
bindfor ultra-pure endotoxin free plasmid DNA
washtransfection ready plasmid DNA
elute
Plasmid DNA yield and concentration from the ZymoPure™ Miniprep Kit compared to other major suppliers. Plasmid DNA (pGL3®) was isolated from 5 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol (in duplicate).
ZymoPURE™ Plasmid Midiprep, Maxiprep, Gigaprep KitsHighlights
• Fast, easy, reliable, ultra-pure transfection-ready plasmid DNA directly froma spin column.
• Innovative ZymoPURE™ binding technology enables elution of the highestconcentration of plasmid DNA directly from a spin-column using amicrocentrifuge.
Highest Recovery100 times less endotoxins using ZymoPURE™
ZymoPURE™
Maxiprepavg: 0.0197 EU/µg DNA
Supplier Q Maxiprep avg: 2.5435 EU/µg DNA
Endotoxin Units/µg DNA
0 1 2.50.5 21.5 3
Product Cat. No. Size
ZymoPURE™ Plasmid Midiprep KitD4200 25 preps.
D4201 50 preps.
ZymoPURE™ Plasmid Maxiprep KitD4202 10 preps.
D4203 20 preps.
ZymoPURE™ Plasmid Gigaprep Kit D4204 5 preps.
Supplier Q1 Supplier Q2M
ZymoPURE™
Plasmid DNA yield and concentration from the ZymoPURE™ Maxiprep Kit compared to other major suppliers. PlasmidDNA (pGL3®) was isolated from 150 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol (in duplicate). One (1) µl of eluted plasmid DNA was visualized post agarose gel electrophoresis. M, ZR 1 kb DNA Marker (Zymo Research).
Endotoxin levels of plasmid DNA isolated using the ZymoPURE™ Maxiprep kit compared to maxiprep kits from Supplier Q. Plasmid (pGL3®) was isolated from 150 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protcol (in duplicate).
Streamlined, Innovative Workflow
rapid loading onto a spin-column via vacuum or centrifuge
bindfor ultra-pure endotoxin free plasmid DNA
washtransfection ready plasmid DNA
elute
ZymoPURE-EndoZero™ Plasmid KitsHighlights
• Consistently achieve endotoxin levels of ≤ 0.025 EU/µg of plasmid DNA inonly 20 minutes.
• Plasmid DNA is ideal for transfection in sensitive cells and in vivo research.• The unique spin-column based procedure purifies up to 1.2 mg of endotoxin-
free plasmid DNA.
Streamlined, Innovative Workflow
Achieve the Lowest Endotoxin Levels for the Most Sensitive Applications
Featuring EndoZero™ Column Technology
Simply use the EndoZero™ spin-column to remove
additional endotoxins from the ZymoPURE™ eluate
Endotoxin removal in minutes!
ZymoPURE™ technology paired with Zymo’s proprietary EndoZero technology has set a new industry standard for endotoxin levels, with its ultra endotoxin-free EndoZero™ columns.
Supplier QEndoFree
ZymoPURE™ - EndoZero
Supplier Q
Endo
toxi
n U
nits
/µg
Plas
mid
DN
A
FDA Limit for Vaccines< 0.04 EU/µg DNA
0.000
0.025
0.050
0.075
0.100
2
4
6
8
10
Product Cat. No. Size
ZymoPURE-EndoZero™ Plasmid Maxiprep Kit D4205 10 preps.
ZymoPURE-EndoZero™ Plasmid Gigaprep Kit D4207 5 preps.
Endotoxin levels in plasmid DNA isolated using the ZymoPURE-EndoZero™ Maxiprep kit from Zymo Research. Plasmid DNA (pGL3®) was isolated from 150 ml ofJM109 E. coli culture grown overnight following the manufacturer’s suggested protocol (in duplicate). Endotoxin Concentration was measured in the eluted plasmid DNA using the chromogenic Pyrochrome® endotoxin specific assay from Associates of Cape Cod Incorporated. Shown are means ± SD of 2 preps.
bindrapid loading onto a spin-column via vacuum or centrifuge
washfor ultra-pure endotoxin-free plasmid DNA
transfection ready plasmid DNA
elutefor removal of additional endotoxin via centrifuge
filter
Highlights
• Innovative pellet-free procedure bypasses the standard cell-pelleting andresuspension steps. No centrifugation required!
• The fastest, easiest, most reliable method for purification of up to 1.2 mg ofultra-pure endotoxin-free plasmid DNA.
• Plasmid DNA is eluted directly from a microcentrifuge column and is well-suited for transfection and other sensitive applications.
ZymoPURE-Express™ Plasmid Midiprep Kit
Streamlined, Innovative WorkflowNo large-scale centrifugation
Superior Yield The Fastest Method for Plasmid Purification
neutralizeusing Zymo’s patented color buffer system
rapid loading onto a spin-column via vacuum or centrifuge
bind/washtransfection ready plasmid DNA
elutelysedirectly from culture without cell pelleting
Plasmid DNA yield and concentration from the ZymoPURE Express™ Midiprep Kit compared to other major suppliers. Plasmid DNA (pGL3) was isolated from 25 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol in duplicate. The eluted plasmid DNA was visualized post agarose gel electrophoresis. M, ZR 1 kb DNA Marker (Zymo Research).
Save up to 100 minutes with the ZymoPURE™-Express Midiprep Kit.
Plasmid DNA yield and concentration from the ZymoPURE – Express™ Maxiprep kit compared to other major suppliers. Plasmid DNA (pGL3) was isolated from 25 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol in duplicate. The eluted plasmid DNA was visualized post agarose gel electrophoresis. M, ZR 1 kb DNA Marker (Zymo Research).
MZymoPURE™ Supplier Q1 Supplier Q2 Supplier S Supplier P Supplier MN
Product Cat. No. Size
ZymoPURE-Express™ Plasmid Midiprep Kit D4213 25 preps.
Zymo Research
Supplier Q
Supplier MN
Supplier P
Supplier I
Supplier T
Supplier S
0
20
40
60
80
100
120
Proc
essi
ng T
ime
(min
)
Only 15 Minutes!
*Ampicillin selection only
Related Products
Zyppy® Plasmid Kits
Mix & Go Competent Cells
Zymoclean™ Gel DNA Recovery Kits
Product Cat. No. Size
Zymoclean™ Gel DNA Recovery Kit (uncapped)
D4001 50 preps
D4002 200 preps
Zymoclean™ Gel DNA Recovery Kit (capped)
D4007 50 preps
D4008 200 preps
ZR-96 Zymoclean™ Gel DNA Recovery KitD4021 2 x 96 preps
D4022 4 x 96 preps
Zymoclean™ Large Fragment DNA Recovery Kit
D4045 25 preps
D4046 100 preps
DNA fragments recovered from an agarose gel using the Zymoclean™ Gel DNA Recovery Kit. Lanes: M: DNA Ladder; 1-5: individual ladder DNA fragments.
M 1 2 3 4 5
23 kb -9 kb -
2 kb -
500 bp -
Boost recoveries from agarose gels to > 80%
Pellet-free plasmid preparations
Mix & GoCompetent Cells
Others
Place on Ice
Spin to Concentrate Cells
Remove SupernatantAdd SOC
20 second* transformations!
Ice 45 min.
EcoRI digestion of plasmid DNA (pGEM®) isolated from E. coli culture using the Zyppy™ Plasmid Miniprep Kit or the QIAprep™ Spin Miniprep kit from Qiagen. The amount of DNA loaded was standardized based on culture volume input. Performed in triplicate.
1 kb Zymo Research Supplier Q Product Size Cat. No.
Zyppy™ Plasmid Miniprep Kit50 Preps. D4036
100 Preps. D4019
Zyppy™-96 Plasmid Miniprep Kit2 x 96 Preps. D4041
4 x 96 Preps. D4042
Zyppy™-96 Plasmid MagBead Miniprep2 x 96 Preps. D4100
4 x 96 Preps. D4101
Zyppy™ Plasmid Midiprep Kit25 Preps. D4025
50 Preps. D4026
Strain 10 x 100 µl 95 x 50 µl
Zymo 5α(DH5α) T3007 T3009
JM109 T3003 T3005
Zymo 10B(DH10B) T3019 T3020
TG1 T3017 -
42°C 2 min. (Heatshock)
Make Your Own Size Cat. No.
Mix & Go ! E. coli Transformation Kit 20 ml T3001
Mix & Go ! E. coli Transformation Buffer Set 60 ml T3002
Service Technique (réactifs et instrumentation) : 01 34 60 60 24 - [email protected] Service Commande - Clients : 01 34 60 15 16 – [email protected]
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