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Apoptosis
Definition
Causes / Significance
Morphology
Mechanism / Cellular events
Clinical significance
Definition
1. Pathway of cell death induced by a tightly regulated
intracellular program in which cells destined to die,
activate enzymes that degrade the cells own nuclear
DNA & nuclear & cytoplasmic proteins.
Definition:
2. Internally programmed cell death.
3.“A form of cell death, designed to eliminateunwanted host cells through activation ofcoordinated, internally programmed series ofevents effected by a dedicated set of geneproducts”.
Causes/significance
PHYSIOLOGICAL
PATHOLOGICAL
PHYSIOLOGICAL SITUATIONS:
Embryogenesis.
Hormone dependent involution in adults.
Cell deletion in proliferating populations.
Cell death by cytotoxic T cells.
Elimination of harmful self reactive lymphocytes.
Death of host cells that have served their useful purpose.
PATHOLOGICAL CONDITIONS
Injurious stimuli
Cell injury in viral diseases
Pathological atrophy in parenchymal organs after
duct obstruction
Cell death in tumours
MORPHOLOGY
Cell shrinkage
Chromatin condensation
Formation of cytoplasmic blebs & apoptotic
bodies
Phagocytosis of Apoptotic cell by macrophages
HISTOLOGICAL EXAMINATION
H&E STAIN
Apoptotic cell appears as an round/oval mass of
intensely eosinophilic cytoplasm with dense
nuclear chromatin fragments.
MECHANISM/CELLULAR EVENTS
1. SIGNALING
2. CONTROL & INTEGRATION
3. EXECUTION
4. REMOVAL OF DEAD CELLS
1. SIGNALING
Transmembrane signals
-suppress preexisting death programs
-initiate a death cascade
Tumour necrosis factor receptor (TNFR):
oligomerize leading to activation of initiator caspases & a
cascade of enzyme activation culminating in cell death.
2. CONTROL & INTEGRATION
Direct transmission of death signals by specific
adapter proteins to the execution mechanism.
Regulation of mitochondrial permeability by
members of BCL-2 family of proteins.
-mitochondrial permeability transition
Cytochrome c – apoptotic trigger
Cytochrome c binds to cytosolic proteins (e.g.,proapoptotic
protease activating factor/Apaf-1) & activates them triggering
execution caspases activation.
BCL-2 suppresses apoptosis by preventing increased
mitochondrial permeability.
Other members of BCL-2 family bind to BCL-2 &
modulate its antiapoptotic effect, thus BCL-Xl
inhibits apoptosis while BAX & BAD promote
programmed cell death.
3. EXECUTION
Protein cleavage
DNA breakdown
Protein cross-linking
Phagocytic recognition
Protein cleavage
Activation of caspases
-caspases – cleave cellular proteins (lamins) leads to break
up of nuclear scaffold & cytoskeleton
-activate DNase which degrade nuclear DNA.
DNA breakdown:
DNA breakdown into oligonucleosomes in multiples of 180-300
base pairs by Ca2+ & Mg2+ dependent endonucleaes.
DNA fragments identified by a technique agarose gel
electrophoresis.
Apoptosis-regular fragmentation associated with activation of
p53 gene.
Necrosis- “smeared” pattern.
Protein cross-linking
Via transglutaminase activation
-converts soluble cytoplasmic proteins into
covalently condensed shell leading to formation
of apoptotic bodies.
4. Phagocytic recognition
Apoptotic cells express phosphatidyl serine in the outer
layers of plasma membrane ‘flipped’ from the inner layers.
Some express thrombospondin an adhesive glycoprotein
Other proteins secreted by phagocytes may bind to
apoptotic cells & opsonize the cells for phagocytosis.
Tumours – apoptotic index as a measure of
proliferation
Apoptosis in tumours increases following irradiation &
immune responses.
Measurement of apoptosis in-vivo/ in-vitro following
treatment may predict effectiveness of therapy.
CLINICAL SIGNIFICANCE