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Presented by A.O. Warsame, P.M. Kimani and J.W. Mwangi (Department of Plant Science and Crop Protection, College of Agriculture and Veterinary Sciences, University of Nairobi) at the First Bio-Innovate Regional Scientific Conference, Addis Ababa, Ethiopia, 25-27 February 2013
Citation preview
Variation and Selection for Cooking time in Drought tolerant
Canning Beans (Phaseolus vulgaris)
Warsame, A. O., P. M. Kimani, and J.W. MwangiDepartment of Plant Science and Crop Protection, College of
Agriculture and Veterinary Sciences, University of Nairobi
University of Nairobi ISO 9001:2008 1 Certified http://www.uonbi.ac.ke
First Bio-Innovate Regional Scientific ConferenceUnited Nations Conference Centre (UNCC-ECA)Addis Ababa, Ethiopia, 25-27 February 2013
Contents
Introduction Materials and Methods Results &Discussion Conclusions Acknowledgements
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Introduction Common beans is one of most import crops in eastern Africa
(Pachico, 1993; Kimani, 2001) Cooking time of dry bean is an important trait related to its
utilization (Iyer et al., 1980; Miles and Sonde, 2004; Elia, 2003). Bean cooking time can vary from ½ hour to 8 hours (Miles and
Sonde, 2004). In Kenya, traditional bean varieties need 2-3 hours to cook (Kimani,
2001). Cooking time is import for both canning industry and household
consumption. For processors, long cooking is associated with higher energy
requirements and lower processor yields (Van Loggerenberg, 2004).
Urban consumers spend more money on expensive fuels. Rural households use large quantities of firewood and spend
time in searching it.University of Nairobi ISO 9001:2008 3 Certified http://www.uonbi.ac.ke
Continued…… Thus, faster-cooking bean cultivars would: save energy and deliver higher processor yield for canning
industry save money spent on kerosene, charcoal, electricity, and natural
gas for or rural and urban consumers. Save forests. However, at present there is a little information on fast cooking
bean varieties in the region. Processing industry in Kenya depend on single variety (Mex-142)
which is highly susceptible to rust and anthracnose, and is low yielding.
Therefore, there is need for new faster cooking bean varieties which combine other important traits like disease resistance, drought tolerance and other agronomic traits, and also meet consumer preferences for seed colors.
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The objective of this study was to evaluate the cooking time of advanced canning bean lines previously selected for drought tolerance, resistance to diseases and other agronomic traits
Objectives
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Material and Methods
Bean seeds used in this study were obtained from Grain Legume Research Program of the University of Nairobi, which fall in 7 market classes.
Mex142 was used as check 155 Bean lines of diverse market classes
were grown and selected at Kabete Field station, University of Nairobi during the 2012 long-rain season season.
Seeds were soaked for 16 hours and cooked using Matson Bean cooker (Matson, 1946) and trial was repeated three times.
Analyses of variance (ANOVA) appropriate to a randomized complete-block design was performed using GenStat 13.3 (VSN International Ltd., UK.) at significance level (0.01).
Market class Number of lines
Red mottled 22
Red kidneys 23
Speckled Sugars
22
Pintos and Cariocas
22
Small reds 22
Navys 24
Mixed Colors 20
Total 155
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Continued….
Red mottled
Red kidney
Speckled sugar
Navy
Small red
Pinto
Carioca
Yellow
Tan
Selection in the field
Matson Bean Cooker
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Soaking
Results
Analysis of variance showed that there were highly significant (P<0.01) genotypic differences for cooking time within and among market classes.
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Line
BCB 11-
130
BCB11-1
32
BCB11-1
33
BCB11-1
35
BCB11-1
43
BCB11-1
44
BCB11-1
45
BCB11-2
90
BCB11-3
05
BCB11-3
14
BCB11-3
24
BCB 11-
334
BCB11-3
45
BCB 11-
347
BCB 11-
400
BCB 11-
413
BCB 11-
430
BCB 11-
433
BCB 11-
445
BCB 11-
464
BCB 11-
470
BCB 11-
523
MEX 1
420
5
10
15
20
25
30
35
40
45
50
0
46.342.9
34.3
41.9
33.1
38.940.7
31.834.9 36.1
38.342.1
35.8
41.9
31.4
36.3
30.633
43
32.2
40.7
34.4
41.6
Fig 1. Cooking time of advanced Red Mottled canning bean lines selected at Kabete Field Sta-
tion, 2012
Genotype
Min
ute
s
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Mean 37.48LSD (0.05) 2.801SE 0.567CV (%) 1.5
BCB 11-158
BCB 11-159
BCB 11 -162
BCB 11-163
BCB 11-166
BCB 11-168
BCB 11-171
BCB 11-173
BCB 11-174
BCB 11-175
BCB 11-176
BCB 11-196
BCB 11-285
BCB 11-325
BCB 11-327
BCB 11-337
BCB 11-342
BCB 11-468
BCB 11-492
BCB 11-500
BCB 11-503
BCB 11-509
BCB 11-522
MEX 142
0
5
10
15
20
25
30
35
40
45
50
55
31.3
41.6
48.8
39.136.6 37.8
32.6
39.5
46.3
38.7 38.7
32.1 32.135.2
27.131.1 31.8
40.9
35.6
41.4 43
29.6 27.8
42.4
Fig 2. Cooking time of advanced Red Kidney canning bean lines selected at Kabete Field Sta-
tion, 2012
Line
Min
ute
s
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Mean 36.77LSD (0.05) 2.973SE 0.265CV (%) 3.7
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BCB11
-204
BCB11
-312
BCB11
-393
BCB11
-530
BCB11
-209
BCB11
-317
BCB11
-414
MEX
142
BCB11
-217
BCB11
-336
BCB11
-426
BCB11
-233
BCB11
-380
BCB11
-495
BCB11
-269
BCB11
-383
BCB11
-501
BCB11
-282
BCB11
-386
BCB11
-507
BCB11
-303
BCB11
-390
BCB11
-508
0
10
20
30
40
50
60
35.334.87
37.639.03
32.4333.430.4
41.1
51.53
29.67
35.27
41.73
34.73
43.27
33.7331
38.7
30.13
32.17
39.7
36.1
50.83
30.43
Line
Min
ute
s
Fig 3. Cooking time of advanced speckled sugars canning bean lines
selected at Kabete Field Station, 2012
Mean 36.66LSD (0.05) 3.797SE 0.033CV (%) 2.1
BCB 11-10
BCB 11-108
BCB 11-14
BCB 11-30
BCB 11-34
BCB 11-35
BCB 11-355
BCB 11-40
BCB 11-47
BCB 11-476
BCB 11-49
BCB 11-52
BCB 11-55
BCB 11-58
BCB 11-62
BCB 11-63
BCB 11-69
BCB 11-75
BCB 11-80
BCB 11-87
BCB 11-9
BCB 11-94
BCB 11-98
MEX 142
0.0
10.0
20.0
30.0
40.0
50.0
60.0
41.7
32.2
49.8 48.9
41.3
48.9
43.3
39.3
33.831.8
35.3
39.3
24.4
34.030.4
32.0
40.5
35.9 34.636.3 36.4
29.6
37.9
42.0
Fig 4. Cooking time of advanced Navy Bean lines selected at Kabete Field Station, 2012
Line
Min
ute
s
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Mean 37.21LSD(0.05) 3.836SE 2.337CV(%) 1.5
BCB 11-
182
BCB 11-
184
BCB 11-
191
BCB11-1
92
BCB 11-
193
BCB 11-
194
BCB 11-
195
BCB 11-
197
BCB 11-
199
BCB 11-
202
BCB 11-
245
BCB 11-
280
BCB 11-
323
BCB 11-
331
BCB 11-
344
BCB 11-
362
BCB 11-
366
BCB 11-
399
BCB 11-
401
BCB 11-
437
BCB 11-
443
BCB 11-
517
MEX 1
420.0
10.0
20.0
30.0
40.0
50.0
60.0
38.7
31.6
27.1
33.7 32.5 32.0
40.8
28.731.2
41.8
35.4 35.439.6
34.5
29.8 30.6
35.3
42.3
52.4
35.136.9 38.3
40.5
Fig 5. Cooking time of advanced Small Red canning bean lines selected at Kabete Field Station, 2012
Line
Min
ute
s
Mean 37.21LSD(0.05) 3.836SE 2.337CV(%) 1.5
Results of this work showed significant variation in cooking among bean lines across market classes.
More than 60 lines from different market classes cooked faster than the industry standard Mexican-142.
This genetic variation for cooking time, can combined with drought tolerance, resistance to diseases and other agronomic traits.
Such lines will save time, energy for rural and urban poor in cities and processors.
A broad range of Consumer preferences can be met.
Increased utilization of beans and income for farmers.
Conclusions
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The University of Nairobi Bio-Innovate-Africa program Beans for Climate Change Consortium Supervisors Bean Program Technical staff-UON Governments of Kenya, Sweden-Sida and Somalia
Acknowledgements
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University of Nairobi ISO 9001:2008 16 Certified http://www.uonbi.ac.ke
THANK YOU