Diagnostic de laborator în Micoplasmoza Respiratorie Aviară (MRA)

Def.

Dg clinic

Dg clinic

Dg clinic

Dg clinic

Dg clinic

lesions classic for MG infection including sinusitis, nasal discharge, and adhesion of the eyelids caused by conjunctivitis.

Dg anatomopatologic

Dg anatomopatologic

Dg anatomopatologic

Dg anatomopatologic

Dg anatomopatologic

Mycoplasma hyosinoviae

Dg anatomopatologic

Izolare si identificare Mycoplasma gallisepticum

Dg serologic – probe ser de pasare (Ac-anti

Mycoplasma)

Dg de laborator

Pasari bolnave, cadavre, organe cu

leziuni

Examen bacterioscopic +

bacteriologic

Examen histopatologic

Examen imunologic (Ifd)

Bioproba

Reactia de hemaglutinare

Probe de sange, ser

Ev cronicaEv enzooticaTulb respLez caracteristice

Date epidemiologice

RSAR + RAL

Detectare Ac

antimicoplasma

Examen bacterioscopic + bacteriologic

Examen bacterioscopic - Coloratie Giemsa cu fixator Bouin

-Dificil de interpretat (detritusuri celulare + leziuni cronice – paucibacilare

Examen bacteriologic – medii speciale PPLO bulion si agar

Examen histopatologic

Izolatele se pot verifica prin:

teste imunologice – Ifd

tehnici de biologie moleculara - PCR

Examen bacteriologic

Dg serologic

Detectare Ac anti Mycoplasma RSAR +

RAL

Ac anti Mycoplasma = ? in serurile de

cercetat

Ag = suspensie de micoplasme

inactivate

RSAR+

+

RAL

Ag 1,8 ml +Ser 0,2 ml 1/1

0

Ag 1 ml Ag 1 ml Ag 1 ml Ag 1 ml Ag 1 ml

1/20

1/40

1/80

1/160

1/320