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Diagnostic de laborator în Micoplasmoza Respiratorie Aviară (MRA). Def. Dg clinic. Dg clinic. Dg clinic. Dg clinic. Dg clinic. Dg anatomopatologic. lesions classic for MG infection including sinusitis, nasal discharge, and adhesion of the eyelids caused by conjunctivitis. - PowerPoint PPT Presentation
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Def.
Dg clinic
Dg clinic
Dg clinic
Dg clinic
lesions classic for MG infection including sinusitis, nasal discharge, and adhesion of the eyelids caused by conjunctivitis.
Dg anatomopatologic
Dg anatomopatologic
Dg anatomopatologic
Dg anatomopatologic
Dg anatomopatologic
Mycoplasma hyosinoviae
Dg anatomopatologic
Izolare si identificare Mycoplasma gallisepticum
Dg serologic – probe ser de pasare (Ac-anti
Mycoplasma)
Dg de laborator
Pasari bolnave, cadavre, organe cu
leziuni
Examen bacterioscopic +
bacteriologic
Examen histopatologic
Examen imunologic (Ifd)
Bioproba
Reactia de hemaglutinare
Probe de sange, ser
Ev cronicaEv enzooticaTulb respLez caracteristice
Date epidemiologice
RSAR + RAL
Detectare Ac
antimicoplasma
Examen bacterioscopic + bacteriologic
Examen bacterioscopic - Coloratie Giemsa cu fixator Bouin
-Dificil de interpretat (detritusuri celulare + leziuni cronice – paucibacilare
Examen bacteriologic – medii speciale PPLO bulion si agar
Examen histopatologic
Izolatele se pot verifica prin:
teste imunologice – Ifd
tehnici de biologie moleculara - PCR
Examen bacteriologic
Dg serologic
Detectare Ac anti Mycoplasma RSAR +
RAL
Ac anti Mycoplasma = ? in serurile de
cercetat
Ag = suspensie de micoplasme
inactivate
RSAR+
+
RAL
Ag 1,8 ml +Ser 0,2 ml 1/1
0
Ag 1 ml Ag 1 ml Ag 1 ml Ag 1 ml Ag 1 ml
1/20
1/40
1/80
1/160
1/320