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Hull et. al. 2015. 43
SUPPLEMENTAL FIGURE LEGENDS: Supplemental Figure 1: Western blots used to generate the densitometry values depicted in Figure 3.
Supplemental Figure 2: Western blots used to generate the densitometry values depicted in figure 6. Supplemental Figure 3: Densitometry measurements for the blots depicted in Figure 7. Bars depict the mean ± SEM of measurements normalized to GAPDH. Data are expressed in arbitrary units. P values < 0.05 using ANOVA and the Newman-Keuls post-test were considered significant. n = 3-4/group. Supplemental Figure 4: Transmission electron micrographs of left ventricle (LV) from wild-type and cardiac-specific overexpression of HO-1 (cs-HO-1) mice treat with vehicle or 14 or 60 days after treatment with doxorubicin. Micrographs were captured at 1650x total magnification. Scale bar represents 2 µm. n = 3 – 5 mice per group, with at least 5 images evaluated per mouse. Images were assessed by a reviewer blinded to the different groups.
B-Actin
COX3
ND1
NRF1
PGC1α
TFAM
Polγ
cs-HO-1cs-HO-1WT WT
Vehicle DOX
SUPP FIGURE 1
B-Actin
Fis1
Mfn1
Mfn2
DRP1
GAPDH
WT cs-HO-1 WT cs-HO-1Vehicle DOX
B
cs-HO-1cs-HO-1WT WT
Vehicle DOX
A
SUPP FIGURE 2
SUPP FIGURE 3
SUPP FIGURE 4
Wild-Type cs-HO-1Ve
hicl
eD
ay 1
4D
ay 6
0
A B
D
E
C
F
Cropped Figure 7
cs-HO-1cs-HO-1
HO-1
PINK1
GAPDH
32 kDa
63 kDa
37 kDa
~50 kDa
WTcs
HO-1 WT WT
Vehicle DOX Day 5 DOX Day 14
Pon S
B
Figure 7 Original Blot – no lanes croppedLanes:cc12cc34567891011121314
Lanes:1234567891011121314
HO-1
PINK1
GAPDH
Pon S
Full uncropped gel for Figure 7
c = lanes cropped from the image of the blot in figure 7.Numbered lanes were incorporated into figure 7.The 4th, and 5th cropped lanes were superfluous, depicting resultsfrom cs-HO-1 mice already shown in lane 2. The 2nd croppedlane was the incorrect genotype. The 1st cropped lane wassuperfluous, showing the same control as lane 1.
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