Identifikasi ke LIPI Pembuatan Simplisia Maserasi dengan Etanol 80

78

Lampiran 1. Hasil Identifikasi tumbuhan

Lampiran 2. Alur Penelitian

Identifikasi ke LIPI

Pembuatan Simplisia

Maserasi dengan Etanol 80%

Diuapkan dengan rotary evaporator

Tumbuhan

Ekstrak Etanol

P1 P2 P3 P4

Parameter:

BB/minggu

Pakan/hari

Air minum/hari

Analisis:

Protein kasar

Lemak kasar

Total Kolesterol

Diberikan melalui air minum/hari

Setelah 35 hari perlakuan

79

Lampiran 3. Hasil Analisis Kadar Protein Dan Lemak

80

Lampiran 3. (lanjutan)

81


82

Lampiran 4. Hasil Analisis Kolesterol total

83

Lampiran 5. Perhitungan Kadar Lemak Kasar

No. Berat labu awal

(g)

Berat labu lemak

(g)

Berat sampel

(g)

1. 142,9547 143,2135 1,0282

2. 143,9887 144,2497 1,0275

3. 153,9255 154,1843 1,0268

1.

2.

3.

% Rata-rata lemak kasar = 25,26%3

25,20%25,40%25,17%

84

Lampiran 6. Perhitungan Kadar Protein

% Kadar protein = 37812,4x(g)sampelberat

blanko) volume- titrasi(volume

No. Berat sampel (g) Volume titrasi

(ml)

Volume (ml)

1. 0,0565 0,290 0,05

2. 0,0549 0,280 0,05

3. 0,0543 0,280 0,05

1. Kadar protein = %59,1837812,4x0,0565

,050-0,290

2. Kadar protein = %34,1837812,4x0,0549

,050-0,280

3. Kadar protein = %54,18100%x0,0543

,050-0,280

% Rata-rata kadar protein = ,46%183

,54%18,34%1818,59%

85

Lampiran 7. Bagan Alur Pengambilan Serum Darah Ayam

Broiler

Sayap belakang bersih

Darah ayam

Serum siap diukur

Dipangkas bulu bagian sayap

belakang

Dibilas dengan alkohol

Diambil darah dengan syringe

pada bagian vena axillaris

Didiamkan selama 10 menit

Disentrifugasi dengan kecepatan

3000 rpm selama 15 menit

Diambil cairan bening

86

Lampiran 8. Bagan Alur Pengukuran Kadar Kolesterol

Serum

Dipipet sebanyak 10 µl

Dimasukkan dalam tabung reaksi

yang berisi larutan reagensia

kolesterol sebanyak 1 ml

Dihomogenkan

Diinkubasi pada suhu kamar

selama 20 menit

Diukur pada mikrolab 300 dengan

panjang gelombang 546 nm

Dicatat hasilnya

Kadar kolesterol total

87

Lampiran 9. Bagan Alur Penetapan Kadar Protein Metode Mikro Kjeldahl

0,05 g sampel daging ayam

Tambahkan 1g Selenium dan 3 tetes H2O2 dan 2,5 ml H2SO4 pekat

Dimasukkan dalam tabung reaksi

Destruksi di ruang asam hingga jernih

didinginkan

Diencerkan dengan H2O 50 ml aquades, dikocok masukkan ke labu kjehldal

Didestilasi

Sampel yang diencerkan dengan aquades dimasukkan 10 ml ke labu kjehldal

Tambahkan penolphtalein 3 tetes dan NaoH 50% sampai larutan menjadi merah

Destilat ditampung dalam erlenmeyer berisi

5 mL Asam Borat 3%, aquades 25 ml dan 2 tetes indicator hingga larutan yang

mengandung uap Nitrogen menjadi 150 ml

Destilat dititrasi dengan HCl 0,01 N

Kadar Protein dihitung dengan blanko 0,05

X

88

Lampiran 10. Bagan Alur Penetapan Kadar Lemak

Sampel

Ditimbang sebanyak 1 g, dibungkus dengan kertas

saring dan diikat

Dimasukkan dalam tabung Soxhlet berisi

pelarut n-heksana

Labu penampung diisi pelarut n-heksana

50 ml Dipasangkan pada alat ekstraksi soxhlet, dihidupkan

Diamati perubahan warna, selama perebusan sampel

Setelah jernih alat ekstraksi dimatikan

Bahan direbus, sampai labu boiling tampak jernih

atau sampai lemak tidak terlihat lagi

Dicatat hasilnya

Sampel dikeringkan dalam oven 105 C selama 12 jam

Kadar Lemak

89

Lampiran 11. Data Penimbangan bobot badan ayam perminggu

Jenis

Perlakuan

Pengamatan (minggu)

awal 1 2 3 4 5

P0

1 211.75 555.8 1701.5 3411.5 4750.45 7501.25

2 202.25 471.45 1530.35 3420.5 4811.45 7471

3 201.75 500.95 1561.1 3441 4891.45 7570.85

rata 205.25 509.4 1597.65 3424.333 4817.783 7514.367

sd 5.634714 42.80517 91.24148 15.11897 70.71304 51.20098

P1

1 201.25 490.7 1730.35 3626 5331.5 8076.05

2 206.25 530.85 1711.6 3665.45 5381.1 7941

3 210.75 490.85 1741.75 3632 5430.9 7731.75

rata 206.0833 504.1333 1727.9 3641.15 5381.167 7916.267

sd 4.752192 23.13743 15.22358 21.25718 49.70003 173.4775

P2

1 221.5 521.03 1746.1 3696 4115.7 7931.25

2 216.25 475.95 1690.5 3695 5190.5 7761

3 221 447.25 1685.45 3641 5121.2 7881.5

rata 219.5833 481.41 1707.35 3677.333 4809.133 7857.917

sd 2.897556 37.19181 33.65334 31.46956 601.5297 87.54082

P3

1 221 575.85 1751.3 4042.1 5700.4 7871.65

2 211.75 555.65 1841.1 3670.75 5660.3 8050.95

3 212.25 565.55 1800.9 3741 5830.1 8096.25

rata 215 565.6833 1797.767 3817.95 5730.267 8006.283

sd 5.202163 10.10066 44.98192 197.2718 88.7526 118.7755

P4

1 226.75 590.43 1750.45 3906.25 5730.75 8271.1

2 226.5 595.45 1735.55 3940.5 5960.6 8486.2

3 216.25 496 1795.35 3841.25 6061.15 8415.75

rata 223.1667 560.6267 1760.45 3896 5917.5 8391.017

sd 5.991313 56.02459 31.12893 50.41267 169.3642 109.6622

90

Lampiran 12. Analisis Statistik Bobot Badan Ayam

Test of Homogeneity of Variances

Levene

Statistic df1 df2 Sig.

Awal .750 4 10 .580

minggu1 2.376 4 10 .122

minggu2 3.543 4 10 .048

minggu3 8.111 4 10 .004

minggu4 9.642 4 10 .002

minggu5 1.082 4 10 .416

One-Sample Kolmogorov-Smirnov Test

15 15 15 15 15 15

213.8167 524.2507 1718.2233 3691.3533 5331.1700 7937.1700

8.52385 46.66340 81.91611 185.55469 530.27525 307.26071

.134 .158 .211 .162 .133 .102

.113 .158 .143 .157 .084 .102

-.134 -.149 -.211 -.162 -.133 -.082

.518 .612 .818 .629 .514 .396

.952 .849 .515 .824 .955 .998

N

Mean

Std. Deviation

Normal Parametersa,b

Absolute

Positive

Negative

Most Extreme

Differences

Kolmogorov-Smirnov Z

Asymp. Sig. (2-tailed)

awal minggu1 minggu2 minggu3 minggu4 minggu5

Test distribution is Normal.a.

Calculated from data.b.

91


ANOVA

Sum of

Squares df Mean Square F Sig.

Awal Between Groups 765.808 4 191.452 7.616 .004

Within Groups 251.375 10 25.138

Total 1017.183 14

minggu1 Between Groups 16501.354 4 4125.339 2.950 .075

Within Groups 13983.264 10 1398.326

Total 30484.618 14


Within Groups 25363.392 10 2536.339

Total 93943.479 14


Within Groups 86256.808 10 8625.681

Total 482027.577 14

minggu4 Between Groups 3124946.50

7 4 781236.627 9.624 .002

Within Groups 811739.322 10 81173.932

Total 3936685.82

9 14

minggu5 Between Groups 1188702.44

6 4 297175.611 22.340 .000

Within Groups 133025.583 10 13302.558

Total 1321728.02

9 14

92


awal

Duncana

3 205.2500

3 206.0833 206.0833

3 215.0000 215.0000

3 219.5833

3 223.1667

.843 .054 .086

PerlakuanPo

P1

P3

P2

P4

Sig.

N 1 2 3

Subset for alpha = .05

Means for groups in homogeneous subsets are displayed.

Uses Harmonic Mean Sample Size = 3.000.a.

minggu1

Duncana

3 481.4100

3 504.1333 504.1333

3 509.4000 509.4000

3 560.6267

3 565.6833

.402 .089

PerlakuanP2

P1

Po

P4

P3

Sig.

N 1 2




minggu2

Duncana

3 1597.6500

3 1707.3500

3 1727.9000

3 1760.4500

3 1797.7667

1.000 .067

Perlakuan

Po

P2

P1

P4

P3

Sig.

N 1 2




93


minggu3

Duncana

3 3424.3333

3 3641.1500

3 3677.3333 3677.3333

3 3817.9500 3817.9500

3 3896.0000

1.000 .644 .093 .328

PerlakuanPo

P1

P2

P3

P4

Sig.

N 1 2 3 4




minggu4

Duncana

3 4809.1333

3 4817.7833

3 5381.1667

3 5730.2667

3 5917.5000

.971 .052

PerlakuanP2

Po

P1

P3

P4

Sig.

N 1 2




minggu5

Duncana

3 7514.3667

3 7857.9167

3 7916.2667

3 8006.2833

3 8391.0167

1.000 .163 1.000

Perlakuan

Po

P2

P1

P3

P4

Sig.

N 1 2 3




94

Lampiran 13. Data Penggunaan Jumlah Pakan (gram)

Hari P0 P1 P2 P3 P4

1 2 3 1 2 3 1 2 3 1 2 3 1 2 3

1 30.8 31.1 33.3 16.1 15.9 18.0 26.3 26.0 28.0 29.7 29.1 31.2 22.7 23.0 24.6

2 55.0 55.6 59.4 42.0 41.3 46.8 49.2 48.6 52.3 33.2 32.5 34.8 46.8 47.5 50.7

3 98.9 99.8 106.8 90.4 89.0 100.9 83.6 82.5 88.9 94.1 92.3 98.8 95.2 96.8 103.2

4 115.0 116.1 124.2 101.7 100.1 113.5 96.7 95.5 102.8 113.9 111.7 119.5 96.9 98.5 105.0

5 120.0 121.2 129.6 103.2 101.6 115.2 105.0 103.7 111.6 112.2 110.0 117.8 106.5 108.3 115.4

6 123.0 124.2 132.8 112.9 111.1 126.0 114.8 113.3 122.0 113.9 111.7 119.5 119.5 121.5 129.5

7 123.0 124.2 132.9 132.3 130.2 147.6 128.0 126.3 136.0 122.2 119.8 128.2 125.8 127.9 136.3

8 126.2 127.5 136.3 140.4 138.2 156.7 127.9 126.2 135.9 125.5 123.0 131.7 132.3 134.5 143.4

9 145.8 147.2 157.5 140.3 138.1 156.6 128.1 126.5 136.2 138.8 136.1 145.6 141.9 144.3 153.8

10 181.3 183.1 195.8 164.5 161.9 183.6 154.1 152.1 163.8 181.6 178.1 190.6 161.3 163.9 174.8

11 255.7 258.2 276.2 235.6 231.8 262.9 245.9 242.7 261.4 244.2 239.5 256.3 201.6 205.0 218.5

12 257.4 260.0 278.1 240.3 236.5 268.2 237.8 234.7 252.8 260.8 255.7 273.6 203.3 206.6 220.3

13 275.2 277.9 297.2 258.1 254.0 288.1 259.2 255.8 275.5 287.2 281.6 301.4 232.4 236.2 251.9

14 283.2 285.9 305.9 261.3 257.1 291.6 270.5 267.0 287.6 297.0 291.3 311.7 238.7 242.7 258.7

15 323.7 326.9 349.7 266.2 262.0 297.0 272.1 268.6 289.3 298.8 293.0 313.5 267.9 272.3 290.3

16 297.9 300.8 321.8 266.2 262.0 297.1 278.8 275.1 296.3 305.4 299.5 320.5 271.0 275.5 293.7

17 307.6 310.6 332.3 267.9 266.2 296.4 287.0 283.3 305.0 306.9 301.0 322.1 279.0 283.6 302.4

18 343.1 346.5 370.6 272.6 270.8 301.6 308.3 304.3 327.7 318.6 312.5 334.4 313.0 318.1 339.2

19 353.0 356.5 381.3 271.0 269.3 299.8 341.0 336.6 362.4 331.7 325.2 348.1 322.7 328.0 349.7

20 364.1 367.6 393.3 277.5 275.7 307.0 357.4 352.8 379.9 371.4 364.1 389.7 351.6 357.4 381.0

21 414.3 418.3 447.5 293.6 291.7 324.8 393.5 388.4 418.3 420.9 412.7 441.7 345.3 351.0 374.2

22 420.8 424.9 454.5 313.0 311.0 346.3 396.7 391.6 421.7 419.1 411.0 439.9 380.9 387.1 412.7

23 449.9 454.3 486.0 348.5 346.3 385.6 408.3 403.0 434.0 425.9 417.6 447.0 393.5 400.0 426.5

24 456.3 460.8 492.9 419.4 416.7 464.0 416.5 411.1 442.7 432.3 423.9 453.7 419.4 426.2 454.4

25 491.9 496.7 531.4 422.7 420.0 467.6 431.1 425.6 458.3 437.5 429.0 459.1 440.5 447.7 477.3

26 550.2 555.6 594.3 438.8 436.0 485.5 436.1 430.5 463.6 448.9 440.2 471.1 454.8 462.3 492.9

27 553.5 558.9 597.9 441.9 439.1 489.0 436.1 430.4 463.5 457.2 448.3 479.8 459.7 467.2 498.1

28 555.0 560.5 599.5 442.1 439.3 489.1 439.4 433.7 467.1 465.3 456.3 488.3 471.1 478.9 510.5

29 556.8 562.3 601.4 467.7 464.7 517.5 449.3 443.4 477.5 470.3 461.2 493.5 483.9 491.8 524.3

30 561.2 566.7 606.2 471.0 468.0 521.2 462.3 456.3 491.4 477.0 467.7 500.6 490.5 498.5 531.5

31 563.2 568.7 608.4 482.3 479.2 533.6 467.4 461.3 496.8 491.8 482.2 516.1 490.3 498.4 531.3

32 568.1 573.7 613.7 485.6 482.5 537.3 478.8 472.6 508.9 501.7 492.0 526.5 500.1 508.3 541.9

33 572.2 577.8 618.1 487.1 484.0 538.9 487.0 480.7 517.7 511.6 501.6 536.8 512.9 521.3 555.8

34 579.4 585.0 625.8 495.2 492.0 547.9 492.0 485.6 522.9 521.5 511.4 547.3 516.3 524.8 559.5

35 586.0 591.7 633.0 506.6 503.4 560.5 506.7 500.2 538.6 531.4 521.0 557.6 524.2 532.8 568.0

95

Lampiran 14. Analisis Statistik Penggunaan jumlah pakan


15 15 15 15 15

635.4067 2117.8533 4383.7600 7534.5200 11172.55

37.01728 127.69000 294.80331 541.86560 810.70403

.159 .131 .121 .121 .112

.159 .131 .061 .121 .112

-.120 -.079 -.121 -.076 -.090

.618 .508 .469 .469 .435

.840 .959 .981 .980 .991

N

Mean

Std. Deviation


Absolute

Positive

Negative

Most Extreme

Differences



minggu1 minggu2 minggu3 minggu4 minggu5




1.034 4 10 .436

1.107 4 10 .405

.696 4 10 .612

.631 4 10 .652

.650 4 10 .640

minggu1

minggu2

minggu3

minggu4

minggu5

Levene


ANOVA

10143.069 4 2535.767 2.805 .085

9040.840 10 904.084

19183.909 14

127077.5 4 31769.369 3.140 .065

101188.8 10 10118.882

228266.3 14

811163.6 4 202790.896 5.000 .018

405562.3 10 40556.231

1216726 14

2938460 4 734615.061 6.267 .009

1172196 10 117219.634

4110657 14

6635064 4 1658765.878 6.464 .008

2566311 10 256631.079

9201374 14

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

minggu1

minggu2

minggu3

minggu4

minggu5

Sum of


96


minggu1

Duncana

3 613.6667

3 618.5333

3 625.3000

3 633.9333 633.9333

3 685.6000

.459 .062

PerlakuanP2

P1

P3

P4

Po

Sig.

N 1 2




minggu2

Duncana

3 1989.3000

3 2060.9000 2060.9000

3 2107.1667 2107.1667

3 2175.7667 2175.7667

3 2256.1333

.060 .051

PerlakuanP4

P2

P1

P3

Po

Sig.

N 1 2




minggu3

Duncana

3 4086.0000

3 4211.6333 4211.6333

3 4336.2667 4336.2667

3 4553.0000 4553.0000

3 4731.9000

.177 .075 .302

PerlakuanP1

P4

P2

P3

Po

Sig.

N 1 2 3




97


minggu4

Duncana

3 7006.5333

3 7332.2000

3 7349.9333

3 7670.1000

3 8313.8333

.051 1.000

Perlakuan

P1

P4

P2

P3

Po

Sig.

N 1 2




minggu5

Duncana

3 10515.30

3 10749.10

3 10967.60

3 11210.40

3 12420.33

.148 1.000

PerlakuanP1

P2

P4

P3

Po

Sig.

N 1 2




98

Lampiran 15. Data penggunaan Air Minum

ari P0 P1 P2 P3 P4

1 2 3 1 2 3 1 2 3 1 2 3 1 2 3

1 85.0 82.6 82.5 64.5 63.5 72.0 78.7 77.7 83.6 76.1 74.6 79.8 100.0 101.6 108.4

2 110.9 107.6 106.5 88.9 87.5 99.2 100.1 98.8 106.4 117.2 115.0 123.0 122.7 124.8 133.0

3 175.6 165.3 174.1 130.7 128.7 145.9 170.5 168.3 181.2 165.0 161.8 173.2 159.7 162.3 173.0

4 205.4 198.7 196.5 183.9 181.0 205.2 211.6 208.8 224.9 208.1 204.0 218.4 201.6 204.9 218.5

5 212.9 218.6 228.5 193.5 190.5 216.0 216.4 213.6 230.0 209.7 205.7 220.1 211.3 214.8 229.0

6 234.0 252.7 238.8 205.0 201.7 228.8 232.8 229.8 247.4 231.0 226.5 242.4 229.2 233.0 248.4

7 251.8 283.2 245.5 216.1 212.7 241.2 245.9 242.7 261.4 249.2 244.3 261.5 246.9 251.0 267.6

8 287.1 301.3 301.6 229.0 225.4 255.6 286.9 283.2 304.9 293.7 288.0 308.2 283.9 288.5 307.6

9 296.8 310.2 313.0 258.2 254.1 288.1 285.2 281.6 303.2 297.0 291.3 311.7 271.1 275.6 293.8

10 354.8 359.5 385.7 279.2 274.8 311.5 318.0 313.9 338.1 346.7 340.0 363.8 325.8 331.1 353.0

11 425.8 453.6 440.6 293.5 288.9 327.6 393.6 388.5 418.4 417.5 409.4 438.1 379.0 385.2 410.7

12 483.9 514.3 501.9 387.3 381.1 432.1 439.3 433.7 467.0 465.3 456.3 488.3 438.9 446.1 475.6

13 526.0 556.9 547.6 417.7 411.1 466.1 495.2 488.8 526.4 521.6 511.5 547.4 496.8 504.9 538.3

14 554.8 562.5 602.6 432.4 425.6 482.5 534.6 527.7 568.2 554.5 543.7 581.9 525.8 534.4 569.8

15 612.9 651.2 635.9 467.7 460.3 521.9 606.6 598.7 644.7 620.5 608.4 651.1 577.4 586.9 625.7

16 640.5 673.5 671.5 509.7 501.6 568.7 636.1 627.8 676.1 646.9 634.3 678.8 619.4 629.5 671.1

17 677.6 710.6 712.3 538.9 535.4 596.2 655.7 647.2 697.0 680.0 666.8 713.6 677.4 688.5 734.1

18 677.4 698.5 724.1 569.4 565.7 629.9 705.1 696.0 749.5 716.2 702.3 751.6 730.8 742.8 791.9

19 729.2 768.6 762.7 596.9 593.1 660.5 747.5 737.9 794.6 724.6 710.5 760.4 725.8 737.7 786.5

20 759.7 793.4 801.9 622.6 618.6 688.8 760.7 750.8 808.5 747.7 733.2 784.6 741.9 754.1 804.0

21 782.4 826.3 816.8 645.2 641.0 713.8 767.2 757.3 815.5 780.5 765.4 819.1 777.4 790.2 842.4

22 816.3 856.5 857.7 677.4 673.1 749.5 790.2 779.9 839.9 802.1 786.6 841.8 787.3 800.2 853.1

23 854.8 897.8 897.4 748.5 743.8 828.2 819.8 809.2 871.4 861.4 844.7 904.0 813.1 826.4 881.0

24 895.3 936.6 943.6 793.5 788.5 878.0 875.4 864.1 930.5 894.6 877.2 938.8 864.7 878.9 937.0

25 925.8 949.3 994.9 832.3 826.9 920.8 898.5 886.9 955.1 930.7 912.6 976.7 887.3 901.8 961.4

26 971.0 1036.5 1002.5 843.7 838.3 933.5 939.3 927.2 998.5 960.6 941.9 1008.0 948.5 964.1 1027.9

27 1042.1 1069.6 1118.8 885.5 879.8 979.7 972.1 959.5 1033.3 1056.1 1035.6 1108.3 1035.6 1052.6 1122.2

28 1103.2 1110.5 1206.3 935.5 929.5 1035.0 1049.3 1035.8 1115.4 1092.6 1071.4 1146.6 1074.4 1092.0 1164.2

29 1185.5 1296.3 1193.2 1001.8 995.4 1108.4 1088.7 1074.6 1157.2 1128.9 1107.0 1184.7 1135.5 1154.1 1230.4

30 1272.7 1359.5 1313.3 1048.5 1041.8 1160.1 1198.4 1182.8 1273.8 1214.5 1190.9 1274.5 1193.5 1213.1 1293.3

31 1366.1 1386.5 1482.4 1101.8 1094.7 1219.0 1286.9 1270.2 1367.9 1307.1 1281.7 1371.7 1291.9 1313.1 1399.9

32 1408.1 1500.0 1456.9 1167.9 1160.4 1292.2 1380.3 1362.5 1467.2 1397.9 1370.7 1466.9 1387.1 1409.8 1503.1

33 1483.9 1569.7 1546.4 1213.1 1205.3 1342.1 1426.4 1407.9 1516.2 1455.4 1427.2 1527.4 1458.2 1482.1 1580.1

34 1585.6 1698.5 1631.4 1264.5 1256.4 1399.1 1545.9 1525.9 1643.2 1584.3 1553.6 1662.6 1519.4 1544.3 1646.4

35 1722.6 1810.5 1806.9 1367.9 1359.1 1513.5 1626.4 1605.3 1728.8 1650.2 1618.1 1731.7 1558.1 1583.6 1688.3

99

Lampiran 16. Hasil Analisis Statistik Pengukuran Volume Air Minum


15 15 15 15 15

1353.2533 4294.8200 9208.5600 15874.53 25242.09

92.03141 355.01756 764.77375 1164.139 1875.378

.237 .214 .256 .186 .182

.113 .158 .165 .148 .145

-.237 -.214 -.256 -.186 -.182

.919 .828 .991 .722 .706

.368 .499 .280 .674 .701

N

Mean

Std. Deviation


Absolute

Positive

Negative

Most Extreme

Differences







1.915 4 10 .184

1.221 4 10 .362

.894 4 10 .503

.829 4 10 .536

.762 4 10 .573

minggu1

minggu2

minggu3

minggu4

minggu5

Levene


ANOVA

85445.071 4 21361.268 6.447 .008

33131.867 10 3313.187

118576.9 14

1430929 4 357732.141 10.724 .001

333595.9 10 33359.594

1764525 14

6687468 4 1671867.079 11.140 .001

1500836 10 150083.618

8188304 14

14526248 4 3631561.948 8.167 .003

4446811 10 444681.056

18973058 14

38104699 4 9526174.833 8.556 .003

11133874 10 1113387.399

49238573 14

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

minggu1

minggu2

minggu3

minggu4

minggu5

Sum of


100


minggu1

Duncana

3 1203.7333

3 1378.8333

3 1382.6667

3 1392.2000

3 1408.8333

1.000 .564

Perlakuan

P1

P3

P2

Po

P4

Sig.

N 1 2




minggu2

Duncana

3 3701.0667

3 4344.0000

3 4372.5000

3 4469.1667

3 4587.3667

1.000 .159

PerlakuanP1

P2

P4

P3

Po

Sig.

N 1 2




minggu3

Duncana

3 7891.4333

3 9397.5000

3 9492.7333

3 9526.4000

3 9734.7333

1.000 .343

PerlakuanP1

P2

P4

P3

Po

Sig.

N 1 2




101


minggu4

Duncana

3 13966.00

3 15999.77

3 16297.17

3 16355.40

3 16754.33

1.000 .225

Perlakuan

P1

P2

P4

P3

Po

Sig.

N 1 2




minggu5

Duncana

3 22180.27

3 25418.57

3 25909.07

3 25909.53

3 26793.00

1.000 .168

PerlakuanP1

P2

P4

P3

Po

Sig.

N 1 2




102

Lampiran 17. Data konversi pakan

Jenis

Perlakuan

Pengamatan (minggu)

1 2 3 4 5

P0

1 1.93 1.47 1.44 1.78 1.65

2 2.50 1.67 1.44 1.77 1.68

3 2.40 1.74 1.53 1.86 1.77

rata 2.28 1.63 1.47 1.80 1.70

sd 0.30 0.14 0.05 0.05 0.06

P1

1 2.07 1.33 1.15 1.32 1.29

2 1.81 1.33 1.13 1.30 1.30

3 2.38 1.49 1.29 1.44 1.50

rata 2.09 1.38 1.19 1.35 1.37

sd 0.29 0.09 0.08 0.08 0.12

P2

1 2.02 1.33 1.23 1.86 1.37

2 2.29 1.36 1.21 1.43 1.38

3 2.84 1.47 1.33 1.57 1.47

rata 2.38 1.39 1.25 1.62 1.41

sd 0.42 0.08 0.06 0.22 0.05

P3

1 1.74 1.41 1.18 1.39 1.45

2 1.77 1.30 1.28 1.37 1.39

3 1.84 1.42 1.34 1.42 1.48

rata 1.78 1.38 1.27 1.39 1.44

sd 0.05 0.07 0.08 0.03 0.05

P4

1 1.69 1.26 1.11 1.29 1.32

2 1.69 1.30 1.12 1.26 1.31

3 2.38 1.32 1.22 1.32 1.40

rata 1.92 1.29 1.15 1.29 1.34

sd 0.40 0.03 0.06 0.03 0.05

103

Lampiran 18. Analisis Statistik Konversi Pakan


15 15 15 15 15

2.0900 1.4133 1.2667 1.4920 1.4507

.35371 .13798 .12877 .21792 .14665

.160 .194 .145 .261 .168

.160 .194 .145 .261 .168

-.129 -.139 -.112 -.166 -.137

.620 .750 .563 1.011 .652

.836 .627 .909 .259 .788

N

Mean

Std. Deviation


Absolute

Positive

Negative

Most Extreme

Differences







2.012 4 10 .169

2.370 4 10 .122

.432 4 10 .783

4.654 4 10 .022

2.487 4 10 .111

minggu1

minggu2

minggu3

minggu4

minggu5

Levene


ANOVA

.732 4 .183 1.793 .207

1.020 10 .102

1.752 14

.189 4 .047 6.050 .010

.078 10 .008

.267 14

.183 4 .046 9.264 .002

.049 10 .005

.232 14

.549 4 .137 11.878 .001

.116 10 .012

.665 14

.250 4 .063 12.259 .001

.051 10 .005

.301 14

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

minggu1

minggu2

minggu3

minggu4

minggu5

Sum of


104


minggu1

Duncana

3 1.7833

3 1.9200

3 2.0867

3 2.2767

3 2.3833

.061

Perlakuan

P3

P4

P1

Po

P2

Sig.

N 1

Subset

for alpha

= .05



minggu2

Duncana

3 1.2933

3 1.3767

3 1.3833

3 1.3867

3 1.6267

.255 1.000

Perlakuan

P4

P3

P1

P2

Po

Sig.

N 1 2




minggu3

Duncana

3 1.1500

3 1.1900

3 1.2567

3 1.2667

3 1.4700

.087 1.000

PerlakuanP4

P1

P2

P3

Po

Sig.

N 1 2




105


minggu4

Duncana

3 1.2900

3 1.3533

3 1.3933

3 1.6200

3 1.8033

.287 .063

PerlakuanP4

P1

P3

P2

Po

Sig.

N 1 2




minggu5

Duncana

3 1.3433

3 1.3633

3 1.4067

3 1.4400

3 1.7000

.153 1.000

Perlakuan

P4

P1

P2

P3

Po

Sig.

N 1 2




106

Lampiran 19. Data Kadar Lemak kasar ,Protein Kasar dan Kolesterol total

Jenis Perlakuan Lemak Kasar

(%)

Protein Kasar

(%)

Kolesterol total

(mg/dl)

P0

1 25.17 18.59 169

2 25.4 18.34 167

3 25.2 18.54 168.75

rerata 25.26 18.49 168.25

sd 0.13 0.13 1.09

P1

1 11.71 21.76 154.5

2 11.83 21.77 153

3 11.3 21.29 154

rerata 11.61 21.61 153.83

sd 0.28 0.27 0.76

P2

1 10.5 22.73 152

2 10.63 22.61 151.76

3 10.41 22.5 153.2

rerata 10.51 22.61 152.32

sd 0.11 0.12 0.77

P3

1 11.71 23.27 151.15

2 11.59 23.29 150.75

3 11.53 23.34 150

rerata 11.61 23.30 150.63

sd 0.09 0.04 0.58

P4

1 11.74 23.9 149.83

2 11.27 23.86 149.1

3 11.52 23.85 148.27

rerata 11.51 23.87 149.07

sd 0.24 0.03 0.78

107

Lampiran 20. Analisis Statistik Kadar Lemak Kasar dan Protein Kasar


15 15 15

14.1007 21.9760 154.8207

5.79174 1.96979 7.17777

.452 .205 .318

.452 .164 .318

-.262 -.205 -.181

1.752 .794 1.231

.004 .555 .097

N

Mean

Std. Deviation


Absolute

Positive

Negative

Most Extreme

Differences



Lemak_kasar Protein_kasar

Kolesterol_

total




1.553 4 10 .260

5.802 4 10 .011

.570 4 10 .690

Lemak_kasar

Protein_kasar

Kolesterol_total

Levene


ANOVA

469.281 4 117.320 3475.128 .000

.338 10 .034

469.619 14

54.105 4 13.526 626.409 .000

.216 10 .022

54.321 14

714.652 4 178.663 269.387 .000

6.632 10 .663

721.284 14

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

Lemak_kasar

Protein_kasar

Kolesterol_total

Sum of


108


Lemak_kasar

Duncana

3 10.5133

3 11.5100

3 11.6100

3 11.6133

3 25.2567

1.000 .526 1.000

PerlakuanP2

P4

P3

P1

Po

Sig.

N 1 2 3




Protein_kasar

Duncana

3 18.4900

3 21.6067

3 22.6133

3 23.3000

3 23.8700

1.000 1.000 1.000 1.000 1.000

PerlakuanPo

P1

P2

P3

P4

Sig.

N 1 2 3 4 5




Kolesterol_total

Duncana

3 149.0667

3 150.6333

3 152.3200

3 153.8333

3 168.2500

1.000 1.000 1.000 1.000 1.000

Perlakuan

P4

P3

P2

P1

Po

Sig.

N 1 2 3 4 5




109

Lampiran 21. Simplisia segar

Temulawak

Daun Jinten Daun Pepaya

Sambiloto

110

Lampiran 22. Simplisia kering

Temulawak


Sambiloto

111

Lampiran 23. Serbuk simplisia

Daun Jinten

Sambiloto Temulawak

Daun Pepaya

112

Lampiran 24. Hasil Ekstraksi Dengan Rotary evaporator

`

Sambiloto


Temulawak

113

Lampiran 25. Hasil masing –masing ekstrak yang sudah dilarutkan

114

Lampiran 26. Kandang Ayam

115

Lampiran 27. Pakan, Vaksin dan desinfektan

Desinfektan dan vaksin

Pakan starter (umur 0-21 hari) Pakan finisher (umur 22- dijual)

116

Lampiran 28. Ayam dalam kadang dan Daging ayam semua perlakuan

117

Lampiran 29. Seperangkat alat penetapan kadar kolesterol total

\

Microlab 300 Reagen kit kolesterol

Reagen kit kolesterol

118

Lampiran 30. Alat Rotary evaporator dan Freez dryer

Rotary evaporator

Freez dryer

119

Lampiran 31. Seperangkat alat penetapan kadar lemak

.

Alat Soxhlet

120

Lampiran 32. Alat Penetapan Kadar Protein

108

Documents

Identifikasi ke LIPI Pembuatan Simplisia Maserasi dengan Etanol 80