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Introduction to the Compound Light Microscope. Chuck Hesbacker April 2010. Introduction to the Compound Light Microscope. Objectives:. Label the parts of the CLM Learn the function of the parts of the CLM Operate the CLM CLM = Compound Light Microscope. - PowerPoint PPT Presentation
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Introduction to the
Compound Light Microscope
Chuck HesbackerApril 2010
Introduction to the Compound Light Microscope
1. Label the parts of the CLM2. Learn the function of the parts of the
CLM3. Operate the CLM
CLM = Compound Light Microscope
Objectives:
Introduction to the Compound Light Microscope
Warm up exercise:Write down at least 5 things you know
about the microscope in your
class journals.
Introduction to the Compound Light Microscope
1. In 1590, Zaccharias Janssen and his father Hans invented the first microscope.
2. Galileo added a focusing device.
3. Anthony Leeuwenhoek was called the “Father of Microscopy” because he could obtain magnifications of 270X.
4. Leeuwenhoek was the first to see bacteria, yeast, and blood cells.
5. Robert Hooke was called the “English Father of the Microscope” because he made improvements to the microscope.
SOME USEFUL FACTS ABOUT WHO INVENTED THE MICROSCOPE
Introduction to the Compound Light Microscope
TODAY WE WILL1. Label the parts of the
CLM2. Learn the function of
the parts of the CLM3. Operate a CLM
CLM = Compound Light Microscope
Introduction to the Compound Light Microscope
Handout:Diagram of the
MicroscopeDon’t label the
diagram yet!
Introduction to the Compound Light Microscope
Fill out the blanks
as we proceed through
the lesson.
Introduction to the Compound Light Microscope
The body tube separates the objective and the eyepiece. Copy this into your lab manual.
Body Tube
Introduction to the Compound Light Microscope
The revolving nosepiece holds two or more objective lenses and can be rotated to change power.
Body Tube
Revolving Nosepiece
Introduction to the Compound Light Microscope
The 10x objective magnifies the image 10 times. It is the lowest power objective.
Body Tube
Revolving Nosepiece
10x Objective
Introduction to the Compound Light Microscope
The 43x objective magnifies the image 43 times. It is the medium power objective.
Body Tube
Revolving Nosepiece
10x Objective
43x Objective
Introduction to the Compound Light Microscope
The 97x objective magnifies the image 97 times. It is the high power objective and requires oil immersion.
Body Tube
Revolving Nosepiece
10x Objective
43x Objective
97x Objective
Introduction to the Compound Light Microscope
The stage clips hold the slides into position and prevent sliding.
Body Tube
Revolving Nosepiece
10x Objective
43x Objective
97x Objective
Stage Clips
Introduction to the Compound Light Microscope
The diaphragm controls the amount of light that passes through the object being viewed.
Body Tube
Revolving Nosepiece
10x Objective
43x Objective
97x Objective
Stage Clips
Diaphragm
Introduction to the Compound Light Microscope
The illuminator is a steady light source that illuminates the slide.
Body Tube
Revolving Nosepiece
10x Objective
43x Objective
97x Objective
Stage Clips
Diaphragm
Illuminator
Introduction to the Compound Light Microscope
The eyepiece lens is the lens at the top that you look through. They are usually 10X or 15X power.
Body Tube
Revolving Nosepiece
10x Objective
43x Objective
97x Objective
Stage Clips
Diaphragm
Illuminator
Eyepiece Lens
Introduction to the Compound Light Microscope
The arm supports the tube and connects it to the base.
Body Tube
Revolving Nosepiece
10x Objective
43x Objective
97x Objective
Stage Clips
Diaphragm
Illuminator
Eyepiece Lens
The Arm
Introduction to the Compound Light Microscope
The stage is the flat platform where you place your slides.
Body Tube
Revolving Nosepiece
10x Objective
43x Objective
97x Objective
Stage Clips
Diaphragm
Illuminator
Eyepiece Lens
The Arm
The Stage
Introduction to the Compound Light Microscope
The course focus is the larger knob that is first used to focus because it makes large adjustments to the focus.
Body Tube
Revolving Nosepiece
10x Objective
43x Objective
97x Objective
Stage Clips
Diaphragm
Illuminator
Eyepiece Lens
The Arm
The Stage
Coarse Focus
Introduction to the Compound Light Microscope
The fine focus is the smaller knob that makes small adjustments to the focus. Use the coarse focus first and then use the fine focus.
Body Tube
Revolving Nosepiece
10x Objective
43x Objective
97x Objective
Stage Clips
Diaphragm
Illuminator
Eyepiece Lens
The Arm
The Stage
Coarse Focus
Fine Focus
Introduction to the Compound Light Microscope
The base supports the microscope.
Body Tube
Revolving Nosepiece
10x Objective
43x Objective
97x Objective
Stage Clips
Diaphragm
Illuminator
Eyepiece Lens
The Arm
The Stage
Coarse Focus
Fine Focus
The Base
Introduction to the Compound Light Microscope
“USING THE COMPOUND MICROSCOPE”
Introduction to the Compound Light Microscope
Go to your laboratory station and setup your
microscope.
Setup slides on microscopes.
Examine the 8 slide set and
identify in book.
Body Tube
Revolving Nosepiece
10x Objective
43x Objective
97x Objective
Stage Clips
Diaphragm
Illuminator
Eyepiece Lens
The Arm
The Stage
Coarse Focus
Fine Focus
The Base
Introduction to the Compound Light Microscope
Return to your desk and label the parts of the microscope for a grade.
