Phenol Degrading Enzymes

8/13/2019 Phenol Degrading Enzymes

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1. Phenylphosphate synthase-Phenylphosphate synthase consists of three proteins whose genes are located

adjacent to each other on the phenol operon and were overproduced in Escherichia coli. The promoter region

and operon structure of the phenol gene cluster were investigated. Protein 1 (70 kDa) resembles the central part

of classical phosphoenolpyruvate synthase which contains a conserved histidine residue. It catalyzes the

exchange of free [(14)C]phenol and the phenol moiety of phenylphosphate but not the phosphorylation of

phenol. Phosphorylation of phenol requires protein 1, MgATP, and another protein, protein 2 (40 kDa), which

resembles the N-terminal part of phosphoenol pyruvate synthase. Proteins 1 and 2 catalyze the following

reaction: phenol + MgATP + H(2)O-->phenylphosphate + MgAMP + orthophosphate. The phosphoryl group in

phenylphosphate is derived from the beta-phosphate group of ATP. The free energy of ATP hydrolysis obviously

favors the trapping of phenol (K(m), 0.04 mM), even at a low ambient substrate concentration. The reaction is

stimulated severalfold by another protein, protein 3 (24 kDa), which contains two cystathionine-beta-synthase

domains of unknown function but does not show significant overall similarity to known proteins. The molecular

and catalytic features of phenylphosphate synthase resemble those of phosphoenolpyruvate synthase, albeit

with interesting modifications.

Pathway:

2.


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Protein-Blast of phenylphosphate synthase subunit gamma [Geobacter metallireducensGS-15] with genome of planococcus antarcticus.

Query-enzyme sequenceReference-Planococcus Antarcticus genome seq

P Blast- putative phenylphosphate synthase PpsB [Clostridia bacterium enrichment cultureclone BF]

P-Blast phenylphosphate synthase, beta subunit [Geobacter metallireducens GS-15]

P-Blast phenylphosphate synthase, alpha subunit [Geobacter metallireducens GS-15]


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2) Phenyl phosphate carboxylase- This enzyme consists of four proteins with molecular masses of 54, 53, 18, and

10 kDa, whose genes are located adjacent to each other in the phenol gene cluster which codes for phenol-

induced proteins. Three of the subunits (54, 53, and 10 kDa) were sufficient to catalyze the exchange of 14CO2

and the carboxyl group of 4-hydroxybenzoate but not phenylphosphate carboxylation. Phenylphosphate

carboxylation was restored when the 18-kDa subunit was added. The following reaction model is proposed. The

14CO2 exchange reaction catalyzed by the three subunits of the core enzyme requires the fully reversible release

of CO2 from 4-hydroxybenzoate with formation of a tightly enzyme-bound phenolate intermediate.

Carboxylation of phenylphosphate requires in addition the 18-kDa subunit, which is thought to form the same

enzyme-bound energized phenolate intermediate from phenylphosphate with virtually irreversible release of

phosphate. The 54- and 53-kDa subunits show similarity to UbiD of Escherichia coli, which catalyzes the

decarboxylation of a 4-hydroxybenzoate derivative in ubiquinone (ubi) biosynthesis. They also show similarity to

components of various decarboxylases acting on aromatic carboxylic acids, such as 4-hydroxybenzoate or

vanillate, whereas the 10-kDa subunit is unique. The 18-kDa subunit belongs to a hydratase/phosphatase protein

family. Phenylphosphate carboxylase is a member of a new family of carboxylases/decarboxylases that act on

phenolic compounds, use CO2 as a substrate, do not contain biotin or thiamine diphosphate, require K+ and a

divalent metal cation (Mg2+or Mn2+) for activity, and are strongly inhibited by oxygen.

P-Blast-phenylphosphate carboxylase, beta subunit [Geobacter metallireducens RCH3]

P-Blast-alpha subunit of phenylphosphate carboxylase [Aromatoleum aromaticum EbN1]

P-Blast-putative phenylphosphate carboxylase, gamma subunit [delta proteobacterium

NaphS2]


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P-Blast-phenylphosphate carboxylase subunit delta [Aromatoleum aromaticum]

Xylanase-Xylanases are hydrolytic enzymes which randomly cleave the beta 1,4 backbone of the complex plant

cell wall polysaccharide xylan. Diverse forms of these enzymes exist, displaying varying folds, mechanisms of

action, substrate specificities, hydrolytic activities (yields, rates and products) and physicochemical

characteristics. Research has mainly focused on only two of the xylanase containing glycoside hydrolase families,

namely families 10 and 11, yet enzymes with xylanase activity belonging to families 5, 7, 8 and 43 have also been

identified and studied, albeit to a lesser extent. Driven by industrial demands for enzymes that can operate under

process conditions, a number of extremophilic xylanases have been isolated, in particular those from

thermophiles, alkaliphiles and acidiphiles, while little attention has been paid to cold-adapted xylanases. Here,

the diverse physicochemical and functional characteristics, as well as the folds and mechanisms of action of all six

xylanase containing families will be discussed. The adaptation strategies of the extremophilic xylanases isolatedto date and the potential industrial applications of these enzymes will also be presented.

P-Blast Xylanase[Filobasidium floriforme]

Laccase-

P-Blast laccase [Pinus mugo]


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Catechol 1,2-dioxygenase-

P-Blast- catechol 1,2-dioxygenase [Acinetobacter baumannii]

Lignin peroxidase-

P-Blast-lignin peroxidase [Trametes versicolor]

Manganese peroxidase-

P-Blast-manganese peroxidase [Trametes versicolor]


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MetaCycPathway: phenol degradation I (aerobic):

Summary:

Phenols and methyl-substituted phenols can be used by a number of different bacterial and fungal strains

including Pseudomonas sp. CF600, which grows efficiently with phenol, cresols, or 3,4-dimethylphenol as the solecarbon and energy source.

The most common first step in the aerobic degradation of phenols is hydroxylation to a catechol. In

Pseudomonas sp. CF600 this reaction is catalyzed by a multicomponent phenol 2-monooxygenase, encoded by

six different gene that are located on pVI150, an IncP-2 megaplasmid [Shingler92, Powlowski94].

These genes are clustered along with 9 other genes that are involved in the catabolism of the catechol to TCA

cycle intermediates via a meta-cleavage pathway (see catechol degradation II (meta-cleavage pathway)).

Similar operons were found not only on plasmids but also in the chromosomes of some phenol-degrading

bacteria such as Pseudomonas putida P35X [Ng94] and Acinetobacter calcoaceticus [Xu03], as well as fungi such

as Trichosporon cutaneum [Gerginova].

Phenol 2-monooxygenase:

P-Blast: phenol 2-monooxygenase [Sulfobacillus acidophilus DSM 10332]
http://metacyc.org/META/organism-summary?object=METAhttp://metacyc.org/META/organism-summary?object=METAhttp://metacyc.org/META/organism-summary?object=META


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P-Blast Polyphenol oxidase:

Conserved hypothetical protein E-Coli (243 AA):Laccase domain protein

yfiH [Escherichia coli PMV-1]NCBI Reference Sequence: YP_008572618.1

hypothetical protein A1A1_18242

[Planococcus antarcticus DSM 14505]GenBank: EIM05031.1


8/18

laccase [Bacillus subtilis]NCBI Reference Sequence: WP_019714343.1


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gi|518544136|ref|WP_019714343.MNTYHPFSLTTPSTLMIQDWIQTNQNNREVIAGFTTKNGGVSQKPFESLN 50gi|388462627|gb|EIM05031.1| ------------------MKAKLYVNDGQYVSGMTLKDLAEP----ESNN 28

: *: : ::*:* *:. . ** *

gi|518544136|ref|WP_019714343.TGLHVHDKDADVVKNREYIADMFNIDLQSWVFADQTHDNRVQKVTQRDRG 100gi|388462627|gb|EIM05031.1|MALHACENPQSVLANRKKLADFISAPVSSFICAQQTHSANFRRVSAKDKG 78

.**. :: .*: **: :**::. :.*:: *:***...::*: :*:*

gi|518544136|ref|WP_019714343.KGAREYHTALKATDGLYTNEKNVFLALCFADCVPLFFYDPVKSLIGVAHA 150gi|388462627|gb|EIM05031.1|RGAYDTEDAFSDTDALYTYDSRVVLCSFAADCVPVIIHDRKTGLIGVVHS 128

:** : . *:. **.*** :..*.*. *****::::*..****.*:

gi|518544136|ref|WP_019714343.GWKGTVKQIGREMVKQWTEQEGSNPSDIYAVIGPSISGACYTVDDRVMDA 200gi|388462627|gb|EIM05031.1|GWQGTIKEIMSKLLQQLIDLEQCDPVDLDIQIGAAISQQQFEVDQDVYSK 178

**:**:*:* ::::* : * .:* *: **.:**: **: * .

gi|518544136|ref|WP_019714343.VRALPVSAERAVNQAAQAQYQLDLKELNRLILIDSGLVNEQISVSGLCTE 250gi|388462627|gb|EIM05031.1|LDSLGYASEFMYFNNMTKKYHIDNQATVKRQCELVGVTSGQIHIDPTCTF 228

: :* ::* : :*::* : : *:..

** :. **

gi|518544136|ref|WP_019714343. SEPSLFYSHRRDQGKTGRMMSFIGMKEA 278


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gi|388462627|gb|EIM05031.1| LSLDGFS--YRQDRKSGRHLIFAMKK-- 252. . * *:: *:** : * *

VSGMTLKDLAEPESNNMALHACENPQSVLANRKKLADFISAPVSSFICAQQTHSANFRRV

SAKDKGRGAYDTEDAFSDTDALYTYDSRVVLCSFAADCVPVIIHDRKTGLIGVVHSGWQG

TIKEIMSKLLQQLIDLEQCDPVDLDIQIGAAISQQQFEVDQDVYSKLDSLGYASEFMYFN

NMTKKYHIDNQATVKRQCELVGVTSGQIHIDPTCTFLSLDGFSYRQDRKSGRHLIF


11/18

Pepstats analysis of above sequence:(for amino acid percentage content)PEPSTATS of EMBOSS_001 from 1 to 236

Molecular weight = 26315.70 Residues = 236Average Residue Weight = 111.507 Charge = -3.5Isoelectric Point = 5.5698A280 Molar Extinction Coefficients = 17420 (reduced) 17795 (cystine bridges)A280 Extinction Coefficients 1mg/ml = 0.662 (reduced) 0.676 (cystine bridges)Probability of expression in inclusion bodies = 0.582

Residue Number Mole% DayhoffStat

A = Ala 18 7.627 0.887B = Asx 0 0.000 0.000C = Cys 7 2.966 1.023D = Asp 21 8.898 1.618E = Glu 9 3.814 0.636F = Phe 11 4.661 1.295G = Gly 13 5.508 0.656H = His 7 2.966 1.483I = Ile 15 6.356 1.412J = --- 0 0.000 0.000K = Lys 13 5.508 0.835L = Leu 19 8.051 1.088M = Met 5 2.119 1.246N = Asn 8 3.390 0.788

O = --- 0 0.000 0.000P = Pro 6 2.542 0.489Q = Gln 16 6.780 1.738R = Arg 10 4.237 0.865S = Ser 21 8.898 1.271T = Thr 12 5.085 0.834U = --- 0 0.000 0.000V = Val 16 6.780 1.027W = Trp 1 0.424 0.326X = Xaa 0 0.000 0.000Y = Tyr 8 3.390 0.997Z = Glx 0 0.000 0.000

Property Residues Number Mole%Tiny (A+C+G+S+T) 71 30.085

Small (A+B+C+D+G+N+P+S+T+V) 122 51.695Aliphatic (A+I+L+V) 68 28.814


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Aromatic (F+H+W+Y) 27 11.441Non-polar (A+C+F+G+I+L+M+P+V+W+Y) 119 50.424Polar (D+E+H+K+N+Q+R+S+T+Z) 117 49.576Charged (B+D+E+H+K+R+Z) 60 25.424Basic (H+K+R) 30 12.712Acidic (B+D+E+Z) 30 12.712

ProtParam Analysis of above sequence for unstability index:Number of amino acids:236

Molecular weight:26315.7

Theoretical pI:5.45

Amino acid composition:CSV format

Ala (A) 18 7.6%Arg (R) 10 4.2%Asn (N) 8 3.4%Asp (D) 21 8.9%

Cys (C) 7 3.0%Gln (Q) 16 6.8%Glu (E) 9 3.8%Gly (G) 13 5.5%His (H) 7 3.0%Ile (I) 15 6.4%Leu (L) 19 8.1%Lys (K) 13 5.5%Met (M) 5 2.1%Phe (F) 11 4.7%Pro (P) 6 2.5%Ser (S) 21 8.9%Thr (T) 12 5.1%

Trp (W) 1 0.4%Tyr (Y) 8 3.4%Val (V) 16 6.8%Pyl (O) 0 0.0%Sec (U) 0 0.0%

(B) 0 0.0%(Z) 0 0.0%(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu):30Total number of positively charged residues (Arg + Lys):23

Atomic composition:

Carbon C 1154Hydrogen H 1810Nitrogen N 318Oxygen O 362Sulfur S 12

Formula:C1154H1810N318O362S12Total number of atoms:3656

Extinction coefficients:

Extinction coefficients are in units of M-1cm-1, at 280 nm measured inwater.


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Ext. coefficient 17795Abs 0.1% (=1 g/l) 0.676, assuming all pairs of Cys residues form cystines

Ext. coefficient 17420

Abs 0.1% (=1 g/l) 0.662, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is V (Val).

The estimated half-life is: 100 hours (mammalian reticulocytes, in vitro).>20 hours (yeast, in vivo).>10 hours (Escherichia coli, in vivo).

Instability index:

The instability index (II) is computed to be 42.84This classifies the protein as unstable.

Aliphatic index:83.47

Grand average of hydropathicity (GRAVY):-0.260

Secondary structure elements prediction(NSP@):

Multivariate Linear Regression Combination (SOPMA-

GOR4-SIMPA) result for : UNK_154910

AbstractGuermeur et al. submitted

View MLRC in: [AnTheProt (PC),Download...][HELP]

10 20 30 40 50 60 70| | | | | | |

VSGMTLKDLAEPESNNMALHACENPQSVLANRKKLADFISAPVSSFICAQQTHSANFRRVSAKDKGRGAYcccceccccccccccheeeeccccchhhhhhhhhhhhhhccccchhhehhhcccceeeeeecccccccccDTEDAFSDTDALYTYDSRVVLCSFAADCVPVIIHDRKTGLIGVVHSGWQGTIKEIMSKLLQQLIDLEQCD

cccccccccceeeecccceeeeeeccccceeeeeccccceeeeeeccchhhhhhhhhhhhhhhhhhccccPVDLDIQIGAAISQQQFEVDQDVYSKLDSLGYASEFMYFNNMTKKYHIDNQATVKRQCELVGVTSGQIHIccceeeeecccccccccccchhhhhhhhhhccccceeecccccccceecchhhhhhhhhhccccccceeeDPTCTFLSLDGFSYRQDRKSGRHLIFcccceeccccceeccccccccceeccSequence length : 236MLRC :

Alpha helix (Hh) : 59 is 25.00%310 helix (Gg) : 0 is 0.00%Pi helix (Ii) : 0 is 0.00%Beta bridge (Bb) : 0 is 0.00%Extended strand (Ee) : 52 is 22.03%Beta turn (Tt) : 0 is 0.00%

Bend region (Ss) : 0 is 0.00%Random coil (Cc) : 125 is 52.97%
http://npsa-pbil.ibcp.fr/NPSA/npsa_references.html#MLRhttp://npsa-pbil.ibcp.fr/NPSA/npsa_references.html#MLRhttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.mlr.mpsa&gui=antheprothttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.mlr.mpsa&gui=antheprothttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.mlr.mpsa&gui=antheprothttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.mlr.mpsa&gui=antheprothttp://antheprot-pbil.ibcp.fr/http://antheprot-pbil.ibcp.fr/http://antheprot-pbil.ibcp.fr/http://npsa-pbil.ibcp.fr/cgi-bin/npsa_automat.pl?page=/NPSAHLP/npsahlp_locsoft.htmlhttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_automat.pl?page=/NPSAHLP/npsahlp_locsoft.htmlhttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_automat.pl?page=/NPSAHLP/npsahlp_locsoft.htmlhttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_automat.pl?page=/NPSAHLP/npsahlp_locsoft.htmlhttp://antheprot-pbil.ibcp.fr/http://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.mlr.mpsa&gui=antheprothttp://npsa-pbil.ibcp.fr/NPSA/npsa_references.html#MLR


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Ambigous states (?) : 0 is 0.00%Other states : 0 is 0.00%

Prediction result file (text): [MLRC]Intermediate result files (text) : [GOR4][SIMPA96][SOPMA]

View intermediate result files in MPSA : [GOR4][SIMPA96][SOPMA]View intermediate result files in ANTHEPROT : [GOR4][SIMPA96][SOPMA]

Prediction of Secondary Structural Content from Amino Acid Composition

# with Analytic Vector Decomposition

# ----------------------------------------------------------------------

#

Input-Type: Sequence

VSGMTLKDLAEPESNNMALHACENPQSVLANRKKLADFISAPVSSFICAQQTHSANFRRV

SAKDKGRGAYDTEDAFSDTDALYTYDSRVVLCSFAADCVPVIIHDRKTGLIGVVHSGWQG

TIKEIMSKLLQQLIDLEQCDPVDLDIQIGAAISQQQFEVDQDVYSKLDSLGYASEFMYFNNMTKKYHIDNQATVKRQCELVGVTSGQIHIDPTCTFLSLDGFSYRQDRKSGRHLIF

#

# Thank you for using the WWW-server for SSCP !

#

#

#

# 1st METHOD

# The first method relies only on the average amino acid composition

# of secondary structural segments (helix, sheet, coil) in a learning

# set of proteins.

#

method : 1
http://npsa-pbil.ibcp.fr/tmp/b7737141cbc5.mlrhttp://npsa-pbil.ibcp.fr/tmp/b7737141cbc5.mlrhttp://npsa-pbil.ibcp.fr/tmp/b7737141cbc5.gor4http://npsa-pbil.ibcp.fr/tmp/b7737141cbc5.gor4http://npsa-pbil.ibcp.fr/tmp/b7737141cbc5.gor4http://npsa-pbil.ibcp.fr/tmp/b7737141cbc5.simpa96http://npsa-pbil.ibcp.fr/tmp/b7737141cbc5.simpa96http://npsa-pbil.ibcp.fr/tmp/b7737141cbc5.simpa96http://npsa-pbil.ibcp.fr/tmp/b7737141cbc5.sopmahttp://npsa-pbil.ibcp.fr/tmp/b7737141cbc5.sopmahttp://npsa-pbil.ibcp.fr/tmp/b7737141cbc5.sopmahttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.gor4.mpsa&gui=mpsahttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.gor4.mpsa&gui=mpsahttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.gor4.mpsa&gui=mpsahttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.simpa96&gui=mpsahttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.simpa96&gui=mpsahttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.simpa96&gui=mpsahttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.sopma&gui=mpsahttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.sopma&gui=mpsahttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.sopma&gui=mpsahttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.gor4.mpsa&gui=antheprothttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.gor4.mpsa&gui=antheprothttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.gor4.mpsa&gui=antheprothttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.simpa96&gui=antheprothttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.simpa96&gui=antheprothttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.simpa96&gui=antheprothttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.sopma&gui=antheprothttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.sopma&gui=antheprothttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.sopma&gui=antheprothttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.sopma&gui=antheprothttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.simpa96&gui=antheprothttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.gor4.mpsa&gui=antheprothttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.sopma&gui=mpsahttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.simpa96&gui=mpsahttp://npsa-pbil.ibcp.fr/cgi-bin/npsa_mpsaview.pl?fn=/tmp/b7737141cbc5.gor4.mpsa&gui=mpsahttp://npsa-pbil.ibcp.fr/tmp/b7737141cbc5.sopmahttp://npsa-pbil.ibcp.fr/tmp/b7737141cbc5.simpa96http://npsa-pbil.ibcp.fr/tmp/b7737141cbc5.gor4http://npsa-pbil.ibcp.fr/tmp/b7737141cbc5.mlr


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alpha-contents : 33.3 %

beta-contents : 26.2 %

coil-contents : 40.5 %

class : mixed

#

##

# 2nd METHOD

# The second method relies also on composition fluctuations in the

# secondary structural segments (helix, sheet, coil) of a learning

# set of proteins.

#

method : 2

alpha-contents : 54.3 %

beta-contents : 7.2 %

coil-contents : 38.5 %

class : alpha

#

#

CLUSTAL W Alignment

Multiple Alignment of Phenylphosphate synthase alpha subunit:

Sequences from:-

phenylphosphatesynthasesubunit alpha[Desulfobacula toluolica Tol2]

putative phenylphosphatesynthase, alphasubunit [Azoarcus sp. KH32C]

phenylphosphatesynthase, alphasubunit [Geobacter metallireducens GS-15]

putative phenylphosphate synthase alpha subunit PpsA [Clostridia bacterium enrichment culture clone BF]

Phenylphosphate synthasealphasubunit [Aromatoleum aromaticum EbN1]

Editing in Box shade:-gi|470203970|ref|YP_007598069. 1YDGLHYPEPLYPFDTIWDEAWYLALSQFNNRIFQVPPVRGVDHRIINGYV 97YISPVPVgi|56313243|emb|CAI07888.1| 1YDGLHYPEPLYPFDTIWDEAWYLALSQFNNRIFQVPPVRGVDHRIINGYV 97YISPVPVgi|78194562|gb|ABB32329.1| 1

YDGLHYPEPIYPFDTIWDEAWYLGLSQYNNRIFQVPPVRGVDHRIINGYV95YISPVPVgi|408419048|ref|YP_006760462. 1NDALHYPEPLYPFDIIWDEAWFLALSQFNTRIFSVPPVYGVDHRIINGNV98YISPVPVgi|300245771|gb|ADJ93943.1| 1 HDSVHFNPPCTPMGASLNINCMRG-SHWAAEWFSLPFSLGFETQVYNGYV77YHAVNPI

gi|470203970|ref|YP_007598069. 58KDPDEIGRRVPNFMERAGYYYKNWDELEAKWKTKMEGTIAELE147ALQIPRLPEVEDLgi|56313243|emb|CAI07888.1| 58KDPAEIGARVPNFMERAGFYYKNWDALEAKWKVKMEAAIAELE147ALEIPPLPDVEALgi|78194562|gb|ABB32329.1| 58TDPEEVGSRVPNFMERAGYYYKNWNELEAKWELKMKGIIKQIE145ELPIPSLPDLEDI

gi|408419048|ref|YP_006760462. 58TDPEEVQKRIPMFMERAGYYYENWDDLHNQWENKMKGIISEIE148NLEIASLPDMEDIgi|300245771|gb|ADJ93943.1| 57
http://www.ncbi.nlm.nih.gov/protein/YP_006760462.1http://www.ncbi.nlm.nih.gov/protein/YP_006760462.1http://www.ncbi.nlm.nih.gov/protein/YP_006760462.1http://www.ncbi.nlm.nih.gov/protein/YP_006760462.1http://www.ncbi.nlm.nih.gov/protein/YP_006760462.1http://www.ncbi.nlm.nih.gov/protein/YP_006760462.1http://www.ncbi.nlm.nih.gov/protein/YP_006760462.1http://www.ncbi.nlm.nih.gov/protein/YP_007598069.1http://www.ncbi.nlm.nih.gov/protein/YP_007598069.1http://www.ncbi.nlm.nih.gov/protein/YP_007598069.1http://www.ncbi.nlm.nih.gov/protein/YP_007598069.1http://www.ncbi.nlm.nih.gov/protein/YP_007598069.1http://www.ncbi.nlm.nih.gov/protein/YP_007598069.1http://www.ncbi.nlm.nih.gov/protein/YP_007598069.1http://www.ncbi.nlm.nih.gov/protein/YP_007598069.1http://www.ncbi.nlm.nih.gov/protein/ABB32329.1http://www.ncbi.nlm.nih.gov/protein/ABB32329.1http://www.ncbi.nlm.nih.gov/protein/ABB32329.1http://www.ncbi.nlm.nih.gov/protein/ABB32329.1http://www.ncbi.nlm.nih.gov/protein/ABB32329.1http://www.ncbi.nlm.nih.gov/protein/ABB32329.1http://www.ncbi.nlm.nih.gov/protein/ABB32329.1http://www.ncbi.nlm.nih.gov/protein/CAI07888.1http://www.ncbi.nlm.nih.gov/protein/CAI07888.1http://www.ncbi.nlm.nih.gov/protein/CAI07888.1http://www.ncbi.nlm.nih.gov/protein/CAI07888.1http://www.ncbi.nlm.nih.gov/protein/CAI07888.1http://www.ncbi.nlm.nih.gov/protein/CAI07888.1http://www.ncbi.nlm.nih.gov/protein/CAI07888.1http://www.ncbi.nlm.nih.gov/protein/ABB32329.1http://www.ncbi.nlm.nih.gov/protein/YP_007598069.1http://www.ncbi.nlm.nih.gov/protein/YP_006760462.1


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TDPAKIEARAVEFGPKLQKALDNWNDFYGEGVQEWSDLLNYLA 127GFQKETLP-----

gi|470203970|ref|YP_007598069. 114RVVTDGVGESTAYHLLKNYDDLINLGIRCWQYHFEFL197NLGYAAYVFFMDFTQKLFPgi|56313243|emb|CAI07888.1| 114

SVVTDGVGESKGYHLLKNYDDLINLGIKCWQYHFEFL197NLGYAAYVFFMDFTQKLFPgi|78194562|gb|ABB32329.1| 114SVVTEGIGESKGFHLIKAYDDLINLGIQCWQYHFEFL195NLGYAAYVFFLDFVQKLFPgi|408419048|ref|YP_006760462. 114SVVKNGLGTSSGYELLKNYDKLIDLGIRCWQYHFEFL198NLGYASYVTFVDFCTKAFPgi|300245771|gb|ADJ93943.1| 108 --------FDRLHELLKDAEKTFKR---SWELHFIYM161YPSFFGYMNFEAICQKYN-

gi|470203970|ref|YP_007598069. 170 SIPLQRVTQMISGIDVIMYKSDDELKELAKK247AVALEVD-DIVTAHREWADVKAAMAgi|56313243|emb|CAI07888.1| 170 SIPMQRVTQMISGIDVIMYRPDDELKELAKK247AVKLEVD-DIVAGHREWADVKAALA

gi|78194562|gb|ABB32329.1| 170 SIPLQRVTQMIAGIDVIMYRSDDELKKLAKK245AIELGVD-LILTNCGEWQDAETALKgi|408419048|ref|YP_006760462. 170 DIPLQKVTQMVAGIDVILYRPDDELRKLAKL248AIELGIDSQIGGDTKDIDDVISELQgi|300245771|gb|ADJ93943.1| 152 -INERDMRTFLQGFETKMFEIDREMWHLADL209AREMGLQ-TVFDVAQKSEDIKGLMK

gi|470203970|ref|YP_007598069. 225 GRRHGDEWLAAFEKARYPWFNISSG296TGWFHTDRSWNDNLNLPLDGIQTYIGKLRDGgi|56313243|emb|CAI07888.1| 225 GHRHGIEWLAAFEKARYPWFNISSG296TGWFHTDRSWNDNLDIPLDGIQTYIGKLRAGgi|78194562|gb|ABB32329.1| 225 AHPKGRTWLDALEEARYPWFHVSTG294TGWSHSDKSWNDDLNIPLSGIATYINQLRQGgi|408419048|ref|YP_006760462. 226 GSENGKKWVAAWDEAKYPWFNISTG298TGWYHQDVSWNDNLNIPLSSVRIYIGKLNEGgi|300245771|gb|ADJ93943.1| 206 EKKLGVVWWDQFERFLSKYGKRSTA258ALFDMYYKTWNEDPYPVLFTIKTYIQKG--G

gi|470203970|ref|YP_007598069. 281 VAIDRPMEAVRAERDRVTA346EYRDLIDNDQDRKQFDELLGCARTVFPYVENHLFYVEgi|56313243|emb|CAI07888.1| 281 VSIDRPMEAVRAERERITA346EYRELIDSDEDRKQFDELLSCAKTVFPYVENHLFYVEgi|78194562|gb|ABB32329.1| 281 MDIERPMEKVRAERDRITE344EYRDLITSDEDRKTFDELLGTAKTVFPYVENHLFYVEgi|408419048|ref|YP_006760462. 282 KSIDRPLEQIKIERKKLIV348EYRGLLKTDEDRQTFDQLHGTAELVFPYVENHMFYVEgi|300245771|gb|ADJ93943.1| 260 FDFEEHTKKITDEREKLIE 306ETIARIPAE-NREEFRIALKHAQDSYPFNEDHNFYVE

gi|470203970|ref|YP_007598069. 337 HWFHSVFWNKMRE396VAAIMQEHGVIAEIEDIWLLRRDEIKQALWDIVTAWATGVTPRgi|56313243|emb|CAI07888.1| 337 HWFHSVFWNKMRE396VAAIMKEHRMIADIEDIWLLRRDEIKQALWDVVTAWATGVTPRgi|78194562|gb|ABB32329.1| 337 HWFHTVFWRKMRE394VAAIMKEHGMFEDVEDVWYLRRDEIKQGLWDMVTAWATGVKPRgi|408419048|ref|YP_006760462. 338 HWFHSVFWNKMRA398VAAIMADNNFLEGQEDIWYMSRSEIKEALWDLVTAWASGTKGY

gi|300245771|gb|ADJ93943.1| 315 HWTHCEIRYVILE355CGRRLVEMGILKEADDVFFLTIGELKGFMEEIIMDERVGIQYF


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gi|470203970|ref|YP_007598069. 393 GTKTWPA446EIEWRKGVMQKFREWAPPPAIGIAPE-VIQEPFTIVLWGVTNSSLSAWAgi|56313243|emb|CAI07888.1| 393 GTATWPA446EIEWRKGVMQKFREWNPPPAIGVAPE-VIQEPFTIVLWGVTNNSLSDWA

gi|78194562|gb|ABB32329.1| 393 GTAVWPK444EIAWRKGVMKKFQEWSPPPAVGIPPE-VIREPFTIVLWGVTNSSISDWSgi|408419048|ref|YP_006760462. 394 GPLHWPE448EIQWRKGVYQKFKENIPIPAVGKPPE-TIAEPFTIVLWGITNESMSAWAgi|300245771|gb|ADJ93943.1| 371 GTKVTNT 405VYERKQ-VWQDMHEFDAPPFIGTIPEHKIEDPVFIKVFGMTDEVIRGSA

gi|470203970|ref|YP_007598069. 448 S495VQEVADPDSITELKGFPASPGTVEGKARVCRSAEEIRDLQEGEILVAPTT545Sgi|56313243|emb|CAI07888.1| 448 A495VQDIGDPDSITELKGFAASPGTVEGRARVCRSAEDIRDLQEGEILVAPTT545Sgi|78194562|gb|ABB32329.1| 448 E

493VQDISDPDSITTLKGFAGSPGVVEGKARVCRSADDIRLLQEGEILVAPTT543Sgi|408419048|ref|YP_006760462. 449 K497LKEIGDPDKVNEMEGFAGSPGVAEGIARVCRSVEDIGELKEGEILVAPTT547Sgi|300245771|gb|ADJ93943.1| 426 R 454NTEHVAG----HFEGFPGAPGTVEGVARVVFNYEDFSTVQPDELLVAPFT500T

gi|470203970|ref|YP_007598069. 500PSWAPAFAKIKACITDVGGVMSHAAIVCREYGMPAVVGTGHS----TRV591IKTGMTLgi|56313243|emb|CAI07888.1| 500PSWAPAFAKIKACVTDVGGVMSHAAIVCREYGMPAVVGTGVS----TRV591IRTGMTLgi|78194562|gb|ABB32329.1| 500PSWAPAFARIKGAITDVGGVMSHAAIVCREYGMPAVVGTGHA----TKI589IRTGMMLgi|408419048|ref|YP_006760462. 501PSWAPVFQKIKAVVTDVGGIMCHAAIVCREYGMPAVVGTGQG----TTI593IKSGMKIgi|300245771|gb|ADJ93943.1| 474PAWTPLFSKIRGVVTDSGGMLAHAAICAREYDIPAVVGTITRGVKVTEH 550IKTGDRI


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gi|470203970|ref|YP_007598069. 552 RVDGSSGLVTIVQ- 611gi|56313243|emb|CAI07888.1| 552 RVDGSSGVISIITD 612gi|78194562|gb|ABB32329.1| 552 RVDGATGVVTIDR- 609gi|408419048|ref|YP_006760462. 553 KVNGDTGKIHIER- 613gi|300245771|gb|ADJ93943.1| 530 RIDGTNGKVEIIV- 570

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Phenol Degrading Enzymes