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ISSUE 12 - NOVEMBER 2008 • ValId UNtIl 31St MaRch 2009 • chEcK OUt WWW.VWR.cOM FOR FUll SPEcIFIcatIONS
http://de.vwr.com
Local price agreements apply.
Shop!ISSUE 12 NOVEMbEr 2008in this issue
DE
www.vwr.comeditorVWr International Europe bvbaHaasrode researchpark Zone 3Geldenaaksebaan 4643001 Leuvenbelgium
copywritingVWr International
Layout and typesettingMarketing Services VWr
PrintingStork, Germany
No part of this publication may be reproduced or copied without prior permission by writing of VWr International Europe.
run80.500 copiesPublication date: November 2008
Due to the high sales volume of promoted articles someitems may be temporarily out of stock - VWr Terms andConditions of Sale apply.
capitalBio Luxscan™ 10K – A dual channel confocal microarray scanner - that you cAn afford!
A compact high performance scanner system for microarray imaging and data analysis of DNA, protein and cell arrays. Integrating many advanced optical, signal processing and motion control systems, the LuxScan™
10K has two excitation lasers and provides high detection sensitivity, accuracy, dynamic range and linearity. Advanced and optimised signal acquisition and processing elements, make it an ideal tool for profiling microarray-based gene expression assays.
Laser intensity and PMT are continuously adjustable•Superior image quality, Signal to Noise ratio •exceeds other equivalent productsFree software upgrades for first 5 years•
To claim simply email the serial number of the unit to [email protected] with your choice
up for DoPPio Pcr?The VWr Collection DOPPIO thermal cycler is an ideal way to maximise research funds by investing in a really flexible cycler. Two independent 48 well blocks, both with sprung heated lids will adapt to both tubes and high profile 48 well plates. Two programs can be run independently or one program simultaneously on both blocks. Either way, the DOPPIO provides two-block functionality at an incredible price.
Want gradients? Who wouldn’t?! The DOPPIO can run gradient programs on both or either thermal blocks as a standard feature. Simply enter a gradient step into your protocol, and the DOPPIO will generate a gradient over 6 columns. For a wider gradient range, then simply use both blocks.
As part of the VWr Collection thermal cycler family, the DOPPIO benefits from the same software architecture as the other products in the range, using Windows CE as its control platform, with a 3,5” full colour touch screen. Users are able to store up to 1000 programs within the onboard software.
molecular Biologypromo
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special intro price and 200 euro of free oligos
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molecular BiologyNew DOPPIO thermal cycler from the •VWr Collection
Capitalbio LuxScan• ™ microarray scannerNew KOD DNA Polymerases from •Merck’s Novagen® rangeOmega biotek E.Z.N.A large scale plasmid •preparation kitsThermo Scientific barcoded 96 well PCr •platesPCr Mycoplasma Test Kit•Three clicks away from pure DNA with •Mole!Easy to use custom oligonucleotide •online order tool!
electrophoresisIt’s time for 5 PrIME’s perfect PCr •partnersPrecast gels for protein electrophoresis •from LonzaCbS Scientific adjustable slab gel kit •
cell biologyAutovaxID• ™ cell culture instrument increases mAb concentration by 50%Efficient Fusion with bTX•brANDplates - discover the broad •range of the new microplates for immunological applications!Ultimate low cell binding cultureware •from NuncbD Primaria• ™ cell culture products NALGENE• ® high speed centrifuge tubes and bottlesWheaton 2 ml cryogenic sterile Cryule• ® vialsVWr Collection incubating orbital •shaker
sample prepSimple, reliable concentrating and •desalting with Pall NanoSep devicesready-to-use dialysis device from •Spectrum
microplate readingEnhance your kinetic assays with •the Thermo Scientific microplate fluorometers and luminometers! Chip Format (mm) 25 × 75
ScanResolution(μm) User selectable: 5, 10, 20, 40
Sensitivity 0,1fluorescentmolecules/μm2
Scan Speed 8mins/chip(10μmresolution)
Dynamic range 104
repeatability 95%
Laser Power Settings 1-100% continuously adjustable
PMT Gain 450-950 continuously adjustable
Maximum Scan Area (mm) 22 × 72
FocusSearchRange(μm) ± 250
Excitation Wavelength (nm) 532 and / or 635
Applicable Fluorescence Dyes Cy3, Cy5, etc.
Emission Wavelength 570 ± 10 nm, 675 ± 10 nm (expandable to 6 types)
Image File Format TIFF, bMP, JPG, rAW
Size (W × D × H) (mm) 360 × 540 × 220
Weight (kg) 40
e.Z.n.A.® large scale plasmid preparation kits
Takes less than 40 minutes•High quality plasmid suitable •for most downstream applicationsLow endotoxin levels for •Plasmid Mega and Giga PrepsVacuum protocol saves 20 •minutes over centrifugation protocol for Midi and Maxi Preps
*Limit one per customer ** Includes free vacuum manifold (Vac-08P)
order a large scale plasmid kit and get a vacuum
manifold free*
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Great price and over 700€ of free accessories!
new KoD DnA Polymerases from merck’s novagen® rangeKoD Xtreme™ hot start DnA Polymerase
Amplification of difficult DNA targets•Amplifies DNA with up to 90% GC-content •Amplifies targets up to 40 kbp •Optimised for the highest success rate•
KoD hot start master mix - our top performing Pcr Polymerase now as master mix!
Highest accuracy, yield and processivity •Amplifies templates up to 21 kbp •Eliminates mispriming and primer-dimer formation •KOD Hot Start buffer ensures optimal PCr •performance
more information in novagen’s innovations newsletter 28 – order your free copy from your local VWR office.
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NEW!
when ordering KoD products listed below you will receive a free 2009 interactive
Pathways calendar.
order your DoPPio now, and take advantage of the
introductory offer. email your machine’s serial number to [email protected]
and we will send you a free of charge voucher from thermo
scientific Biopolymers on oligos to a value of 200 eur.
Additional 700€ in FrEE consumables including 2 hybridisation cassettes and 4 packs of 25 blank microarray slides. Choose the appropriate slide coating, amino or aldehyde modified, ideal for spotting PCr products and oligonucleotides; or epoxy or polymer modified slides, ideal for proteins.
Description cat. no. Price introductory priceDOPPIO Thermal Cycler 732-1210P 3.410,00€ 2.910,00€
Description size (mm) cat. no. Price Promo price*LuxScan™ 10K Microarray Scanner 360 × 540 × 220 737-0152P 24.890,00€ 19.912,00€
micro array slides Qty/Pk cat. no. PriceAmino coating 25 737-0097P 191,00€
Aldehyde coating 25 737-0101P 208,00€
Epoxy coating 25 737-0105P 196,00€
Polymer coating 25 737-0109P 159,00€
Cassette hybridisation gene chip 1 737-0112P 76,10€
Kits Preps cat. no. Price**Plasmid Midiprep 100 OMEGD6904-04P 325,00€
Plasmid Maxiprep 100 OMEGD6922-04P 486,00€
Fastfilter Midiprep 100 OMEGD6905-04P 427,00€
Fastfilter Maxiprep 100 OMEGD6924-04P 746,00€
Fastfilter Megaprep 20 OMEGD6929-03P 692,00€
Fastfilter Gigaprep 20 OMEGD6920-03P 971,00€
Product size cat. no. PriceKOD Xtreme™ Hot Start DNA Polymerase 200 U 71975-3P 495,00€
KOD Hot Start Master Mix 100 rxn 71842-3P 165,00€
KOD Hot Start Master Mix 500 rxn 71842-4P 660,00€
BioMarkeShop_12_DE.indd 1 10/10/2008 4:50:59 PM
Place your order today! visit our website http://de.vwr.comLocal price agreements apply.
offers valid from 1st november 2008 to 31st march 2009Promotions in the VWrbioMarke SHOP may not be used in combination, VWr reserves the right to refuse invalid orders.2
ISSUE 12 - NOVEMbEr 2008Shop!
AutovaxiD™ cell culture instrument increases mAb concentration by 50%AutovaxID™ is the next generation in automated cell culture production systems. All the instrumentation necessary for cell culture production is enclosed within a footprint of less than 0,3 m2. The simplicity and software automation allows the user to focus on cell production. Users go from box to operation in less than 5 minutes and can be fully efficient in just one day!! Product concentration in the AutovaxID™ is 10X to 30X higher than conventional cell culture methods. The unique two component design reduces the need for a clean room, as well as reduces the risk of process contamination.
reduces time by 80%•Takes only 20% of the space•reduced cost per mg of mAb by 60%•replaces 80 l stirred tank•
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Pcr mycoplasma test Kit The PCr Mycoplasma test kit is designed to detect the presence of mycoplasma contaminating biological materials, in for example cultured cells. Whereas mycoplasma detection by culturing can be difficult and time consuming, PCr testing gives results in a few hours.
Contaminant mycoplasma can be easily detected simply by verifying the bands of amplified DNA fragments in electrophoresis. There is no need to prepare probes labelled with radioisotopes, or to calculate enzyme, dNTP’s or buffer concentrations. Instead, a ready to use, optimised PCr mix is supplied.
The primer set allows detection of various mycoplasma species (M. fermentans, M. hyorhinis, M. arginini, M. orale, M. salivarium, M. hominis, M. pulmonis, M. arthritidis, M. bovis, M. pneumoniae, M. pirum and M. capricolum), as well as Acholeplasma and Spiroplasma species, with high sensitivity and specificity.
Simple, one-step reaction; less than 10 minutes to prepare the sample•Can be used routinely in every lab•Minimal sample handling reducing the risk of contamination•Sensitive (100 – 1000 CFU/ml)•M. hyorhinis• can be detected
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it’s time for 5 Prime’s perfect Pcr partnersthe easy way to your Pcr fragments – self adjusting Pcr buffer and accurate DnA fragment sizing.
combine the ultra-pure, thermostable recombinant 5 Prime Taq Polymerase with the new Perfectsize DnA molecular weight Ladders for optimal Pcr results.
5 PrIME Taq Polymerase enables you to get fragments from even the most specialised PCr applications while 5 PrIME PerfectSize DNA Molecular Weight Ladders allow accurate size determination and quantification of DNA fragments during electrophoresis. 5 PrIME Taq Polymerase features the self-adjusting Mg2+ buffer technology that automatically adjusts the Mg2+ concentration. This provides optimal conditions for any primer-template system, saving time and precious samples.
Five different 5 PrIME PerfectSize DNA Molecular Weight Ladders cover a wide range of fragment sizes at regular intervals and defined fragment concentrations. Each 600 µl vial is sufficient for 100 applications and is ready to load with a buffer containing three tracking dyes for optimal control of agarose gel run times.
electrophoresis
Cat. No. 733-0180P, Taq DNA Polymerase – 1000 UAmplification of fragments with different lengths using Taq DNA Polymerase.1. 160 bp fragment of apolipoprotein b (genomic DNA)2. 360 bp fragment of apolipoprotein b (genomic DNA)3. 400 bp fragment of Taq DNA Polymerase gene (60% GC, plasmid DNA)4. 547 bp fragment, GC rich (70% GC, genomic DNA)5. 10 kb fragment from the genome of lambda phage6. DNA Marker
Cat. No. 733-1794P PerfectSize 100 bp XL Ladder – 100 Produces eleven sharp bands from 100 to 1500 bp. Analysis on a 2.0% agarose gel.
three clicks away from pure DnA! DnA as simple as making coffee!
DNA extraction can’t get any simpler. GeneMole® is the small scale automated DNA extractor for every research lab. It is intuitive to operate and requires minimal preparation. Anyone can use it without training or special skills. best of all this system does not ruin your budget! Share your GeneMole® with colleagues or have one just for yourself.
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Adjustable slab gel kit16,5 cm wide and adjustable to a 16, 17, 19, 22 or 28 cm path length, this unit features adjustable upper reservoir, levelling base, platinum electrodes and safety-tip power leads. Includes 2 each 1,5 mm x 20 well combs, 2 each Gel Wrap™ gaskets, 2 sets of 22 cm glass plates, 2 sets of 1,5 mm thick spacers, 8 white spring clamps.
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*Incubator clean solution 500ml Cat. No. A5230.0500
track it! Barcoded 96 well Pcr platesAccurate tracking of both samples and data is essential in PCr/QPCr. To help you easily track and locate your samples, Abgene have extended their product range to include a barcoded 96 well PCr plate that fits the AbI fast block PCr and QPCr machines.
The thin walled design ensures that optimal and consistent heat transfer occurs, giving consistent high quality results. The barcode is a standard code 128 and is positioned on the opposite alpha side of the plate in the centre - the standard reading position for any of the AbI platforms.
Guaranteed to give you a perfect fit and like all Abgene plastics free from any contaminants such as DNA, rNA, DNase and rNase.
Code 128 barcoding is available on any skirted PCr plate, please enquire for further details. Sorry, not available in Italy, belgium and Netherlands
Buy 2 packs pay for only 1!
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The mixture contains everything (incl. Taq Pol.)
PAGer® Precast Gels for Protein electrophoresiseasy-to-load, easy-to-open, easy-to-try
PAGEr® Gels are easy-to-use protein minigels that offer sharper resolution, more consistent protein transfer, and longer shelf life than any other Tris-Glycine gel. PAGEr® Gels are easy to use and compatible with most minigel chambers.
See clearly for loading with our marked sample lanes •and tinted cassettesSnap open the cassettes with a simple twist of the •combrun PAGEr• ® Gels in your existing chamber
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The AutovaxID™ is 3 times more economical than traditional roller bottle or stirred tank methodsKey benefits:
Flexibility to choose from •1 to 16 samples/runJust three minutes handson time•Convenient prefilled and sealed reagent strips•Occupies minimal bench space•
* To claim simply email the serial number of the unit to [email protected] with your choice of reagents
order 5 Prime Taq and get a free vial of the Perfectsize 100bp XL ladder
Prosieve® colour Protein marker Free with the
purchase of any box of PAGer® Gels
Free 500 ml incubator clean solution *
For a full list of products or a free sample contact your local vwr sales office.
extra comb, spacer set, Gel wrap™ gasket and glass plate set Free.save over 65 euros.
1000 euros of free reagents with every
Genemole® instrument purchase*
Description cat. no. Price PCr Mycoplasma Test kit A3744.0020P 242,10€
Description range Bands content cat. no. PriceTaq DNA Polymerase 1000 U 733-0180P 215,00€
PerfectSize 50bp Ladder 50 – 500 bp 9 600 µl 733-1792P 91,80€
PerfectSize 100bp Ladder 100 – 600bp 6 600 µl 733-1793P 81,60€
PerfectSize 100bp XL Ladder 100 – 1500bp 11 600 µl 733-1794P 91,80€
PerfectSize 1kb Ladder 1kb – 10kb 9 600 µl 733-1795P 51,00€
PerfectSize 1kb XL Ladder 100bp – 10kb 12 600 µl 733-1796P 61,20€
Description Qty/Pk cat. no. Price GeneMole® instrument* 1 731-3051P 9.720,00€
MoleStrips™ DNA blood (test-kit) 32 731-3050P 110,00€
MoleStrips™ DNA blood 64 731-3053P 193,00€
MoleStrips™ DNA Tissue (test-kit) 32 731-3052P 132,00€
MoleStrips™ DNA Tissue 64 731-3054P 228,00€
Description cat. no. PriceAdjustable slab gel promo kit 730-1816P 655,00€
Description Qty/Pk cat. no. Price96 well fast block PCr plate with barcode 128 25 731-0136P 944,00€
96 well fast block PCr plate with barcode 128, white 25 731-0137P 1.030,00€
For more information contact your local vwr sales office.
BioMarkeShop_12_DE.indd 2 10/10/2008 4:51:25 PM
Place your order today! visit our website http://de.vwr.comLocal price agreements apply.
offers valid from 1st november 2008 to 31st march 2009Promotions in the VWrbioMarke SHOP may not be used in combination, VWr reserves the right to refuse invalid orders. 3
ISSUE 12 - NOVEMbEr 2008Shop!efficient fusion with BtXElectrofusion is a highly efficient, non-toxic technique suitable for nuclear transfer cloning, embryonic fusion, fusion or transfections of mammalian cells and plant protoplasts to name but a few applications. The ECM 2001 is a versatile system for both Electrofusion and Electroporation. Fusion can be viewed or manipulated under a microscope with the bTX Microslide chambers.
Incorporates both AC and DC (square) waveforms•Compatible with specialty electrodes for • in vivo applications
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cell Biology
Ac Parameters (Alignment) nonsinusoidal wave:
Frequency 1 MHz
Voltage 0 – 75 V (zero to peak)
Duration 0 – 99 sec
Post fusion AC 1/10 of pre-fusion amplitude
Post fusion ramp 1 – 9 sec
Pause between AC/DC 50μsec
Dc Pulse Parameters (Fusion) (electroporation) square wave:
High Voltage Mode:
Voltage (V) 10 – 3000
Pulse Length 1–99μsec
Low Voltage Mode:
Voltage (V) 10 – 500
Pulse Length 0,01 – 0,99 msec
resolution 0,01 msec
Number of Pulses 1 – 9
ultimate low cell binding culturewarenunc hydrocell™ Plates and Dishes for single-cell and cell-cluster suspension cultures.
The HydroCell product range consists of MicroWell plates, MultiDishes and Dishes with a super-hydrophilic polymer bound covalently to the culture surface, allowing suspension cultivation of single cells and cell clusters that otherwise adhere to tissue-culture treated and non-treated conventional cultureware.
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Examples of HydroCell applications: 1) Cultivation and analysis of macrophages and other monocyte-derived cell types; 2) Stem cell propagation (for example, neurospheres); 3) Stem cell differentiation (for example, embryonic stem cells to embryoid bodies); 4) Cultivation and analysis of tumor-cell spheroids; and 5) Analysis of cell secretions with minimal loss of sample to surface adsorption).
Licensed from CellSeed Inc. * Maximum one box of each product per customer
Discover the broad range of the new microplates from BrAnD for immunological applicationsModern research methods require high-quality disposables. brANDplates®, the new generation of 132 microplates from brAND can be used in a broad range of applications such as ELISA, rIA, FIA, etc. Three new immunological surfaces have been developed under the most modern production conditions.
immuno-treated (non-sterile)Depending on the characteristics of the molecules to be attached to the surface choose between these different types of polystyrene brANDplates® for solid phase assays:
immunoGrade™: Optimised for immobilisation of IgG (hydrophilic and hydrophobic regions), comparable to “high-binding” plates from other manufacturers
hydroGrade™: More hydrophilic than immunoGrade™, for the attachment of hydrophilic molecules like components of the complement system or glycoproteins
lipoGrade™: More hydrophobic (lipophilic) than immunoGrade™, for the attachment of hydrophobic molecules, for example progesterone and lipoproteins
immunoassay plates at a glance96 and 384 well formats•Standard, low volume or transparent •bottomTransparent, white or black•Different bottom shapes: U-, V-, F-, C-bottom for 96 well format•Clearly distinguishable by colour code: blue embossed alphanumeric coding for •96-well standard plates
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Buy now to save 30%
new nunc surface, introductory price
30% off
BD Primaria™ cell culture productsA unique nitrogen-containing surface chemistry for enhanced cell culture.
The gases used in bD’s vacuum-gas plasma process to manufacture bD Primaria™ Cultureware contain both oxygen and ammonia, resulting in the incorporation of a variety of nitrogen-containing functional groups in addition to the negatively charged oxygen-containing groups found on traditional TC surfaces. This incorporation of nitrogen-containing cations has been correlated to attachment and spreading of primary endothelial cells in a clonal cell-growth assay. The complex surface on bD Primaria™ is homogeneous and stable, and is used by researchers to improve attachment, spreading, growth and differentiation of a variety of cell types, including:
Hepatocytes•Transfected COS-7•Transfected HeK-293•
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up to 20% off for all BD Primaria
cellware products
When chick embryo spinal cord neurons are cultured on bD Primaria™, growth is enhanced and extensive neurite development occurs (b). In this experiment, cells clumped and detached from traditional TC plates (A) after 20 days in culture, but remained viable and differentiated on bD Primaria™.
CHO•Primary SMC and Skeletal Muscle Cells•Primary Cardiac Myocytes•
Osteoblasts•Neuronal Cells•Endothelial Cells and many more•
‘A’
‘B’
Please ask for samples! [email protected]
50% off microslides
Purchase an ecm 2001 and receive
a free pack of microslides
(0,5 mm gap)
Description Qty/Pk cat. no. Price Promo priceECM 2001 Embryo Manipulation System - Includes accessories for embryo manipulation 1 732-0615P 15.780,00€ -
ECM 2001 Electro Cell Fusion System - Includes accessories for cell fusion 1 732-0616P 18.110,00€ -
ECM 2001 Electroporation System - Includes accessories for electroporation 1 732-0802P 15.210,00€ -
ECM 2001 Generator only 1 732-0617P 14.630,00€ -
Microslides 450 0,5 mm gap 10 732-0004P 667,00€ 333,50€
Microslides 450-1 1 mm gap 10 732-0005P 667,00€ 333,50€
Microslide 453 3,2 mm gap 1 732-0006P 204,00€ 102,00€
Microslide 453-10 10 mm gap 1 732-0007P 204,00€ 102,00€
Description Lid sterile Pack cat. no. Price Promo price*MicroWell 96F Yes Yes 1/8 734-2245P 196,00€ 137,20€
MicroWell 96U Yes Yes 1/8 734-2246P 195,00€ 136,50€
MultiDish 24 Yes Yes 1/6 734-2247P 149,00€ 104,30€
MultiDish 12 Yes Yes 1/6 734-2248P 147,00€ 102,90€
Dish 10 cm Yes Yes 1/6 734-2249P 97,00€ 67,90€
Dish 6 cm Yes Yes 5/30 734-2250P 402,00€ 281,40€
Dish 3,5 cm Yes Yes 5/30 734-2251P 334,00€ 233,80€
Description no. of wells
well shape/volume (µl)
Qty/Pk cat. no. Price Promo price
immunoGrade™
Transparent 96 U/330 100 735-2042P 126,30€ 88,40€
Transparent 96 V/350 100 735-2043P 126,30€ 88,40€
Transparent 96 F/350 100 735-2044P 126,30€ 88,40€
Transparent 96 C/350 100 735-2045P 126,30€ 88,40€
White 96 U/330 100 735-2046P 250,50€ 175,30€
White 96 C/350 100 735-2047P 250,50€ 175,30€
black 96 U/330 100 735-2048P 250,50€ 175,30€
black 96 C/350 100 735-2049P 250,50€ 175,30€
black/transparent 96 F/320 100 735-2050P 610,50€ 427,30€
Transparent 384 F/100 50 735-2051P 207,40€ 145,10€
White 384 F/100 50 735-2052P 218,00€ 152,60€
black 384 F/100 50 735-2053P 218,00€ 152,60€
hydroGrade™
Transparent 96 U/330 100 735-2054P 151,60€ 106,10€
Transparent 96 F/350 100 735-2055P 151,60€ 106,10€
Transparent 96 C/350 100 735-2056P 151,60€ 106,10€
White 96 U/330 100 735-2057P 275,80€ 193,00€
White 96 C/350 100 735-2058P 275,80€ 193,00€
black 96 U/330 100 735-2059P 275,80€ 193,00€
black 96 C/350 100 735-2060P 275,80€ 193,00€
Transparent 384 F/100 50 735-2061P 249,50€ 174,60€
White 384 F/100 50 735-2062P 262,10€ 183,40€
black 384 F/100 50 735-2063P 262,10€ 183,40€
lipoGrade™
Transparent 96 U/330 100 735-2064P 212,60€ 148,80€
Transparent 96 V/350 100 735-2065P 212,60€ 148,80€
Transparent 96 F/350 100 735-2066P 212,60€ 148,80€
Transparent 96 C/350 100 735-2067P 212,60€ 148,80€
White 96 U/330 100 735-2068P 330,50€ 231,30€
White 96 C/350 100 735-2069P 330,50€ 231,30€
black 96 U/330 100 735-2070P 330,50€ 231,30€
black 96 C/350 100 735-2071P 330,50€ 231,30€
White/transparent 96 F/330 100 735-2072P 732,60€ 512,80€
black/transparent 96 F/330 100 735-2073P 732,60€ 512,80€
Transparent 384 F/100 50 735-2074P 300,00€ 210,00€
White 384 F/100 50 735-2075P 314,70€ 220,20€
black 384 F/100 50 735-2076P 314,70€ 220,20€
Transparent, low volume 384 F/30 50 735-2077P 389,50€ 272,60€
White, low volume 384 F/30 50 735-2078P 650,50€ 455,30€
black, low volume 384 F/30 50 735-2079P 600,00€ 420,00€
White/transparent 384 F/120 50 735-2080P 762,10€ 533,40€
black/transparent 384 F/120 50 735-2081P 762,10€ 533,40€
BD Primaria™ Petri Dishes
Description Qty/Pk cat. no. Price Promo price35 x 10 mm easy-grip dish 200 734-0070P 66,00€ 62,00€
60 x 15 mm standard dish 200 734-0071P 89,00€ 85,00€
100 x 20 mm standard dish 200 734-0072P 141,00€ 112,80€
BD Primaria™ Flasks
Description Qty/Pk cat. no. Price Promo price25 cm2 flask with canted neck plug-seal caps 200 734-0075P 181,00€ 144,80€
Vented, 0,2 µm caps 100 734-0073P 110,00€ 105,00€
75 cm2 flask with straight neck plug-seal caps 100 734-0076P 183,00€ 175,00€
Vented, 0,2 µm caps 100 734-0074P 192,00€ 185,00€
BD Primaria™ multiwell Plates
Description Qty/Pk cat. no. Price Promo price6 well multiwell plate with lid 50 734-0077P 115,00€ 99,00€
24 well multiwell plate with lid 50 734-0078P 123,00€ 99,00€
96 well multiwell plate with lid, flat-bottom 50 734-0079P 116,00€ 99,00€
BioMarkeShop_12_DE.indd 3 10/10/2008 4:51:58 PM
Place your order today! visit our website http://de.vwr.comLocal price agreements apply.
offers valid from 1st november 2008 to 31st march 2009Promotions in the VWrbioMarke SHOP may not be used in combination, VWr reserves the right to refuse invalid orders.4
ISSUE 12 - NOVEMbEr 2008Shop!
order from the customer service centre or your vwr Primary Distributor:These offers cannot be used in conjunction with any other pricing agreements from VWR International. Prices exclude VAT.
VWr International GmbHHilperstr. 20A64295 Darmstadt
Place your order today!*Tel: 0180-570 2000*Fax: 0180-570 2222http://de.vwr.come-Mail: [email protected]*14 Cent/Minute aus d. dt. Festnetzggf. abweichende Mobilfunktarife
simple, reliable concentrating and desalting with Pall nanosep devicesNanoSep centrifugal devices are ideal for rapid processing of samples of between 50 to 500 µl. The typical recoveries obtained using Nanosep devices are greater than 90%. Pall NanoSeps are constructed of low-binding polypropylene and contain the Omega membrane. The NanoSep range can be used for a number of applications including:
Concentrate, purify, and desalt oligonucleotides, •DNA and rNAClean up labelling and PCr reactions•Isolate DNA from agarose gel slices•Separate oligonucleotides and rNA from •acrylamide gelsSupplied in packs of 500•
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Buy 3 identical products and pay for only 2
nALGene® high-speed centrifuge tubes and bottles“Spin with confidence”
Designed for most common high-speed centrifuge rotors, the NALGENE® Oak ridge centrifuge tubes and bottles with screw closure are leakproof up to their maximum rated rCF (50 000 x g for tubes – refrigerated or not), giving you peace of mind when spinning your valuable samples down.
All NALGENE® centrifuge tubes are re-useable and made out of autoclavable plastics. Choose between polypropylene copolymer (PPCO) tubes for most common applications; crystal-clear polycarbonate (PC) tubes when visual clarity is desired; and Teflon FEP tubes for samples in aggressive organic solvents.
To find the tube or bottle that fits your rotor, contact your local VWr sales specialist.
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vwr collection incubating orbital shakerExclusive “Optiflow” forced ventilation system offers exceptional temperature uniformity. Microprocessor controls with digital displays for speed, temperature and time, plus user calibrations for temperature and speed. rS232 serial port for data logging and unit control.
Maximum load - 15,9 kg•Speed range - 15 to 500 rpm with an accuracy of +/- 1%•Temperature range - ambient +5 °C to 65 °C•Temperature uniformity is +/- 0,5 ºC at 37 ºC•Timer from 1 second to 9999 minutes•Orbit - 19 mm •
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€100 off the list price plus free platform for mounting flask clamps and test tube racks
2 ml cryogenic sterile cryule® vialsSpecially designed for safe effective cryogenic cell storage •Freestanding on the bench-lock into rack•External thread allows maximum vial capacity use and helps to avoid contamination•White marking area and colour-coded caps•Case of 500 Cryule• ® Vials packed in 10 sealed bags of 50 eachPolypropylene, dia x ht 12 x 46 mm•
sAve now… 20% oFF the reduced price!
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ready-to-use Dialysis DeviceSpectrum introduces the next generation (G2) in ready-to-use laboratory dialysis devices featuring Ultra-pure biotech Cellulose Ester (CE) Membrane.
95 – 98% sample recovery•Highest membrane purity•Superior handling & leak prevention•Volume specific dilution control•Complete & ready-to-use packaging•
the dialysis device with the best sample recovery, purity & dilution controlThe new Float-A-Lyzer® G2 is a low protein-binding synthetic membrane available in 4 precise MWCOs with no heavy metal and sulphide contaminants. Only G2 assures a 95-98% sample recovery while maintaining 99% sample purity and <1% sample dilution and is packaged dry.
promo code
21
Bigger pack size! Float-A-Lyzer® G2
comes in packs of 12 for the same price as the old pack of 10!
25% off
easy to use custom oligonucleotide online order tool!order now on www.vwr.com and mention the code vwr38 to claim your 1 GB-usB stick*Please contact your local VWr LifeSciences specialist for more information on how to order on the lab shop.
promo code
19
Free 1 GB-usB stick**terms and conditions:Offer only valid for orders placed from 1st November 2008 till 31st May 2009 through the VWr order page. Offer only available for the first 100 customers.Void when prohibited by law or company/ organisation.
S C I E N T I F I C
microplate readingmultiskan Fc, a reliable and easy to use microplate photometerThermo Scientific Multiskan FC is a new generation microplate photometer designed with 30 years experience in microplate photometry. Multiskan FC is a reliable and robust instrument for a wide variety of photometric research and routine applications offering ease of use via the large colour display, visual user interface and multiple language options.
Email [email protected] and supply us with the serial number of the instrument you purchased, state the wavelength of the filter you want, and we will send you your free filter.
promo code
22
Purchase a multiskan Fc and get an additional
filter* Free!
Contact your local VWr office and ask for a copy of the Nanosep Centrifugal Devices - Protocols for Use Guide PN 32989, or alternatively view the online version at www.pall.com/lab.
S C I E N T I F I C
Ask your local vwr sales office
*Offer valid for standard filters 340, 414, 492, 540, 550, 570, 595, 630, 650, 690 nm
nALGene Product Description size (ml) max. g-force at 4 °c Qty/Pk cat. no. Price Promo priceoak ridge centrifuge tube
PPCO (3119 type) 10 50 000 10 525-2241P 51,70€ 38,70€
PPCO (3119 type) 30 50 000 10 525-2243P 59,90€ 44,90€
PPCO (3119 type) 50 50 000 10 525-2244P 74,00€ 55,50€
PC (3118 type) 10 50 000 10 525-2231P 64,50€ 48,30€
PC (3118 type) 30 50 000 10 525-2233P 69,50€ 52,10€
PC (3118 type) 50 50 000 10 525-2234P 84,40€ 63,30€
PC (3118 type) 85 50 000 10 525-2235P 95,50€ 71,60€
FEP (3114 type) 10 50 000 2 525-2211P 45,60€ 34,20€
FEP (3114 type) 30 50 000 2 525-2212P 69,50€ 52,10€
FEP (3114 type) 50 50 000 2 525-2213P 77,60€ 58,20€
centrifuge Bottle with screw cap
PPCO (3120 type) 250 27 500 4 525-2311P 36,20€ 27,10€
PPCO (3120 type) 500 4 800 4 525-2313P 40,60€ 30,40€
PPCO (3120 type - Ht: 175 mm) 1000 7 100 4 525-2316P 83,10€ 62,30€
PPCO (3120 type - Ht: 168 mm) 1000 7 100 4 525-2315P 83,10€ 62,30€
PC (3122 type) 250 27 500 4 525-2331P 52,10€ 39,00€
PC (3122 type) 500 13 700 4 525-2333P 73,90€ 55,40€
PC (3122 type) 1000 7 100 4 525-2335P 111,00€ 83,20€
FEP (3127 type) 250 4 000 1 525-2341P 101,00€ 75,70€
Description cat. no Price Promo priceIncubating Orbital Shaker with an EN cord and 33 x 27,9 cm platform 444-7100P 2.610,00€ 2.510,00€
cap colour cat. no. reduced price Promo priceWhite 216-0395P 180,00€ 144,00€
red 216-3020P 180,00€ 144,00€
Pink 216-3021P 180,00€ 144,00€
Yellow 216-0398P 180,00€ 144,00€
Green 216-0401P 180,00€ 144,00€
blue 216-3022P 180,00€ 144,00€
sample vol. (ml) mwco (Daltons) cat. no. Price1
3 500 – 5 000
734-3573P 116,00€
5 734-3577P 126,00€
10 734-3581P 126,00€
1
8 000 – 10 000
734-3574P 116,00€
5 734-3578P 126,00€
10 734-3582P 126,00€
1
20 000
734-3575P 116,00€
5 734-3579P 126,00€
10 734-3583P 126,00€
1
100 000
734-3576P 116,00€
5 734-3580P 126,00€
10 734-3584P 126,00€
colour size (K) cat. no. Price
Grey 3 516-0584P 986,00€
blue 10 516-8492P 986,00€
red 30 516-8503P 986,00€
Clear 100 516-8521P 986,00€
Orange 300 516-8532P 986,00€
Description cat. no. PriceMultiskan FC microplate photometer (405, 450 and 620 nm filters included) 736-0355P 4.500,00€
Multiskan FC microplate photometer with incubator (405, 450 and 620 nm filters included) 736-0356P 6.680,00€
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