Table S1. The semi-conserved 7 nucleotide MEME motif of all selected and sequenced clones (30). The table includes the first 10 clones with the lowest p- value. The Initial start of the site is shown in the second column. The 10 clones are grouped in two sub-groups indicated by the underlined 6 nucleotide fully conserved sequence within the group.

Clone Start p-value Site

Clone 14 24 1.37e-04 ATTCAAA

Clone 2 26 1.37e-04 ATTCAAA

Clone 9 29 2.57e-04 ATTCAAC

Clone 20 21 3.29e-04 ATTCAAG

Clone 18 25 5.43e-04 ATCCAAA

Clone 12 58 5.43e-04 ATCCAAA

Clone 3 21 5.43e-04 ATCCAAA

Clone 1 61 5.43e-04 ATCCAAA

Clone 25 36 7.31e-04 ATCCAAC

Clone 17 60 7.31e-04 ATCCAAC

Table S1

Table S2. Rate constants calculated from Biacore sensograms of 400 nM RNA aptamers on amine coupled Protein E. 400 nM of each aptamer was injected at 10 µl/min on a Biacore CM5 chip with amine coupled Protein E (~1800 RU). The rate constants kobs and koff are the initial fast rates from a two-phase non-linear regression of 800 s of association and 800 s of dissociation. K D

is calculated as koff/kon. For the rate constants shown in the table the R-value of the two-phase non-linear regression is shown below the rate value. The koff for clone 1 and clone 23 that are underlined represents values with an R-value lower than 0.99 and no values could be obtained for clone 4 and the negative control d12.

koff (s-1)×10-2 kon(M-1s-1) ×105 kobs×10-2 KD (M)×10-8

Clone 1 2.27

(R=0.9663)Ambiguous Ambiguous No data

Clone 2 1.55 (R=0.9964)

1.29 3.61 (R=0.9970)

12

Clone 3 2.41(R=0.9961)

1.61 4.08(R=0.9947)

15

Clone 4 Ambiguous

Ambiguous Ambiguous No data

Clone 7 1.66(R=0.9965)

2.44 8.08(R=0.9970)

7

Clone 9 1.73(R=0.9952)

1.46 4.14(R=0.9970)

12

Clone 23 1.96(R=0.9928)

1.68 4.73(R=0.9895)

12

d12 Ambiguous

Ambiguous Ambiguous No data

Table S2

Figure S1

E-value 7.6e-004

Width 43

Sites 11

Figure S1. This figure shows a 43 nucleotide long motif isolated from a MEME run on all 30 random regions from the sequenced clones. The MEME motif analysis was run with no limitations on lenght. The presented motif was found in 11 clones.

― Clone 1― Clone 2― Clone 3― Clone 4― Clone 7― Clone 9― Clone 15― d12

Time (s)

Res

pons

e (R

U)

Figure S2. Binding of RNA aptamer on amine coupled PE in a Biacore. 400 nM of each aptamer was injected at 10 µl/min on a CM5 chip with amine-coupled PE (~1800 RU) on a Biacore 3000. The injection phase was 900 s and the dissociation phase was more than 1000 s. Data are Y-normalized using the Biaevaluation software.

Figure S2