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Anatomy and Anatomy and physiology of bacteriaphysiology of bacteria
1
UNIVERSITY OF UNIVERSITY OF SOMALIA (UNISO)SOMALIA (UNISO)
INTRODUCTIONINTRODUCTIONAll living cells can be classified into
two groups,ProkaryotesEukaryotes
Prokaryotes are structurally simpler and smaller than eukaryotes.
Prokaryotes cellsBacteria , Archaea
Eukaryotic cells.Plants ,animalsFungi (yeasts and molds),Protozoa, algae
INTRODUCTIONINTRODUCTION
Distinguishing characteristics of Distinguishing characteristics of prokaryotesprokaryotes
1. Their DNANot enclosed by a membraneSingular, circular chromosome
2. Their DNA is without histones
3. They lack membrane-enclosed organelles.
Distinguishing characteristics of Distinguishing characteristics of prokaryotesprokaryotes
4. Their cell walls contain the polysaccharide peptidoglycan.
5. They divide by binary fission. The DNA is copiedThe cell splits into two cells.
Prokaryotes = (from the Greek, meaning prenucleus)
Distinguishing characteristics of Distinguishing characteristics of eukaryoteseukaryotes
1. Their dnaFound in the cell’s nucleusSeparated from the cytoplasm by a nuclear membrane
Linear , multiple chromosomes
2. Their dna is associated with proteins called histones
Distinguishing characteristics Distinguishing characteristics of eukaryotesof eukaryotes3. They have a number of
membrane-enclosed organelles, including
1. Mitochondria2. Endoplasmic reticulum3. Golgi complex4. Lysosomes
4. Their cell walls, when present, are chemically simple.
5. Cell division involves mitosis
Eukaryote = from the Greek, meaning true nucleus
Distinguishing characteristics Distinguishing characteristics of eukaryotesof eukaryotes
CHECK YOUR UNDERSTANDING
Q1: What is the main feature that distinguishes prokaryotes from eukaryotes?
THE PROKARYOTIC CELLTHE PROKARYOTIC CELLVery small unicellular organisms
Prokaryotes include bacteria and archaea
The majority of prokaryotes are bacteria.
The species of bacteria are differentiated by many factors
a. Morphology (shape)b. Chemical composition (by
staining)c. Nutritional requirementsd. Biochemical activitiese. Sources of energy (sunlight or
chemicals).12
Co
cci b
acte
ria
bac
teri
a
Bac
illi b
acte
ria
Bac
illi b
acte
ria
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Sp
iral
bac
teri
a b
acte
ria
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External External StructuresStructures
Glycocalyx
Flagellum
Axial filaments
Fimbria
Pilus
The Size, Shape, and Arrangement of The Size, Shape, and Arrangement of Bacterial CellsBacterial Cells
Most bacteria range from 0.2 to 2.0 mμ in diameter and from 2 to 8 m in lengthμ
Bacterial basic shapes:
Coccus: (plural: cocci ) sphericalBacillus: (plural: bacilli) : rod-shaped
Spiral: hire-like
The Size, Shape, and Arrangement The Size, Shape, and Arrangement of Bacterial Cellsof Bacterial Cells
A) Cocci: Usually round or ovalElongatedFlattened on one side.
When cocci divide to reproduce, the cells can remain attached to one another.
The Size, Shape, and The Size, Shape, and Arrangement of Bacterial CellsArrangement of Bacterial Cells
Cocci that remain in pairs after dividing are called diplococci
Cocci that remain attached in chainlike patterns are called streptococci
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The Size, Shape, and Arrangement The Size, Shape, and Arrangement of Bacterial Cellsof Bacterial Cells
Cocci that divide in two planes and remain in groups of four are known as Tetrads
Cocci that divide in three planes and remain attached in cube-like groups of eight are called Sarcinae
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The Size, Shape, and Arrangement The Size, Shape, and Arrangement of Bacterial Cellsof Bacterial Cells
Cocci that divide in multiple planes and form grapelike clusters are called staphylococci
The Size, Shape, and Arrangement The Size, Shape, and Arrangement of Bacterial Cellsof Bacterial Cells
What is the medical What is the medical importance of theses cell importance of theses cell arrangements????arrangements????
helpful in identifying certain cocci bacteria!
This gives the likely diagnosis for the disease 29
◦B) Bacilli Single bacilli: most bacteria appear as single rods
Diplobacilli : appear in pairs after division
Streptobacilli: occur in chainsCoccobacilli : oval and look like cocci
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The Size, Shape, and The Size, Shape, and Arrangement of Bacterial CellsArrangement of Bacterial Cells
C) Spiral bacteria have one or more twists; they are never straight. Vibrios: Bacteria that look like curved rods
Spirilla: have a helical shape, like a corkscrew, and fairly rigid bodies.
spirochetes: spiral which are helical and flexible
The Size, Shape, and Arrangement of The Size, Shape, and Arrangement of Bacterial CellsBacterial Cells
Cocci, bacilli, and spirilla use flagella to move
spirochetes move by means of axial filaments
Resemble flagella but are contained within a flexible external sheath.
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The Size, Shape, and Arrangement The Size, Shape, and Arrangement of Bacterial Cellsof Bacterial Cells
CHECK YOUR UNDERSTANDINGCHECK YOUR UNDERSTANDING
How would you be able to identify streptococci through a microscope?
Why don’t bacilli form tetrads or clusters?
CHECK YOUR UNDERSTANDINGCHECK YOUR UNDERSTANDING
How do the planes of division determine the arrangement of cells?
CHECK YOUR UNDERSTANDINGCHECK YOUR UNDERSTANDING
What is the distinguishing feature of spirochete bacteria?
CHECK YOUR UNDERSTANDINGCHECK YOUR UNDERSTANDING
GlycocalyxGlycocalyxFound on external surface layer of some
bacteria Composed of sticky polysaccharides,
polypeptide, or bothTwo forms:Capsule:
Well organized Firmly attached to cell wall
Slime layer: Unorganized Loosely attached
Capsule Capsule of of bacteriabacteria
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Glycocalyx (capsule)Glycocalyx (capsule)
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1. Allows cells to attach → key to biofilm formation
2. Prevents phagocytosis → virulence factor
3. protect against dehydration.4. Its viscosity: inhibit the movement of
nutrients out of the cell.
E.g.: Bacillus anthracis, Streptococcus pneumoniae,
Streptococcus mutans
Flagellum – FlagellaFlagellum – FlagellaAre long filamentous appendages that move
bacteria (Motility)Attached to the cell wall and cell membrane
Flagella arrangements: Atrichous: Bacteria that lack flagella. (no
flagella) Monotrichous: a single flagellum at one
pole
Peritrichous: Flagella distributed over the entire cell
Lophotrichous: a tuft of flagella coming from one pole
Amphitrichous: flagella at both poles of the cell
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Flagellum – FlagellaFlagellum – Flagella
___________
_______
Flagellar ArrangementFlagellar Arrangement
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A flagellum has three basic parts 1.the filament, 2.the hook3.the basal body
MotilityMotility
Due to rotation of flagella
Mechanism of rotation: “Run and tumble”
Move toward or away from stimuli (taxis)
Chemotaxis , phototaxis magnetotaxis
Flagella proteins are H antigens (e.g., E. coli O157:H7)
Moving by flagellaMoving by flagella
““Run and Tumble”Run and Tumble”
Fig 4.9
Axial FilamentsAxial Filaments
Endoflagella
In spirochetes only
Anchored at one end of a cell
Rotation causes cell
to move
Fimbriae allow attachment
Pili are used to transfer DNA from one cell to another
Fimbriae and Pili
FimbriaeFimbriaeFor example, fimbriae on the
bacterium Neisseria gonorrhoeae the causative agent of gonorrhea, help the microbe colonize mucous membranes.
Once colonization occurs, the bacteria can cause disease.
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FimbriaeFimbriaeThe fimbriae of E. coli O157 enable this
bacterium to adhere to the lining of the small intestine, where it causes a severe watery diarrhea.
When fimbriae are absent (because of genetic mutation), colonization cannot happen, and no disease develops
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PiliPililonger than fimbriae only one or two per cell. Pili function in motility and DNA
transfersex pili are used to bring bacteria
together allowing thetransfer of DNA from one cell to
another, a process called conjugation51
Why DNA is transferred between Why DNA is transferred between two bacteria??? What is the two bacteria??? What is the advantage??advantage??
The exchanged DNA can add a new function to the recipient cell such as:
a.Antibiotic resistance b.The ability to digest its medium
more efficiently52
Cell WallCell WallRigid for shape & protection
⇒ prevents osmotic lysis
Consists of Peptidoglycan (murein) → polymer of 2 monosaccharide subunits ◦ N-acetylglucosamine (NAG) and ◦ N-acetylmuramic acid (NAM)
Functions of cell wallFunctions of cell wall
1. Surrounds the the fragile plasma membrane gives the bacteria shape and protection
2. prevent bacterial cells from osmotic lysis3. Contributes to the ability of some species
to cause disease 4. It’s site of action of some antibiotics5. Differentiate major types of bacteria.
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Fig 4.13
Linked by polypeptides (forming peptide cross bridges) with tetrapeptide side chain attached to NAM
Gram + Gram + Cell WallCell Wall
Thick peptidoglycan
Negatively charged Teichoic acid on surface
Thin peptidoglycan
Outer membrane
Periplasmic space
Gram – Cell Wall
Fig.4.13b
Gram-Positive Cell WallsGram-Positive Cell Walls
Teichoic acidsMay regulate movement of cations
Polysaccharides: provide antigenic variation
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60Cell wall of gram positive bacteria
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Cell wall of gram negative bacteria
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1- Lipid A: of LPS acts as endotoxin
Lipid A responsible for the symptoms associated with infections by gram-negative bacteria,such as:
• fever•dilation of blood vessels•Shock•blood clotting.
Gram-negative Cell WallGram-negative Cell Wall
2- The core polysaccharide: role is structural—provide stability 3- O polysaccharides functionsas an antigen and is useful for distinguishing species of gramnegativebacteria, e.g., E. coli O157:H7
LPS layer = outer layer of outer membrane
Gram neg. bacteria are less sensitive to medications??? Why????
Because the outer membrane acts as additional barrier !
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Gram-negative Cell WallGram-negative Cell Wall
Gram Stain MechanismGram Stain MechanismCrystal violet-iodine crystals form in
cell.
Gram-positive
◦ Alcohol dehydrates peptidoglycan
◦ CV-I crystals do not leave
Gram-negative
◦ Alcohol dissolves outer membrane and leaves holes in peptidoglycan.
◦ CV-I washes out
Bacteria with No Cell Wall: MycoplasmasBacteria with No Cell Wall: Mycoplasmas
Instead, have cell membrane with cholesterol compounds (sterols), similar to eukaryotic cells
Cannot be detected by typical light microscopy
This EM shows some typically pleomorphic mycoplasmas
Acid-fast Cell Acid-fast Cell WallsWalls
Genus Mycobacterium and Nocardia
mycolic acid (waxy lipid) covers thin peptidoglycan layer
Do not stain well with Gram stain → use acid-fast stain
Damage to Cell WallDamage to Cell WallLysozyme digests
disaccharide in peptidoglycan.
Penicillin inhibits peptide bridges in peptidoglycan.
Internal Structures: Internal Structures: Cell MembraneCell Membrane
Analogous to eukaryotic cell membrane:Phospholipid bilayer with proteins
(Fluid mosaic model)
Permeability barrier (selectively permeable)
Diffusion, osmosis and transport systems
Internal Structures: Internal Structures: Cell MembraneCell Membrane
Different from eukaryotic cell membrane:Role in Energy transformation
(electron transport chain for ATP production)
Damage to the membrane by alcohols, quaternary ammonium (detergents), and antibiotics causes leakage of cell contents.
Fig 4.14
Cytoplasm and Internal StructuresCytoplasm and Internal Structures
Location of most biochemical activitiesNucleoid: nuclear region containing
DNA (up to 3500 genes). Difference between human and bacterial chromosome?
Plasmids: small, nonessential, circular DNA (5-100 genes); replicate independently
Ribosomes (70S vs. 80S)
Inclusion bodies: granules containing nutrients, monomers, Fe compounds (magnetosomes)
EndosporesEndosporesDormant, tough, non-reproductive
structure; → germination → vegetative cells
Spore forming genera: __________?
Resistance to UV and γ radiation, desiccation, lysozyme, temperature, starvation, and chemical disinfectants
Relationship to disease
Sporulation: Endospore formation
Germination: Return to vegetative state
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EndosporesEndospores
Sporulation Sporulation
•Green endospores within pink bacilli•Many spores have already been released from the vegetative cells