The Endomembrane System Text and image sources are included using the notes function of the.ppt file

The Endomembrane System

Text and image sources are included using the notes function of the .ppt file

Cellular MembranesEndomembrane Structure % total membrane in

Hepatocyte% total in Pancreatic Exocrine Cell

Endoplasmic Reticulum (smooth)

16 <1

Endoplasmic Reticulum (rough)

35 60

Golgi 7 10

Plasma membrane 2 5

Lysosomes 0.4 N.D.

Peroxisomes 0.4 N.D.

Endosomes 0.4 N.D.

Mitochondria 39 21

Nuclear envelope (inner only)

0.2 0.7

Pulse-Label Autoradiography

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3 minutes 7 minutes

Pulse-Label Autoradiography

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37 minutes 117 minutes

The Endoplasmic Reticulum

DiOC6(3)

ER dynamics

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ER / Mitochondria

Video

“Smooth”ER

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Abnormal Pathology

sER from a chondrocyte of a patient with pseudoachondroplasia

Transition zones

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SER

RER

The rER

Supplying the NE

Movin’ on

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Note points of vesicle formation

Vesicular Traffic

donor membrane

recipient membrane

vesicle type

cell surface endosomes clathrin-coated

rER Golgi coatomerII-coated

Golgi Golgi coatomerI-coated

Golgi lysosomes clathrin-coated

Golgi cell surface coatomerI-coated

There are at least several (probably many more) distinct classes of vesicle coats/sorting mechanisms that function at different

places in the cell

coatomer-,smooth-coated vesicles

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Biochemical isolation of clathrin- coated vesicles

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Clathrin QuickTime™ and aTIFF (Uncompressed) decompressor

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Animation

Dynamin

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200 nm 100 nm

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shits wt

Breakin’ up is hard to do

Fusion

Just as there are specific mechanisms for sorting and vesicle formation, there are equally specific mechanisms for vesicle delivery

VSVGts::GFP transport

Vesicular stomatitis virus glycoprotein, temp-sensitive folding mutant

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Video

The Golgi

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Perinuclear Golgi

Biochemical stratification

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Post-Golgi transport tubules

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Video

Coatamer I Dynamics at the Golgi

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Fluorescence Recovery After Photobleaching (FRAP) of COPI::GFP

Lysosomes

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Exocytosis/Endocytosis

Peroxisomes

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