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DELIVERINGANEFFECTIVEJOURNALCLUBPRESENTATION
Amanda Preston, PhDScientific Editor
Children’s Foundation Research Institute [email protected]; 287 5368
INTRODUCTIONS
WHATISTHEPOINTOFJOURNALCLUB?
• Keepupwithscien-ficliterature
• Learnhowtocri-callyreviewscien-ficpapers
• Iden-fyareasofcollabora-onwithfellowJournalClubbers
• Shareknowledgeandideaswithscien-stsbothinandoutofyourfieldofresearch
• Learnhowtopresentyourideasandpar-cipateinscien-fic
debate
TALKOUTLINE:
Howtofulfillthetwo“jobs”oftheJournalClubpresenter
1.Selec-ngthepaper(s)topresent
2.Presen-ngthepapertotheJournalClubaudience
PresentaKonbasics
AudienceresponsibiliKes
JOB1:SELECTINGAPAPERTOPRESENT
" Selectthreescien-ficjournalar-clessuitableforpresenta-onandforwardthepdfstoDrAmaliSamarasinghe.
" DrSamarasinghewillhelpguideyourchoiceofar-cle
JOB1:SELECTINGAPAPERTOPRESENT
" Choose“good”papers-
" Peerreviewed
" Exploresandadvancesknowledgeaboutanimportantarea
" Approachestheproblemusinginnova-vemethodsandmul-pletechniques
" Mechanis-c,notpurelydescrip-ve
" Noreviews!Originalresearchonly
" Donotchooseanar-clethathaslotsofobviousflaws,purelyforthefactthatitwillbeeasytocri-cize
" Implica-onsformul-plefields
" WheretofindJournalClubar-cles:
" Fromyourownreadingoftheliterature,PubMed
" Colleagues,professors,friends
" Conferenceabstractbooks
" SciencePerspec-ves,NatureNewsandViews,Journal’ssocialmedia
" Reliablejournals-highimpactfactorjournals,nichejournalsforyourfield
JOB1:SELECTINGAPAPERTOPRESENT
" Yourjobistotellthestoryofthepaper
" Itiscri-calyouunderstandthepaperyouarepresen-ng
" Beprepared!
JOB2:PRESENTINGTHEPAPERTOJOURNALCLUBAUDIENCE
JOB2:PRESENTINGTHEPAPERTOJOURNALCLUBAUDIENCE
" InformaKontoinclude:
" Title,authorsandaffilia-ons
" Outlinethestory–givesufficientbackground
" Explicitlystatetheaims/majorques-onsofthepaper
" Explaintheresults,includingthemethodsused
" Statetheconclusionsofthestudyandputtheresultsintocontextofthelargerfield
" Commentonthevalidityofthemethods,resultsandtheconclusions
JOB2:PRESENTINGTHEPAPERTOJOURNALCLUBAUDIENCE
" InformaKontoinclude:
" Title,authorsandaffiliaKons
" Outlinethestory–givesufficientbackground
" Explicitlystatetheaims/majorques-onsofthepaper
" Explaintheresults,includingthemethodsused
" Statetheconclusionsofthestudyandputtheresultsintocontextofthelargerfield
" Commentonthevalidityofthemethods,resultsandtheconclusions
JOB2:PRESENTINGTHEPAPERTOJOURNALCLUBAUDIENCE
" InformaKontoinclude:
" Title,authorsandaffilia-ons
" Outlinethestory–givesufficientbackground
" Explicitlystatetheaims/majorques-onsofthepaper
" Explaintheresults,includingthemethodsused
" Statetheconclusionsofthestudyandputtheresultsintocontextofthelargerfield
" Commentonthevalidityofthemethods,resultsandtheconclusions
OUTLINETHESTORY-GIVESUFFICIENTBACKGROUND
" Howdidyoucomeacrossthisar-cle?
" Whatinforma-ondoestheaudienceneedtounderstandthisstudy?
" Whyisthestudyimportant?
" Rememberthatnoteveryoneisfromyourfieldofresearch
" Avoidjargon
" Beguidedbytheinforma-onintheIntroduc-on
SOS
ENaC
PIP3
C3G
PIP3
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GAB1
Insulin
PDK1/2
PKCα Akt
p70
PKC
GAGp85
JAK
NIK
ppPI3K
Akt
Tsc1
SOCS3Shc
NINIKKNNGRB2GRB2
IRS-1Fyn
GRB10
Ras
c-Raf
MEK1/2
ERK
PDE3B
PKA
SGK
JNK
SHP2
IRS-1
PTENPTEN
SHIPCAPCbl
PPFlotillinn
Cav
blAPSSynip
ASIPPP2A
Bad
mTOR
p70 S6K
4E-BP1
eIF4BeIF2BI
ATP citratelyase
PP1
PP GlycogenSynthase
RaptorGSK3
Akt SGK
T 1T 1
Tsc2
22
FynF
Crk
FKHR AFXX FKHR1 ERK
C3CC3CrKII
TC10TC10TC10
CIP4/2
P
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P
P P P
Glucose SNAREComplex
Lipid Rafts
GLUT-4
InsulinReceptor
AminoAcids
ATP
GLUT-4Vesicle
cAMPStress
SodiumTransport
LipolysisGrowth
GlycogenSynthesis
Fatty AcidSynthesis Protein
SynthesisApoptosis
Apoptosis
GLUT-4 Translocation
Key
Kinase
Phosphatase
TranscriptionFactor
Ligand
Insulin pathway
Discover more at abcam.com/insulin
• Redraw if necessary
IR
IRS1
PI3K
PIP3
Cav APS
SIMPLEISBETTER!
JOB2:PRESENTINGTHEPAPERTOJOURNALCLUBAUDIENCE
" InformaKontoinclude:
" Title,authorsandaffilia-ons
" Outlinethestory–givesufficientbackground
" Explicitlystatetheaims/majorquesKonsofthepaper
" Explaintheresults,includingthemethodsused
" Statetheconclusionsofthestudyandputtheresultsintocontextofthelargerfield
" Commentonthevalidityofthemethods,resultsandtheconclusions
EXPLICITLYSTATETHEAIMS/MAJORQUESTIONSOFTHEPAPER
" Fairlyself-explanatory
" UsuallyfoundintheIntroduc-onofthepaper
" Helpstodrawbackinaudiencememberswhomayhavedridedoff
" Givesyoua“measurings-ck”toassessifthestudyachievesitsstatedaims
" Itisfinetostatefindingsrightatthestartofyourpresenta-on
JOB2:PRESENTINGTHEPAPERTOJOURNALCLUBAUDIENCE
" InformaKontoinclude:
" Title,authorsandaffilia-ons
" Outlinethestory–givesufficientbackground
" Explicitlystatetheaims/majorques-onsofthepaper
" Explaintheresults,includingthemethodsused
" Statetheconclusionsofthestudyandputtheresultsintocontextofthelargerfield
" Commentonthevalidityofthemethods,resultsandtheconclusions
PRESENTINGFIGURES
" Onlinear-cles-downloaddirectlyintoPowerpoint.ForPDFfiles-AdobePro
" Whenresizingfigures–donotstretch
" SlideTitle=findingofthefigure
" Allfiguresshouldfillscreenandbereadablefromthebackoftheroom
" Mul--partorcomplicatedfigures Splitupondifferentslides Useanima-onto“build”complicatedslides
PRESENTINGFIGURES
" Alwaysdescribetheassayorexperimentfully
" YoudonothavetopresentEVERYfigureortable
" Forcomplexstudies,simplifyresultswithbasicillustra-on
PRESENTINGTABLES
" Makesurethetablesarelegible Considerre-typing
" Forverylargedatatables,re-typeonlytheimportantinforma-on
" SimplifywherepossibleRoundoffnumbersUsecolorstoshowrela-veincreasesordecreases
SUMMARIZECOMPLEXDATAINTOSIMPLEDIAGRAMS
TheCancerGenomeAtlasResearchNetwork(2017)IntegratedgenomiccharacterizaKonofoesophagealcarcinomaNature(2017)
PeyserandGrandis(2017)Cancergenomics:SpotthedifferenceNatureNewsandViews
SUMMARIZECOMPLEXDATAINTOSIMPLEDIAGRAMS
METHODS
" Giveabriefoutlineoftechniqueswherenecessary
" Rememberyouraudiencehasadiversescien-ficbackground
" Donotforgetsta-s-calanalysis
JOB2:PRESENTINGTHEPAPERTOJOURNALCLUBAUDIENCE
" InformaKontoinclude:
" Title,authorsandaffilia-ons
" Outlinethestory–givesufficientbackground
" Explicitlystatetheaims/majorques-onsofthepaper
" Explaintheresults,includingthemethodsused
" Statetheconclusionsofthestudyandputtheresultsintocontextofthelargerfield
" Commentonthevalidityofthemethods,resultsandtheconclusions
– Whichconclusionsaredirectlydrawnfromtheanalysisoftheresults,andwhicharemorespecula-ve?
– Doalloftheconclusionsdrawnmakesensebasedontheresults?
– Hasthisar-clesupportedthegenerallyacceptedthinkingonthistopicorhasitrefutedit?
– Howhasthisar-clefurtheredthinkinginthefield?
STATETHECONCLUSIONSOFTHESTUDYANDPUTTHERESULTSINTOCONTEXTOFTHELARGERFIELD
hlp://evolu-on.berkeley.edu/evolibrary/teach/journal/journal-ps.php
JOB2:PRESENTINGTHEPAPERTOJOURNALCLUBAUDIENCE
" InformaKontoinclude:
" Title,authorsandaffilia-ons
" Outlinethestory–givesufficientbackground
" Explicitlystatetheaims/majorques-onsofthepaper
" Explaintheresults,includingthemethodsused
" Statetheconclusionsofthestudyandputtheresultsintocontextofthelargerfield
" Commentonthevalidityofthemethods,resultsandtheconclusions
CRITIQUE
• Givetheauthorscreditforelegantexperiments
• Beforebeinghighlycri-cal,considerthelimita-onsweallworkunder
• partofcri-calanalysisisunderstandingandapprecia-nggoodexperimentaldesign
CRITIQUE" Peer-reviewgenerallydoesaprelygoodjobofweedingout“badscience”–howevertheys-llexist!
" Lookfor
" Sensa-onalistheadlines
" Misinterpretedresults
" conflictsofinterest
" Correla-onconflatedwithcausa-on
" Specula-velanguage
" Samplesizetoosmall
" Inappropriatesta-s-calanalysis
ParsonsECMandWrightAJ(2015)Thegood,thebadandtheuglyscience:examplesfromthemarinesciencearena.Front.Mar.Sci.2:33.
" Unrepresenta-vesamples
" Nocontrolorinappropriatecontrol
" Noblindtes-ng
" “Cherry-picked”results
" Unreplicatedresults
" Hasthepaperbeencited?
CRITIQUE• Arealloftheresultsobtainedconsistentwiththehypothesisbeing
tested?Arethereanymajoroutliersinthedata?
• Whatsortofevidencewouldmaketheauthors'casestronger?
• Whatsortofevidencewouldargueagainsttheauthors?
• Whatcasewouldaskep-calscien-stmakeagainsttheauthors'interpreta-onoftheirresults?
hlp://evolu-on.berkeley.edu/evolibrary/teach/journal/journal-ps.php
QUESTIONSANDDISCUSSION
" Ques-onsfromtheaudiencewillbedirectedtothepresenteratfirst–bepreparedtofieldques-ons
" Youarenotexpectedtoknoweverythingaboutthepaper
PRESENTATIONBASICS
" arrivetotheloca-onatleast15minutesearlytogetsetuponthecomputer
" Haveyourpresenta-ononaflashdrive
" Alaserpointerwillbeprovided
• Prac-ceyourpresenta-onoutloud
• Speakinaloudclearvoice,donotrushthroughyourslides
• speakerevalua-onsheetasguide
PRESENTATIONBASICS
• Keepslidesuncluleredwithlogosandfancybackground
• Makesureyourtextcontrastswiththeslidebackground–egblacktextonawhitebackground
• Textshouldbeatleast26ptandsanserif
• Bolding,colorandspacingcanbeusedforemphasis
AUDIENCEMEMBERS:READTHEPAPER!!
" Youareexpectedtopar-cipateintheJournalClubdiscussion
" Asyoureadthepaper,askthesameques-onsthatthepresentermight
" PleasebepreparedtoexchangeideasinJournalClub
QUESTIONS?
