NEW SERIES SUBSCRIPTION, $6.00VOL. 83, No. 2164 FRIDAY, JUNE 19, 1936 SINGLE, COPIES, .15

Goodell* ~~~~~~~~Library

JUN13 193Massachus(State Colle

TO REISEAR'CHACCOMPLISHMENTThe 25o,000th microscope manufactured by B & L, aDDE, was awarded to Dr. Frederick George Novyfor his contributions to science at the AAAS meetingat Rochester, N. Y.

Microscope DDE incorporates the latest developments in research microscopedesign and is fully equipped to perform any type of research microscopy. Yet thissweeping statement does not do justice for it omits the many features contributingto your convenience and comfort.Starting with the inclined binocular body tube, most comfortable for extendedobservations, you find such conveniences as front arm location which gives freeaccess to object, stage, objectives, substage and mirror. Counter-balanced rackand pinion, and extra long fine adjustment operating heads are added conveniences.Stage operation is extremely handy. Substage with patented supplementary con-denser on swing arm is another time saver.These and many other conveniences make Microscope DDE the ideal time andlabor saving companion for your research hours. Write for catalog D-I64.Bausch & Lomb Optical Co., 642 St. Paul St., Rochester, N. Y.

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SCIENCE-ADVERTISEMENTS VOL. 83, No. 2164

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JUNE 19, 1936 SCIENCE-ADVERTISEMENTS

Ready summer 1936-A modern text-book for undergraduate courses in theconservation of natural resources

OUR NATURAL RESOURCESAND THEIR CONSERVATION

A. E. PARKINS, Editor-in-ChiefProfessor of Geography, George Peabody College for Teachers

J. R. WHITAKER, Associate EditorAssistant Professor of Geography, University of Wisconsin

and 20 other contributors

It is the purpose of this book to survey the field of conservational theory and practice fromthe geographic viewpoint. Particular attention is given throughout to the extent and distribu-tion of our natural resources, and to their service in regional and national development; eachproblem discussed is sharply localized, being viewed as the outgrowth of natural and culturalconditions in specific parts of our country;_emphasis is placed on both the natural and thehuman factors involved in the genesis of current problems; and each problem is viewed notin isolation but in its relation to other problems associated with it.

The central purpose of the book is to give a balanced, concrete view of resources and problemsin their actual setting as a part of the organic units involved, whether regions, states, or thenation. Such a survey must depend, of course, on contributions from specialized fields, such as

geology, botany and engineering, as well as from the more general fields, such as geography,economics and history. This method of presentation is therefore reflected in the varied author-ship shown in the table of contents.

Specifically, "Our Natural Resources and Their Conservation" covers general aspects of con-servation in America; problems of the conservation of land, forests, water and water power,mineral resources, wild life, etc.; methods of handling these problems by local, state or nationalaction; the relation between the conservation of natural resources and the manufacturingindustry; recreational resources, and the conservation of man.

Among those contributing to this book may be listed: Wallace W. Atwood Sr., S. S. Visher,Louis A. Wolfanger, H. H. Bennett, J. Russell Smith, Ralph H. Brown, V. E. Shelford, H.C. Hanson, George J. Miller, 0. E. Baker, J. R. Whitaker, Robert M. Brown, Frank E.Williams, A. E. Parkins, Guy Harold Smith, William H. Haas, Nels A. Bengtson, Helen M.Strong, Edward C. Prophet, Ellsworth Huntington, Loyal Durand Jr., and George T. Renner.

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4 SCIENCE-ADVERTISEMENTS VoL. 83, No. 2164

VOL. 83, No. 2164

Summit Hill fire barrier and stripping was the lastcoal property visited, after which the party went tothe carnotite locality in the Pottsville conglomeratenear Mauch Chunk, at which point they disbanded.The trip was planned and conducted by the staff

of the department of geology, Lehigh University,with the hearty assistance and cooperation of variouscoal companies.

LAWRENCE WHITCOMB,Secretary

IN HONOR OF PROFESSOR CARLSONON June 1 a portrait bust of Dr. Anton J. Carlson,

prepared by Mrs. Alice Littig Siems, was presentedto the University of Chicago by students, colleaguesand friends of Dr. Carlson. The unveiling and pres-entation speech was made by Dr. A. B. Luckhardt,who paid the following tribute to his colleague:

It is unfortunate, perhaps, that I, who probably knowProfessor Anton Julius Carlson better than any one inthis audience, should have been called upon to present tothe University of Chicago, through its vice-president, thisportrait bust about to be unveiled. I think I knowenough of medical history, with its lore and legends,to thoroughly realize that many of the past worthies havebeen glorified much beyond their merited deserts. I donot propose to make the same mistake by a deluge ofencomiums and reflection on a great man and one witha marvelous career. Even if he were here with us, Ishould say the same of him. He would not want it other-wise from me.Many years ago, Anton Julius Carlson came from

Sweden as a lad of fourteen-a non-descript immigrant.America gave him an opportunity for a fruitful life ofscientific and civil endeavor and influence. He seized theopportunity and made the most of it. First at StanfordUniversity, next at Pennsylvania and then at Chicago,he pursued biological truths on the basis of intense ex-perimental efforts. Throughout a long life time, he haspracticed the principle that facts must be ascertainedby observation and not by divination. After that, logicand philosophy might well toy with them. Like his il-lustrious predecessors, he used a variety of gadgets toget at the facts; and like a Claude Bernard and a CarlLudwig he wove a logical and philosophical fabric aboutmany of them. But, he would be the first to discount theimportance of the philosophic fabric, and also the firstto emphasize the importance of the facts.

Establishing for himself an enviable record at thisuniversity as a great teacher and investigator, he at-tracted numerous students. Many a recipient of a higherdegree from this university (and one well earned under

Dr. Carlson 's guidance) now holds an important post inthis country. In fact, I doubt whether any departmentalchairman at this university or abroad, past or present,could adduce a greater scientific progeny than he-withthe possible exception of the immortal Carl LudwigI

Aside from a warped judgment on the scientific worthof former students and adventurers in science, born ofextreme personal loyalty, he has distinguished himselfamong his medical and scientific colleagues by an uner-ring judgment based on astute and critical analysis of thepresented data. That is why potential contributors toscientific and medical journals have sought his counselin advance, to avoid his straight-from-the-shoulder andtrenchant criticism on the floor of any scientific gather-ing.

Little wonder then that seientific bodies of all kindshave elected him to work in their behalf and have grantedhim honors. He is on all occasions an honored, respectedand inspiring guest. His admirers here and abroad arelegion I

I have the honor and the rare privilege to present toyou, Mr. Woodward, as representative of the university,the portrait bust of Professor Anton Julius Carlson,living and active chairman of the department of physi-ology.

Vice-president Woodward, in receiving the gift onbehalf of the university, also paid tribute to Dr. Carl-son's accomplishments and expressed, on behalf of the.president and board of trustees, their great apprecia-tion of hi.s long service to the university.

RECENT DEATHSDR. JOHN H. HAMMOND, consulting mining engi-

neer, died on June 8 at the age of eighty-one years.DR. Juiius A. NImUWLAND, professor of chemistry

at Notre Dame University, died suddenly on June 1LHe was fifty-eight years old.

DR. CHARLES E. JOHNSON, head of the departmentof forest zoology and director of the Roosevelt WildLife Forest Experiment Station at the New YorkState College of Forestry, died on June 6, at the ageof fifty-six years.

DR. ALBERT B. REAGAN, special professor of anthro-pology at Brigham Young University, died on May 30at the age of sixty-five years. Before joining the staffof Brigham Young University in 1934 he had servedfor thirty-four years in the U. S. Indian Service.THuRAL DALE FOSTER, a promising young student of

Mollusea, died of Hodgkin's disease on June 6 afterhaving completed all requirements for the doctorate inzoology in the University of Illinois. He would havereceived his degree on June 8.

SCIENTIFIC NOTES AND NEWST= honorary degree of doctor of laws, the only Joseph Sweetman Ames, president emeritus. The

honorary degree given at the commencement of the degree was conferred in absentia, owing to the illnessJohns Hopkins University, was conferred on Dr. of Dr. Ames.

592 SCIENCE

VOL. 83, No. 2164

In addition to promoting proliferation of fibro-blasts, this medium causes growth of muscle cells andepithelial cells. Fragments of fresh heart produced aconsiderable mass of actively pulsating tissue whenthey were cultivated in this medium. Iris epitheliumfrom a chick embryo proliferated in the medium for65 days.When the medium was used in the Lindbergh5 ap-

paratus for the cultivation of whole adult- organs,6 thefollowing interesting results were obtained. Ovariesof the adult cat tripled their weight in 5 days. Thy-roids doubled their weight in 3 or 4 days. The epi-thelial cells proliferated within and also outside thefollicles. The follicles also increased in number to amarked extent. The glucose consumption of theorgans was considerably greater than that of organscultivated in 40 per cent. serum. Thus, cat thyroidscultivated in the artificial medium metabolized 17 mg ofglucose daily, whereas those cultivated in serum metab-olized only 7 mg daily. After the thyroids had beencultivated for some time in this medium, they weresectioned, and fragments from them were cultivatedaccording to the usual techniques. These fragmentsgrew as actively as do fragments of embryonic thyroid.The medium designed for the cultivation of mono-

cytes has the following composition:

For cells cultivatedin a fluid medium

euer 100 ccSerum ........ 25.00 .....ccWitte's pep-

tone* ...... 85.00 mg

VitaminA4 . .50.00 to 100.00 units

VitaminD4 .. 1.00 to 2.00 Its

Vitamin Bi' ... 0.0053 "

Vitamin B27 ... 0.0001 "Vitamin C (crys-

talline ascor-bic acid) ... 0.085 mg

Glutathione ... 0.34 "Cysteine hydro-

chloride .... 1.125 "

Hemin ....... 0.00045 "

Insulin .0.012 unitsThyroxine ....... 0.000113 mgPhenol red .... 5.00 "

Glucose .. . 200.00 "

Sodium chloride 581.00 "

Potassium chlo-ride ........ 15.00 "

Calcium chloride,anhydrous . . 15.00

Magnesium chlo-ride, 6 H20 7.50

Sodium dihydro-gen phosphate 3.75

Sodium bicarbo-nate ....... 75.00 "

For cells cultivatedin a coagulumper 100 co

25.00 cc

170.00 mg

100.00 to 200.000 units

2.00 to 4.00 "

0.0106 "

0.0002 "

0.17 mg0.68 "

2.25 "

0.0009 "

0.024 units0.000225 mg5.00 "

200.00 "

581.00

15.00 "

15.00 "

7.50 "

3.75 "

75.00 "

* Monocytes proliferate more rapidly in tryptic digestsof fibrin than they do in Witte 's peptone.8 Such digestsmay be substituted for the peptone. The peptone hasbeen used here because of the greater ease with which themedium can be reproduced.

5 C. A. Lindbergh, Jour. Exp. Med., 62: 409, 1935.6 A. Carrel and C. A. Lindbergh, SCIENCE, 81: 621,

1935.7 Obtained from a concentrate prepared by Burroughs

Weilcome and Company.8 L. E. Baker, Jour. Exp. Med., 57: 689, 1933.

When the medium is used for organ cultivation, theglucose is increased to 300 mg per cent., and thesodium chloride reduced sufficiently to keep the solu-tion isotonic.

Chicken monocytes have been cultivated in thismedium for 80 days. At first, they proliferated soactively as to cover the entire area of the flask infour days. Half of- the cells were then removed. Inanother three days, the flask was again covered withcells. In order to prevent overcrowding, a portion ofthe cells was removed every four or five days for atleast a month. After that, proliferation was not sorapid. It continued, however, throughout the entire80 days of cultivation. Control cells that were culti-vated in 25 per cent. serum without any of the otherconstituents of the medium proliferated very slowly.It was not necessary to remove any cells to preventovercrowding during their entire time of cultivation.9The quantity of serum used in the medium may be

varied to a considerable extent, according to the re-sults desired. When it is increased to 50 per ceht.proliferation is still more rapid. When it is reducedto 15 or to 10 per cent., the cells proliferate lessrapidly. It can not be eliminated altogether. With aslow a concentration as 10 per cent. serum, the mediumsustained the proliferation of monocytes for 62 days.Control cells cultivated in 10 per cent. serum andTyrode solution, without the other ingredients of themedium, died in 12 days. It is evident, therefore, thatthe constituents used with the serum have a true nu-tritive value, and can replace serum to a considerableextent. This medium is now being used with suceessfor the cultivation of whole adult spleens in the Lind-bergh apparatus.To summarize: Artificial media have been developed

that cause fibroblasts, epithelial cells and monocytes toproliferate rapidly. Although still incomplete, andneeding serum as one constituent, they allow the cul-tivation of cells for considerable periods of time. Forthe present, they are the most efficient artificial mediaas yet devised for the cultivation of tissues and entireorgans.

LILLIAN E. BAKERTHE ROCKEFELLER INSTITUTEFOR MEDICAL RESEARCH

9 The controls were kept for only a month.

BOOKS RECEIVEDCLARK, J. G. D. The Mesolithic Settlement of NorthernEurope; A Study of the Food-Gathering Peoples ofNorthern Europe during the Early Post-Glacial Period.Pp. xvi + 284. 74 figures. 8 plates. Cambridge Uni-versity Press, Macmillan. $9.00.

HULL, GORDON F. An Elementary Survey of ModernPhysics. Pp. xxiv + 457. Illustrated. Macmillan. $4.50.

PENNELL, VERNON. A Handbook of Urology for Stu-dents and Practitioners. Pp. viii + 224. 34 figures.Cambridge University Pre, Macmillan. $2.75.

606 SCIENCE

SCIENCE-ADVERTISEMENTS

The American Journal of AnatomyPublished by The Wistar Institute

Charles R. Stockard, Managing Editor, Cornell Univ. Med. School

Vol. 59 Contents for July, 1936 No. 2H. H. COLE. On the biological properties of mare gonadotropic hormone. Four plates.

B. CAMPBELL. The comparative myology of the forelimb of the hippopotamus, pig and tapir. Seven textfigures.

E. M. TENCi. Development of the anus in the human embryo. Two plates.

E. J. CAREY. Studies in the wave-mechanics muscle form and funetion. II. The experimental biophysicsof the external form and internal structure of cross-striated muscle and tendon. Seven plates.

H. E. JORDAN. The relation of lymphoid tissue to the process of blood production in avian bone marrow.Seven plates.

Price $7.50 per volume, Domestic; $8.00 per volume, ForeignIssued bimonthly

Many early volumes are still available. Prices upon request

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THE IDENTITY THEORYBy

Blamey Stevens

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Sherratt & Hughes,Manchester, Eng.

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BIOLOGICAL STAINSBy H. J. CONN

A Handbook on the Nature and Usesof the Dyes Employed in the

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pages in length, or about 50 pages larger than the2nd edition. AU information has been brought upto date and descriptions of 23 new dyes and theirbiological applications are given. This book, be-sides giving certain laboratory procedures, treatsthe chemistry of dyes from the standpoint of thebiologist. Published by the Book Service of Bio-logical Stain Commission.

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JUNEM 19, 1936 5

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