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FOR TECHNICAL SUPPORT | Tel +1.608.441.2852 | www.TheTransfectionExperts.com Ingenio ® Electroporation Solution & Kits Save on Cost Without Compromising Your Results Product Cost/Electroporation* Savings Amaxa ® Nucleofector ® Kit V (VCA-1003) - Ingenio ® Electroporation Kit (MIR 50112) 42% *Based on list prices from company websites and protocol recommendations (25 RXN Kit). Figure 1. Cells were electroporated in parallel with an EGFP reporter vector and assayed at 24 hours post-electroporation by flow cytometry. Two electroporators were used with different electroporation solutions: the Ingenio ® Electroporation Kit was used in the Gene Pulser Xcell™ Eukaryotic System (Bio-Rad) or the Amaxa Nucleofector II Device (Lonza); the Amaxa Nucleofector Kit V was used in the Amaxa Nucleofector II Device, all according to manufacturers’ recommendations. Ingenio Provides Comparable Efficiency on Amaxa’s Nucleofector Device Prove it to Yourself − Try a FREE Sample of Ingenio Solution Today! High Efficiency Electroporation x Save money and reduce research costs while maximizing results x Compatible with conventional electroporation devices including Lonza-Amaxa ® , Bio-Rad ® or Harvard BTX ® x Ideal for plasmid or siRNA electroporation x High efficiency electroporation of hard to transfect cell lines and primary cells 0 20 40 60 80 100 % EGFP Positive Cells HL-60 K-562 Jurkat E6-1 SK-N-MC Ingenio ® Solution using Gene Pulser Ingenio ® Solution using Amaxa ® Nucleofector ® Amaxa ® Nucleofector ® Solution V on Amaxa ® Nucleofector ®

High Efficiency Electroporation

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Ingenio® Electroporation Solution & Kits
Amaxa® Nucleofector® Kit V (VCA-1003) -
Ingenio® Electroporation Kit (MIR 50112) 42% *Based on list prices from company websites and protocol recommendations (25 RXN Kit).
Figure 1. Cells were electroporated in parallel with an EGFP reporter vector and assayed at 24 hours post-electroporation by flow cytometry. Two electroporators were used with different electroporation solutions: the Ingenio® Electroporation Kit was used in the Gene Pulser Xcell™ Eukaryotic System (Bio-Rad) or the Amaxa Nucleofector II Device (Lonza); the Amaxa Nucleofector Kit V was used in the Amaxa Nucleofector II Device, all according to manufacturers’ recommendations.
Ingenio Provides Comparable Efficiency on Amaxa’s Nucleofector Device
Prove it to Yourself − Try a FREE Sample of Ingenio Solution Today!
High Efficiency Electroporation x Save money and reduce research costs while maximizing results
x Compatible with conventional electroporation devices including Lonza-Amaxa®, Bio-Rad® or Harvard BTX®
x Ideal for plasmid or siRNA electroporation
x High efficiency electroporation of hard to transfect cell lines and primary cells
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20
40
60
80
100
% E
Amaxa® Nucleofector® Solution V on Amaxa® Nucleofector®
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FOR TECHNICAL SUPPORT | Tel +1.608.441.2852 | www.TheTransfectionExperts.com
www.TheTransfectionExperts.com
PRODUCT NO. SIZE
PRODUCT NO. SIZE
*Primary cell types
Figure 2. Ingenio® Electroporation Kits were used to transfect representative cell types using Amaxa® Nucleofector®. Cells were assayed at 24 hours post-electroporation by flow cytometry and reported as percentage of live cell population. Visit www.TheTransfectionExperts.com for ideal pulse conditions.
©2011 All rights reserved. Mirus Bio LLC. All trademarks are the property of their respective owners. FLING-09/2011
Efficient Plasmid DNA Delivery into Any Cell Type Including Primary Cells
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MIR 50111 25 RXNa
MIR 50114 50 RXNa
MIR 50117 100 RXNa
a Number of electroporations per kit.
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