Knowledge is our foundation

International Scientific Advisory Board

• Steven Bustin, University of London

• Michael Pfaffl, Technical University of Münich

• Jo Vandesompele, Ghent University hospital

• Vladimir Benes, EMBL

• Gregory Shipley, University of Texas

• Preveen Ramamoorthy, Strand Life Science

30 employees across the world

Private company founded in 2001

Main investor: local Swedish

government

Among our activities

• Hands-on training courses and individual training

• Commissioned research– Send your samples to us for analysis

Among our activities

• Hands-on training courses and individual training

• Commissioned services– Send your samples to us for analysis

• R&D & product development- Focus on high performance and quality

control

• Core facility- Bring your samples and analyze them in

our core facility in Gothenburg, Sweden

TATAA training modules

1 day qPCR for miRNA analysis

1 day Sample preparation and quality control

1 day Genotyping with qPCR

1 day Immuno-qPCR

1 day Multiplex PCR

1 day Quality control of qPCR in MDx

1 day CEN/ISO guidelines for the preanalytical process in MDx

2 days Hands-on qPCR

2 days Single cell analysis

2 days Experimental design and statistical data analysis

2 days Digital PCR –Applications and analyiss

2 days NGS – Library construction and quality control

3 days Experimental design and statistical data analysis

3 days Hands-on qPCR

Quality control and quality assessment

3050 citations

www.spidia.eu

Collaboration

with BTU

New method for microRNA profiling – 3DGene

• Human

• Mouse

• Rat

• Dog

• Pig

• Monkey

• Zebrafish

As sensitive as qPCR

at lower cost.

Much higher sensitivity

than NGS

New ultrasensitive method for microRNA detection

Sensitivity approaching 25 copies!

Seeking

partners for

early access

and TACR

applications

Funded by

IMI, SSF and

Vinnova

Examples of private – public efforts

SmartHealth Next generation smart systems for cancer diagnostics

SPIDIA Standardization of the preanalytics in molecular diagnostics

MagRSA Identification of Methicillin-resistant Staphylococcus aureus

COMICS Develop sensitive indicator of DNA damage

LabOnFoil Develop ultra-low-cost laboratories on chips

CD-MEDICS Point-of-care diagnostics for the management of celiac disease

BioMatCell Growth of cells at the surface of implants

KOHERTA International multicenter trial studying circulating tumor cells

Edu-Glia Developing single cell profiling to study glia-neuron interactions

EurHealthAging/EpiTrain Epigenetic Study of Identical twins

CANCER-ID Characterization of blood based Biomarkers

Pioneered Single Cell Expression Profiling

FACS

Aspiration

Capture

FACS

Aspiration

Capture

Cellulyser

No

losses!

Freezes

profile!

Cell’s expression

changes in matter of

seconds in response to

environmental changes

FACS

Aspiration

Capture

Cellulyser GrandScript GrandMaster

PreAmp

Optimized

RT/qPCR

reagents

-When faced with ever decreasing RNA inputs, profiling

the lowly expressed transcripts of a single cell is a

constant challenge. What we needed is a reliable and

cost effective solution to provide pre-amplification of our

samples. With GrandMaster we were able to gain

consistently 2 Cq’s in our real-time qPCR experiments

that resulted in the detection of several lowly expressed

transcripts, targets that would have otherwise been lost.

Paul Collier, Research

Technician at EMBL

FACS

Aspiration

Capture

Cellulyser GrandScript GrandMaster

PreAmpHigh throughput

qPCR

BIOCEV

qPCR

facility

FACS

Aspiration

Capture

Cellulyser GrandScript GrandMaster

PreAmpHigh throughput

qPCR

GenEx

Never normalize to reference genes!

Compensate for genomic DNA

background (ValidPrime)!

Compensate for gDNA background

ValidPrime

+ gDNA specific assay (ValidPrime)

+ Reference gDNA

Original data gene 1 gene 2 gene 3 gene 4 ValidPrime

sample 1 20.1 31.1 22.1 28.2 32.5

sample 2 20.5 31.2 22.5 28.9 33.2

sample 3 21 31.1 22.9 30.2 32.3

sample 4 23.1 31.8 22.5 32.3 34.2

sample 5 23.5 30.8 22.8 32 33.1

gDNA standard 25.8 26.9 26.7 26 27

Laurell et al., Nucleic Acids Research, 2012, 1–10; Drug Discovery World (2011)

ValidPrime

gDNA

GOI

gDNA

ValidPrime

Sample

GOI

RTCqCqCqCq

GOI

RT

GOI

RTCqCqGOI

RNACq 22log2

More accurate and

more cost effective

than RT(-) controls

Measure gDNA signal in an RT- control reaction

•15% of human genes have pseudo genes

• Pseudo genes usually lack introns

• Pseudo genes are often present in multiple copies

Co-developed with

Inserm

Panels (96 markers) of

probe based RT-qPCR

assays optimized for

single cell sensitivity

Proximity

extension

Multianalyte single cell profiling

40 % 40 %

20 %

Proximity

ligation

96 proteins quantified in 1

ml of sample (body fluids)

• Oncology

• Arteriosclerosis

• Inflammatory

Collaboration opportunities with BIOCEV

• Training courses. Large discounts traditionally offered

to BTU will be expanded to whole BIOCEV. BIOCEV

experts can be hired as instructors for courses.

• Collaborations within European consortia

(Horizon2020, IMI) (biomarkers, standardization)

• Co-development of technologies with TACR funding

(microRNA analysis)

• Early access to new technologies and reagents

(mRNA expression panels; extraction kits)

• Direct/local access to highest quality qPCR/NGS

reagents at discounted pricing

Leaders work with TATAA