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5
10
15
20
25
30
35
40
Serology 0 1 1 1 0 0 1
DNA 11 3 6 12 12 20 20
Serology+DNA 0 4 9 11 12 13 15
TOTAL 11 8 16 24 24 33 36
EPT-
1
(2004
EPT-
2
(2005
EPT-
3
(2006
EPT-
4
(2007
EPT-
5
(2008
EPT-
6
(2009
EPT-
7
(2010
BEPT enrollment
44%
56%
TURKEY
BALKANCOUNTRIES
DISTRIBUTION OF PARTICIPANTS
n: 16
n: 20
SEROLOGY+DNA
DNA
SEROLOGY
TYPE OF THE REQUESTS
n: 20
n: 15
n: 1
SEROLOGY+DNA
DNA
SEROLOGY
3%
20%
11%
26%
40%
SSP
SSP+Luminex
SSP+SSO
Luminex
SSO+Luminex
METHODS IN BEPT (2010)CLASS I & II TYPING
Samples were dispatched on 7-8 December 2010
0
10
20
30
40
50
60
70
80
90
100
S#1 S#2 S#3 S#4 S#5 S#6 S#7 S#8 S#9S#10
%
BEPT-2010 CONSENSUS REPORT FOR SEROLOGICAL TYPING
***
*
100% consensus 38% consensus
0
10
20
30
40
50
60
70
80
90
100
D#1D#2D#3D#4D#5D#6 D#7D#8D#9D#10
%
BEPT-2010 CONSENSUS REPORT FOR CLASS I DNA TYPING
*
***
100% consensus 49% consensus
0
10
20
30
40
50
60
70
80
90
100
D#1D#2D#3D#4D#5D#6 D#7D#8D#9D#10
%
BEPT-2010 CONSENSUS REPORT FOR CLASS II DNA TYPING
*** ***
*
100% consensus
49% consensus
Nomenclature Mistakes
Serology 0 centerClass I DNA 13 centersClass II DNA 17 centers
Class I DNA
Class II DNA
Participant laboratories must report their findings using the correct nomenclature relevant to the methodology used.
Serology
Class I DNA
Class II DNA
Erroneous results
Serology 11 centersClass I DNA 13 centersClass II DNA 7 centers
discrepancy
Serology
Class I DNA
Class II DNA
Missing alleles
Serology 7 centersClass I DNA 1 centersClass II DNA 2 centers
Missing allele
36 LABS PARTICIPATED BEPT 2010
Centers stated to participate the tests however did not send the results
Serology A,B 1 center
Class I DNA A 0 centerB 0 centerC 5 centers
Class II DNA DR 0 centerDQ 2 centers
Problems in BEPT 2010 HLA Typing
Modification of the result submission forms (3 centers did not even use the forms we have sent)
No response about the delivery date of the samples(23 centers did not inform the date of sample delivery)
Results of 7 rounds of Region 8 BEPTfor HLA typing
has been submitted as a poster presentation to
EFI Congress to be held in Prag in May 2011.