Thermo Waters ACQUITY UPLC Systems

Thermo

Waters ACQUITY UPLC SystemsGetting Connected Guide for LC Devices

XCALI-97222 Revision F December 2013

http://www.surveymonkey.com/s/PQM6P62

© 2013 Thermo Fisher Scientific Inc. All rights reserved.

Foundation and Surveyor MSQ are trademarks, and LCQ, LCQ Fleet, LTQ, LXQ, Thermo Scientific, and Xcalibur are registered trademarks of Thermo Fisher Scientific Inc. in the United States.

The following are registered trademarks in the United States and other countries: ACQUITY, ACQUITY UPLC, nanoACQUITY, nanoACQUITY UPLC, and Waters are registered trademarks of Waters Technologies Corporation. Adobe and Reader are registered trademarks of Adobe Systems Incorporated. Microsoft and Windows are registered trademarks of Microsoft Corporation.

All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.

Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the product operation. This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.

The contents of this document are subject to change without notice. All technical information in this document is for reference purposes only. System configurations and specifications in this document supersede all previous information received by the purchaser.

This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of Sale shall govern all conflicting information between the two documents.

Release history: Rev. A, Jan 2009; Rev. B, Feb 2011; Rev. C, Dec 2011; Rev. D, May 2012; Rev. E, Dec 2012; Rev. F, Dec 2013

Software version: Thermo Foundation (see page vi for versions), Thermo LC Devices 2.2.1 or later

For Research Use Only. Not for use in diagnostic procedures.

Thermo Scientific Waters ACQUITY UPLC System Getting Connected Guide for LC Devices iii

C

Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .vRelated Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .viSystem Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .viSupported Firmware Versions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . viiCautions and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . viiContacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .viii

Chapter 1 Establishing Communication with the Data System. . . . . . . . . . . . . . . . . . . . . . . . .1Installing and Configuring a Network Interface Card . . . . . . . . . . . . . . . . . . . . . 2Connecting the Ethernet Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6Adding Waters ACQUITY to the Foundation Instrument Configuration . . . . . . 7

Chapter 2 Triggering Data Acquisition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15Connecting the Contact Closure Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15Selecting the Tray Type. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18Starting a Sequence Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Chapter 3 Troubleshooting Communications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23Verifying That the Waters Server Service is Running. . . . . . . . . . . . . . . . . . . . . 23Starting the Waters Server Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24Switching Between Direct Injection and Trapping Modes for the

nanoACQUITY System (Windows 7) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25Upgrading the Firmware for the Modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Appendix A Accessing Waters ACQUITY Help . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29Accessing Help from the Instrument Setup Window. . . . . . . . . . . . . . . . . . . . . 29

Opening Help to the First Topic . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29Opening Help for a Specific Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

Accessing Help from the ACQUITY UPLC Console Window . . . . . . . . . . . . . 31

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33

Contents

Thermo Scientific Waters ACQUITY UPLC System Getting Connected Guide for LC Devices v

P

Preface

This guide describes the following:

• How to connect a Waters™ ACQUITY™ or nanoACQUITY™ ultra-high-performance liquid chromatograph (UPLC) system to a Thermo Scientific™ mass spectrometer (MS detector) and the data system computer

• How to establish communication between the LC system and a Thermo Scientific MS data system, such as Thermo Xcalibur™.

To suggest changes to the documentation or to the Help

Complete a brief survey about this document by clicking the button below.Thank you in advance for your help.

Note Unless otherwise noted, “Waters ACQUITY” refers to both the ACQUITY and nanoACQUITY systems.

Contents

• Related Documentation

• System Requirements

• Supported Firmware Versions

• Cautions and Special Notices

• Contacting Us


Preface

vi Waters ACQUITY UPLC System Getting Connected Guide for LC Devices Thermo Scientific

Related DocumentationIn addition to this guide, the Waters ACQUITY or nanoACQUITY instrument control application provides Help. See Appendix A, “Accessing Waters ACQUITY Help.”

System RequirementsEnsure that the system meets these minimum requirements.

IMPORTANT Before you install the device driver, ensure that the data system computer has a compatible version of the Thermo Foundation™ platform as noted in the Thermo LC Devices x.x.x Release Notes.

System Minimum requirements

Computer • 2 GHz processor with 1 GB RAM • DVD drive • 80 GB available on drive C • Video card and monitor capable of 1280 × 1024 resolution • Network interface cards, two • NTFS format

Software • Adobe™ Reader™ 9.0

• Microsoft™ Windows™ operating system:

Windows 7 Professional (32-bit and 64-bita)

Windows XP Workstation SP3

• Thermo Scientific software:

aLC Devices 2.8.0 and later are compatible with Windows 7, 32-bit and 64-bit

LC Devices Foundation

2.6.0 and later 1.0.2 SP2 and later

2.5.0 SP3 1.0.2 SP2

2.5.0 SP1 or SP2 2.0

2.5.0 1.0.2 SP2

2.2.1–2.4 1.0 to 1.0.2 SP1 only

Preface

Thermo Scientific Waters ACQUITY UPLC System Getting Connected Guide for LC Devices vii

Supported Firmware VersionsLC Devices supports the model numbers and firmware versions of Waters ACQUITY and nanoACQUITY modules listed in Table 1.

Cautions and Special NoticesMake sure you follow the cautions and special notices presented in this guide. Cautions and special notices appear in boxes; those concerning safety or possible system damage also have corresponding caution symbols.

This guide uses the following types of cautions and special notices.

Table 1. Supported firmware versions for the Waters ACQUITY and nanoACQUITY LC systems

Module Firmware version

Waters ACQUITY UPLC System June 2011 Driver Pack

Binary solvent manager (BSM) 1.50.1521

Column manager (CM) 1.50.1678

Photodiode array optical detector (PDA) 1.50.1121

Sample manager (SM) 1.50.2736

Tunable ultraviolet optical detector (TUV) 1.50.2530

Waters nanoACQUITY UPLC March 2011 Driver Pack

Auxiliary solvent manger (ASM) 1.42.2537

Binary solvent manager (BSM) 1.42.1319

Sample manager (SM) 1.42.1248

Tunable ultraviolet optical detector (TUV) 1.40.2368

CAUTION Highlights hazards to humans, property, or the environment. Each CAUTION notice is accompanied by an appropriate CAUTION symbol.

IMPORTANT Highlights information necessary to prevent damage to software, loss of data, or invalid test results; or might contain information that is critical for optimal performance of the system.

Note Highlights information of general interest.

Tip Highlights helpful information that can make a task easier.

Preface

viii Waters ACQUITY UPLC System Getting Connected Guide for LC Devices Thermo Scientific

The following additional caution-specific symbol appears in the Waters ACQUITY UPLC System Getting Connected Guide for LC Devices.

Contacting UsThere are several ways to contact Thermo Fisher Scientific for the information you need.

To contact Technical Support

Find software updates and utilities to download at mssupport.thermo.com.

To contact Customer Service for ordering information

To copy manuals from the Internet

Go to mssupport.thermo.com, agree to the Terms and Conditions, and then click Customer Manuals in the left margin of the window.

To suggest changes to the Help

• Fill out a reader survey online at www.surveymonkey.com/s/PQM6P62.

• Send an e-mail message to the Technical Publications Editor at [email protected].

CAUTION Highlights electric shock-related hazards to human beings. Each electric shock notice is accompanied by the international High Voltage symbol.

Phone 800-532-4752

Fax 561-688-8736

E-mail [email protected]

Phone 800-532-4752

Fax 561-688-8731

E-mail [email protected]

Web site http://www.thermoscientific.com/en/support-landing/support.html

mailto:[email protected]

http://mssupport.thermo.com


http://www.thermoscientific.com/en/support-landing/support.html

http://mssupport.thermo.com



Thermo Scientific Waters ACQUITY UPLC System Getting Connected Guide for LC Devices 1

1

Establishing Communication with the Data System

This chapter describes how to establish communication between a Waters ACQUITY UPLC system and a Thermo Scientific mass spectrometry application, such as the Xcalibur data system.

The LC system communicates with the Thermo Scientific data system through an Ethernet connection to the data system computer. To establish this communication, follow these procedures in order:

1. Installing and Configuring a Network Interface Card

2. Connecting the Ethernet Cables

3. Adding Waters ACQUITY to the Foundation Instrument Configuration

Note Unless otherwise noted, “Waters ACQUITY” refers to both the ACQUITY and nanoACQUITY systems.

Contents

• Installing and Configuring a Network Interface Card

• Connecting the Ethernet Cables

• Adding Waters ACQUITY to the Foundation Instrument Configuration

1 Establishing Communication with the Data SystemInstalling and Configuring a Network Interface Card

2 Waters ACQUITY UPLC System Getting Connected Guide for LC Devices Thermo Scientific

Installing and Configuring a Network Interface CardThe Waters ACQUITY LC stack requires a dedicated network interface (Ethernet) card in the data system computer.

To install an additional Ethernet card in the data system computer

Follow the instructions in the manual provided by the manufacturer of the computer.

To set the IP address for the dedicated Ethernet card

1. Open the Internet Protocol Properties dialog box as follows:

a. Depending on the operating system, open the Local Area Connection Status dialog box as follows (Figure 1):

• (Windows 7 or later) From the Windows taskbar, choose Start > Control Panel > Network and Internet (or Networking and Sharing Center if set to the Icon view), and then click Local Area Connection number.

–or–

• (Windows XP) Choose Start > Settings > Control Panel > Network Connections > Local Area Connection.

CAUTION To prevent personal injury caused by an electric shock, always turn off the computer and unplug the power supply cord from the electrical outlet before removing the cover.



Figure 1. Local Area Connection Status dialog box (Windows 7 and XP)

b. Click Properties to open the Local Area Connection Properties dialog box.

Windows XP Windows 7



c. Do one of the following (Figure 2):

• (Windows 7 or later) On the Networking page, select the Internet Protocol Version 4 (TCP/IPv4) check box.

–or–

• (Windows XP) On the General page, select the Internet Protocol (TCP/IP) check box.

Figure 2. Local Area Connection Properties dialog box

Windows XP Windows 7



d. Click Properties to open the Internet Protocol Version 4 (TCP/IPv4) Properties dialog box (Figure 3).

“Version 4” and “IPv4” appear only in the dialog box for Windows 7 or later.

Figure 3. Internet Protocol Version 4 (TCP/IPv4) Properties dialog box with the IP address for the dedicated Ethernet card

2. Set the IP address as follows:

a. Select the Use the Following IP Address option.

b. In the IP Address box, type 192.168.0.1.

c. In the Subnet Mask box, type 255.255.255.0.

d. Click OK.

3. Click OK again.

Note The second network card that is dedicated to the Thermo Scientific mass spectrometer has an IP Address of 172.16.0.101 with Subnet mask 255.255.0.0.

1 Establishing Communication with the Data SystemConnecting the Ethernet Cables


Connecting the Ethernet CablesThe Waters LC system has an internal Ethernet switch located behind the column compartment. Connect each LC module in the stack to the Ethernet switch, and then connect the Ethernet switch to the data system computer with shielded Category 5 (Cat 5) Ethernet cables.

To connect the Waters LC system to the data system computer

1. Connect one end of the Ethernet cable (supplied with the LC system) to the Ethernet switch in the Waters LC stack (Figure 4).

2. Connect the other end of the cable to the dedicated Ethernet card in the data system computer (Figure 4).

For instructions on how to connect the other Ethernet cables, refer to the instrument’s documentation.

Figure 4. Ethernet connections

CAUTION Safety and EMC regulations require the use of Category 5 shielded Ethernet communication cables, maximum 3 m (10 ft) long. Use the Ethernet cables supplied in the Waters Ethernet Communication Kit.

Ethernet

Ethernet

Ethernet

Ethernet

I II

Dedicated Ethernet card

Ethernet switch at the back of the column compartment module

1 Establishing Communication with the Data SystemAdding Waters ACQUITY to the Foundation Instrument Configuration


Adding Waters ACQUITY to the Foundation Instrument Configuration To control the Waters ACQUITY LC system from the Xcalibur data system, add the LC system to the Thermo Foundation Instrument Configuration window and then set the configuration options.

To add the Waters ACQUITY system to the Foundation instrument configuration

1. Turn on the modules in the Waters LC stack.

Figure 5 shows the power switches located on the front, upper-left corner of each module.

Figure 5. Waters ACQUITY LC system (example)

Waters

Waters

WatersPower Flow Binary Solvent Manager

Sample ManagerPower Run

PDA DetectorPower Lamp

Sample OrganizerPower

Power switch

Solvent tray

PDA Detector

Column compartment

Sample Manager

Binary Solvent Manager

Sample Organizer



2. Choose Start > All Programs > Thermo Foundation x.x > Instrument Configuration to open the Thermo Foundation Instrument Configuration window (Figure 6).

Figure 6. Thermo Foundation Instrument Configuration window

3. Under Available Devices, double-click the Waters ACQUITY or Waters nanoACQUITY (not shown) icon to add it to the Configured Devices box.

4. Under Configured Devices, double-click the Waters ACQUITY or Waters nanoACQUITY icon to open the instrument configuration dialog box (Figure 7).

The dialog box shows only the General tab (page).

Figure 7. Waters ACQUITY dialog box without the module tabs



5. For the tabs (pages) to appear for each connected Waters LC module (Figure 10 on page 11), double-click one of the Plate boxes in the Plate Configuration area (Figure 7 on page 8) to open the ACQUITY UPLC Console window (Figure 8).

For the nanoACQUITY UPLC system, TUV Detector appears in the left pane instead of the PDA Detector.

Figure 8. ACQUITY UPLC Console window

Note To configure the Waters LC system, you must use the Waters ACQUITY UPLC Console (Figure 8).

System status information appears in this area.



6. Choose Configure > Configure Instrument Modules to open the Instrument Configuration dialog box (Figure 9).

Figure 9. Instrument Configuration dialog boxes for the ACQUITY and nanoACQUITY systems

7. (nanoACQUITY only) Under Fluidic Configuration, select the appropriate option for the LC configuration.

8. Under Instruments, select the check box for each LC module in the stack.

9. Click OK.

Note The fluidic configuration that you select affects the setup pages (tabs) that appear on the Xcalibur Instrument Setup window.

Note At this time, the Scan button in the Waters Instrument Configuration window is not functional.

nanoACQUITY system

ACQUITY system



10. Click Done.

11. Choose Start > All Programs > Thermo Foundation x.x > Instrument Configuration to open the Foundation Instrument Configuration window again (Figure 6 on page 8).

12. Under Configured Devices, double-click the Waters ACQUITY or Waters nanoACQUITY icon to open the instrument configuration dialog box (Figure 10).

Figure 10. Waters ACQUITY dialog box with tabs for the connected modules

13. Make sure that the dialog box contains a tab for each configured LC module.

14. Clear the Use Waters Control Panel check box.

15. Make the appropriate selections for each module, and then click OK.

Table 2 lists the General page parameters.

16. Click OK, and then click Done.

Tip Ignore the “(Not Responding)” message in the window’s title bar. The Thermo Scientific application can take several seconds to process the settings for the Waters instrument.



Table 2. General page parameters

Parameter Setting Description

Log No Log The program does not create a log of the messages it receives from the Waters LC system.

Log to File The program creates a log of the messages it receives from the Waters LC system and stores the log in the following directory:

drive:\Xcalibur\System\Programs\Waters~1.log

To keep a record of a brief operating period, create a log file. Because you cannot open a log file that exceeds 512 MB, do not use the log file to record long sequence runs.

Log to Window The Xcalibur application creates a log file that appears on the computer desktop (Figure 11).

Log to Both File and Window

See above. The same information appears in the desktop log and the Waters~1.1og file. Even when the Waters~1.1og file becomes too large to open, you can still scroll through the Waters ACQUITY for Xcalibur log (Figure 11).

Report Error to Acquisition Server Dialog boxes containing error messages appear on the computer desktop.

Reset on Initial System Errors Resets the Waters LC modules to their factory defaults after a system error occurs.

Use Waters Control Panel The Xcalibur application displays the status of the Waters LC modules in the lower portion of the Status page (Figure 12 on page 14).

When you select this check box, the options Vertical or Tabbed appear.

Note Thermo Fisher Scientific recommends that you do not use this feature (clear this check box).



Figure 11 shows an example of an Xcalibur activity log for the Waters ACQUITY system.

Figure 11. Waters ACQUITY for Xcalibur log



Figure 12 shows the Status page of the Thermo Xcalibur Roadmap view.

Figure 12. Status page of the Thermo Xcalibur Roadmap view


2

Triggering Data Acquisition

This chapter describes how to set up the LC/MS system so that the Waters ACQUITY LC system triggers the Thermo Scientific mass spectrometer to start data acquisition.

Connecting the Contact Closure CableThe Waters LC system sends a ready signal after the Waters ACQUITY Sample Manager (autosampler) makes an injection. If you want the Thermo Scientific mass spectrometer to start data acquisition after the autosampler starts an injection, you must physically connect the output terminals of the autosampler to the start terminals on the input/output (I/O) panel of the mass spectrometer.

Note To control the Waters LC system, you must use the Waters ACQUITY UPLC Console (Figure 8 on page 9).

Contents

• Connecting the Contact Closure Cable

• Selecting the Tray Type

• Starting a Sequence Run

2 Triggering Data AcquisitionConnecting the Contact Closure Cable


To connect the contact closure cable

1. Connect one end of the two-wire, contact closure cable in the Waters accessory kit to the contact closure terminal (labeled I) on the back of the Waters ACQUITY Sample Manager.

2. Connect the other end of the two-wire, contact closure cable to the START IN pins on the MS detector, following the wiring scheme listed in Table 3 and shown in the Figure 13 examples.

Table 3. Contact closure cable connections

Waters Contact closure terminal I MS detector

Pin 1 START IN +

Pin 2 START IN –

2 Triggering Data AcquisitionConnecting the Contact Closure Cable


Figure 13. Contact closure connection between the Waters ACQUITY Sample Manager and the Thermo Scientific MS

START IN

TSQ Series MS

(back panel)(right-side

panel)

+ -

LCQ FleetTM, LTQTM Series, or LXQTM MS

(right-side panel)

START IN

Back panel of the Waters ACQUITYSample Manager

RS232

RS232

Inject Start12

Out

Ethernet

Black wireRed wire

I II

START IN(5V Max)

(5V Max)

+–

LCQTM Series MS

(back panel)

(left-sidepanel)

PeripheralControl

SurveyorTM MSQTM MS

(back panel) START IN +START IN -

(Not drawn to scale.)

2 Triggering Data AcquisitionSelecting the Tray Type


Selecting the Tray Type To select the tray type

1. Choose Start > All Programs > Thermo Xcalibur > Xcalibur.

2. Click the Sequence Setup icon to open the Sequence Setup window.

3. Choose one of the following methods:

Method 1 as follows:

a. Choose Change > Tray Name to open the Tray Selection dialog box (Figure 14).

b. Click the list and select the tray type.

c. Click OK.

Figure 14. Tray Selection dialog box

–or–

Method 2 as follows:

a. Choose File > New to open the New Sequence Template dialog box (Figure 15).

b. Click the Tray Type list and select the tray type.

c. Complete the remaining template settings as applicable, and then click OK.

Click to open the list.

2 Triggering Data AcquisitionSelecting the Tray Type


Figure 15. New Sequence Template dialog box

Tip Ignore the “(Not Responding)” message in the window’s title bar. The Thermo Scientific application can take several seconds to process the settings for the Waters instrument.

2 Triggering Data AcquisitionStarting a Sequence Run


Starting a Sequence RunBy default, the Xcalibur data system selects the configured autosampler as the start instrument for a sequence run.

To ensure that the appropriate device is listed as the start instrument

1. Choose Start > All Programs > Thermo Xcalibur > Xcalibur.

2. Click the Sequence Setup icon to open the Sequence Setup window.

3. Open the sequence that you want to run as follows:

a. Choose File > Open, and then browse to the appropriate folder.

b. Select a sequence (.sld) file, and then click Open.

4. Choose Actions > Run Sequence, or Actions > Run This Sample to open the Run Sequence dialog box (Figure 16).

The “Yes” in the Start Instrument column indicates that the Waters ACQUITY instrument is the start instrument.

Figure 16. Run Sequence dialog box showing Waters ACQUITY as the start instrument

5. Under Acquisition Options, verify that the appropriate instrument is listed as the start instrument.

Selected start instrument

2 Triggering Data AcquisitionStarting a Sequence Run


6. If the appropriate instrument is not listed as the start instrument, change the start instrument as follows:

a. Click Change Instruments to open the Change Instruments In Use dialog box (Figure 17).

Figure 17. Change Instruments In Use dialog box

b. In the Start Instrument column, click the blank field to the right of the appropriate triggering device (typically an autosampler).

The word Yes moves to this field.

c. Click OK.

7. Complete the remaining selections in the Run Sequence dialog box.

8. Click OK to save the settings, close the dialog box, and start the sequence or queue it.

Tip If you want the pump or detector to turn off after a run sequence, you must manually turn off the modules.

Selected start instrument


3

Troubleshooting Communications

This chapter describes how to troubleshoot communication problems between the Waters ACQUITY LC system and the Xcalibur data system.

If there are communication problems, check the local area network (LAN) settings and the firmware versions of the Waters ACQUITY modules.

Verifying That the Waters Server Service is RunningThe Waters Server service, WDHCPServerSvc.exe, facilitates the LAN configuration for the ACQUITY modules. To establish communication between the Waters LC system and the Xcalibur data system, the Waters Server service must run as a service on the data system computer.

To verify that the Waters Server service is running

1. Ensure that the Waters LC modules are turned on and communicating with the data system computer.

2. Press CTRL+ALT+DEL to open the Windows Security window.

3. Click Task Manager.

4. Click the Processes tab.

Contents

• Verifying That the Waters Server Service is Running

• Starting the Waters Server Service

• Switching Between Direct Injection and Trapping Modes for the nanoACQUITY System (Windows 7)

• Upgrading the Firmware for the Modules

Note To verify this communication, view the system status on either the Xcalibur Roadmap view or the Waters console window.

3 Troubleshooting CommunicationsStarting the Waters Server Service


5. In the Image Name column, find WDHCPServerSvc.exe.

6. If the service is not listed, follow the procedure “Starting the Waters Server Service” on page 24.

Starting the Waters Server Service To start the Waters Server service

1. From the Windows taskbar, choose Start > Computer > drive > Program Files > Waters Instruments to open the Waters Instruments folder (Figure 18).

Figure 18. Contents of the Waters Instruments directory

2. In the Name pane, double-click the Waters DHCP Server Configuration.exe file to open the Waters DHCP Server Configuration window (Figure 19).

Select this file.

3 Troubleshooting CommunicationsSwitching Between Direct Injection and Trapping Modes for the nanoACQUITY System (Windows 7)


Figure 19. Waters DHCP Server Configuration window

3. If the modules’ IP addresses are not listed in this window, choose Server > Configuration Wizard.

The wizard configures the LAN for all modules that are turned on and connected to the data system computer.

Switching Between Direct Injection and Trapping Modes for the nanoACQUITY System (Windows 7)

Before you can switch between the direct injection and trapping modes for the nanoACQUITY system with the Windows 7 operating system, you must lower the Windows User Account Control Setting.

To switch between direct injection and trapping modes

1. From the Windows taskbar, choose Start > Control Panel > User Accounts > User Accounts (if set to Category view) > Change User Account Control Settings to open the User Account Control Settings dialog box.

2. Move the slider to the bottom position, Never Notify, and then click OK (Figure 20).

3 Troubleshooting CommunicationsSwitching Between Direct Injection and Trapping Modes for the nanoACQUITY System (Windows 7)


Figure 20. User Account Control Settings dialog box (Never Notify level)

3. Open the Waters ACQUITY UPLC Console window (see “Accessing Help from the ACQUITY UPLC Console Window” on page 31), and change the mode as applicable.

4. Reopen the User Account Control Settings dialog box, and reset the notification to the default level.

IMPORTANT Before you can use the Xcalibur data system, you must reset the user account control setting.

3 Troubleshooting CommunicationsUpgrading the Firmware for the Modules


Upgrading the Firmware for the ModulesIf the ACQUITY modules perform unexpectedly in controlling either the UPLC stack or the module communication, you might need to upgrade the device firmware to a version that is compatible with LC Devices 2.2.1 or later.

To install firmware

1. Turn on the Waters ACQUITY LC devices and ensure that they are communicating with the data system computer.

2. From the Windows taskbar, choose Start > Computer > drive > Program Files > Waters Instruments > Firmware to open the Firmware folder (Figure 21).

Figure 21. Contents of the Waters Instruments Firmware directory

3. Launch the firmware utility by double-clicking the Loader.exe file.

4. Follow the instructions that appear in the wizard.

Upgrading the device firmware can take 20–30 minutes for some of the modules.

CAUTION Because a system crash during a firmware download could cause serious harm to the Waters modules, Thermo Fisher Scientific recommends that you have a Waters service representative install firmware.

Note To verify this communication, view the system status on either the Xcalibur Roadmap view or the Waters console window.

Select this file.


A

Accessing Waters ACQUITY Help

This appendix describes how to access Help for the Waters ACQUITY instrument control software that is provided by Waters.

Accessing Help from the Instrument Setup WindowTo open Help to the first topic for the Waters ACQUITY Instrument Setup window or to the Help topic for a specific page of the view, follow these procedures:

• Opening Help to the First Topic

• Opening Help for a Specific Page

Opening Help to the First Topic

To access the Waters ACQUITY Help from the Instrument Setup window

1. Choose Start > All Programs > Thermo Xcalibur > Xcalibur, and then click the Instrument Setup icon.

2. Click the Waters ACQUITY or Waters nanoACQUITY icon to open the Instrument Setup window.

Contents

• Accessing Help from the Instrument Setup Window

• Accessing Help from the ACQUITY UPLC Console Window

A Accessing Waters ACQUITY HelpAccessing Help from the Instrument Setup Window


3. To open the Help, press the F1 key or choose Help > Waters ACQUITY Help (or Waters nanoACQUITY Help) (Figure 22).

Figure 22. Help menu for the Waters ACQUITY Instrument Setup window

Opening Help for a Specific Page

To access the Waters AQCUITY Help for a specific page of the Instrument Setup window

On the page where you need information, press the F1 key or click the Help ( ) button (Figure 23).

Figure 23. View of the Help button that opens the Help topic for the current page

Help menu

Context-sensitive Help button

A Accessing Waters ACQUITY HelpAccessing Help from the ACQUITY UPLC Console Window


For example, clicking on the General page for the Binary Solvent Manager opens the Creating a Compositional Gradient Help topic (Figure 24).

Figure 24. Creating a Compositional Gradient Help topic

Accessing Help from the ACQUITY UPLC Console Window

To access the Help from the ACQUITY UPLC Console window

1. Open the Instrument Setup window for the Waters ACQUITY device driver.

2. From the menu bar, choose Waters Acquity or Waters nanoAcquity to open the Waters ACQUITY UPLC Console window (Figure 25).

3. From the menu bar, choose Help > ACQUITY UPLC Console Help to view the Help for the selected page.

Figure 25. ACQUITY UPLC Console window

Note To configure and control the Waters LC system, you must use the Waters ACQUITY UPLC Console (Figure 25).

Help menu


I

Index

Ccables

contact closureconnecting 16connection drawings 17

Ethernet, shielded Cat 5 6configuration

adding instruments 7Waters Console 9

configure 9console

See Waters ACQUITYcontact closure

cable 16pin-out 16

contacting us viii

Ddata acquisition, triggering 15documentation

additional vionline viiisurvey viii

EEMC compliance 6errors, system reset 12Ethernet card

cables, connecting 6dedicated for LC system 2EMC compliance 6installing 2IP address, setting 2

Ethernet Communication Kit 6

Ffirmware

supported versions viiupgrades 27

GGeneral page, Waters ACQUITY dialog box 11

HHelp button 30Help, accessing 29

Iinstrument configuration, adding the LC system 7Instrument Setup window, note 10Internet Protocol (TCP/IP) Properties dialog box 5IP addresses

LC stack 5mass spectrometer 5

LLC stack, IP address 5Loader.exe 27log

See Xcalibur, log

Mmass spectrometer, IP address 5mass spectrometry application v

NnanoACQUITY

note 1TUV Detector 9


Index: P

Pparameters, General page 12power switches, LC modules 7

Rrelease notes vi

Ssequence file 20Sequence Setup, changing the start instrument 20–21start instrument, selected 20survey link viiisystem requirements vi

Ttray type, selecting 18triggering data acquisition 15troubleshooting

communication problems 23Waters Console window 9

TUV Detector 9

Uupgrading firmware 27User Account Control Settings dialog box 26

WWaters ACQUITY

(configuration) dialog box 8Console window

changing injection modes 25opening 9

firmware, upgrading 27Help, accessing 29system status, Console window 9

Waters DHCP Server Configuration window 25Waters Instruments folder 24Waters Server service

executable listing in Windows Task Manager 23starting 24

XXcalibur

important note 26log, Waters ACQUITY 13

Documents

Thermo Waters ACQUITY UPLC Systems