WAT E R S AC QU IT Y U P L C P F C A NA LYSIS K I T

Perfluorinated compounds (PFCs) are used in a wide variety of con-

sumer and industrial applications. These compounds are extremely

stable, persistent in the environment, and bio-accumulative. PFCs

such as PFOA and PFOS have been detected at parts-per-billion

(ppb) levels in wildlife tissues and human serum. As a result, there

is a need for rapid, sensitive, and accurate methods for the analysis

of PFCs in environmental and biological matrices.

The Waters® ACQUITY UPLC® PFC Analysis Kit, in combination

with the ACQUITY UPLC System, ACQUITY® TQ (tandem quadru-

pole) Detector, and MassLynx™ Software, enable environmental

testing laboratories to accurately quantitfy trace levels of PFCs by

eliminating background PFC interference. The kit includes the PFC

Isolator Column, ACQUITY BEH analytical Column, PFC Standards,

vials, and Oasis® HLB Cartridges for SPE.

Greater sample throughput for improved productivity Separation of PFCs is performed with the ACQUITY UPLC System.

UPLC® utilizes sub 2-μm pressure tolerant column particles and

high-pressure, low dispersion fluidic modules. Compared with

traditional HPLC, UPLC analyses have as much as twice the peak

resolution, are up to three times more sensitive, and can be nine

times faster – the result is greater sample throughput, lower solvent

usage, and significant reductions in cost per analysis.

High sensitivity with simplified operation The ACQUITY TQD is an easy-to-use, enhanced capability tandem

quadrupole mass detector specifically designed as an affordable,

fast MS/MS system compatible with ACQUITY UPLC. The combina-

tion of UPLC, TQD, and the PFC Analysis Kit allows for the detection

of PFOA and PFOS at sub parts-per-trillion (ppt) levels in drinking

water samples. To simplify instrument operation for users of any

experience level, the ACQUITY TQD is equipped with IntelliStart™

Software, which automatically optimizes MS parameters and moni-

tors system performance.

The solution – analogous injections are made with system modified with the ACQUITY UPLC PFC Analysis Kit. PFC contamination is delayed sufficiently to clear the analysis region and allow for accurate sample PFC quantification.

Time2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50

%

0

100

2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50%

0

100

100 pptPFOA standard

Blank injection

Time2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50

2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50

AnalytePFOA

BackgroundPFOA

Time2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00

%

0

100

2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00

%

0

100

AnalytePFOA

0.5min

Nopeaks

Time2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00

%

0

100

2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00

%

0

100

AnalytePFOA

0.5min

Time2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00

%

0

100

2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00

%

0

100

AnalytePFOA

Time2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00

2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00

AnalytePFOA

Time2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00

2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00

ACCURAT ELY QUANT IF Y PERFLUORINAT ED COMPOUNDS – EL IMINAT E BACKGROUND PFC INT ERFERENC E

The challenge of PFC contamination for quantification – an injection of 100 ppt PFOA standard and blank injection. MRM chromatograms indicate that blank contains PFOA and background PFOA peak elutes at the same retention time as analyte PFOA.

Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com

Waters, ACQUITY, ACQUITY UPLC, UPLC, and Oasis, are registered trademarks of Waters Corporation. MassLynx, TargetLynx, Xevo, IntelliStart, Quattro micro, Quattro Premier, and The Science of What’s Possible are trademarks of Waters Corporation. All other trademarks are the property of their respective owners.

©2009 Waters Corporation. Printed in the U.S.A.April 2009 720003001EN LB-PC

The PFC Analysis Kit may also be used with other Waters tandem

quadrupole mass spectrometers including the Xevo™ TQ MS, the

Quattro micro™ API, and the Quattro Premier™ XE.

Automated QA monitoring for confidence in your results Waters MassLynx and TargetLynx™ software automate data acquisi-

tion, processing, and reporting for PFC mass spectrometric data. A

range of confirmatory checks identify samples that fall outside of

user-specified or regulatory thresholds so that you can have confi-

dence in your results. With thousands of calculations and comparisons

performed automatically, your laboratory workflow will improve.

Ordering informationPRODUCT PART NUMBER

ACQUITY UPLC PFC Analysis Kit 176001744

Kit Contents

n PFC Isolator Column

n PFC standards

n ACQUITY BEH C18 Column

n Sample vials

n Oasis HLB Cartridges

n PFC isolator column assembly

n Tubing and sample loop

n Replacement solvent lines with titanium filters

n Replacement solvent bottle caps

n Solvent bottles (borosilicate glassware and caps)