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Industriell bruk og nytte av enzymer by Kjartan Sandnes , Dr. Daglig leder og forskningsleder. Biorefining salmon co- products. Enzymatic hydrolysis. Oil. Water and enzymes. Water soluble protein. Exact time and temp. Sediment. Some key figures. - PowerPoint PPT Presentation
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Industriell bruk og nytte av enzymer
by
Kjartan Sandnes, Dr.Daglig leder og forskningsleder
Biorefining salmon co-products
Sediment
Water soluble protein
Oil
Water and enzymes
Exact time and temp
Enzymatic hydrolysis
Some key figures
• Norwegian sea areas 7 times the size of land area
• All included, Norway is the 13th largest country in the world
• Second largest seafood export nation
• 53 bill. NOK • 33 mill seafood meals on
the table every day
White fish (cod, haddock, saithe) 325.000Herring 239.000Aquaculture (salmon, rainbow trout) 350.000Total co-products 914.000
«Industrial fish» (to the fish meal- and oil industry 2012) 202.000T O T A L (not for direct human consumption) 1.116.000
Norway 2012:«Non-edible fish raw materials»
NOTE! Seaweeds and shellfish not included
Source: fhf, Sildesalgslaget
Source: fhf, Sintef, Sildesalgslaget
Biomega AS –a blue biorefinery
• 2000: Marine Bioproducts AS established
• 2002: Production established with patented continuous enzymatic hydrolysis process. No chemical processing aids required
• 2007: New site acquired for new factory
• 2011: Six patent families filed so far• 2011: 30.000 tons raw materials
processed . Turnover 107 mill NOK• 2012: New factory with a capacity of
60.000 tons annually (130 mill NOK inv.) - opened May 2012
6
Constant efforts to improve quality (cooling systems) and logistics
Raw material transportation to Biomega – heads and backbones
Page 8
Raw material transportation to Biomega – viscera in bulk by truck
9
New processing plantproduction from May 2012
Overview of new plant
10
Biorefining - current and next generation products
BIOACTIVE PEPTIDES MODIFIED LIPIDS POWDERS MARINE MINERALS PEPTONES PHOSPHOSLIPIDS EXTRACTS GLUCOSAMINESFLAVOURS CHONDROITIN
BIOACTIVE PEPTIDES
N E W A N D I N N O V A T I V E P R O C E S S E Senzyme technology – extraction – separation - isolation
E X A M P L E S
Herring Hydrolysates
Salmon Hydrolysates
Test sample HH 1 HH 2MB
HR 1MB
HR 2MB
HR 3
Peptide size distribution (% of total detected)
> 30 000 daltons 0 0 2.5 0.2 0.0
30 000 – 20 000 daltons 1.3 0 0.7 0.2 0.0
20 000 – 10 000 daltons 1.2 0.2 12.6 7.1 4.6
10 000 – 5 000 daltons 12.4 5.2 17.8 15.5 10.0
5 000 - 1 000 daltons 14.6 10 13.3 9.7 10.2
1 000 - 100 daltons 53.1 66.1 38.3 40.5 33.9
< 100 daltons 17.4 18.4 14.7 26.7 41.3
Rough estimated average Mw 2100 1000 4900 2800 1900
How enzymes can be used
SEM
Typical growth medium
Nitrogen sourceAmmonium salts,Nitrates,Peptone (amino acids/peptides)
Carbon sourceGlucose,Glycerol
MicronutrientsPhosphates,Sulphates,Trace metals,Vitamins
BacteriaFungi Mammalian cellsYeasts
EnzymesAntibiotics Vaccines Other
SEM
Mask writer
Saccharomyces cerevisiae Growth Curves
0
1
2
3
4
5
6
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
Time (h)
OD
600
LACH
SOYP
OXYE
CASA
GELP
BDYE
MBPT
highest AN from supplier 1
highest AN from supplier 2
“good for yeasts” from supplier 2
Thank you!
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