Industriell bruk og nytte av enzymer

by

Kjartan Sandnes, Dr.Daglig leder og forskningsleder

Biorefining salmon co-products

Sediment

Water soluble protein

Oil

Water and enzymes

Exact time and temp

Enzymatic hydrolysis

Some key figures

• Norwegian sea areas 7 times the size of land area

• All included, Norway is the 13th largest country in the world

• Second largest seafood export nation

• 53 bill. NOK • 33 mill seafood meals on

the table every day

White fish (cod, haddock, saithe) 325.000Herring 239.000Aquaculture (salmon, rainbow trout) 350.000Total co-products 914.000

«Industrial fish» (to the fish meal- and oil industry 2012) 202.000T O T A L (not for direct human consumption) 1.116.000

Norway 2012:«Non-edible fish raw materials»

NOTE! Seaweeds and shellfish not included

Source: fhf, Sildesalgslaget

Source: fhf, Sintef, Sildesalgslaget

Biomega AS –a blue biorefinery

• 2000: Marine Bioproducts AS established

• 2002: Production established with patented continuous enzymatic hydrolysis process. No chemical processing aids required

• 2007: New site acquired for new factory

• 2011: Six patent families filed so far• 2011: 30.000 tons raw materials

processed . Turnover 107 mill NOK• 2012: New factory with a capacity of

60.000 tons annually (130 mill NOK inv.) - opened May 2012

6

Constant efforts to improve quality (cooling systems) and logistics

Raw material transportation to Biomega – heads and backbones

Page 8

Raw material transportation to Biomega – viscera in bulk by truck

9

New processing plantproduction from May 2012

Overview of new plant

10

Biorefining - current and next generation products

BIOACTIVE PEPTIDES MODIFIED LIPIDS POWDERS MARINE MINERALS PEPTONES PHOSPHOSLIPIDS EXTRACTS GLUCOSAMINESFLAVOURS CHONDROITIN

BIOACTIVE PEPTIDES

N E W A N D I N N O V A T I V E P R O C E S S E Senzyme technology – extraction – separation - isolation

E X A M P L E S

Herring Hydrolysates

Salmon Hydrolysates

Test sample HH 1 HH 2MB

HR 1MB

HR 2MB

HR 3

Peptide size distribution (% of total detected)    

> 30 000 daltons 0 0 2.5 0.2 0.0

30 000 – 20 000 daltons 1.3 0 0.7 0.2 0.0

20 000 – 10 000 daltons 1.2 0.2 12.6 7.1 4.6

10 000 – 5 000 daltons 12.4 5.2 17.8 15.5 10.0

5 000 - 1 000 daltons 14.6 10 13.3 9.7 10.2

1 000 - 100 daltons 53.1 66.1 38.3 40.5 33.9

< 100 daltons 17.4 18.4 14.7 26.7 41.3

Rough estimated average Mw 2100 1000 4900 2800 1900

           

How enzymes can be used

SEM

Typical growth medium

Nitrogen sourceAmmonium salts,Nitrates,Peptone (amino acids/peptides)

Carbon sourceGlucose,Glycerol

MicronutrientsPhosphates,Sulphates,Trace metals,Vitamins

BacteriaFungi Mammalian cellsYeasts

EnzymesAntibiotics Vaccines Other

SEM

Mask writer

Saccharomyces cerevisiae Growth Curves

0

1

2

3

4

5

6

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

Time (h)

OD

600

LACH

SOYP

OXYE

CASA

GELP

BDYE

MBPT

highest AN from supplier 1

highest AN from supplier 2

“good for yeasts” from supplier 2

Thank you!