11
臢腸腼腪膀腴膆膏臱膗臃臈 Vol. 33, pp. 413423, 2005 腙腓腜腟腘腋腐腊腎腛腢腚腞腃腖腝腔腠腗腡腁腅腄 腌腍腊腋腑腂腆腉腊腍腈腇腀腏腃腒腕 腖腣 腡腎 腅腋 1 腖腇 腙腤 腟腏 2 腉腉 腑腗 腊腘 腣腔 1 腟腝 腎腑 1 腚腢 腊腐 腅腋 1 腍腍 腠腑腊 3 腞腒 腕腋 3 腅腉 腙腤 腚腖 3 腜腓 腞腥 3 : 17 9 20 腇腎臝臬臕腛腣腈膱腤腧腦臥臶膬腖腁腦腌腅腌腐腚膈腛腓腣腖腁腦臓膐臮 臼臎腵腾腳腼腛膜膬臺腩腨腘腂臺臮腚 3 臉膲腘臥臺膬腆膠腊腦腊腗腆膽腋腧腕腂 1膰膢腖腛臓膐臮臼臎腵腾腳腼腩腂腕腲腎臡腚腺腭腵腰腹腩臷腞腦腊腗腩腦腀 腗腌腑臅腠腙臓膐臮臼臎膴臥臶膬腚腶腱腀膀腅腤臸臺臺臮腩臻腌腐腚臺臮腙腄腉腦膾膋 臤腄腣腜膾膑臁膾臁腚臧臙臤腩臭臻腌臓膐臮臼臎膪腗臯臔膪腗腖膖腌腑腚膮膐臓膐臮臼臎腙腣腦臺臮膬腛臲 6 膦臺腙膤腈臡腍腦腊腗腆腨腅腓腑腟腑6 膦臺腙腄腉腦膾膋臤腄腣腜膾膑臁膾臁腚臧臙臤腛腂腏腧腡臺臮臩腖腛膪膚腙膿腛腠腘腅腓腑腆腖臓膐臮臼臎膪腆臯臔膪腙膃腙臫膌腌腕腂腑膬腩膠腊腋腘腂臲 2 膦臺腖腛膾膋臤腙膪膚腖膄腂腛膱腤腧腘腅腓腑膸腙膰膢腖腛腺腻腳腵膀腄腣腜腫腯腳腻腮腬腀腽腚腃腩腩膺腂腲腚膬臎膳腩膡腠 腐腚膮膐腺腻腳腵膀腃腩腣腥腡腫腯腳腻腮腬腀腽腃腩腙腣腥臬腲腚膬臎膳腃腤腧 腊腧腤腅腤臓膐臮臼臎腵腾腳腼腙腄腉腦臬腲腚膬腛6 膦臺膴腖腚膾膑腚膘臝臫膌腙腣腥臡腍腑臩膴臞膚腚膾膋膨膻腙腣腥膉腇膠腊腋腧腦膍臝腆膹腃腤腧 腟腑腫腯腳腻腮腬腀腽腚腃腩腙腣腥臬腲腚臜膵膐腆腤腧腑腊腗腛腲腚腚膟臐膒腙膝腎腦腡腚腗臇腨腧腑ῐῌ῏ 臓膐臮臼臎腵腾腳腼臺臮膾自臘 臥臶臬臕腗腛臥臶腆臬膶腝膬腩膠腊 臺臮膬腢膓臨膬腩臡腍腦臌膙腖腁腥Cobb 膕腆 10膂臖腩臬臕腗臚致腌腕腂 臵腖腡70腇腎臝臬臕腗臚致腋腧腦 腐腚膈腛腒膒腋腧腕腂腘腂腇腎臝臬臕腚膈腙腔腂腕腛腊腧腟腖腙腂腈 腔腅腚膗臦腆膽腋腧腕腂腦Harrington 2腛膎 腖腚腚臖臑腅腤膅腁腀腫膈腚膛1 臢腸腼腪膀腴膆膏臱膗 膗膥臋 2 腯腷腀腲膆膗膼臀 3 臟膬膔膏膥臋 413 57

˘ ˇˆ ˙˝˛˚ ˆ˜igakukai.marianna-u.ac.jp/idaishi/www/335/08-33-5Yoshiaki... · 2006. 1. 11. · ‚ GCobb vi12 ™‚˛˙˝‚ G Cobb viwfiv37 bfivi26 #−g Fig. 3A wfivEKLbfiviFig

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  • � � ���������Vol. 33, pp. 413�423, 2005

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  • � Cristina �3������������� X���������� ��������������������� !�"#$%� Pedrini�4���&�$'�����()*+,-./��0����12�� Hadley-Miller �5����������3�4�0������ �����5�����26��� 7��� �8�� !9�#$%� Misole6��Spencer �7��� :;<"=>?@ABCD��#$EFG�%&��@� H'IJF�K���C����� L(� Willner �8��:;^ �inclina-tion� �QI���3� no 2: ��tyOP�QI^y_i� broiler, 16±�²=��� h#�� PINX �N�8�� CONT �N�8� � 2¯�'��I�c��°{�@9f.UG�^yi 4 q&

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    Fig. 2. Histology of axial sections of the vertebral body by hematoxylin-eosin staining.

    For measurement, the anterior and posterior parts were divided by the ab

    dotted line. The bar indicates 10 mm.

    A: the center of the vertebral body.

    B: the upper part of the vertebral body.

    Fig. 1. Measurement of the Cobb angle in scoliosis. In

    cases of double curves, the upper and lower

    Cobb angles were measured as a and b in the

    figure.

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    Ri 8*+ 8*�100���];� ��V���,��� 4*�50��� ���,��� 4*�50��];�� V�����G Cobb vi 12�� �����GCobbviwv 37�� bvi 26�#g� �Fig.3A� wvEKLbvi Fig. 1 � a� b��/�� l�� �Z� 14 ���tV�uV=>k]����Ri� 2 ��#i]� 8 *+ 7 *#Fig. 3B �K�� 6��-��./����6 ������.�R(� �Fig. 3B� /���� tV�uV=>k]0];�� 6���

    �� inclination �w�����{||}��� r�0.912 #c� $ 0.01 ab#1�'�{|(];� �Fig. 3C�� � 6 ����.�R];��� EKLw����� 6���� 1���{|(];��k]� � 6��#�)R2�R� 2��o�w3 Q��R¡��¢42x£";�� 2: ���� !"�#$� 2��EKL� 6���MD�I�J����J�� 2 5¤��F�� )Z�HN���CONT ^� PINX ^�#6¥��� N���`�� 2���ED�i� CONT ^� PINX ^#¦�)�I�J����J�r�)Z�H�'oiokg� �Fig. 4A�� � 6 ��#i� ¦�)I�J��)Z�H�'oiokg�� ��J�#i)7o(];� _`���K/� 6����J�#�)Z§¨)]k�og� �Fig. 4B�� 3: ���%&��'()* +�(),-!"�#$� 6��#�I�J����J��)Z�H�©

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    60

  • ������������������������ ������������ !" �Fig. 5D��#$% 2 &'��(�)*����+,-�CONT ./ PINX .'0 612'3456/7456�89�� :;

  • ���������� 4: ������������������������������������������������� !"�#$%&'�()�*+,��Cobb - 10�./� �01�� 234� ����� !'�5�67�������� !'�3�8)�*+,���6�9:;�� ?:;�� �@�A3c2BC��6���5�D1� ��E�������

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    MNJ)�O3PQ$�RSGT8U#� �;?� 3v�8qV��6� 23/�v�8VZ����ef(�� {i3|�34$� :3ef��p[(n#�� ?:;G���� (�(� {i$¥&�$(n#G#8� ¡¦8§¨�\; 2��#n7� £~3¤#�>?�©ªJ$"�� [�� *+3«Q�9:;� 6��#n$� 23*+3��(n� £�¬V3E�¡���®�£+¡3 turn over 3�§8[QV"���8¯°z;�� {i34�� 6V3 E¡�3£~3±�²����³(��´:16�3ef�:� 6��®��v�v�G��@�� 6�3£¡¬µ¶3 2\��6� 6���*+8st� 5�6/3*+$� 63

    Fig. 5. Histology of axial sections of the Th6 vertebra

    by alkaline phosphatase and acid phosphatase

    staining.

    A: Low magnified views of alkaline phosphatase

    staining in the axial sections of the Th6 vertebra.

    The bar indicates 10 mm.

    B: Low magnified views of acid phosphatase

    staining in the axial sections of the Th6 vertebra.

    The bar indicates 10 mm.

    C: High magnified views of alkaline phospha-

    tase staining in the axial sections of the Th 6

    vertebra. The bar indicates 100 mm. An arrow

    points to an osteoblast.

    D: High magnified views of acid phosphatase

    staining in the axial sections of the Th6 vertebra.

    The bar indicates 100 mm. An arrow points to an

    osteoclast.

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    62

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    ����

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    17� Cheung K. M. C and Wang T. Primary Thora-columbar Scoliosis in Pinealectomized Chick-

    ens. Spine 2003; 28: 2499�2504�18� ´µ¶� ·�¸�� >¹� -{w� >?�º»�)���¼¨©w�ª«� ¢£�

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  • � 2003; 18: 9�13�19� Roaf R. The basic anatomy of scoliosis. JBone Joint Surg 1966; 48: 786�792�

    20� Stokes I. A. F. Analysis of the interaction be-tween vertebral lateral deviation and axial rota-

    tion in scoliosis. J Biomech 1991; 24: 753�759�21� ����� ��� ���� ����� ����� ������� ������ !"#$%&'()*+,-)./�0123456789:�� ;?@� ����� ABC� D�EF� �������G#HI&JKLMN23N/O $P� ;

  • Abstract

    Morphological Study of Scoliotic Spine in Pinealectomized

    Chickens and E#ects of Melatonin and Estradiol

    Yoshiaki Torii1, Haruyasu Kato2, Ritsuko Kaneko1, Kayoko Yamashita1,

    Kazuaki Hirata1, Yutaka Sasao3, Aki Mochizuki3,

    Haruhito Aoki3, and Moroe Beppu3

    Idiopathic scoliois is the most common spinal deformity, but the etiology of the disease remains unclear.

    It has been reported that pinealectomized chicken exhibit a three dimensional spinal deformity without

    malformed vertebra. The objective of this study was to investigate the mechanism for the development of

    scoliosis in pinealectomized chickens.

    First, we identified the apical vertebra responsible for spinal deformity in pinealectomized chickens.

    Then, we measured the ossification areas and stained areas for osteoblasts and osteoclasts in the vertebral

    body and compared the pinealectomized group with the control group. As a result, we found that a wedge

    deformity of the vertebral body in the pinealectomized chickens appeared in the Th6 vertebra. Ossification

    areas and stained areas for osteoblasts and osteoclasts at that level did not di#er in the anterior part of thevertebra, but were increased in the posterior part in the pinealectomized group compared with the control

    group. In the second thoracic vertebra that was not responsible for spinal deformity, ossification areas did

    not significantly di#er between the two groups.In addition, the prevalence scoliosis were examined after melatonin and estradiol administration. As a

    result, estradiol had a more inhibitory e#ect on scoliosis compared to melatonin.Taken together, there is a possibility that scoliosis in pinealectomized chickens is attributable to an

    imbalance in ossification between the anterior and posterior parts of the Th6 vertebra, which is caused by the

    increased activity of osteoblasts and osteoclasts in the posterior part of the sixth thoracic vertebra. In

    addition, the finding of scoliosis inhibition by estradiol administration suggests the potential contribution to

    elucidation of the mechanism for scoliosis occurrence.

    1 Department of Anatomy, St. Marianna University School of Medicine2 Department of Sports medicine, St. Marianna University School of Medicine3 Department of Orthopaedic Surgey, St. Marianna University School of Medicine

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