ENZYME CLASSIFICATION EXERCISE (1) GLUCOSE + ATP  GLUCOSE-6-PHOSPHATE + ADP + H + (2) CH 3 CH 2 OH + NAD +  (CH3)CHO + NADH + H + (3) ATP + H 2 O  ADP

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    21-Dec-2015

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  • Slide 1
  • ENZYME CLASSIFICATION EXERCISE (1) GLUCOSE + ATP GLUCOSE-6-PHOSPHATE + ADP + H + (2) CH 3 CH 2 OH + NAD + (CH3)CHO + NADH + H + (3) ATP + H 2 O ADP + P i (4) CH 3 (CO)COO - + H + CH 3 CHO + CO 2 (PYRUVATE) (ACETALDEHYDE) (PYRUVATE) (ACETALDEHYDE) (5) CH 3 (CO)COO - + ATP + CO 2 ADP + P i + - OOC-CH 2 (C0) COO - (OXALOACETATE) (OXALOACETATE) - OOC H H H - OOC H H H | | | | | | | | (6) C = C C = C | | | | | | | | H COO - - OOC COO - H COO - - OOC COO -
  • Slide 2
  • ENZYME CLASSIFICATION EXERCISE (7) OXALOACETATE + GTP PHOSPHOENOLPYRUVATE + GDP + CO 2 (8) ASP + -KETOGLUTARATE OXALOACETATE + GLU (9) 3-PHOSPHOGLYCERATE 2-PHOSPHOGLYCERATE (10) - OOC-CH 2 -CH 2 -COO - + FAD - OOC-CH=CH-COO - + FADH 2
  • Slide 3
  • ENZYME CLASSIFICATION ANSWERS TO IN-CLASS EXERCISE (1) TRANSFERASE (HEXOKINASE) (2) OXIDOREDUCTASE (ALCOHOL DEHYDROGENASE) (3) HYDROLASE (ATPase) (4) LYASE (PYRUVATE DECARBOXYLASE) (5) LIGASE (PYRUVATE CARBOXYLASE) (6) ISOMERASE (MALEATE ISOMERASE) (7) HYDROLASE (PHOSPHOENOLPYRUVATE CARBOXYKINASE) (8) TRANSFERASE (AMINOTRANSFERASE) (9) PHOSPHOGLYCERATE MUTASE (10) OXIDOREDUCTASE (SUCCINATE DEHYDROGENASE)
  • Slide 4
  • OXIDOREDUCTASES CATALYZE REDOX REACTIONS SOME COMMON TYPES: REDUCTASES DEHYDROGENASES OXIDASES OXYGENASES LOOK FOR INVOLVEMENT OF COENZYMES NAD +, NADP +, FAD, FMN
  • Slide 5
  • TRANSFERASES TRANSFER OF FUNCTIONAL GROUPS SOME EXAMPLES: TRANSFERASES PHOSPHORYLASES TRANSFER OF P i OR PP i KINASES A PHOSPHATE TRANSFER UTILIZE OR GENERATE ATP
  • Slide 6
  • HYDROLASES HYDROLYSIS REACTIONS WATER MOLECULES USED TO BREAK BONDS EXAMPLES: PHOSPHATASES PEPTIDASES ATPase, GTPase
  • Slide 7
  • LYASES CATALYZES REACTIONS THAT GENERATE A DOUBLE BOND ADDS A SUBSTRATE MOLECULE TO DOUBLE BOND OF A SECOND SUBSTRATE EXAMPLES: DECARBOXYLASES DEHYDRATASES ALDOLASE
  • Slide 8
  • ISOMERASES CONVERSION OF ONE ISOMERIC FORM INTO ANOTHER EXAMPLES: ISOMERASES EPIMERASES RACEMASES MUTASES
  • Slide 9
  • LIGASES TWO MOLECULES ARE JOINED ANABOLIC REACTIONS REQUIRE NUCLEOTIDES (ATP, GTP) TO DRIVE THEM A PYROPHOSPHATE BOND MUST BE BROKEN EXAMPLES: CARBOXYLASE SYNTHETASES DONT CONFUSE SYNTHETASES WITH SYNTHASES
  • Slide 10
  • ONGOING EXERCISE USING THE PRECEEDING PRINCIPLES OF ENZYME CLASSIFICATION, MAKE SURE THAT YOU CAN IDENTIFY THE CLASS OF ENZYME FOR EACH ENZYME THAT WE STUDY AND RATIONALIZE WHY IT FITS INTO THAT PARTICULAR CLASS