8/4/2019 Programao da supra renal

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PROGRAMMING OF ADRENAL MEDULLARY FUNCTION BY NEONATAL

OVERFEEDING IN RATS1Silva AS, 1Conceio EPS, 1Oliveira E, 1Pinheiro CR, 2Trevenzoli IH, 1Cardoso FS, 1Moura EG,1Lisboa PC.

1Laboratory of Endocrine Physiology, Roberto Alcantara Gomes Biology Institute and3Laboratory of Molecular Endocrinology, Carlos Chagas Filho Biophysics Institute, Federal

University of Rio de Janeiro, RJ, Brazil.

Aim: Several studies reveal that nutritional, hormonal and environmental factors at prenatal and

neonatal period may influence organs and tissues development, and this could be related with late

diseases, like diabetes and cardiovascular disturbances (1, 2). Postnatal overfeeding increases

development risk to obesity and cardiovascular diseases. It has been shown that overfed neonate

rats show higher visceral adiposity and hyperleptinemia at weaning and they were programmed for

obesity, higher food intake and hypertension at adulthood (3). Previously, we evidenced that

neonatal hyperleptinemia induces adrenal medullary hyperfunction in early and late life (4). In the

present study, we evaluated the adrenal function of obese adult rats that were overfed duringlactation. We also evaluated indirectly the visceral adipocyte sensitivity to serum catecholamine,

through 3-adrenergic receptor (ADRB3).

Methods and results: To induce early obesity by overfeeding, the litter size was reduced from ten

to three male pups at third day of lactation until weaning (small litter group, SL) while the control

group stayed with ten males by litter over the lactation (normal litter group, NL). After weaning,

one pup from each litter (8/group) had free access to standard diet and water until the 180 days old

when they were killed and were collected samples of visceral adipose tissue (VAT), liver and the

adrenal glands. Significant differences had p