Sampling & laboratory

diagnosis

of genital infections

Types and techniques

of sampling

from the female

1- Vaginal mucus

1- Aspiration or suction method

2- Tampon method

Vaginal mucus 1 Vaginal mucus 2 Vaginal mucus 2

2- Uterine and cervical biopsy

3- Milk 1- Isolate and identify M.O (leptospira, brucella)

2- Identify antibody (brucella)

4- Blood

- Detection of circuiting antibody

- Hormonal analysis

- Serum or plasma analysis

5- Urine Isolation of M.O. (leptospira)

Types and techniques

of sampling

from the male

1- Preputial sample

2- Semen sample

3- Blood

4- Urine

Types and techniques

of sampling

from the fetus & fetal

membranes

1- Skin

2- Mouth cavity 3- Stomach content

4- Internal organ

Brucella Liver, lung

Leptospira Kidney, liver

Listeria Spleen , brain, kideny

Campylobacter lung

Mycotic abortion lung

RFV Liver, kidney

5- Whole fetus

6- Fetal membrane and fetal fluid

Bacteriological

examination

Laboratory tests

I- Cultivation and identification of MO

Brucella Farrl’s medium

Serum dextrose age medium

Blood a gar

3-10 days at 37 oC with 10-15% Co2

Campylobacter

Reed and Orr medium

Florent agar medium

Semi solid thiol medium

3-5 day at 37 oC with 10% Co2,

5% O2 and 85% N2

Trichomomas Oxoid medium

Dogl’s medium

Diamond medium

4-8 day at 37 oC

Aspergillus fumigatus

Sabouraud agar medium

3-7 day at 25oC

II- Antibiotic sensitivity test

III- Serological test

1- Rose Bengal test, card test, herd test

Idea

Sample +Ve control

Sample +Ve controlMethod

2- Serum agglutination test

Idea

Method

0.1ml serum

I ml I ml I ml I ml

1.9 ml phenol saline

1 ml phenol saline

Drop of Ag Drop of Ag Drop of Ag Drop of Ag

3- Milk ring test, Abortus bang ring test

Idea

Method

4-Mucus agglutination test (campylobacteriosis)

Homogenization of the vaginal mucus

1:4 dilution with PBS (or 5% phenol saline) using ground glass beads

Mixing the vaginal mucus 1:1 with melted oxiod agar

Incubate the solid agar with phenol saline for 18 hours at 37oC to

extract the antibody.

Solidification

2.4 ml phenol saline

0.1ml solution

0.5 ml Ag

I.25 ml I.25 ml I.25 ml I.25 ml

1.25 ml phenol saline

5- Mucus agglutination test (Trichomoniasis )

The mucus is diluted 1/5 with physiological saline and emulsified

2ml mucus

2ml melted agar

1ml mucus

2ml melted agar

1ml saline 2ml melted agar

2ml saline

1/10 1/20 -ve control

1.5 ml antigen

Tnichomonad culture diluted with 2/1 mixture of saline and 1%

glucose broth to achieve a concentration of approximately 100,000

organisms/mI

1.5 hr at 37 oC then 1.5 hr at room temperature