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Western Diagnostic Pathology Copyright - Copying is Not Permitted
Title: Revision: 2 Document Number: Page 1 of 3 Authorisation:
Specimen Collection Manual Issue 19 FRM-COLL-71 Customer Experience Manager
SPECIMEN COLLECTION MANUAL
ISSUE 19
Copy Number: Collection Centre Name:
Western Diagnostic Pathology Copyright - Copying is Not Permitted
Title: Revision: 2 Document Number: Page 2 of 3 Authorisation:
Specimen Collection Manual Issue 19 FRM-COLL-71 Customer Experience Manager
Specimen Collection Manual Table of Contents
Document Number Document Name Revision
FRM-COLL-71 Cover Page, Table of Contents and Condition of Issue 2
Guides
FRM-COLL-47 Order of Draw and Tube Guide 3
FRM-COLL-109 Specimen Bag Guide 1
FRM-COLL-110 Collection Sticker Guide 1
FRM-COLL-86 Coagulation Collection Guide 5
FRM-COLL-108 24 Hour Urine Guide 3
FRM-COLL-81 Swab Collection Guide 4
FRM-COLL-80 Urine and Genital Swab Collection Guide 5
FRM-COLL-90 Helicobacter Restricted Medication List 2
Out of Pocket (OOP) Fees
FRM-COLL-104 Out of Pocket Guide 5
Work Instructions (WI)
WI-COLL-49 Quantiferon Gold Test for TB Immunity 5
WI-COLL-83 Eye Swab - Collection Instruction 6
Collection Manual A- Z
Western Diagnostic Pathology Copyright - Copying is Not Permitted
Title: Revision: 2 Document Number: Page 3 of 3 Authorisation:
Specimen Collection Manual Issue 19 FRM-COLL-71 Customer Experience Manager
Conditions of Issue • The Specimen Collection Manual is a controlled manual under the Western Diagnostic
Pathology (WDP) Management System.
• This manual is not to be removed from its designated location. If it is moved from its
designated location, it is considered an uncontrolled manual.
• Uncontrolled manuals will not receive updates. The information in an uncontrolled manual
cannot be relied upon as current or accurate.
• Copying or partial copying of this manual is prohibited and in breach of Copyright and NATA
regulations.
• This manual is issued by WDP for use by staff, affiliated phlebotomists and clients. It always
remains the property of WDP.
• This manual is not to be marked or amended in any manner except when instructed by the
Document Controller. This includes using post-it notes for any reason except as a page
indicator.
• If you notice any incorrect or inconsistent information, please contact your supervisor or the
Customer Experience Manger (phone 08 9317 0999) as soon as possible.
• The manual is to be updated in a timely manner when a notification of change memo is
received.
• In the event of the manual being lost, damaged or destroyed the Document Controller is to
be notified as soon as possible. A further copy may be issued at the discretion of WDP.
• If this manual is found, please return it to the designated location on the front cover or the
Customer Experience Manger at Western Diagnostic Pathology, 74 McCoy St, Myaree WA
6154.
Title: Draw Order and Tube Guide Revision:3 Document Number: FRM-COLL-47 Page 1 of 1 Authorisation: Customer Experience Manager
Order of Draw & Tube Guide
Tube Tube Name Instruction Adult Paediatric
Blood Culture Refer to Blood Culture Collection (WI-COLL-74)
10mL
Streck Tube Refer to Generation NIPT Blood Collection (WI-COLL-79)
Quantiferon Gold Set Refer to Quantiferon Gold Test for TB Immunity (WI-COLL-49)
2.7mL
1.8mL
Sodium Citrate Tubes must be full draw volume
6mL
ACD
8.5mL
0.6mL
SST Let tube clot for 30 minutes prior to centrifuging
6mL
0.5mL
Plain Tube
or 6mL
2mL
0.4mL
Lithium Heparin (No Gel) PST (with gel)
Tubes must be a full draw volume
6mL
Trace Element Free
4mL
2mL
0.5mL
EDTA If ESR requested tube must be full draw volume
6mL
EDTA 6ml - Pink Tubes and declaration on request form to be signed by collector
6mL
PPT
2mL
0.6mL
Fluoride Oxalate (FLOX)
NOTE: All tubes to be gently Inverted 8-10 times. HAZARD WARNING: Never inject blood into the tube from needle and syringe. Always label tubes legibly – Surname, Given Name, Time and Date of Collection, Date of Birth and Collector’s Signature. Do not transfer blood from tube to tube to prevent contamination of additives.
Title: Specimen Bag Guide Revision:1 Document Number: FRM-COLL-109 Page 1 of 1 Authorisation: Customer Experience Manager
Specimen Bag Guide
Specimen Bag
Red
Clear
Purple
Instruction
All Urgent Samples Routine Samples Craigie IVF
Specimen Bag
Green
Blue
Orange
Instruction • Attention Specimen Reception Supervisors
• Unspun specimens • Nursing Homes/Care
facility samples
Non-Urgent Sodium Citrate Samples and other samples collected for the particular patient
Holter Monitor
Title: Collection Sticker Guide Revision:1 Document Number: FRM-COLL-110 Page 1 of 1 Authorisation: Customer Experience Manager
Collection Sticker Guide
Sticker Instruction
Specimens are deemed urgent only by the requesting doctor or Specimen Collection Manual. Please ensure that you complete all relevant fields on the sticker, write n/a if the patient is not having operation or chemotherapy. All forms must be scanned/faxed through to the urgent queue +/- phone the testing laboratory or the Laboratory Liaison Officers depending on the urgency of the test as per the Processing Urgent Specimen Protocol (PRC-ADM-1).
Where the Doctor has indicated on the request forms that the results are confidential. Only the Doctor may make this decision.
For indicating samples to be frozen on the tubes and request forms (this assist SRA that frozen sample(s) to follow). Provide the photocopy of the request form when sending frozen samples.
For indicating samples to be kept unspun due to analytical requirements.
For indicating samples to be kept unopened until testing. Test names: • Blood Alcohol • Ionised Calcium • Hep B Viral Load • Hep C Viral Load
Title: Coagulation Collection Guide Revision:5 Document Number: FRM-COLL-86 Page 1 of 2 Authorisation: Senior Scientist - Haematology
Coagulation Collection Guide
• If you calculate that you will collect more than 5 citrate tubes, have a platelet aggregation studies
or have any other queries – please ring Coagulation on 9317 0862, 9317 0861 or 9317 0863.
• Please note tests marked as $ incur out of pocket expense – the patient must be informed.
• If the transport time limit cannot be achieved, the samples can be spun and frozen as per the last column. Single or Double spinning is to be performed as per WI-COLL-40. Remember to label the tubes with ‘S/S’ or ‘D/S’ to indicate whether the sample was single or double spun.
• Frozen samples should be transported on dry ice wherever possible. For areas with shorter transit times, alternatives are acceptable so long as the samples remain frozen on receipt at the main laboratory.
Questionnaires: • Patient to complete Coagulation Questionnaire (FRM-COLL-43) for any coagulation testing. Remember
to Lab Number this form & fax it as well as the request form. • If the patient is being tested for Thrombophilia, Prothrombin gene or FV Leiden, the Genetics Tests
Questionnaire (FRM-COLL-31) should also be completed. Remember to Lab Number this form & fax it as well as the request form.
Coagulation Collections Guide Table:
Screen requested
Individually requested tests TUBES Special instructions LIMIT
(HRS) Citrate Spinning
COAGS, CLOTTING PROFILE, COAG SCREEN (includes all tests below) 2 CIT 10 Single spin
INR, PT, Prothrombin Time 1 CIT 24 Single APTT, PTT, PTTK 1 CIT 10 Single Fibrinogen, FIB 1 CIT 10 Single TCT, TT, REPTILASE 1 CIT 10 Single
D-DIMER, DD, FDP 1 CIT Always treat as urgent 4 Single spin
THROMBOPHILIA PROCOAGULANT, PROTHROMBOTIC SCREEN $
(includes all tests below)
4 CIT 1 PPT
2 EDTA 1 SST
PPT - spin within 30 min EDTA x 1 - Molecular Genetics Sticker 1 SST - is for ACL Citrates- if >10hrs, double spin/separate/freeze.
10
Double spin
ATIII (antithrombin) 2 CIT 10 Single
Protein C $ 2 CIT Patient must not be on warfarin 10 Single
Protein S $ 2 CIT Patient must not be on warfarin 10 Single
FVL (factor V Leiden) $ 2 CIT 1 EDTA
1 EDTA - Molecular Genetics Sticker 10 Single
Title: Coagulation Collection Guide Revision:5 Document Number: FRM-COLL-86 Page 2 of 2 Authorisation: Senior Scientist - Haematology
***Any combination of ALL tests – MAXIMUM 9 citrates (+/- other tube types) ***
Prothrombin Gene (PGM, G20210A, PT Gene) $ 1 EDTA 1 EDTA - Molecular Genetics
Sticker 10 Single
aPCR (activated protein C resistance) $
2 CIT 1 EDTA
1 EDTA - Molecular Genetics Sticker 10 Single
Lupus anticoagulant (LAC) 2 CIT If >10 hrs, double spin/ separate/ freeze 10 Double
Antiphospholipid Abs (LAC/ACL) 2 CIT 1 SST
1S- for ACL If more than 10 hours, double spin/ separate/ freeze Citrates.
10 Double
Homocysteine (random) 1 PPT PPT- spin within 30 minutes - N/A Anticardiolipin &/or B2GP1 Ab’s 1 SST - N/A
vWF, vWD, CLOTTING FACTORS, FACTOR STUDIES, BLEEDING SCREEN, FVIIIc, HAEMOPHILIA SCREEN (includes tests below)
3 CIT (max)
If more than 10 hours, spin/separate/freeze.
10 Single spin
FVIIIc 3 CIT 10 Single vWF:Ag 3 CIT 10 Single Ristocetin Cofactor (vWF Activity, Ricof) 3 CIT 10 Single
CBA 1 CIT 10 Single FIX, FXI, FXII 2 CIT 10 Single
PFA-100, PFA-200, PLATELET FUNCTION, BLEEDING TIME 2 CIT DO NOT SPIN stickers;
Keep at Room temp. 4 DO NOT SPIN
***Any combination of tests above – MAXIMUM 5 citrates (+/- other tube types)***
PLATELET AGGREGATION, PAG +/- mepacrine staining 5 CIT
Phone MYA Coags BEFORE collection. DO NOT SPIN stickers; Room temp. Collect before 3pm M-F To Myaree within 1 HOUR
1 DO NOT SPIN
OTHER SPECIAL TESTS 4 CIT (max) -
CAT 2 CIT 4 Double Anti-Xa, for Rivaroxaban (Xeralto), Apixaban (Eliquis) or Heparin (Clexane, Fragmin)
1 CIT Record drug name and date/ time of last dose on form. 4 Double
Anti-Platelet antibodies 2 CIT 1 SST
Citrates - DO NOT SPIN stickers; Keep at room temp; To FSH within 36hrs.
36 DO NOT SPIN
HITT Screening 4 CIT 1 PLAIN
To FSH within 4hrs or single spin/ freeze. Must notify FSH before collection. Submit vial of heparin same lot as used.
4 Single
Platelet microparticles/ Zymuphen microparticle activity 1 CIT To FSH within 4hrs or single
spin/ freeze. 4 Single
Factor Inhibitors, Inhibitor Screen, FII, FV, FVII, FX, FXIII, ADAMTS-13, XACT, PAI-1, PF1 & 2, TAT, Plasma phospholipids
1 CIT each, max 3
4 Double
Title: 24-hour Urine Guide Revision:3 Document Number: FRM-COLL-108 Page 1 of 1 Authorisation: Chief Scientist – Automated Biochemistry
24 Hour Urine Guide
TEST SAMPLE TYPE
MORE INFORMATION
5 HIAA (Hydroxy Indole Acetic Acid)
WITH ACID
Patient must be on serotonin-free diet two days prior to and during urine collection. Avoid plums, avocado, bananas, pineapple, avocado, kiwi fruit, nuts, chocolate and paracetamol.
Albumin Excretion PLAIN Overnight/timed or 24 hour collection. Note start and finish time on form.
Amylase Clearance PLAIN Calcium Excretion (CAE) WITH ACID Catecholamines/Metanephrines WITH ACID Creatinine Clearance PLAIN SST tube to be collected within 24 hours of the
urine collection e.g. when patient drops off completed sample.
Citrate WITH ACID If citrate/creatinine ratio is requested, collect a spot urine.
Urine Free Cortisol PLAIN If a dexamethasone suppression test is requested at the same time, collect this test first and schedule the dexamethasone suppression test for when the urine is returned to the collection centre.
Cystine PLAIN If cystine screen is requested, collect a spot urine.
Oxalate WITH ACID If oxalate/creatinine ratio is requested, collect a spot urine.
Always collect 24-hour urine for above tests and provide 2 container each for sample type
TEST SAMPLE TYPE
MORE INFORMATION
Bence Jones Protein/ BJP/ IEP * PLAIN
*Collect 24 hour urine only when indicated otherwise collect a spot urine with NO ACID
Calcium* WITH ACID Calcium Creatinine Ratio* PLAIN Chloride PLAIN Creatinine ACID or PLAIN Magnesium* WITH ACID Microalbumin* PLAIN Phosphate* WITH ACID Potassium* PLAIN Protein Creatinine Ratio* PLAIN Sodium* PLAIN Urea* PLAIN Uric Acid* PLAIN Urine electrophoresis* PLAIN
Title: Swab Guide Revision: 4 Document Number: FRM-COLL-81 Page 1 of 1 Authorisation: Senior Scientist – Molecular Infectious
Chief Scientist – Microbiology
Swab Collection Guide
TEST SITE DR – Doctor collect PT – Patient collect
SWAB Storage Condition: Keep at room temperate, DO NOT refrigerate
MC & S VRE Screen MRSA Screen CRE Screen
Eye Nasal Throat Wound Genital (DR/PT) Rectal (DR/PT)
Blue Top GEL Swab
MC & S
Male Urethral (DR/PT)
Orange Top GEL Swab
MRSA PCR Screen Any Red Top BD BBL Double-Headed GEL Swab
PCR / NAAT • Chlamydia Trachomatis • Neisseria Gonorrhea • Trichomonas Vaginalis • Mycoplasma Genitalium
Eye Throat Genital (DR/PT) Rectal (DR/PT) Vaginal (DR/PT)
Swab
Ⓐ
cobas PCR Media Dual Swab Sample Kit
Instruction: 1. Place swab into transport tube and snap swab at
score line 2. Discard the top of swab 3. Tightly close the lid
Endocervical (DR) Swab
Ⓑ
PCR / NAAT • Respiratory Viruses • Bordetella Pertussis • Herpes Viruses • Adenovirus • Ureaplasma • Atypical Respiratory
Any Flocked Dry Swab (NO GEL) or Flexible Dry Swab (NO GEL)
Dry Swab (NO GEL) – wire or wood
Transport Tube
FRM
-CO
LL-8
0 U
rine
& G
enita
l Sw
ab C
olle
ctio
n Gu
ide
(mc&
s and
cob
sa P
CR)
Rev:
5 Pa
ge 1
/1
Urin
e &
Gen
ital S
wab
Col
lect
ion
Gui
de
CT –
Chl
amyd
ia tr
acho
mat
is GC
– N
eiss
eria
gon
orrh
oeae
TV
– T
richo
mon
as v
agin
alis
Not
e: S
peci
men
s for
CT/
GC/T
V PC
R sh
ould
be
aliq
uote
d in
to th
e re
spec
tive
APTI
MA
tran
spor
t tu
be p
rior t
o tr
ansp
ort
SP
ECIM
ENS
REQ
UIR
ED
TE
STS
REQ
UES
TED
M
SU Y
ello
w T
op
Colle
ctio
n Po
t co
bas
FSU
Col
lect
ion
kit
BD S
traw
and
Tu
be C
olle
ctio
n De
vice
Blue
Gel
Swab
O
rang
e G
el
Swab
co
bas
un
isex
swab
kit
URINE MSU - Mid Stream FSU - First Stream
PCR/NAAT - CT/GC/TV
MC&
S ●
PCR/
NAA
T ●
●
AC
R ●
●
MC&
S +
PCR/
NAA
T ●
●
M
C&S
+ PC
R/N
AAT
+ AC
R ●
● ●
M
C&S
+ AC
R ●
●
PCR/
NAA
T +
ACR
● ●
●
GENITAL SWAB HVS/ECS/SOLVS
Urethral PCR/NAAT - CT/GC/TV
Fem
ale
MC&
S
●
Mal
e M
C&S
●
PCR/
NAA
T
● Fe
mal
e M
C&S
+ PC
R/N
AAT
●
●
Mal
e M
C&S
+ PC
R/N
AAT
● ●
FRM-COLL-90 Helicobacter Restricted Medications List Rev: 2 Page 1 of 2
EXCLUSION PERIOD TYPE OF MEDICATION
GENERIC# TRADE NAME
During Fasting and During test
Antacids ALU-TAB Andrews Tums Antacid DeWitts Antacid Powder
ENO
Gastrogel Gaviscon Mylanta Rennie Titralac
H2 Antagonists Cimetidine Magicul Tagamet Ranitidine Ausran
Ranital Rani 2 Ranoxyl
Ulcaid Ultac Zantac
Famotidine Ausfam Pamacid Pepzan
Famohexal Pepcidine
Nizatidine Nizac Tazac
Tacidine
For 7 Days Prior to Test
Proton Pump Inhibitors
Lansozaprole Lanzopran Zoton
Zopral
Rabeprazole Parzol Prabez Razit
Pariet Rabzole
Omeprazole Acimax Meprazol Omepral Pemzo
Losec Maxor Omepro-GA Ozmep
Probitor Esomeprazole Nexium Pantoprazole Gastenz
Panthron Panto Pantoloc Pantomac Somac Suvacid
Ozpan Pantofast Pantozol Salpraz Sozol Torzole
For 14 Days Prior to Test
Cytoprotectives Sucralfate Carafate Ulcyte
For 30 Days Prior to Test
Antibiotics Multiple actives Nexium Hp7 Probitor HP7
Vimovo
HELICOBACTER RESTRICTED MEDICATION LIST TRIMED PUBLICATION DATE: DEC 4 2013
FRM-COLL-90 Helicobacter Restricted Medications List Rev: 2 Page 2 of 2
For 30 Days Prior to Test . . .cont
Antibiotics Multiple actives Nexium Hp7 Probitor HP7
Amoxycillin (Penicillins)
Abbocillin Amoxil Augmentin Cilamox Fisamox Moxiclav Ranmoxy Yomax
Alphamox Bgramin Clavulin Clamoxyl Clavycillin Curam Maxamox
Ampicillin (Penicillins)
Ampicyn Ibimicyn
Austrapen
Azithromycin (Macrolides)
Azith Zedd
Zithromax Zitrocin
Clarithromycin (Macrolides)
Clarac Clarithro Klacid
Clarihexal Kalixocin
Erythromycin (Macrolides)
EES E-Mycin
Eryc Erythrocin
Roxithromycin (Macrolides)
Biaxsig Roxar
Roximycin Rulide
Tetracyclines: Akamin Doxylin Doryx Doxsig
Doxyhexal Minocycline#
Doxy Frakas Tetracycline#
Minomycin Doxycycline# Vibra-tabs
Cephalexin Keflex Cephatrust Ibilex
Cilex Ialex Rancef
Cefaclor (Cephalosporins)
Ceclor Karlor
Keflor Ozcef
Ciprofloxacin (Quinolones)
C-FloxCiprolProquin
Cifran Ciproxin
Other Anti-infectives:
Metronidazole
Tinidazole Rifaximin Bismuth
Flagyl Metronide Fasigyn Xifaxan Denol
Metrogyl Simplotan
# Apply to all generic brand of the active ingredient listed. Information compiled in this list is based on MIMS MAY/JUNE 2013. The above is NOT the exhausted list especially antibiotics and anti-infective drugs, if you unsure of the classification of the
medication, please consult your doctor or pharmacist. Note: All antibiotics except Vancomycin, Sulfa (Alprim, Bactrim, Resprim, Septrin, etc) and Amphotericin B should be
withheld for at least 4 weeks for maximal accuracy.
FRM-COLL-104 Out-of-Pocket Pricing Guide Rev: 5 Page 1 of 4
Out-of-Pocket Payments Guide Western Diagnostic Pathology (WDP) only passes on costs to patients when Medicare does not cover the cost of the test or when your tests are required to be performed by a specialised laboratory.
The prices below are to be used as a ‘guide’ only, as referring laboratories can change pricing at any time. It is the Patients responsibility to contact the referring laboratory for accurate pricing and out-of-pocket expenses.
Refer to WI-COLL-99 for detailed billing instructions • Please remember that you are required to gain consent from all patients that are having tests done
that attract an out-of-pocket fee. • To confirm consent, stamp the request form with the relevant stamp (take care not to obscure other
information on the form), complete details and ask the patient to sign to acknowledge the charge.
Test Name Out of pocket Explanation Referral lab
1, 25 Hydroxy Vitamin D $63.50 Referred test IMVS Pathology (SA)
21 Hydroxylase $34.55 Referred test Royal Prince Alfred Hosp (NSW)
24HR ABPM $100.00 Non-Medicare refundable WDP
68KD Heat Shock Protein $150.00* Referred Test ICPMR (NSW)
6TG + 6MMP $55.00 Referred Test Prince of Wales SEALS (NSW)
Activated Protein C Resistance $95.00 Non-Medicare refundable WDP
ADAMTS 13 Activity $560.00* Referred Test PathWest FSH (WA)
Aldosterone (Urine) $30.50 Referred Test Royal Prince Alfred Hosp (NSW)
Allergen Specific IgE (RAST) $40.00 for non-specialists for >4 allergens; $40.00 per recombinant allergen
Non-Medicare refundable Contact WDP Immunology (08) 9317 0920
Alpha Gal (Allergy Testing) $40.00 Non-Medicare refundable Contact WDP Immunology (08) 9317 0920
Alpha Galactosidase $178.00* Referred Test Adelaide Women’s & Children Hosp (SA)
Anabolic Steroids $440.00* Non-Medicare refundable WDP
Antimony $40.00* Referred Test Royal North Shore Hosp (NSW)
Anti-Mullerian Hormone (AMH) $75.00 Non-Medicare refundable WDP
Anti-sperm antibodies $40.00 Referred Test QML (QLD)
Antithrombin III $95.00 When Medicare criteria not met WDP
APC Resistance (Leiden) $95.00 When Medicare criteria not met WDP
Apolipoprotein E Genotype $50 .00 Referred Test Genomic Diagnostic (VIC)
ASCA $15.65 Referred Test QML (QLD)
Bismuth $30.20 Referred Test Royal North Shore Hosp (NSW)
BNP $55.00* Non-Medicare refundable WDP
BRCA 1 & 2 $399 - $1000 Non-Medicare refundable Genomic Diagnostics (VIC)
Breath Alcohol Test $5.85 Non-Medicare refundable WDP
FRM-COLL-104 Out-of-Pocket Pricing Guide Rev: 5 Page 2 of 4
Test Name Out of pocket Explanation Referral lab
Bromide $40.00* Referred Test Royal North Shore Hosp (NSW)
CA 72.4 / 74 $24.35 Referred Test Royal Prince Alfred Hosp (NSW)
Calprotectin – Faecal Crohn's Disease $85.00* Non-Medicare refundable WDP
Carbapenem Resistant Enterobacteriaceae $86.00* Non-Medicare refundable WDP
CD57 $240.00* Non-Medicare refundable WDP CDT (Carbohydrate Deficient Transferrin)
$60.00 - non-commercial clients Referred Test QML (QLD)
Chromogranin A $50.00 Referred Test QML (QLD)
Codeine (Blood) $40.00 Referred Test QML (QLD)
Cortisol (Saliva) $35.00 When Medicare criteria not met WDP
Cotinine $65.00* Referred Tests Laverty Pathology (NSW)
Cystic Fibrosis Gene Testing $190.00 Non-Medicare refundable Genomic Diagnostics (VIC) Drug Screen (Legal/Employment/Personal) $35.00 Non-Medicare refundable WDP
Drug Screen (Medicare) Nil If unsure if Medicare
funded, contact Toxicology
Contact WDP Toxicology (08) 9317 0849
ECP Assay $75.00* if over 12yo Non-Medicare refundable Royal Prince Alfred Hosp (NSW)
Elastase (Faecal) $157.50** Non-Medicare refundable PathWest FSH (WA)
Ethylbenzene Mandelic Acid $165.00 Referred Test WorkCover (NSW)
Factor V Leiden Gene Mutation $94.00* When Medicare criteria not met Genomic Diagnostics (VIC)
Faecal Calprotectin $85.00* Non-Medicare refundable WDP
Familial Hypercholesterolaemia DNA Studies
(WA) patient is covered via Medicare. NT patient need permission by Dr Sullivan due to cost of $1500. Dr
Sullivan is contactable on (02) 9515 8832. Assayed batch-wise. (Turnaround time is 60 working days - once appropriate Referral
forms received).
Referred Test PathWest FSH (WA)
FNA Cytology $95.00 No out of pocket expense if patient is Pensioner or
Veterans Affairs. WDP
Gaucher Disease Genetic Testing $2,550.00 Referred Test Adelaide Women’s & Children
Hosp (SA)
Generation Non-Invasive Prenatal Testing (NIPT)
$395.00 / $695.00 Generation Plus Non-Medicare refundable WDP
Genetic Carrier Screen $345.00* Referred Test Genomic Diagnostics (VIC)
Genetic Testing TBA Referred Test Genomic Diagnostics (VIC)
Haemochromatosis Gene (HFE) $70.00* When Medicare criteria not met Genomic Diagnostics (VIC)
HE4 $50.00* Referred Test Laverty Pathology (NSW)
Hepascore $83.90* Referred Test PathWest LMWA (WA)
Herbicides $288.00 to $750.00 (depending on type
required) Non-Medicare refundable WorkCover (NSW)
Histamine Blood $44.00 Referred Test Sullivan Nicolaides (QLD)
HIV Western Blot $15.75 + GST Test only to be added by lab WDP
FRM-COLL-104 Out-of-Pocket Pricing Guide Rev: 5 Page 3 of 4
Test Name Out of pocket Explanation Referral lab
HPV PCR (DNA)
$40.00 (for all samples not covered under the National
Cervical Screening Program)
Referred Test WDP
Hydrogen/ Methane breath test $105.00 per sugar Non-Medicare refundable Gastrolab (VIC)
Hydroxycholecalciferol Contact DM Non-Medicare refundable WDP
Iodine blood $40.00 Referred Test Royal North Shore Hosp (NSW)
Iodine urine $159.00* Referred Test Laverty Pathology (NSW)
Isocyanates urine $300.00 Referred Test WorkCover (NSW)
JAK 2 Mutation $150.00* Non-Medicare refundable Laverty Pathology (NSW)
KIT Gene for Mastocytosis $99.00 Non-Medicare refundable Peter McCallum Cancer Centre (VIC)
LDL Subfractions $170.00 Non-Medicare refundable San Pathology – Sydney Adventist Hospital (NSW)
Leflunomide $34.80* Referred Test Royal Brisbane Hosp (QLD)
LEPTIN $30.70* Referred Test Royal Prince Alfred Hosp (NSW)
Mantoux test - Human $90.00 Non-Medicare refundable WDP
Mesothelin $260.00* Referred Test QML (QLD)
MTHFR gene test $70.00 Referred Test Genomic Diagnostics (VIC)
Naltrexone $250.00* if qualitative $500.00* if quantitative
Referred Test Forensic Science Laboratory Chemistry Centre (WA)
NT-proBNP $55.00* Non-Medicare refundable WDP
Osteocalcin $75.00 Referred Test WDP
Ovulation tracking $100.00 per cycle Non-Medicare refundable WDP
Paternity testing TBA Non-Medicare refundable Genomic Diagnostics (VIC) Patient to call for pricing -
1800 822 999 Pesticides $254.00 Commercial Clients WorkCover (NSW)
PGx MH $147.00 Non-Medicare refundable Genomic Diagnostics (VIC)
PGx Multi $197.00 Non-Medicare refundable Genomic Diagnostics (VIC)
PGx Pain $147.00 Non-Medicare refundable Genomic Diagnostics (VIC) Placenta-like growth factor (PLGF) $55.00 Non-Medicare refundable WDP
Pompes disease $265.00 Referred Test Adelaide Women’s & Children Hosp (SA)
Procollagen Type 3 NT $175.00 Referred Test Sullivan Nicolaides Pathology (QLD)
Prostate health index (PHI) $90.00* Non-Medicare refundable QML (QLD)
Protein C assay $95.00 Non-Medicare refundable WDP
Protein S assay $95.00 Non-Medicare refundable WDP
Prothrombin G20210A mutation $94.00* When Medicare criteria not met Genomic Diagnostics (VIC)
PSA
Medicare will pay a rebate for only one PSA test
performed in any 12-month period in the absence of
previously abnormal results or previously diagnosed
prostatic disease. A non-refundable fee of $50.00* will be charged if not billed
through Medicare
Non-Medicare refundable WDP
PTH related peptide (PTHRP) $100.00 Referred Test WDP
Pyrrole Urine Test $80.00 Referred test Safe Analytica Labs (QLD)
FRM-COLL-104 Out-of-Pocket Pricing Guide Rev: 5 Page 4 of 4
Test Name Out of pocket Explanation Referral lab QF-PCR - Amniocentesis, CVS, other $120.00 Referred Test WDP
Quantiferon TB gold assay
$70* will be charged unless
immunocompromised. Commercial Clients are charged according to
contracted rates.
Non-Medicare refundable
WDP
RAST
$40.00 for non-specialists for >4 allergens; $40.00
per recombinant allergen. $40.00 per request for
AraH2, Alpha-Gal (Mammalian Meat Allergy),
α-Lactalbumin, β-Lactoglbulin, Casein or
Omega05-Gliadin
Non-Medicare refundable Contact WDP Immunology (08) 9317 0920
Reverse T3 $100.00* Referred Test QML (QLD)
Salbutamol $25.00 Referred Test QML (QLD)
Salivary Hormones
$25.00 per hormone for the first 5 hormones + $30.00 handling fee. $15.00 for
additional hormones
Referred Test Healthscorp Pathology (Incorp APL)
Soluble transferrin receptor $20.30 Non-Medicare refundable Fiona Stanley Hosp (WA)
Specific IgE (RAST) $40.00 for non-specialists for >4 allergens; $40.00 per recombinant allergen
Non-Medicare refundable Contact WDP Immunology (08) 9317 0920
Spinal muscular atrophy $195.00* Non-Medicare refundable Genomic Diagnostics (VIC)
Synthetic cannabinoids $50.00 Non-Medicare refundable WDP
Syphilis confirmation $22.00 Test only to be added by lab WDP
T Cell/T Cell Receptor Gene Rearrangements $70.00 Non-Medicare refundable QML (QLD)
Thrombophilia screen $95.00 unless a pensioner or DVA or relevant clinical
history Non-Medicare refundable WDP
Titanium $30.80 Referred Test Royal North Shore Hosp (NSW)
Vancomycin resistant Enterococci $55.00 Non-Medicare refundable WDP
Very long chain fatty acids $217.00* Referred Test Adelaide Women’s & Children Hosp (SA)
White cell enzymes $462.00 Referred Test WDP *Denotes prices updated 17.01.2019 **Denotes prices updated 26.03.2019
WI-COLL-49 Quantiferon Gold Test for TB Immunity Revision: 5 Page 1 of 3
RELEVANT OSH & OTHER LEGISLATION, STANDARDS, GUIDELINES: Occupational Safety and Health Act 1984 Occupational Safety and Health Regulations 1996 Work Health and Safety (National Uniform Legislation) Act 2011 Work Health and safety (National Uniform Legislation) Regulations
Purpose To determine TB immunity Scope
• Designated WDP ACC locations • Medicare is only rebateable for immunosuppressed or immunocompromised patients • Quantiferon-TB Gold Plus Data Sheet Patient Questionnaire (FRM-COLL-33) to be completed for ALL
requests (including commercial, pre-placement & Occupational Health) • Commercial testing is Non-Medicare rebateable for Occupational Health, Insurance, Travel, Visa,
Employment purposes eg Medical and Nursing students, etc. An Account will be issued.
When requested on a Medicare form, the patient must be informed that Medicare does not fully cover the cost of this test, and an out of pocket charge will apply. Give the patient a brochure explaining the test and an Out Patient Billing Guide brochure.
Materials: Quantiferon-TB Gold Plus® Tubes (1 set per patient)
• Grey Cap = NIL – negative control tube • Green Cap = TB1 - antigen tube • Yellow Cap = TB2 - antigen tube • Purple Cap = Mitogen – positive control tube
WORK INSTRUCTION Quantiferon Gold Test for TB Immunity
DEPARTMENT Collections
DOCUMENT No WI-COLL-49
AUTHORISATION Customer Experience Manager
Identified Work Health and Safety Risk Personal Protective Equipment Required
Required Additional Control Measures:
Biological hazard Gloves - Nitrile Gowns Goggles Masks Closed in shoes
Immunisation Needlestick/ biohazard pack Training Spill kits Spills procedure 1st aid kit Biohazard / clinical waste bin
Needle stick/sharps exposure Gloves - Nitrile Gowns Goggles Masks Closed in shoes Aprons
Immunisation Needlestick/ biohazard pack Biohazard / clinical waste bin Training Spill kits Spills procedure 1st aid kit Vacutainers Sharps containers No recapping
WI-COLL-49 Quantiferon Gold Test for TB Immunity Revision: 5 Page 2 of 3
Order of Draw: Blood culture and citrate tubes are the only tube that can precede Quantiferon Gold ® Tubes in the order of draw Quantiferon tube order is Grey, Green, Yellow and Purple (GGYP) Procedure:
4.a.1 Using standard venepuncture technique, collect 1mL into the each of the four (4) Quantiferon-TB Gold Plus® tubes (Each of the tubes MUST be filled within the 1.0 mL black fill range line marked near the bottom of the tube). If blood is not filled appropriately in all 4 tubes, please recollect while patient is still available. Inappropriately filled tubes will NOT be accepted by the laboratory and a recollect will be issued. If using a butterfly needle, prime tubing with a “purge” tube (not supplied)
Figure 1. Example Correctly Filled Quantiferon Tube (for ALL 4 tubes).
b. Collect Quantiferon Gold ® Tubes in strict order of: i. Grey Cap
ii. Green Cap iii. Yellow Cap iv. Purple Cap
d. Quantiferon Gold ® Tubes have a slow draw/fill. Keep the tube on the needle until blood flow has ceased, to ensure correct volume has been drawn/filled.
e. Immediately after filling the tubes, gently invert 10 times, to ensure that the entire inner surface of the tube is coated with blood, to dissolve antigens on tube walls.
NOTE: Excessive shaking may cause incorrect results.
f. Label tubes as per WI-COLL-8 and forward to the laboratory in a specimen bag within 16 hours of collection.
g. DO NOT refrigerate samples.
Billing Information • Commercial forms will have a clearly identified Account Code to whom the account will be sent
• Immediate payment forms will clearly specify the value of the immediate payment required from the patient at the time of collection.
• Pathology Request forms will typically have a Medicare Assignment section
ALL 4 Quantiferon-TB Gold Plus tubes MUST be filled within this black line. Blood must not be above or below this line.
WI-COLL-49 Quantiferon Gold Test for TB Immunity Revision: 5 Page 3 of 3
FAQs
• ONLY Quantiferon-TB Gold Plus® Tubes can be used for this testing • Uncollected tubes are stored at room temperature. • Expiry date must be checked BEFORE specimen is collected - DO NOT use tubes past the stated expiry
date that is printed on the tube • If a “butterfly needle“ is used to collect the samples, a “purge“ tube must be used to ensure tubing is
filled with blood prior to the filling of the Quantiferon-TB Gold Plus® Tubes.
Title: Eye Swab Collection Revision: 6 Document Number: WI-COLL-83 Page 1 of 2 Authorisation: Customer Experience Manager
RELEVANT OSH & OTHER LEGISLATION, STANDARDS, GUIDELINES: Occupational Safety and Health Act 1984 Occupational Safety and Health Regulations 1996 Work Health and Safety (National Uniform Legislation) Act 2011 Work Health and safety (National Uniform Legislation) Regulations
Eye Swab
Conjunctivitis is an irritation or inflammation of the conjunctiva, which covers the white part of the eyeball. It can be caused by allergies or a bacterial, viral or chlamydial infection. Conjunctivitis can be extremely contagious and is spread by contact with eye secretions from someone who is infected.
If the patient is taking antibiotics, record the name of the medication on the request form.
Procedure
1. Review the request form and select the appropriate swab and media. • MC&S – Blue top gel swab • Viral PCR – Orange top dry swab • Chlamydia – cobas PCR swab
2. a) Sit the patient with the head well supported. Tilt the patients head back, ask the patient to look upwards to protect the cornea and gently pull down the lower lid exposing the conjunctiva.
b) Very Gently guide the swab tip along the inside of the bottom eye lid from the inner corner to the outer corner of the eye. Take care not to touch the eyelids.
WORK INSTRUCTION Eye Swab – Collection Instructions
DEPARTMENT Collections
DOCUMENT No WI-COLL-83
AUTHORISATION Customer Experience Manager
Identified Health and Safety Risk
Personal Protective Equipment Required:
Required Additional Control Measures:
Biological hazard Gloves - Nitrile Closed in shoes
Immunisation Needlestick/ biohazard pack Training Biohazard / clinical waste bin
Title: Eye Swab Collection Revision: 6 Document Number: WI-COLL-83 Page 2 of 2 Authorisation: Customer Experience Manager
c) Place the swab immediately into the medium container, capping tightly and label with Patient surname, first name, DOB, time and date of collection.
d) Ask the patient to close the eye for a few seconds, this will ensure the safe technique of swab taking and avoid damage to the cornea.
e) If bilateral swabs are requested, a separate swab is required for each eye and gloves must be changed between each swab to prevent cross contamination between eyes.
NOTE – OBTAINING SWABS FROM CHILDREN (excluding neonates) When it is necessary to take a swab from a small child/baby it is important that the procedure is fully explained to the parents. Let them assist by holding the child on their lap and supporting their head. This will help to minimise fears and anxieties. They will need constant reassurance as the child usually cries a lot and parents often find this distressing. If the collector encounters difficulties where the swab cannot be safely obtained, then the child should be referred to the requesting doctor for a clinician collect.
Test Name Out of Pocket Specimen Collection Method Min
Vol
1, 25 HYDROXY VITAMIN D Yes 1 x Plain Tube (Red Top) - Spin, Separate & Freeze
Referred to: SA Pathology (Formally known as IMVS Pathology)
1.5mL
17 OH CORTICOSTERONE 1 x Urine Container - random
Referred to: Dorevitch Pathology
17 OHP (17- HYDOROXY PROGESTERONE)
1 x Plain Tube (Red Top) - Spin & Separate
Referred to: PathWest FSH Can be frozen if androstenedione co-requested. If a dexamethasone suppression test is requested at the same time, collect this test on day one and schedule the dexamethasone suppression test for day 2. Call the Duty Biochemist if recollection is required.
1mL
21 HYDROXYLASE Yes 1 x SST spin separate and freeze
Referred to: Royal Prince Alfred Hospital
0.5mL
25 HYDROXY VITAMIN D (25 OHD) Yes 1 x SST Western Diagnostic Pathology will bulk bill when indicated by referring doctor. WDP assume requesting doctor has determined that patient meets requirements as stipulated in the Medicare Benefits Schedule (Item No: 66833) **Request form to be stamped with "Patient Advised of Fee" and signed by the patient acknowledging that an out of pocket payment will be due. See: WI-COLL- 113 Vitamin D Bulk Billing Criteria
2mL
25 HYDROXYCHOLECALCIFEROL Yes 1 x SST Western Diagnostic Pathology will bulk bill when indicated by referring doctor. WDP assume requesting doctor has determined that patient meets requirements as stipulated in the Medicare Benefits Schedule (Item No: 66833) Request form to be stamped with "Patient Advised of Fee" and signed by the patient acknowledging that an out of pocket payment will be due. See: WI-COLL- 113 Vitamin D Bulk Billing Criteria
2mL
5 - FLUOROCYSTOSINE 1 x SST Referred to: PathWest QEII 2mL
5 - FLUOROURACIL 1 x SST Referred to: PathWest QEII
Specimen Collection Manual Issue 19 Page 1 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
5 HIAA 1 x Urine Container- 24hr acid
Referred to: PathWest FSH A random urine can be collected for children. Patient must be on serotonin-free diet two days prior to and during urine collection. Avoid plums, alcohol, bananas, pineapple, avocado, kiwi fruit, nuts, chocolate and paracetamol.
10mL
50G GESTATIONAL DIABETES SCREEN
1 x Fluoride Oxalate
Patient does not need to be fasting. 1. Give 50g glucose drink2. 1-hour post drink, take 1x FLOX
2mL
68KD HEAT SHOCK PROTEIN Yes 1 x SST Referred to: ICPMR 4mL
6TG + 6MMP Yes 1 x EDTA - dedicated tube required
Referred to: Prince of Wales - SEALS 4mL
ACANTHOCYTES (URGENT – Result within 4 hours)
1 x EDTA The EDTA must be at the lab within 3 hours. If in doubt, please ring Haematology Dept. on 08 9317 0861.
4mL
ACE 1 x SST Referred to: QML Pathology 1mL
ACE - CSF DOCTOR COLLECT Referred to: PathWest QEII 0.3mL
ACETYLCHOLINE RECEPTOR AB 1 x SST Referred to: QML Pathology 1mL
ACETYLCHOLINESTERASE GENOTYPING
1 x EDTA Referred to: PathWest QEII 1mL
ACID PHOSPHATASE 1 x SST Referred to: AWCH 2mL
ACR 1 x Urine Container - random
Transfer into Vacutainer (no additive) urine tube. Urine tubes must not be filled beyond half way to prevent spillage.
2mL
ACTH 2 x Chilled EDTA - Spin, separate and freeze
2 x EDTA must be chilled for at least 15 minutes prior to the collection. Then, spin, separate and freeze ASAP. If CORTISOL is co-requested ensure the both 2 x chilled EDTA and 1x SST samples are collected at the same time.
2mL each
ACTIVATED PARTIAL THROMBOPLASTIN TIME
2 x Sodium Citrate
NOT for patients on Heparin. To lab within 10hrs - transport in blue bag. Or single spin/separate/freeze and transport frozen. Patient to complete questionnaire: FRM-COLL-43 See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
Specimen Collection Manual Issue 19 Page 2 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
ACTIVATED PARTIAL THROMBOPLASTIN TIME (HEPARIN MONITORING)
1 x Sodium Citrate For patients on Heparin only. Record date and time of last dose. Must be at the lab within 4hrs - transport in blue bag. Or single spin/separate/freeze and transport frozen. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
ACTIVATED PROTEIN C RESISTANCE
Yes 2 x Sodium Citrate NOTE: out of pocket expense unless a pensioner, DVA or relevant personal/family history. To Myaree within 10hrs - transport in blue bag. Or single spin/separate/freeze and transport frozen. Patient to complete questionnaire: FRM-COLL-43. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
ACTIVE B12 1 x SST 2mL
ACYLCARNITINES 1 x Lithium Heparin spin, separate & freeze ASAP
Referred to: PathWest QEII
ADAMTS13 Yes 2 x Sodium Citrate Referred to: PathWest FSH Must be at the lab within 4 hrs - transport in blue bag. Or DOUBLE spin/separate/freeze and transport frozen. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
ADDISON'S SCREEN 2 x Chilled EDTA - Spin, separate and freeze and 1 x SST
2mL
ADENOVIRUS SEROLOGY 1 x SST Referred to: QML Pathology 1mL
ADRENAL ANTIBODIES 1 x SST Referred to: PathWest QEII 1mL
Specimen Collection Manual Issue 19 Page 3 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
ADRENOCORTICOTROPHIC HORMONE (ACTH)
2 x Chilled EDTA - Spin, separate and freeze
Chill 2 x EDTA tubes prior to collection. Spin, separate and freeze within 30 minutes of collection at ACC. If a CORTISOL is co-requested, then collect 1 X SST and ensure all samples are collected at the same time. If recollect is required for ACTH ensure SST is also collected at the same time for cortisol.
2mL each
AFP 1 x SST 2mL
AFP (AMNIOTIC) DOCTOR COLLECT 2mL
AH50 1 x SST - Spin and freeze
Referred to: PathWest QEII Must be frozen within 1 hour of collection into 2 separate 1mL aliquots.
1mL each
ALBUMIN 1 x SST 2mL
ALBUMIN (TIMED URINE EXCRETION)
Note on the form the start date and time and the end date and time of the urine collection. Transfer into Vacutainer (no additive) urine tube. Urine tubes must not be filled beyond half way to prevent spillage.
2mL
ALBUMIN (FLUID) DOCTOR COLLECT 2mL
ALBUMIN/CREATININE RATIO 1 x Urine Container - random
2mL
ALCOHOL (BLOOD) 1 x Fluoride Oxalate - DO NOT OPEN
Must be a pristine Fluoride Oxalate/SST tube – place DO NOT OPEN sticker around the lid. Alcohol swab must not be used to clean area. Police/legal purpose sample Western Diagnostic Pathology is not licensed to perform Blood Alcohol analysis for police or legal purposes. Such samples (usually in a sealed envelope) cannot be accepted. Do not accept the samples and advise the patient to contact the Forensic Science at ChemCentre on 08 9422 9800 for cost and instruction.
2mL
Specimen Collection Manual Issue 19 Page 4 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
ALDOSTERONE/ RENIN (ARR) 1 x PPT - spin and freeze
If Erect (upright) is requested, ensure that the patient has been walking or sitting for at least 30 min prior to collection. If Supine (lying down) is requested, ensure that the patient have been lying down for at least 30 min prior to collection. If posture have not been specified on the form, please collect erect sample. Please note time and posture clearly on the form. Aldosterone and Renin are usually co-requested but can be requested individually. Spin samples within 30 min of collection and DO NOT REFRIGERATE
3mL
ALEXANDERS DISEASE 1 x EDTA - dedicated tube required
Referred to: PathWest FSH 2mL
ALKALINE PHOSPHATASE 1 x SST 2mL
ALKALINE PHOSPHATASE ISOENZYMES
1 x SST 1mL
ALPHA 1 ANTITRYPSIN GENOTYPE 1 x SST - dedicated tube required and 1 x EDTA - dedicated tube required
Referred to: PathWest QEII 1mL each
ALPHA 1 ANTITRYPSIN LEVEL 1 x SST Referred to: QML Pathology 2mL
ALPHA 2 GLOBULIN 1 x SST 2mL
ALPHA FETOPROTEIN 1 x SST 2mL
ALPHA GAL (RAST/SPECIFIC IGE) Yes 1 x SST NOTE: This is not ALPHA GALACTOSIDASE
1mL
ALPHA SUBUNIT 1 x SST spin separate and freeze
Referred to: PathWest QEII 1mL
ALPHA SUBUNITS OF GLYCOPROTEIN HORMONES
1 x SST - Spin and freeze
Referred to: St Vincent's Pathology 2mL
ALPHA-1-ANTITRYPSIN (FAECES) Yes 1 x Faeces Container - Freeze, dedicated jar
Referred to: Royal Brisbane Hospital If a FAECAL CALPROTECTIN is co-requested, 2 separate samples to be collected.
5g
ALT 1 x SST 2mL
Specimen Collection Manual Issue 19 Page 5 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
ALUMINIUM BLOOD Yes 1 x Trace Element Free - spin & separate
Referred to: Laverty Pathology. 2mL
ALUMINIUM URINE 1 x Urine Container - random
Referred to: Laverty Pathology 10mL
AMIKACIN 1 x Lithium Heparin spin, separate & freeze ASAP
Referred to: PathWest QEII 1mL
AMINO ACID SCREEN PLASMA 1 x Lithium Heparin spin, separate & freeze ASAP
Referred to: PathWest QEII 1mL
AMINO ACID SCREEN URINE 1 x Urine Container - dedicated jar required. Freeze ASAP
5mL
AMIODARONE 1 x Plain Tube (Red Top)
Referred to: QML Pathology Provide clinical and medication details. Collect immediately prior to next dose. Record time and date of last dose.
1mL
AMITRIPTYLINE 1 x Plain Tube (Red Top)
Referred to: QML Pathology Collect trough level. Record time and date of last dose.
2mL
AMMONIA 1 x Lithium Heparin
Sample collected at Joondalup collection centre: Place immediately on ice, centrifuge the sample as soon as possible, separate plasma, and store at 2-4°C until analysis. Samples collected at other than Joondalup collection centre: Place immediately on ice, centrifuge the sample as soon as possible, separate plasma, freeze, and send to the testing laboratory on dry ice.
5mL
AMOXYCILLIN Test Not Available
AMYLASE 1 x SST 2mL
AMYLASE (FLUID) DOCTOR COLLECT 2mL
AMYLASE (URINE) 1 x Urine Container - random
Transfer into Vacutainer (no additive) urine tube. Urine tubes must not be filled beyond half way to prevent spillage.
2mL
Specimen Collection Manual Issue 19 Page 6 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
ANAFRANIL 1 x Plain Tube (Red Top)
Referred to: PathWest QEII 2mL
ANCA 1 x SST 1ml
ANDROGEN STUDIES 1 x SST 2mL
ANDROSTENEDIONE 1 x Plain Tube (Red Top) - Spin, Separate & Freeze
Referred to: PathWest FSH 0.5mL
ANF 1 x SST 1mL
ANION GAP 1 x SST Must be co-requested with electrolytes.
2mL
ANTENATAL SCREEN 1 x EDTA 6mL - Pink, dedicated tube
1. Full Name, DOB or URN, DOC & TOC must be on EDTA tube and request form. All information must match between form and sample.
2. Collector must sign the tube and certifier on form - these signatures must match.
Contact Transfusion Medicine Dept. on 08 9321 3300 for any enquiries.
6mL
ANTI DIURETIC HORMONE (ADH) 1 x SST and 1 x Urine Container - random
2mL each
ANTI GAD 1 x SST Referred to: QML Pathology 2mL
ANTI IGA ANTIBODIES 1 x SST Referred to: PathWest QEII 5mL
ANTI ISLET CELL ANTIBODY 1 x SST Referred to: PathWest QEII 2mL
ANTI LC1 ANTIBODIES 1 x SST Referred to: PathWest QEII 2mL
ANTI MULLERIAN HORMONE (AMH)
Yes 1 x SST 1mL
ANTI NEURONAL ANTIBODY 1 x SST Referred to: PathWest QEII 2mL
ANTI NMDA RECEPTOR ANTIBODIES
1 x SST Referred to: PathWest QEII 2ml
ANTI PLATELET ANTIBODIES (CALL BEFORE COLLECTION)
2 x Sodium Citrate - DO NOT SPIN and 1 x SST - dedicated tube required
Referred to: PathWest FSH Only collect on Monday to Thursday. Do not collect on Public Holidays. PathWest will not test after 2pm on Fridays. Citrate tubes to have 'DO NOT SPIN' stickers attached. To be at PathWest FSH within 36hours of collection. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL each
Specimen Collection Manual Issue 19 Page 7 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
ANTI SKELETAL MUSCLE ANTIBODY
1 x SST Referred to: PathWest QEII 2mL
ANTI SKIN ANTIBODIES 1 x SST Referred to: PathWest QEII 2mL
ANTI SOLUBLE LIVER ANTIGEN 1 x SST Referred to: PathWest QEII 2mL
ANTI SPERM ANTIBODIES Yes 1 x SST Referred to: QML Pathology Semen sample is acceptable too.
5mL
ANTI THYROGLOBULIN ANTIBODIES (ATG)
1 x SST 1mL
ANTI THYROID ANTIBODY (TPO) 1 x SST 1mL
ANTI VGKC ANTIBODY 1 x SST Referred to: PathWest QEII 5mL
ANTIBODY SCREEN 1 x EDTA 6mL - Pink, dedicated tube
1. Full Name, DOB or URN, DOC & TOC must be on EDTA tube and request form. All information must match between form and sample.
2. Collector must sign the tube and certifier on form - these signatures must match.
Contact Transfusion Medicine Dept. on 08 9321 3300 for any enquiries.
6mL
ANTIBODY TITRE 1 x EDTA 6mL - Pink, dedicated tube
1. Full Name, DOB or URN, DOC & TOC must be on EDTA tube and request form. All information must match between form and sample.
2. Collector must sign the tube and certifier on form - these signatures must match.
Contact Transfusion Medicine Dept. on 08 9321 3300 for any enquiries.
6mL
ANTI-CCP 1 x SST 2mL
ANTI-CHROMATIN ANTIBODIES 1 x SST Referred to: Laverty Pathology Only if ANA is positive.
1mL
ANTI-DNASEB 1 x SST Referred to: QML Pathology
2mL
ANTI-MAG (MYELIN ASSOCIATED GLYCOPROTEIN)
1 x SST Referred to: PathWest FSH 1mL
ANTIMONY Yes 1 x Trace Element Free Tube
Referred to: Royal North Shore Hospital
5mL
Specimen Collection Manual Issue 19 Page 8 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
ANTINUCLEAR ANTIBODY (ANA) 1 x SST 1mL
ANTI-NUCLEOSOME ANTIBODIES 1 x SST Referred to: Laverty Pathology Only if ANA is positive.
1mL
ANTIPHOSPHOLIPID ANTIBODIES 2 x Sodium Citrate and 1 x SST
Citrate to Lab within 10 hours - transport in blue bag. OR DOUBLE spin/separate/freeze and transport frozen. Patient to complete questionnaire FRM-COLL-43 Anti phospholipid consists of three tests: Lupus anticoagulant, anticardiolipin and beta 2 glycoprotein. See: FRM- COLL-86 - Coagulation Collection Guide
2.7mL each
ANTITHROMBIN III Yes 2 x Sodium Citrate To Myaree within 10 hours - transport in blur bag. Or single spin/separate/freeze and transport frozen. Patient to complete Questionnaire: FRM-COLL-43. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
ANTI-XA (CLEXANE OR ENOXAPARIN)
1 x Sodium Citrate To Myaree lab within 4 hours - transport in blue bag. Or single spin/separate/freeze and transport frozen. Record date and time of last dose on form. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
ANTI-XA (FRAGMIN OR DALTEPARIN)
1 x Sodium Citrate To Myaree lab within 4 hours. Transport in blue bag. Or single spin, separate and freeze and transport frozen. Record date and time of last dose on form. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
ANTI-XA (OTHER) 1 x Sodium Citrate To Myaree lab within 4 hours - transport in blue bag. Or single spin/separate/freeze and transport frozen. Record anticoagulant type and date/time of last dose on form or get patient to complete questionnaire FRM-COLL-43. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
Specimen Collection Manual Issue 19 Page 9 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
ANTI-XA (UNFRACTIONATED HEPARIN)
1 x Sodium Citrate To Myaree lab within 4hours - transport in blue bag. Or single spin/separate/freeze and transport frozen. Record Heparin dosing details on form. (Date/time started and/or ceased and drug name) See: FRM- COLL-86 - Coagulation Collection Guide
2.7mL
APCR See ACTIVATED PROTEIN C RESISTANCE entry for collection requirements. See: FRM- COLL-86 - Coagulation Collection Guide
APOLIPOPROETIN A & B 1 x SST Referred to: PathWest FSH. Naturopaths requests referred to Laverty Pathology.
2mL
APOLIPOPROTEIN E GENOTYPE Yes 1 x EDTA 6mL - Pink, dedicated tube
Referred to: Genomics Diagnostics 4mL
APRI SCORE 1 x SST and 1 x EDTA
APRI Score is a calculated index based on AST and platelet count
2mL
APTT See ACTIVATED PARTIAL THROMBOPLASTIN TIME entry for Collection requirements. See: FRM-COLL-86 - Coagulation Collection Guide
AQUAPORIN 4 ANTIBODIES 1 x SST Referred to: PathWest QEII 3mL
ARBOVIRUS SEROLOGY 1 x SST 1mL
ARRAY CGH - SEE MICROARRAY
ARSENIC BLOOD 1 x Trace Element Free - DO NOT SPIN
Referred to: Laverty Pathology Place DO NOT SPIN sticker around the lid.
4mL
ARSENIC URINE Yes 1 x Urine Container - random
Referred to: Laverty Pathology 20mL
ARTERIAL BLOOD GAS DOCTOR COLLECT Test is performed at the hospital sites and must be analysed immediately. Please refer the patient to the nearest hospital.
0.5mL
ASCA 1 x SST Referred to: QML Pathology 1ml
ASCITIC FLUID BIOCHEMISTRY DOCTOR COLLECT 2mL
ASOT 1 x SST Referred to: QML Pathology 1mL
Specimen Collection Manual Issue 19 Page 10 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
ASPERGILLUS PRECIPITINS 1 x SST Referred to: PathWest QEII 2mL
ASPERGILLUS PRECIPITINS PCR 1 x EDTA - DO NOT SPIN
Referred to: PathWest QEII Place DO NOT SPIN sticker around the lid.
2mL
AST (SGOT) 1 x SST 2mL
ATYPICAL RESPIRATORY SEROLOGY 1 x SST Duty Microbiologist to decide what tests to be done.
2mL
AUTOANTIBODIES 1 x SST 1mL
AUTOCLAVE VALIDATION DOCTOR COLLECT DO NOT REFRIGERATE
AUTOIMMUNE SCREEN 1 x SST 1mL
AVIAN PRECIPITINS SEROLOGY 1 x SST Referred to: PathWest QEII 2mL
B CELL REARRANGEMENT 1 x EDTA 6mL - Pink, dedicated tube
Referred to: PathWest QEII 4mL
B MEMORY CELLS 1 x EDTA - dedicated tube required
Referred to: PathWest QEII Sample must be at PathWest Immunology within 24 hours of collection.
3mL
B12 1 x SST 2mL
B12 + FOLATE 1 x SST 2mL
B19 1 x SST 2mL
B2 GLYCOPROTEIN 1 ANTIBODIES 1 x SST Referred to: QML 2mL
B9 1 x SST 2mL
BAL 1 x Fluoride Oxalate – DO NOT OPEN
Must be a pristine Fluoride Oxalate/SST tube – place DO NOT OPEN sticker around the lid. Alcohol swab must not be used to clean area. Police/legal purpose sample Western Diagnostic Pathology is not licensed to perform Blood Alcohol analysis for police or legal purposes. Such samples (usually in a sealed envelope) cannot be accepted. Do not accept the samples and advise the patient to contact the Forensic Science at ChemCentre on 08 9422 9800 for cost and instruction.
2mL
BARMAH FOREST SEROLOGY 1 x SST 1mL
BARTONELLA HENSELAE 1 x SST Referred to: PathWest QEII 5ml
BARTONELLA PCR 1 x EDTA - dedicated tube required
4mL
Specimen Collection Manual Issue 19 Page 11 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
BCR ABL 2 x EDTA - dedicated tube required
Referred to: PathWest FSH Only collect in Monday – Thursday. Do not collect on Public Holidays.
BEE VENOM BLOCKING ANTIBODIES
1 x SST Referred to: SA Pathology (Formally known as IMVS Pathology)
2mL
BENCE JONES PROTEIN 1 x Urine Container - random or 1 x Urine Container- 24hr plain
See Urine protein electrophoresis 7ml
BENZENE URINE 1 x Urine Container - random
Referred to: WorkCover NSW Midstream urine collection
10mL
BERYLLIUM - BLOOD Yes 1 x Trace Element Free - DO NOT SPIN
Referred to: Laverty Pathology Place DO NOT SPIN sticker around the lid.
6mL
BERYLLIUM - URINE Yes 1 x Urine Container - random
Referred to: Laverty Pathology 20mL
BETA 2 MICROGLOBULIN SERUM (B2M)
1 x SST NOTE: This is not URINE BETA 2 MICROGLOBULIN.
1mL
BETA 2 MICROGLOBULIN URINE Yes 1 x Urine Container - random
Referred to: Royal Brisbane Hospital Keep refrigerated. Please send on Ice Brick.
5mL
BETA HYDROXYBUTYRATE 1 x Lithium Heparin spin, separate & freeze ASAP
Referred to: PathWest QEII 1mL
BETA TRACE PROTEIN 1 x Urine Container
Referred to: PathWest QEII Sample is usually liquid leaking from Ear/Eyes/Nose.
1mL
BETHESDA ASSAY See INHIBITOR SCREEN entry for Collection requirements. See: FRM- COLL-86 - Coagulation Collection Guide
BHCG (QUALITATIVE) 1 x SST 2mL
BHCG (QUANTITATIVE) (URGENT – Result within 4hours)
1 x SST Do not mark urgent unless: 1. Marked urgent by the doctor 2. Clinical notes indicate query
ectopic 3. Clinical notes indicate query
miscarriage
2mL
BHCG (TUMOUR MARKER) 1 x SST 2mL
BICARBONATE 1 x SST 2mL
Specimen Collection Manual Issue 19 Page 12 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
BILE ACID/ SALTS 1 x SST Referred to: PathWest QEII Fasting unless otherwise indicated by the doctor.
2mL
BILE SALTS (FASTING & POST PRANDIAL)
2 x SST Referred to: PathWest QEII 1. Patient must be fasting. 2. Collect 1x SST labelling time of
collection and 'fasting' on tube. 3. Patient must return two hours
after having a meal. 4. Collect the second SST with time
of collection and ' post ' written on tube.
2mL
BILIRUBIN 1 x SST 2mL
BILIRUBIN (CONJUAGED NEONATAL)
1 x Plain Tube (Red Top)
1mL
BILIRUBIN (CONJUGATED) 1 x SST 2mL
BILIRUBIN (FLUID) DOCTOR COLLECT 2mL
BILIRUBIN (NEONATAL) 1 x Plain Tube (Red Top)
The preferred sample type for measurement of bilirubin in neonates and infants up to 1 month of age is a Plain Tube (Red Top).
1mL
BISMUTH Yes 1 x Trace Element Free Tube or 1 x EDTA
Referred to: Royal North Shore Hospital
2mL
BK VIRAL LOAD 1 x EDTA - dedicated tube required or Urine Container - random
Referred to: PathWest QEII 1mL
BK VIRUS PCR 1 x EDTA - dedicated tube required or Urine Container - random
Referred to: PathWest QEII 1mL
BLADDER WASHINGS DOCTOR COLLECT Keep refrigerated and send to Cytology Dept. as soon as possible.
BLEEDING STUDIES OR SCREEN See FACTOR STUDIES entry for collection requirements. See: FRM- COLL-86 - Coagulation Collection Guide
Specimen Collection Manual Issue 19 Page 13 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
BLEEDING TIME (URGENT – Result within 4hours)
See PLATELET FUNCTION ANALYSIS entry for collection requirements. See: FRM-COLL-86 - Coagulation Collection Guide
BLOOD CULTURE Blood Culture Bottles
DO NOT REFRIGERATE See: WI-COLL-74 - Blood Culture Collection
BLOOD GROUP 1 x EDTA 6mL - Pink, dedicated tube
1. Full Name, DOB or URN, DOC & TOC must be on EDTA tube and request form. All information must match between form and sample.
2. Collector must sign the tube and certifier on form - these signatures must match.
Contact Transfusion Medicine Dept. on 08 9321 3300 for any enquiries.
6mL
BLOOD GROUP & ANTIBODY SCREEN
1 x EDTA 6mL - Pink, dedicated tube
1. Full Name, DOB or URN, DOC & TOC must be on EDTA tube and request form. All information must match between form and sample.
2. Collector must sign the tube and certifier on form - these signatures must match.
Contact Transfusion Medicine Dept. on 08 9321 3300 for any enquiries.
6mL
BLOOD PRESSURE MONITOR/ 24 HR ABP MONITOR
Yes See WI-COLL-55 Provide FRM-COLL-99 to patient Done at specific sites only by phone appointment. See FRM-COLL-102 - Specialised Test Locations
BNP Yes 1 x EDTA - spin, separate & freeze
Spin, separate & freeze within 24hrs Testing performed at Joondalup Laboratory
0.5mL
BONE MARROW CYTOGENETICS DOCTOR COLLECT Referred to: PathWest FSH Bone Marrow in VTM
BONE MARROW TREPHINE DOCTOR COLLECT Done by Pathologist. Keep all samples collected by the doctor together until processed by SRA, who will then distributed to the separate departments.
BONE PROFILE 1 x SST and 1 x PPT spin within 30 minutes of collection
See: WI-COLL-113 Vitamin D Bulk Billing Criteria
2mL
Specimen Collection Manual Issue 19 Page 14 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
BONE SPECIFIC ALP 1 x SST 1mLBORDETELLA PERTUSSIS PCR
1 x Dry Swab - Orange/White
See WI-COLL-115 and FRM-COLL-81 (Swab Collection guide)
BORDETELLA PERTUSSIS SEROLOGY
1 x SST 4mL
BOWEL CANCER MARKER 1 x SST 2mL
BRAOVO Yes 2 x EDTA - dedicated tube required
Collect 2x EDTA 10 minutes apart and ensure patient identity checked each time.
Refer patient to contact Genomic Diagnostics on 1800 822 999 for any pricing queries.
4mL each
BRCA 1 & 2 Yes 2 x EDTA - dedicated tube required
Collect 2x EDTA 10 minutes apart and ensure patient identity checked each time.
Refer patient to contact Genomic Diagnostics on 1800 822 999 for any pricing queries.
4mL each
BREAST TUMOUR MARKER 1 x SST 2mL
BREATH ALCOHOL TEST Yes See: WI-COLL-53 - Drugs of Abuse Collection Manual
N/A
BROMIDE Yes 1 x Trace Element Free Tube - DO NOT SPIN
Referred to: Royal North Shore Hospital Place DO NOT SPIN sticker around the lid.
2mL
BRONCHIAL WASHINGS/BRUSHINGS
DOCTOR COLLECT Keep refrigerated and send to Cytology Dept. as soon as possible.
BRONCHOALVEOLAR LAVAGE DOCTOR COLLECT Keep refrigerated and send to Cytology Dept. as soon as possible.
BRUCELLA SEROLOGY 1 x SST 2mL
BSL (ACC) 1 x SST Serum must be spun within 4 hours of collection. No exception. If unable to spin on site, collect a FLOX.
2mL
BSL (DOCTOR/NURSING HOME/MOBILE)
1 x Fluoride Oxalate
2mL
BUN (BLOOD UREA NITROGEN) 1 x SST 2mL
C1 ESTERASE INHIBITOR LEVEL 1 x SST - Spin and freeze
Referred to: QML Pathology. Must be frozen within 1 hour of collection into 2 separate 1mL aliquots.
2mL
Specimen Collection Manual Issue 19 Page 15 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
C2H50H - ALCOHOL 1 x Fluoride Oxalate - DO NOT OPEN
Must be a pristine Fluoride Oxalate/SST tube – place DO NOT OPEN sticker around the lid. Alcohol swab must not be used to clean area. Police/legal purpose sample Western Diagnostic Pathology is not licensed to perform Blood Alcohol analysis for police or legal purposes. Such samples (usually in a sealed envelope) cannot be accepted. Do not accept the samples and advise the patient to contact the Forensic Science at ChemCentre on 08 9422 9800 for cost and instruction.
2mL
C3 1 x SST 2mL
C3 NEPHRITIC FACTOR 1 x SST spin separate and freeze and 1 x Chilled EDTA - Spin, separate and freeze
Referred to: Immunology Department Flinders Medical Centre SA Pathology Freeze serum & EDTA plasma within 2hrs of collection.
0.5mL each
C4 1 x SST 2mL
CA 27.29 1 x SST 2mL
CA 72.4 / 74 Yes 1 x SST - Spin and freeze
Referred to: Royal Prince Alfred Hospital
2mL
CA125 1 x SST 2mL
CA153 1 x SST 2mL
CA199 1 x SST 2mL
CACNA1S 1 x EDTA - dedicated tube required
Referred to: PathWest FSH Stability: 1 Week
2mL
CADASIL 1 x EDTA - dedicated tube required
Referred to: PathWest QEII Stability: 1 week
2mL
CADMIUM BLOOD 1 x Trace Element Free - DO NOT SPIN
Referred to: Laverty Pathology Place DO NOT SPIN sticker around the lid.
2mL
CADMIUM URINE 1 x Urine Container - random
Referred to: Laverty Pathology To avoid contamination - advise patients that dust from clothing does not come into contact with the urine during collection.
20mL
Specimen Collection Manual Issue 19 Page 16 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
CAERULOPLASMIN 1 x SST Referred to: QML Pathology 0.3mL
CAH SCREENCALCITONIN 1 x Lithium Heparin spin, separate & freeze ASAP
Referred to: PathWest FSH 1mL
CALCIUM 1 x SST Do not use torniquet for calcium collection.
2mL
CALCIUM (24 HOUR URINE) 1 x Urine Container- 24hr acid
Must be acidified. 2mL
CALCIUM (FLUID) DOCTOR COLLECT 2mL
CALCIUM (SPOT URINE) 1 x Urine Container - random
Does not need to be acidified 2mL
CALCIUM/CREATININE RATIO (SPOT URINE)
1 x Urine Container - random
Transfer into Vacutainer (no additive) urine tube. Urine tubes must not be filled beyond half way to prevent spillage.
2mL
CALIBRATED AUTOMATED THROMBOGRAM (CAT)
2 x Sodium Citrate To Myaree lab within 4hrs - transport in blue bag. Or DOUBLE spin/separate/freeze and transport frozen. Record anticoagulant name, date and time of last dose or patient to complete questionnaire: FRM- COLL-43 See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
CALRETICULIN 2 x EDTA 6mL - Pink, dedicated tube
Referred to: Genomics Diagnostics When co-requested with JAK 2 Mutation and/or MPL Mutation only 2 EDTA 6ml Pink tubes required altogether.
4mL
CANCER ASSOCIATED SERUM ANTIGEN
CANDIDA SEROLOGY 1 x SST Referred to: Laverty Pathology 5mL
CAPILLARY BLOOD GAS DOCTOR COLLECT Test is performed at the hospital sites and must be analysed immediately. Please refer the patient to the nearest hospital.
0.5mL
Specimen Collection Manual Issue 19 Page 17 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
CARBAMAZEPINE 1 x SST Record date and time of last dose of medication on the form.
2mL
CARBAPENEM-RESISTANT ENTEROBACTERIACEAE (CRE SCREEN)
Yes PATIENT COLLECT or Faeces Container
Separate rectal swabs required for CRE screen if requested with VRE screen (i.e. 2 separate rectal swabs required.) Rectal swab will be Patient Collect - Provide patient with Blue Top Gel swab. DO NOT REFRIGERATE swabs but refrigerate faeces.
CARBOXYHAEMOGLOBIN DOCTOR COLLECT Test is performed at the hospital sites and must be analysed immediately. Please refer the patient to the nearest hospital.
0.5mL
CARCINOEMBRYONIC ANTIGEN 1 x SST 2mL
CARDIAC ENZYMES (URGENT – Result within 2hours)
See TROPONIN
CARDIOLIPIN ANTIBODIES 1 x SST For Antiphospholipid Antibodies see also Lupus Anticoagulant and B2 Glycoprotein 1 Antibodies
1mL
CARNITINE 1 x SST spin separate and freeze
Referred to: PathWest QEII 1mL
CAT See CALIBRATED AUTOMATED THROMBOGRAM entry for Collection requirements. See: FRM-COLL-86 - Coagulation Collection Guide
CAT SCRATCH PCR 1 x EDTA - dedicated tube required
Referred to: PathWest QEII 2mL
CATECHOLAMINES BLOOD 2 x Lithium Heparin spin, separate & freeze ASAP
Referred to: PathWest FSH Overnight fasting required (can have water). Patient should be supine (lying down) for 30mins prior to collection. Please record supine duration on request form. Avoid caffeine. Document drug history if possible.
1.5mL
CATECHOLAMINES URINE 1 x Urine Container- 24hr acid
Referred to: PathWest FSH Spot urine if child – SRA to add acid till it changes to pH to 3. The patient should ideally refrain from taking medication for 24 hours prior to, and during urine collection and avoid physical and physiological stress prior to testing.
4mL
Specimen Collection Manual Issue 19 Page 18 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
CB2 EPOTIDE Test Not Available
CBA See FACTOR STUDIES entry for collection requirements. See: FRM- COLL-86 - Coagulation Collection Guide
CD 34 PROGENITOR CELLS 1 x EDTA - dedicated tube required
Referred to: PathWest QEII or FSH Always urgent. Needs to be forwarded directly to bone marrow transplant lab.
2mL
CD MARKERS (EG. CD3, CD4, CD8, CD19, CD20, ETC)
1 x ACD and 1 x EDTA
Full tube of ACD (can use 1mL of EDTA ONLY if paediatric or elderly/infirm).
6mL ACD
CD3-/CD57+ Yes 1 x ACD and 1 x EDTA
Full tube of ACD (can use 1mL of EDTA ONLY if paediatric or elderly/infirm). See: WI-COLL-96 - Flow Cytometry
6mL ACD
CD4 1 x ACD and 1 x EDTA
Full tube of ACD (can use 1mL of EDTA ONLY if a very difficult collect). See: WI-COLL-96 - Flow Cytometry
6mL ACD
CDT Yes 1 x SST spin separate and freeze
Referred to: QML pathology 1mL
CEA 1 x SST 2mL
CEPHALASPORIN RAST 1 x SST Referred to: QML Pathology 2mL
CH 50 1 x SST - Spin, Separate into 2 aliquots & Freeze
Referred to: PathWest QEII Must be frozen within 1 hour of collection into 2 separate 1mL aliquots.
2mL
CHAGAS 1 x SST Referred to: ICPMR Westmead Hospital (NSW)
2mL
CHANCROID PCR 1 x Dry Swab - Orange/White
Referred to: PathWest QEII Only from an ulcer and must note that it is from ulcer on the request form
CHANNEL WATER TESTING Urine Container DO NOT REFRIGERATE
CHARCOT MARIE TOOTH DISEASE 1 x EDTA - dedicated tube required
Referred to: PathWest FSH 2mL
Specimen Collection Manual Issue 19 Page 19 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
CHIKUNGUNYA 1 x SST Referred to: PathWest QEII 2mL
CHIKUNGUNYA PCR 1 x SST This test although run via PathWest is not validated. The PCR lab will check with the doctor before performing the test.
2mL
CHLAMYDIA RESPIRATORY SEROLOGY
1 x SST Referred to: QML Pathology 2mL
CHLAMYDIA/GONORRHOEA SWABS & URINE FOR PCR/NAAT
1 x Swab - Cobas or 1 x Urine - Cobas tube
See WI-COLL-115 and FRM-COLL-81 URINE: First 20-30mL of urine in sterile yellow top container. Urine COBAS collection tube required. Please note swabs collected from genital sites are to be collected by referring doctor.
CHLAMYDOPHILA PNEUMONIA PCR
1 x Dry Swab - Orange/White
See WI-COLL-115 and FRM-COLL-81 (Swab Collection guide)
CHLORIDE 1 x SST 2mL
CHLORIDE (FLUID) DOCTOR COLLECT 2mL
CHLORPROMAZINE 1 x Plain Tube (Red Top)
Referred to: Royal Brisbane Hospital 3mL
CHOLESTEROL AND CORONORY RISK RATIO
1 x SST See: WI-COLL-61 - Lipid Testing 2mL
CHOLESTEROL AND CRR 1 x SST See: WI-COLL-61 - Lipid Testing 2mL
CHOLESTEROL AND FRAMINGHAM RATIO
1 x SST See: WI-COLL-61 - Lipid Testing 2mL
CHOLESTEROL AND HDL 1 x SST See: WI-COLL-61 - Lipid Testing 2mL
CHOLESTEROL AND LDL 1 x SST See: WI-COLL-61 - Lipid Testing 2mL
CHOLESTEROL AND LIPID SCREEN 1 x SST See: WI-COLL-61 - Lipid Testing 2mL
CHOLESTEROL AND LIPOPROTEINS 1 x SST See: WI-COLL-61 - Lipid Testing 2mL
CHOLESTEROL AND TRIGLYCERIDES
1 x SST See: WI-COLL-61 - Lipid Testing 2mL
CHOLINESTERASE RED CELL 1 x EDTA - dedicated tube required
Referred to: QML Pathology 4mL
CHOLINESTERASE SERUM 1 x SST - dedicated tube required
Referred to: QML Pathology
CHROMATIN ANTIBODIES 1 x SST Referred to: Laverty Pathology Only if ANA is positive.
1mL
Specimen Collection Manual Issue 19 Page 20 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
CHROMIUM BLOOD 1 x Trace Element Free - spin & separate
Referred to: Laverty Pathology
6mL
CHROMIUM URINE 1 x Urine Container - random
Referred to: Laverty Pathology To avoid contamination- advise patients that dust from clothing does not come into contact with the urine during collection.
20mL
CHROMOGRANIN A Yes 1 x SST spin separate and freeze
Referred to: QML Pathology 5mL
CHROMOSOMES - AMNIOTIC FLUID (AF)
DOCTOR COLLECT
Referred to: QML Pathology Contact Genetic Dept. 08 9317 0922 for any queries
CHROMOSOMES - BLOOD 1 x Lithium Heparin - DO NOT SPIN
Referred to: QML Pathology Place DO NOT SPIN sticker around the lid.
6mL
CHROMOSOMES - CHORIONIC VILLI (CVS)
DOCTOR COLLECT
Referred to: QML Pathology Contact Genetic Dept. 08 9317 0922 for any queries
CHROMOSOMES – LEUKAEMIA (CML, CLL)
1 x Lithium Heparin
1mL
CHROMOSOMES - PRODUCT OF CONCEPTION (POC)
DOCTOR COLLECT
Referred to: QML Pathology No Formalin. Specialised container: 70mL red top sterile jar containing PBS and heparin. Processed at QML.
CHRONIC FATIGUE SYNDROME Refer to AMMINO ACID SCREEN
CITRATE URINE 1 x Urine Container- 24hr acid
Referred to: PathWest FSH Patient must collect over 24-hour period.
CK 1 x SST 2mL
CKMB 1 x SST Referred to: QML Pathology 2mL
CLN 2 GENETIC TEST 1 x EDTA - dedicated tube required
Referred to: Adelaide Women's and Children's Hospital Collect sample Monday - Wednesday only. Must be at Adelaide Women's and Children's Hospital within 48hrs.
Specimen Collection Manual Issue 19 Page 21 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
CLOBAZAM 1 x Plain Tube (Red Top)
Referred to: QML Pathology Collect just prior to the next dose or at least 6 hours after the last dose. Provide clinical and medication detail, including time and date of last dose.
2mL
CLOMIPRAMINE 1 x Plain Tube (Red Top)
Referred to: QML Pathology Collect just prior to the next dose or at least 6 hours after the last dose. Provide clinical and medication detail, including time and date of last dose.
2mL
CLONAZEPAM 1 x Plain Tube (Red Top)
Referred to: QML Pathology Collect just prior to the next dose or at least 6 hours after the last dose. Provide clinical and medication detail, including time and dose of last dose.
2mL
CLOSTRIDIUM DIFFICILE PCR 1 x Faeces Container
Provide patient with FRM-COLL-13
CLOZAPINE 1 x Plain Tube (Red Top)
Referred to: QML Pathology Collect just prior to the next dose or at least 8 hours after the last dose. Provide clinical and medication detail, including time and dose of last dose.
2mL
CMP (CALCIUM, MAGNESIUM, PHOSPHATE)
1 x SST Do not use torniquet for calcium collection.
2mL
CMV 1 x SST 2mL
CMV PCR BLOOD 1 x EDTA - dedicated tube required
Referred to: PathWest QEII 2mL
CMV PCR URINE 1 x Urine Container - dedicated jar required. Freeze ASAP
Referred to: Royal North Shore Hospital (NSW)
10mL
CMV VIRAL LOAD 1 x EDTA - dedicated tube required
Referred to: PathWest FSH Spot urine sample may be collected for 3 years and under.
COAGULATION PROFILE 2 x Sodium Citrate To lab within 10 hours - transport in a blue bag. Or single spin/separate/freeze and transport frozen. Patient to complete Questionnaire: FRM-COLL-43. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
COBALT BLOOD 1 x Trace Element Free - spin & separate
Referred to: Laverty pathology 6mL
Specimen Collection Manual Issue 19 Page 22 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
COBALT URINE 1 x Urine Container - random
Referred to: Laverty Pathology. To avoid contamination - advise patients that dust from clothing does not come into contact with the urine during collection.
20mL
CODEINE Yes 1 x Plain Tube (Red Top)
Referred to: QML Pathology 2mL
COELIAC DISEASE GENE TESTING Yes
1 x EDTA 6mL - Pink, dedicated tube
Referred to: Genomic Diagnostics Most patients bulk billed. Advise patient to contact Genomic Diagnostics on 1800 822 999 for pricing queries.
6mL
COELIAC SEROLOGY 1 x SST 1mL
CO-ENZYME Q10 1 x Lithium Heparin - Spin, Separate, Foil & Freeze ASAP
Referred to: Royal Brisbane Hospital 2mL
COLD AGGLUTININS
1 x EDTA and 1 x Plain Tube (Red Top)
1. Cold agglutinins samples can ONLY be collected in centres with a water bath and a centrifuge
2. Warm the tubes in water bath (@37°C) for 10-20 mins prior to collecting samples
3. Collect blood into pre-warmed tubes
4. Once collected leave EDTA whole – DO NOT SPIN
5. Place plain tube only into water bath for 30 mins or until clotted
6. Spin the plain tube only and immediately separate the serum from the plain tube into tip off tube and label serum sample
7. Mark on the form that the sample was collected and maintained at 37°C until separated
8. Send all tubes and tip off tube with forms to Myaree Laboratory
For any queries please contact Transfusion Medicine Dept. on 08 9321 3300
6mL
Specimen Collection Manual Issue 19 Page 23 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
COLLAGEN BINDING ASSAY See FACTOR STUDIES entry for collection requirements. See: FRM- COLL-86 - Coagulation Collection Guide
COM 1 x SST 2mL
COMPLEMENT LEVELS 1 x SST 2mL
COMPLEMENTS C1Q - C2 1 x SST - Spin, Separate into 3 aliquots & Freeze
Referred to: SEALS Pathology (NSW) Must be frozen within 1 hour of collection into 3 separate aliquots.
2mL
CONFIRMATION TEST (CALL BEFORE COLLECTION)
Yes Call Toxicology Dept. on 08 9317 0849 before collection. Full Chain of Custody Collect This maybe requested to be confirmed off a previous episode and the donor may be just here to pay for the extra testing. Please contact toxicology once payment has been made. See: WI-COLL-53 - Drugs of Abuse Collection Manual
5mL each
CORD AND MATERNAL BLOOD GROUP
1 x EDTA 6mL - Pink, dedicated tube and
1x Cord Blood
1. Full Name, DOB or URN, DOC & TOC must be on EDTA tube and request form. All information must match between form and sample.
2. Collector must sign the EDTA tube and certifier on form - these signatures must match.
Send baby’s cord blood and mother’s EDTA together – DO NOT SEPRATE the samples.
CORD BLOOD GROUP 1 x EDTA - dedicated tube required
DOCTOR COLLECT Full Name, DOB or URN, DOC & TOC must be on EDTA tube. Collector must sign EDTA tube and certifier on the request form. This tube is collected at the birth of the baby by medical or nursing staff. Tube must be labelled with B/O and mothers details including date & time of collect.
2mL
CORONAVIRUS 1 x Dry Swab - Orange/White
Referred to: PathWest QEII
Specimen Collection Manual Issue 19 Page 24 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
CORTISOL 1 x SST AM sample: to be collected between 0800 - 0900 am. PM sample: to be collected between 1600 - 1700 pm. Random sample: anytime and note the time of collection on the form.
2mL
CORTISOL (SALIVA) Yes Special Collection Kit
Provide patients with FRM- COLL-16 - Late Night Salivary Cortisol and the special collection tube (Salivette Tube). Collect between 11pm - midnight. Note date and time of collection on salivette tube. Salivette tube available via Manual Chemistry Dept. on 08 9317 0838
1mL
CORTISOL URINE (24 HOUR URINE)
1 x Urine Container- 24hr plain
Non-acidified urine. If a dexamethasone suppression test is requested at the same time, collect this test first and schedule the dexamethasone suppression test for when the urine is returned.
1mL
COTININE BLOOD Yes 1 x Plain Tube (Red Top)
Referred to: Laverty Pathology 1mL
COTININE URINE Yes 1 x Urine Container - random
Referred to: Laverty Pathology. 20mL
COUNSYL Yes 1 x EDTA - dedicated tube required
Referred to: Genomic Diagnostics Dedicated request and consent form required (cannot use standard request form). Pre-payment is preferred via phone 1800 822 999 (9am-5pm AEST)
3mL
COXSACKIE PCR 1 x Dry Swab - Orange/White or 1 x Faeces Container
Referred to: PathWest QEII Coxsackie serology is not available. Throat swab or rectal swab site.
C-PEPTIDE 1 x SST - Spin and freeze ASAP
Fasting specimen preferred. 1mL
CPK 1 x SST 2mL
CREATININE 1 x SST 2mL
CREATININE (24 HOUR URINE) 1 x Urine Container- 24hr plain
2mL
Specimen Collection Manual Issue 19 Page 25 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
CREATININE (FLUID) DOCTOR COLLECT 2mL
CREATININE (SPOT URINE) 1 x Urine Container - random
Transfer into Vacutainer (no additive) urine tube. Urine tubes must not be filled beyond half way to prevent spillage.
2mL
CREATININE CLEARANCE 1 x SST and 1 x Urine Container- 24hr plain
Urine and SST must be collected within 24 hours of each other.
2mL
CROHN'S DISEASE (ASCA & FAECAL CALPROTECTIN)
Yes 1 x SST and 1 x Faeces Container
ASCA referred to QML Pathology ANCA and FAECAL CALPROTECTIN performed in-house. Min volume: 1mL blood and 10g faeces
CROSSMATCH (URGENT)
1 x EDTA 6mL - Pink, dedicated tube
1. Ask the patient whether they have been transfused or pregnant in the last 3 months – note on request form.
2. Ask date of operation and which hospital it is to be performed at – note on request form.
3. Full Name, DOB or URN, DOC & TOC must be on EDTA tube and request form. All information must match between form and sample.
4. Collector must sign the tube and certifier on form - these signatures must match.
If unsure what to collect ring the Transfusion Medicine Dept. on 08 9321 3300
6mL
CRP 1 x SST 2mL
CRR 1 x SST See: WI-COLL-61 - Lipid Testing 2mL
Specimen Collection Manual Issue 19 Page 26 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
CRYOGLOBULINS/ CRYOFIBRINOGEN
1 x EDTA and 1 x Plain Tube (Red Top) - Spin & Separate
Cryoglobulins and cold agglutinins are two separate tests. Samples are to be if the cold agglutination and cryoglobulins are co-requested. 1. Warm both EDTA and plain tube to
37 oC prior to collection. 2. Collect the blood into the warmed
tubes. 3. Label both tubes with the patient
details. 4. Place the samples into the 37 oC
water bath for 30 minutes. 5. Centrifuge the samples in a non-
refrigerated centrifuge for 10 minutes.
6. Immediately separate the plasma from the EDTA and the serum from the plain tube into take-off tubes.
7. Carefully label the take-off tubes with the patient name, whether serum or plasma and “cryoglobulins".
8. Mark on the form that the samples were collected at 37 oC and maintained at that temperature until separated.
4mL each
CRYPTOCOCCAL PCR Test Not Available
CRYPTOCOCCAL SEROLOGY 1 x SST Referred to: PathWest QEII 2mL
CSF CYTOLOGY (URGENT)
DR COLLECT Keep refrigerated and send to Cytology Dept. IMMEDIATELY for processing. CSF for Cytology are always treated as URGENT. Keep specimen refrigerated. On call Histopathologist should be contacted if specimen is received out of hours.
CSF MC&S (URGENT)
3 x CSF tube (sterile)
DR COLLECT DO NOT REFRIGERATE
CSF OLIGOCLONAL BANDS 1 x SST and 1 x CSF tube (sterile)
Referred to: PathWest QEII Both a CSF and a serum specimen are required. The procedure cannot be done without a serum specimen. The CSF and serum specimen can be taken up to 1 week apart.
1mL
CTX 1 x PPT - spin and freeze ASAP
Prefer fasted and morning sample 4mL
Specimen Collection Manual Issue 19 Page 27 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
CYANIDE 1 x Lithium Heparin
Referred to: Royal Prince Alfred Hospital
5mL
CYANIDE - URINE 1 x Urine Container
Referred to: WorkCover NSW (TestSafe) Collection is to be first void urine at end of shift.
CYCLIC CITRULLINATED PEPTIDE ANTIBODY (CCP)
1 x SST 2mL
CYCLOSPORIN 1 x EDTA Referred to: PathWest QEII Therapeutic drug monitoring. Cyclosporin is an immunosuppressant drug. It is treated as an urgent sample for patients immediately post- transplant.
CYP21 MUTATION GENOTYPING 1 x EDTA - dedicated tube required
Referred to: PathWest QEII
CYSTIC FIBROSIS GENE TESTING Yes 1 x EDTA - dedicated tube required
Referred to: Genomic Diagnostics 4mL
CYSTICERCOSIS SST 0.5mL
CYSTINE (24 HOUR URINE) 1 x Urine Container- 24hr plain
Referred to: PathWest QEII
CYSTINE SCREEN (URINE) 1x Urine container - dedicated jar required. Freeze ASAP
Referred to: PathWest QEII 5mL
CYTOGENETICS See CHROMOSOMES
CYTOMEGALOVIRUS (CMV) SEROLOGY
1 x SST 2mL
D-DIMER (URGENT – Result within 4hours)
1 x Sodium Citrate See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
DENGUE PCR 1 x EDTA Referred to: PathWest QEII 3mL
DENGUE SEROLOGY 1 x SST 2mL
DEOXYPYRIDINOLINE, URINE 1x Urine container - dedicated jar required. Freeze ASAP Or 1x Urine Container - 24h plain
Referred to: Royal Prince Alfred Hospital A random early morning urine specimen is preferred. A 24-hour urine may be collected if specifically requested.
5mL
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Vol
DESIPRAMINE 1 x Plain Tube (Red Top)
Referred to: QML Pathology Collect just prior to the next dose or at least 6 hours after the last dose. Provide clinical and medication detail, including time and date of last dose.
2 mL
DESVENLAFAXINE 1 x Plain Tube (Red Top)
Referred to: PathWest QEII Take sample prior to dose This test is referred to and performed by
2 mL
DEXAMETHASONE SUPPRESSION 1 x SST 1. Confirm that patient has not taken the dexamethasone tablets yet
a. If the patient has already taken dexamethasone tablets the previous evening
b. Collect SST for dexamethasone suppression test
c. Defer all other requested tests for at least 2 days later
2. If there are other requested tests in addition to the dexamethasone suppression test.
a. Collect other tests first b. Defer the dexamethasone
suppression test for a subsequent day
3. Contact Myaree Patient Reception on 08 9317 0710 for forwarding of appropriate tablets
4. Provide patient with a. FRM-COLL-5 b. 2 x 0.5mg tablets
5. Advise patient to take the tablets between 22:00-23:00pm as per FRM-COLL-5 and return to the collection centre the next day between 08:00-9:00am
6. SST is collected the next day between 08:00-09:00 am.
2mL
DGP IGG 1 x SST 1ml
DHEA 1 x SST 1mL
DIAZEPAM 1 x Plain Tube (Red Top)
Referred to: Royal Prince Alfred Hospital
5mL
DIF DOCTOR COLLECT Deliver to Histopathology Dept. ASAP
Specimen Collection Manual Issue 19 Page 29 of 95
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Vol
DIGEORGE SYNDROME 1 x EDTA - dedicated tube required
3mL
DIGITALIS 1 x SST Record date and time of last dose of medication on the form. Bloods should be taken 6 hours post dose. Do not turn patients away if not 6 hours post dose.
2mL
DIGOXIN 1 x SST Record date and time of last dose of medication on the form. Bloods should be taken 6 hours post dose. Do not turn patients away if not 6 hours post dose.
2mL
DIHYDROTESTOSTERONE 1 x SST - Spin and freeze
Referred to: Dorevitch Pathology 2mL
DILANTIN 1 x SST Record date and time of last dose of medication on the form.
2mL
DIPHTHERIA SEROLOGY Yes 1 x SST Referred to: The Children's Hospital at Westmead
5mL
DIRECT COOMBS TEST 1 x EDTA 6mL - Pink, dedicated tube
1. Full Name, DOB or URN, DOC & TOC must be on EDTA tube and request form. All information must match between form and sample.
2. Collector must sign the tube and certifier on form - these signatures must match.
Contact Transfusion Medicine Dept. on 08 9321 3300 for any enquiries.
1mL
DNA ABS 1 x SST 1mL
DONOVANOSIS 1 x Dry Swab - Orange/White
Referred to: PathWest QEII
DOTHIEPIN (TRICYCLIC) 1 x Plain Tube (Red Top)
Referred to : QML Pathology Collect just prior to the next dose or at least 6 hours after the last dose. Provide clinical and medication detail, including time and date of last dose.
2mL
DOUBLE NEGATIVE T CELLS 1 x EDTA - dedicated tube required
Referred to : PathWest QEII Sample to be PathWest Immunology Dept. within 24hrs of collection.
4mL
DOXEPIN 1 x 1 x Plain Tube (Red Top)
Referred to: QML Pathology Collect just prior to the next dose or at least 6 hours after the last dose. Provide clinical and medication detail, including time and date of last dose.
2mL
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Vol
DRUG SCREEN (LEGAL/EMPLOYMENT/PERSONAL)
Yes Full Chain of Custody Collect Chain of Custody form MUST be completed. Please ensure you have completed all relevant fields before the donor leaves. Use company/account code or IMPA. Please attach any extra paperwork the donor comes in with to the chain of custody form. See: WI- COLL-53 - Drugs of Abuse Collection Manual
2x 5mL each
DRUG SCREEN (MEDICARE) Yes Please carefully read WI-COLL-53 to determine if the collection is actually acceptable to be covered by Medicare. If in doubt, please call Toxicology Dept. on 08 9317 0849. DAN is for rehabilitation patients only. DAM is for all other Medicare screens See: WI-COLL-53 - Drugs of Abuse Collection Manual
10mL
DSDNA ANTIBODIES 1 x SST Do not confuse with "Anti-DNAseB Antibodies" which is a referred test (see Streptococcal Serology, QSS). If unsure, please phone Immunology Dept. on 08 9317 0920
1ml
DUCT ANTIBODIES 1 x SST This test is referred to and performed by PathWest QEII
2 mL
DYSTROPHIN GENETICS TESTING 2 x EDTA - dedicated tube required
This test is referred to and performed by Fiona Stanley Hospital. MLPA analysis only. Molecular genetics reports are issued to the referring doctor only in hardcopy only utilising an independent database.
2mL
E/LFT 1 x SST 2mL
E2 1 x SST 2mL
EBV 1 x SST 2 mL
EBV PCR 1 x EDTA - dedicated tube required
This test is referred to and performed by PathWest QEII
3 mL
EBV VIRAL LOAD 1 x EDTA - dedicated tube required
Test is sent to Pathwest QEII. 2mL
Specimen Collection Manual Issue 19 Page 31 of 95
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Vol
ECG Yes Only done at Duncraig & Joondalup Hospital collection centres.
ECHINOCOCCUS SEROLOGY 1 x SST Referred to: PathWest QEII 2mL
ECP ASSAY Yes 1 x Plain Tube (Red Top) - Spin, Separate & Freeze
Referred to: Royal Prince Alfred Hospital Allow sample to clot for 2 hours before spinning.
2mL
EGFR 1 x SST 2mL
EHLERS DANLOS SYNDROME Yes 2 x EDTA 6mL - Pink, dedicated tube
Referred to: The Children's Hospital at Westmead
6mL
ELASTASE (FAECAL) Yes 1 x Faeces Container
Referred to: PathWest FSH 10g
ELECTROLYTES 1 x SST 2mL
ELECTROLYTES (URINE) 1 x Urine Container - random
2mL
ELECTROPHORESIS 1 x SST If the serum or urine is not specified next to electrophoresis then, collect both samples: 1 x SST and 1 x spot Urine.
2mL
ELEPHANTIASIS SEROLOGY 1 x SST Referred to: PathWest QEII 2mL
ENA 1 x SST SM - check as it could also be Anti Smooth Muscle - see ASM SCLERODERMA = ANF + XNA + REQ, SJOGRENS ABS = ANF + XNA + REQ
1mL
ENDOMYSIAL ANTIBODY 1 x SST 1mL
ENDOSCOPE CULTURE Urine Container DO NOT REFRIGERATE
ENTAMOEBA HISTOLYTICA SEROLOGY
1 x SST Referred to: PathWest QEII 2 mL
ENTEROVIRUS PCR 1 x Faeces Container or Dry Swab - Orange/White
Referred to: PathWest QEII This test is used in place of virus culture on faeces, throat swabs or CSF. Swabs collected in gel are not suitable for PCR testing.
ENTEROVIRUS SEROLOGY 1 x SST Referred to: QML Pathology 2 mL
EPILIM 1 x SST Record date and time of last dose of medication on the form.
2mL
EPSTEIN BARR VIRUS SEROLOGY 1 x SST 2 mL
ERYTHROPOEITIN 1 x SST spin separate and freeze ASAP
Referred to: PathWest FSH
1mL
Specimen Collection Manual Issue 19 Page 32 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
ESR/ ERYTHROCYTE SEDIMENTATION RATE (URGENT – Result within 4hours if clinically indicated)
1 x EDTA Clinical notes with Temporal arteritis treat as URGENT. Full Name, DOB or URN, DOC & TOC must be on EDTA tube. Collector must sign EDTA tube and request form. NOTE: Only 1 EDTA required for any combination of FBC/Indices, ESR, Malaria, RET, HbA1C, HE.
4ml
ESTRADIOL 1 x SST 2mL
ETHANOL 1 x Fluoride Oxalate - DO NOT OPEN
Must be a pristine Fluoride Oxalate/SST tube – place DO NOT OPEN sticker around the lid. Alcohol swab must not be used to clean area. Police/legal purpose sample Western Diagnostic Pathology is not licensed to perform Blood Alcohol analysis for police or legal purposes. Such samples (usually in a sealed envelope) cannot be accepted. Do not accept the samples and advise the patient to contact the Forensic Science at ChemCentre on 08 9422 9800 for cost and instruction.
2mL
ETHOSUXIMIDE 1 x Plain Tube (Red Top)
Referred to: Royal Brisbane Hospital Provide clinical and medication details. Keep sample cold.
2mL
ETHYLBENZENE MANDELIC ACID Yes 1 x Urine Container - random
Referred to: WorkCover NSW
20 mL
ETHYLENE GLYCOL 1 x Plain Tube (Red Top)
Referred to: Royal Prince Alfred Hospital
2mL
ETOH 1 x Fluoride Oxalate - DO NOT OPEN
Must be a pristine Fluoride Oxalate/SST tube – place DO NOT OPEN sticker around the lid. Alcohol swab must not be used to clean area. Police/legal purpose sample Western Diagnostic Pathology is not licensed to perform Blood Alcohol analysis for police or legal purposes. Such samples (usually in a sealed envelope) cannot be accepted. Do not accept the samples and advise the patient to contact the Forensic Science at ChemCentre on 08 9422 9800 for cost and instruction.
2mL
Specimen Collection Manual Issue 19 Page 33 of 95
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Vol
EUC (ELECTROLYTES, UREA AND CREATININE)
1 x SST 2mL
EVEROLIMUS 1 x EDTA - dedicated tube required
Referred to: PathWest QEII 1mL
EXON 12 MUTATION 2 x EDTA 6mL - Pink, dedicated tube
Referred to: Royal Melbourne Hospital 4mL
EYE SWAB MC&S Swab - Blue Top Gel
DO NOT REFRIGERATE
FACTOR II 2 x Sodium Citrate Referred to: PathWest FSH Must be to Myaree lab within 10hours - transport in Blue bag. Or single spin/separate/freeze and transport frozen. Patient to complete questionnaire: FRM-COLL-43. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
FACTOR INHIBITORS See INHIBITOR SCREEN entry for Collection requirements. See: FRM- COLL-86 - Coagulation Collection Guide
FACTOR IX See FACTOR STUDIES entry for collection requirements. See: FRM- COLL-86 - Coagulation Collection Guide
FACTOR STUDIES 3 x Sodium Citrate To Myaree within 10hours - transport in a blue bag. Or single spin/separate/freeze and transport frozen. Patient to complete Questionnaire: FRM-COLL-43. NOTE: This panel incorporates any of the following tests - VWF antigen, Risocetin CoFactor, Collagen binding assay (CBA), Factors VIII, IX, XI and/or XII. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
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Vol
FACTOR V 2 x Sodium Citrate Referred to: PathWest FSH Must be to Myaree within 10hrs - transport in blue bag. Or single spin/separate/freeze and transport frozen. Patient to complete questionnaire: FRM-COAG-43. NOTE: This is a different test to Factor V Leiden. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
FACTOR V LEIDEN GENE MUTATION
Yes 1 x EDTA 6mL - Pink, dedicated tube
Referred to: Genomic Diagnostics This test is performed in same assay with Factor II/Prothrombin gene mutation. The same charge applies whether testing for just Factor V Leiden or together with Factor II/Prothrombin gene. Eligible for Medicare rebate if patient has had a proven venous thrombosis or pulmonary embolism or has a first degree relative with the mutation.
1mL
FACTOR VII 2 x Sodium Citrate Referred to: PathWest FSH Must be to Myaree within 10hours - transport in blue bag. Or single spin/separate/freeze and transport frozen. Patient to complete questionnaire: FRM-COAG-43. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
FACTOR VIII See FACTOR STUDIES entry for collection requirements. See: FRM- COLL-86 - Coagulation Collection Guide
FACTOR X 2 x Sodium Citrate Referred to: PathWest FSH Must be to Myaree within 10hours - transport in blue bag. Or single spin/separate/freeze and transport frozen. Patient to complete questionnaire: FRM-COAG-43. NOTE: This is a different test to Anti-Xa. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
FACTOR X-ACTIVATED CLOTTING TIME
2 x Sodium Citrate Referred to: PathWest FSH Must be at the lab within 4hours -transport in blue bag. Or double spin/separate/freeze and transport frozen. Patient to complete questionnaire: FRM-COLL-43. Complete FRM-COLL-43 See: FRM- COLL-86 - Coagulation Collection Guide
2.7mL
Specimen Collection Manual Issue 19 Page 35 of 95
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Vol
FACTOR XI See FACTOR STUDIES entry for collection requirements. See: FRM- COLL-86 - Coagulation Collection Guide
FACTOR XII See FACTOR STUDIES entry for collection requirements. See: FRM- COLL-86 - Coagulation Collection Guide
FACTOR XIII 2 x Sodium Citrate Referred to: PathWest FSH Must be at Myaree within 10hours - transport in blue bag. Or single spin/separate/freeze and transport frozen. Patient to complete questionnaire: FRM-COAG-43. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
FAECAL CALPROTECTIN Yes Faeces Container 10g
FAECAL HAEMOGLOBIN (HUMAN) 1 x Faeces Container or 2 x Faeces Container or 3 x Faeces Container
Faecal Occult Blood (FOB) Testing is usually performed on 3 specimens to increase the sensitivity of the testing. Prior to collection provide the patient a copy of FRM-COLL-13 (Faecal Specimen Collection Patient Information sheet) to assist with the collection procedure. (Don’t forget to give the patient enough brown topped faeces jars and specimen bags). If the request is for multiple samples, they need to be collected on separate days. The patient should be instructed to return the samples as they are collected. Alternatively, they may be kept in the fridge (2-8°C) for up to 72 hours. See: WI- COLL-80 - Faecal Occult Blood
FAECAL LACTOSE INTOLERANCE Faeces Container Referred to: QML Pathology Sample sent as REDUCING SUBSTANCES in children under 11 years old. Not available over this age
FAECAL MULITPLEX PCR 1 x Faeces Container
See WI-COLL-115 and FRM-COLL-81 (Swab Collection guide) Tests for the following pathogens by PCR: Salmonella species, Campylobacter, Shigella, Aeromonas, Yersinia enterolytica, Giardia, Crypstoporidium, Dientamoeba fragili, Blastocystis hominis & Entamoeba histolytica.
Specimen Collection Manual Issue 19 Page 36 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
FAECES - LISTERIA CULTURE 1 x Faeces Container
See: WI-COLL-78 - Faeces MC&S and Multiplex PCR
FAECES MICROSCOPY - FAT GLOBULES
1 x Faeces Container
FAECES MICROSCOPY ONLY (OCP) Faeces Container See Faeces for Parasite WI
FAECES OCP/MC&S Faeces Container See: WI-COLL-78 - Faeces MC&S and Multiplex PCR
FAMILIAL HYPERCHOLESTEROLAEMIA DNA STUDIES
The RPH Lipid Disorders Clinic runs the state FH service and can be contacted on 9224 8092, 9224 1319 (fax), or [email protected] Please instruct the patient/doctor to call the above number and DO NOT COLLECT SAMPLES.
FAMILIAL MEDITERRANEAN FEVER Yes 2 x EDTA 6mL - Pink, dedicated tube
Referred to: PathWest QEII 9 mL
FARMERS LUNG SEROLOGY 1 x SST Referred to: PathWest QEII 2mL
FASCIOLA HEPATICA SEROLOGY 1 x SST Referred to: ICPMR Westmead Hospital (NSW)
2mL
FDP See D-DIMER entry for collection requirements. See: FRM-COLL-86 - Coagulation Collection Guide
FE/ FE STUDIES 1 x SST 2mL
FEMALE SEX HORMONES 1 x SST 2mL
FERRITIN 1 x SST 2mL
FES 1 x SST 2mL
FIB4 1 x SST and 1 x Sodium Citrate
2mL
FIBRIN DEGRADATION PRODUCTS See D-DIMER entry for collection requirements. See: FRM-COLL-86 - Coagulation Collection Guide
FIBRINOGEN 2 x Sodium Citrate To lab within 10hours. Transport in a Blue bag. Otherwise, single spin, separate and freeze and transport frozen. Patient to complete questionnaire: FRM-COLL-43. See: FRM-COLL-86 - Coagulation Collection Guide
2.7 mL
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Vol
FIBROBLAST GROWTH FACTOR 23 Test Not Available
FILARIA BLOOD PARASITES (URGENT – Result within 4hours)
1 x EDTA Full Name, DOB or URN, DOC & TOC must be on EDTA tube. Collector must sign EDTA tube and request form. NOTE: Only 1 EDTA required for any combination of FBC/Indices, ESR, Malaria, RET, HbA1C, HE, FLM.
1ml
FILARIASIS SEROLOGY 1 x SST Referred to: ICPMR Westmead Hospital (NSW)
2 mL
FIP1L1-PDGFRA 1 x EDTA 6mL - Pink, dedicated tube or Bone Marrow in EDTA
Referred to: PathWest QEII Collect on Monday to Thursday. Do not collect on Public Holidays.
FIRST TRIMESTER SCREEN (FTS) 1 x SST - Spin and freeze ASAP
Acceptable gestation age: 8 weeks 0 days to 13 weeks 6 days. Fill out Antenatal Patient Questionnaire (FRM-COLL-6). Please note- out of pocket fee of $30 no longer applicable. Bulk billed.
4 mL
FIRST TRIMESTER SCREEN (SAMSAS)
1 x SST SAMSAS requests only.
4 mL
FISH - FLUORESCENCE IN SITU HYBRIDISATION - BLOOD
1 x Lithium Heparin
Please confirm with Genetics Dept. on 08 9317 0922 if unsure about FISH request
2mL
FISH -FLUORESCENCE IN SITU HYBRIDISATION- PRENATAL
DOCTOR COLLECT
FISH FOR H.E.S 1 x EDTA - dedicated tube required and 1 x Lithium Heparin - DO NOT SPIN
Referred to: PathWest FSH Lithium Heparin sample - Place DO NOT SPIN sticker around the lid.
3mL each
FLAVIVIRUS SEROLOGY 1 x SST Referred to: PathWest QEII 2 mL
FLECAINIDE 1 x Plain Tube (Red Top) - Spin, Separate & Freeze
Referred to: QML Pathology 2 mL
FLOW CYTOMETRY 1 x ACD and 1 x EDTA
Full tube of ACD (can use 1mL of EDTA ONLY if paediatric or elderly/infirm). See: WI-COLL-96 - Flow Cytometry
6mL ACD
FLUCLOXACILLIN 1 x Lithium Heparin spin, separate & freeze ASAP
Referred to: PathWest FSH 2 mL
FLUCONAZOLE 1 x EDTA - dedicated tube required, spin, separate & freeze ASAP
Referred to: SydPath Pathology at St Vincent's Hospital (NSW) Transport on ice brick. Do not use tubes containing separator gel.
1 mL
Specimen Collection Manual Issue 19 Page 38 of 95
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Vol
FLUCYTOSINE 1 x EDTA - spin, separate & freeze
Referred to: SydPath Pathology at St Vincent's Hospital (NSW) Collect just prior to next dose. Provide clinical and medication details.
3mL
FLUNITRAZEPAM 1 x Lithium Heparin - Spin, Separate, Foil & Freeze ASAP
Referred to: QML Pathology Collect immediately prior to next dose. Provide clinical and medication details, including time and date of last dose
2mL
FLUORIDE 1 x Urine Container
Referred to: PathWest QEII Keep urine cold on ice brick.
10 mL
FLUOXETINE 1 x Plain Tube (Red Top) - Spin, Separate & Freeze ASAP
Referred to: Royal Prince Alfred (NSW) Trough level required. Provide clinical and medication details, including time and date of last dose. Transport sample on ice brick.
2mL
FNA CYTOLOGY Yes DR COLLECT If a WDP Pathologist is required to perform the FNA procedure, advise patient to contact 08 9317 0916 on Monday to Friday between 9am-5pm to make a booking. NOTE: Pathologist performed FNAs are only done at the Mount Hospital Laboratory Collection Centre. No out of pocket expense if patient is Pensioner or Veterans Affairs.
FOETO-MATERNAL TEST 1 x EDTA 6mL - Pink, dedicated tube
1. Full Name, DOB or URN, DOC & TOC must be on EDTA tube and request form. All information must match between form and sample.
2. Collector must sign the tube and certifier on form - these signatures must match.
Contact Transfusion Medicine Dept. on 08 9321 3300 for any enquiries.
1mL
FOLATE 1 x SST 2mL
FOLIC ACID 1 x SST 2mL
FOLLICLE STIMULATING HORMONE (FSH)
1 x SST 2mL
FRAGILE X Yes 1 x EDTA - dedicated tube required
Referred to: Genomic Diagnostics It can also be part of the Genetic Carrier Screen (for females).
1mL
FREE ANDROGEN INDEX (FAI) 1 x SST 1mL
Specimen Collection Manual Issue 19 Page 39 of 95
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Vol
FREE FATTY ACID 1 x SST spin separate and freeze
Referred to: PathWest QEII NOTE: This is not ESSENTIAL FATTY ACIDS or VERY LONG-CHAIN FATTY ACIDS.
2mL
FREE LIGHT CHAINS 1 x SST 3mL
FREE PSA 1 x SST Patient to complete FRM-COLL-36 Prostate Specific Antigen (PSA) Patient Questionnaire
2mL
FREE TESTOSTERONE 1 x SST 3mL
FRISIUM 1 x Plain Tube (Red Top) - Spin, Separate & Freeze
Referred to: Royal Brisbane and Women’s Hospital (QLD) Record dosage and time and date of last dose.
2mL
FRUCTOSAMINE 1 x SST Referred to: QML Pathology Provide clinical and medication details.
2mL
FRUSEMIDE 1 x Urine Container - random
Referred to: Royal Melbourne Hospital 20 mL
FSH 1 x SST 2mL
FT3 1 x SST Patient to complete FRM-COLL-45 Patient questionnaire for Thyroid Function Tests
2mL
FT4 1 x SST Patient to complete FRM-COLL-45 - Patient questionnaire for Thyroid Function Tests
2mL
FULL BLOOD COUNT 1 x EDTA Full Name, DOB or URN, DOC & TOC must be on EDTA tube. Collector must sign EDTA tube and request form. NOTE: Only 1 EDTA required for any combination of FBC/Indices, ESR, Malaria, RET, HbA1C, HE. Occasionally Dr will request citrate for platelet count. Collect 1 x citrate and 1 x EDTA.
2ml
FUNGAL ANTIBODIES 1 x SST Referred to: PathWest QEII 2 mL
G6PD 1 x EDTA - dedicated tube required
Referred to: QML Pathology or PathWest QEII (child samples or commercial) NOTE: G6PD must be analysed within 5 days of sample collection.
1 mL
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Vol
GABAPENTIN 1 x Plain Tube (Red Top) - Spin, Separate & Freeze
Referred to: Royal Prince Alfred Hospital (NSW) Provide clinical and medication details including date and time of last dose. Collect trough sample just prior to next dose.
1mL
GAD 1 x SST Referred to: QML Pathology Test can be sent to PathWest if Islet cell is also requested - testing: Fortnightly on Mondays.
1mL
GALACTOMANNAN ANTIGEN 1 x SST Referred to: PathWest FSH 2mL
GASTRIN 1 x Lithium Heparin spin, separate & freeze ASAP
Referred to: PathWest FSH 1mL
GAUCHER DISEASE Yes 1 x EDTA 6mL - Pink, dedicated tube
Referred to: Women's and Children's Hospital (SA) Advise patient there will be out of pocket (estimated cost: $2550.00 – subject to change). If the patient does not accept the cost, do not collect a specimen for the test and advise the patient to discuss with the requesting doctor. Do not collect on Fridays. Women's and Children's Hospital, Adelaide needs to receive sample within 48 hours.
6 mL
GBM 1 x SST Referred to: PathWest QEII Tested on Friday AM
2mL
GCT 1 x Fluoride Oxalate
Patient does not need to be fasting. 1. Give 50g drink 2. 1-hour post drink, take 1 FLOX See: WI-COLL-57 - Gestational Diabetes Test
2mL
GENERAL SWABS/FLUIDS MC&S Swab - Blue Top Gel or Urine Container
DO NOT REFRIGERATE Based on Request see Eye swab WI, Nasal swab WI, Sputum culture WI, Throat swab WI, Wound swab WI,
Specimen Collection Manual Issue 19 Page 41 of 95
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Vol
GENERATION NON INVASIVE PRENATAL TESTING (NIPT)
Yes 1 x Streck tube Referred to: Genomic Diagnostics Collection Method: See: WI-COLL-79 Generation NIPT Blood Collection Collection Window: Generation (test code: NIP) collections all days. Generation Plus (test code: NPX) (Perth Metro): Monday & Tuesday before 11am. Generation Plus (test code: NPX) (Regional & NT): Monday only before 11am. Payment: Call Genomic Diagnostics 1800 822 999 (Mon-Fri 9am-5pm AEST) for payment. Online payments also available: genomicdiagnostics.com.au/shop/generation-nipt/ Attach FRM-COLL-87 NIPT Pre-payment Authorisation Form if patient has not made payment prior to attending the collection centre. Request form: Must have NIPT request form: Generation or competitor NIPT request form (regular request forms not accepted). Enquiries to WDP Genetics Dept. on 08 9317 0922 or Genomic Diagnostics 1800 822 999 (Mon-Fri 9am-5pm AEST)
7mL
GENETIC CARRIER SCREENING Yes 2 x EDTA - dedicated tube required
Can collect one EDTA when all three tests (CF, SMA and FraX) are requested together. Performed at Genomic Diagnostics Combination testing for Cystic Fibrosis, Spinal Muscular Atrophy and Fragile X
Specimen Collection Manual Issue 19 Page 42 of 95
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Vol
GENITAL MICRO /MC&S DOCTOR COLLECT DO NOT REFRIGERATE Please Note swabs collected from genital sites are required to be collected by referring doctor.
GENTAMICIN (URGENT – Result before next dosing)
1 x Plain Tube (Red Top)
Result required before next dosing. Test is performed at Joondalup Laboratory. Record, time, date, amount of last dose and next dose. For regional and NT collection centres Please refer the sample to the nearest hospital
1ml
GESTATIONAL DIABETES SCREEN 1 x Fluoride Oxalate
Patient does not need to be fasting. 1. Give 50g drink 2. 1-hour post drink, take 1 x FLOX See: WI-COLL-57 - Gestational Diabetes Test
2mL
GGT 1 x SST 2mL
GGT (FLUID) DOCTOR COLLECT 2mL
GILBERT’S SYNDROME Yes 1 x EDTA - dedicated tube required DO NOT SPIN
Referred to: PathWest QEII DO NOT SPIN down, separate or freeze sample. Optimal DNA yield is extracted from a sample within 3 days of sample collection. DNA degradation can occur in samples older than 3 days post collection. This degradation is significant after 5 days post collection.
1mL
GLIADIN ANTIBODIES 1 x SST 1ml
GLOBULINS (CALCULATED) 1 x SST 2mL
GLOMERULAR FILTRATION RATE 1 x SST 2mL
GLUCAGON 1 x Special Collection Tube – Spin, separate and freeze ASAP
Referred to: Royal Prince Alfred Hospital (NSW) Patient must be fasting. Collection at Myaree Laboratory ONLY Special Tube (0.25mL TRAYSOL in 5mL EDTA) is available from Manual Chemistry (08 9317 0838)
2 mL
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Vol
GLUCOCORTICOSTEROID 1 x SST AM sample: to be collected between 0800 - 0900 am. PM sample: to be collected between 1600 - 1700 pm. Random sample: anytime and note the time of collection on the form.
2mL
GLUCOSE (ACC) 1 x SST Serum must be spun within 4 hours of collection. No exception. If unable to spin on site, collect a FLOX.
2mL
GLUCOSE (DOCTOR/NURSING HOME/MOBILE COLLECT)
1 x Fluoride Oxalate
2mL
GLUCOSE (FLUID) DOCTOR COLLECT 2mL
GLUCOSE (POST PRANDIAL) 1 x Fluoride Oxalate
Post prandial means after a meal. Glucose sample to be collected at a certain time post prandial (doctor will specify time). 1-hour Post Prandial PP - collect 1 x FLOX 1 hour after meal. 2-hour Post Prandial PP - collect 1 x FLOX 2 hour after meal. When noting the time of the last meal on the request form - state the time to the nearest half hour. See: WI-COLL-59 - Glucose Post Prandial Testing
2mL
GLUCOSE CHALLENGE TEST 1 x Fluoride Oxalate
Patient does not need to be fasting. 1. Give 50g drink 2. 1-hour post drink, take 1x FLOX See: WI-COLL-57 - Gestational Diabetes Test
2mL
GLUCOSE TOLERANCE TEST 3 x Fluoride Oxalate
Note if GROWTH HORMONE is co-requested (refer to GROWTH HORMONE SUPPRESSION TEST). Note if INSULIN co-requested (refer to INSULIN LEVELS). See: WI-COLL-58 - Glucose Tolerance Testing
2mL
GLUTATHIONE PEROXIDASE 1 x Lithium Heparin - DO NOT SPIN
Referred to: Royal Brisbane Hospital Transport on ice brick
1 mL
GLYCEROL 1 x Urine Container - random
Referred to: Royal Brisbane Hospital 10mL
Specimen Collection Manual Issue 19 Page 44 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
GNATHOSTOMA 1 x SST Referred to: PathWest
GOLD 2 x EDTA - dedicated tube required
Referred to: Royal Prince Alfred Collect immediately prior to next dose or at least 8 hours after last dose. Provide clinical and medication details.
0.5 mL
GONADOTROPHINS 1 x SST 2mL
GROUP AND HOLD (G&H) (URGENT)
1 x EDTA 6mL - Pink, dedicated tube
1. Ask the patient whether they have been transfused or pregnant in the last 3 months – note on request form.
2. Ask date of operation and which hospital it is to be performed at – note on request form.
3. Full Name, DOB or URN, DOC & TOC must be on EDTA tube and request form. All information must match between form and sample.
4. Collector must sign the tube and certifier on form - these signatures must match.
Contact Transfusion Medicine Dept. on 08 9321 3300 for any enquiries.
6mL
GROWTH HORMONE 1 x SST - Spin and freeze ASAP
Growth Hormone and IGF-1 are usually co-requested but can be requested individually. If tests are co-requested, only collect 1x SST spin, separate and freeze ASAP for both tests. Patient should be fasting unless specifically requested. Allow patient to rest (sitting or lying down) for 30 minutes prior to collection. Note RESTED on request form. If patient is reluctant/ unable to wait, then collect the sample and note NOT RESTED on request form.
4mL
GROWTH HORMONE SUPRESSION TEST
5 x SST - Spin and freeze and 5 x Fluoride Oxalate
1. Collect fasting SST and FLOX. 2. Give patient 75g glucose drink. 3. Collect SST and FLOX at 30 min, 60
min, 90 min and 120 min post glucose drink.
1 mL each
Specimen Collection Manual Issue 19 Page 45 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
GTT EXTENDED 5 x Fluoride Oxalate
1. Collect 1 x fasting, 2. Give patient 75g glucose drink 3. Collect 1 x 1-hour post drink, 4. Collect 1 x 2-hour post drink, 5. Collect 1 x 3-hour post drink and 6. Collect 1 x 4-hour post drink. See: WI- COLL-58 - Glucose Tolerance Testing
2mL each
GUMP 1 x Dry Swab - Orange/White
Referred to: PathWest QEII See WI-COLL-115 and FRM-COLL-81 (Swab Collection guide)
H5N1 1 x SST Referred to: PathWest QEII 1mL
HAEMOCHROMATOSIS GENE (HFE)
Yes 1 x EDTA - dedicated tube required
Referred to: Genomic Diagnostics Eligible for Medicare rebate if patient has elevated transferrin saturation or ferritin on repeat testing or patient has a first degree relative with haemochromatosis or first degree relative with homozygosity for C282Y or compound heterozygote C282Y/H63D. If unsure, advise patient to contact Genomic Diagnostics 1800 822 999 (Mon-Fri 9am-5pm AEST)
6mL
HAEMOGLOBIN A1C 1 x EDTA Full Name, DOB or URN, DOC & TOC must be on EDTA tube. Collector must sign EDTA tube and request form. Samples from distant collection sites should be kept as cool as possible post collection, and in transit, until it reaches the laboratory. NOTE: Only 1 EDTA required for any combination of FBC/Indices, ESR, Malaria, RET, HbA1C, HE.
1ml
HAEMOGLOBIN/THALASSAEMIA DNA STUDIES
1 x EDTA and 1 x SST
Samples from distant collection sites should be kept as cool as possible post collection, and in transit, until it reaches the laboratory. Only performed as an additional test dependent on Hb Electrophoresis (HE) results. Sample will be referred by Haematology staff if required.
2 mL each
Specimen Collection Manual Issue 19 Page 46 of 95
Test Name Out of Pocket
Specimen
Collection Method Min Vol
HAEMOGLOBINOPATHY STUDIES 1 x EDTA and 1 x SST
Full Name, DOB or URN, DOC & TOC must be on EDTA tube. Collector must sign EDTA tube and request form. Sample coming from a distant collection centre must be kept as cool as possible post collection, and in transit, until it reaches the laboratory. NOTE: Only 1 EDTA required for any combination of FBC/Indices, ESR, Malaria, RET, HbA1C, HE. FBC and IRS need entering along with HE - All required for interpretation.
2ml each
HAEMOPHILIA GENE TEST Yes 1 x EDTA 6mL - Pink, dedicated tube
Referred to: Adelaide Women's and Children's Hospital. If abnormal Factor VIII price will increase. Consent is required before sending. Factor VIII result is also required to be sent with samples and consent.
4mL
HAEMOPHILIA SCREEN See FACTOR STUDIES entry for Collection requirements. See: FRM- COLL-86 - Coagulation Collection Guide
HAEMOPHILUS DUCREYI PCR 1 x Dry Swab - Orange/White
Referred to: PathWest QEII See WI-COLL-115 and FRM-COLL-81 (Swab Collection guide)
HAEMOPHILUS INFLUENZAE B AB 1 x SST Referred to: Children's Hospital at Westmead (NSW)
2 mL
HAIR ANALYSIS DOCTOR COLLECT
HALOPERIDOL 1 x Plain Tube (Red Top)
Referred to: PathWest QEII 1 mL
HBA1C 1 x EDTA Full Name, DOB or URN, DOC & TOC must be on EDTA tube. Collector must sign EDTA tube and request form. Samples from distant collection sites should be kept as cool as possible post collection, and in transit, until it reaches the laboratory. NOTE: Only 1 EDTA required for any combination of FBC/Indices, ESR, Malaria, RET, HbA1C, HE.
1ml
HCG 1 x SST 2mL
HDL 1 x SST See: WI-COLL-61 - Lipid Testing 2mL
HE4 Yes 1 x SST Referred to: Laverty Pathology. Spin & transport on ice brick.
1 mL
HEATSHOCK IGAM 1 x SST spin separate and freeze
Referred to: ICPMR (NSW) 1 mL
Specimen Collection Manual Issue 19 Page 47 of 95
Test Name Out of Pocket Specimen Collection Meth00od Min
Vol
HEAVY METAL SCREEN 1 x Trace Element Free - DO NOT SPIN
Referred to: Laverty Pathology 4mL
HELICOBACTER ANTIBODIES 1 x SST 2mL
HELICOBACTER BREATH TEST 1 x PYTest kit (C- 14 Urea Breath Test)
Do not do the test if the patient is pregnant or if the patient is likely to be pregnant or breastfeeding. See WI- COLL-93 - Helicobacter C14 collection and FRM-COLL-90 - Helicobacter Restricted Medication List
HELICOBACTER PYLORI ANTIGEN STOOLS
1 x Faeces Container
HELICOBACTER PYLORI SEROLOGY 1 x SST 2 mL
HEP B VIRAL LOAD 1 x SST - dedicated tube required - DO NOT OPEN
Referred to: QML Pathology Must be a pristine SST tube – place DO NOT OPEN sticker around the lid.
1mL
HEPARIN-INDUCED THROMBOCYTOPENIA SCREENING
4 x Sodium Citrate and 1 x Plain Tube (Red Top)
Referred to: PathWest FSH Must be at FSH within 4hrs of collection (or can be spun/frozen then sent). Contact PathWest prior to collection. Must submit a vial of the same Heparin given to the patient. See: FRM-COLL-86 - Coagulation Collection Guide
10 mL
HEPASCORE Yes 1 x SST Referred to: PathWest QEII 2 mL
HEPATITIS A IGG ANTIBODY 1 x SST 2 mL
HEPATITIS A IGM ANTIBODY 1 x SST 2 mL
HEPATITIS B CORE ANTIBODY 1 x SST 2 mL
HEPATITIS B E ANTIGEN / ANTIBODY
1 x SST 2 mL
HEPATITIS B SURFACE ANTIBODY 1 x SST 2 mL
HEPATITIS B SURFACE ANTIGEN 1 x SST 2 mL
HEPATITIS C GENOTYPE 1 x SST - dedicated tube required
Referred to: PathWest FSH This test uses significant serum volume- please ensure full SST is collected. Only sent away if checked by a Microbiologist.
8 mL
HEPATITIS C PCR 1 x SST - dedicated tube required
Referred to: PathWest FSH This test uses significant serum volume- please ensure a full SST is collected. Only sent away if checked by a Microbiologist.
8 mL
Specimen Collection Manual Issue 19 Page 48 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
HEPATITIS C VIRAL LOAD 1 x SST - dedicated tube required - DO NOT OPEN
Referred to: PathWest FSH Must be a pristine SST tube – place DO NOT OPEN sticker around the lid.
8 mL
HEPATITIS C VIRUS ANTIBODY 1 x SST 2 mL
HERBICIDES Yes 1 x Urine Container - random
Referred to: WorkCover NSW If PESTICIDES are also requested, collect blood (see PESTICIDES).
20 mL
HEREDITARY NEUROPATHY DNA TEST
2 x EDTA 6mL - Pink, dedicated tube
Referred to: PathWest QEII Specimens are received at PathWest Monday to Friday 0800 to 1700. Prenatal diagnosis must be arranged prior - please contact PathWest QEII well in advance of sample collection date.
2 mL each
HEREDITARY SPASTIC PARAPLEGIA 2 x EDTA 6mL - Pink, dedicated tube
Referred to: PathWest QEII. Specimens are received at PathWest Monday to Friday 0800 to 1700. Requests for predictive testing should be directed through Genetic Services of WA or the Neurosciences Unit. Prenatal diagnosis must be arranged prior - please contact PathWest QEII laboratory well in advance of sample collection date.
2 mL each
HEREDITARY SPHEROCYTOSIS 1 x EDTA - dedicated tube required
Referred to: PathWest QEII Transport on ice brick - to arrive in testing laboratory within 48 hours of collection (Collect between Mon-Thursday).
1mL
HERPES SEROLOGY 1 x SST 1 mL
HERPES SIMPLEX PCR 1 x Dry Swab - Orange/White or 1 x Swab - Viral Transport Media
See WI-COLL-115 and FRM-COLL-81 (Swab Collection guide) Please note swabs collected from genital sites are required to be collected by the referring doctor. PathWest can run PCR off Whole EDTA but not recommended as may contain PCR inhibitors.
HETEROPHILE ANTIBODIES 1 x SST 2mL
HFE- SEE HEAMOCHROMATOSIS
HGPRT 1 x Lithium Heparin - DO NOT SPIN
Referred to: Royal Brisbane Hospital 5 mL
HHV6 PCR 1 x EDTA - dedicated tube required
Referred to: PathWest QEII 2mL
Specimen Collection Manual Issue 19 Page 49 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
HHV6 SEROLOGY 1 x SST Referred to: PathWest QEII 1mL
HHV8 PCR 1 x EDTA - dedicated tube required
Referred to: PathWest QEII 2mL
HIGH DENSITY LIPOPROTEIN 1 x SST See: WI-COLL-61 - Lipid Testing 2mL
HIGH SENSITIVE TROPONIN (URGENT – Result within 2hours)
1 x SST Must be in the testing lab within 1 hour – call Laboratory Liaising Officer on 08 9317 0814 for Myaree. For country and NT laboratories: call the testing laboratory directly. Collectors to obtain an after-hours contact number for requesting doctors.
2mL
HISTAMINE BLOOD Yes 2 x Lithium Heparin frozen whole - DO NOT SPIN
Referred to: Sullivan Nicolaides (QLD) Provide clinical and medication details.
4mL each
HISTOLOGY Formalin pot Dr collect only.
HISTONE ANTIBODIES 1 x SST Referred to: PathWest QEII Only if ANA is positive.
1mL
HISTOPLASMOSIS SEROLOGY SST Referred to: PathWest QEII 2 mL
HISTOPLASMOSIS URINE 1 x Urine Container - random
Referred to: PathWest QEII 10 mL
HITT OR HIT SCREEN See HEPARIN-INDUCED THROMBOCYTOPENIA SCREENING entry for collection details. See: FRM-COLL-86 - Coagulation Collection Guide
HIV RESISTANCE TESTING 2 x EDTA - spin, separate & freeze ASAP
Referred to: PathWest FSH Transport on ice brick. Can be performed alongside HIV viral load
4 mL
HIV SEROLOGY (NOT VIRAL LOAD) 1 x SST NOTE: This is not HIV VIRAL LOAD If HIV Viral Load is requested, collect 2 x EDTA spin, separate and freeze.
2 mL
HIV VIRAL LOAD 2 x EDTA - spin, separate & freeze ASAP
Referred to: PathWest FSH Transport on ice brick.
4 mL
HIV WESTERN BLOT Yes SST Only to be added by the Lab
Specimen Collection Manual Issue 19 Page 50 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
HLA ANTIBODIES SST Referred to: PathWest FSH
HLA B 1502 ACD & EDTA Referred to: PathWest FSH
HLA DQB1 0620 2 x ACD Referred to: PathWest FSH
HLA TYPING ACD Referred to: PathWest FSH
HLA-B27 1 x EDTA 6mL - Pink
Referred to: Genomic Diagnostics 6mL
HLA-DR FOR RA MOTIFS EDTA or ACD Referred to: PathWest QEII 9mL
HMG - COA REDUCTASE AB SST Referred to: PathWest QEII
HOLTER MONITOR Holter Monitor Done at specific sites only by phone appointment. See FRM-COLL-102 - Specialised Test Locations
HOMOCYSTEINE 1 x PPT spin within 30 minutes of collection
Spin within 30 minutes of collection. Do not freeze or separate.
2mL
HOMOCYSTEINE URINE 1 x Urine Container - dedicated jar required. Freeze ASAP
Referred to: PathWest QEII 20mL
HORMONE PROFILE 1 x SST 2mL
HRP 1 x SST 2mL
HSV PCR (PAEDIATRIC BLOOD) 1 x EDTA This test is for requests that specifically request HSV PCR from blood on paediatric patients only. For all other Herpes simplex PCR see HSV PCR. If collected out of hours, the on- call Clinical microbiologist must be contacted.
1mL
HTLV SEROLOGY 1 x SST Referred to: PathWest QEII 2mL
HUMIRA 1 x SST spin separate and freeze ASAP
Referred to: PathWest QEII Collect trough level
HUNTINGTON'S STUDIES 2 x EDTA 6mL - Pink, dedicated tube
Referred to: PathWest QEII Ideally two separate specimen collection are required on two separate days. Please collect 1 x EDTA 6mL then provide DT form to patient for second collection for the following day on a separate lab number.
6mL
HYDATID (HA) SEROLOGY 1 x SST Referred to: PathWest QEII
Specimen Collection Manual Issue 19 Page 51 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
HYDROGEN/ METHANE BREATH TEST
Yes Special Collection Kit
Home collect or book appointment through Gastrolab on 1300 624 771. Gastrolab is located at Mt Pleasant ACC (Unit 6 16 Queens Rd). Patient can either visit Gastrolab for sample collection or can be ordered from home by ordering collection kit from www.wdp.com.au.
HYDROXY PHENOL ACETIC ACID 1 x Urine Container - random
Referred to: PathWest QEII 20mL
HYDROXYCHLOROQUINE 1 x Lithium Heparin - DO NOT SPIN
Referred to: PathWest QEII Peak and trough samples collected (i.e. pre and 2-hour post tablet). Place DO NOT SPIN sticker around the lid.
1mL
HYDROXYCHOLECALCIFEROL Yes 1 x SST Western Diagnostic Pathology will bulk bill when indicated by referring doctor. WDP assume requesting doctor has determined that patient meets requirements as stipulated in the Medicare Benefits Schedule (Item No: 66833) Request form to be stamped with "Patient Advised of Fee" and signed by the patient acknowledging that an out of pocket payment will be due. See: WI-COLL- 113 Vitamin D Bulk Billing Criteria
2mL
IA2 ANTIBODIES 1 x SST Referred to: PathWest QEII 2mL
IDH 1 & 2 1 x EDTA 6mL - Pink, dedicated tube
For patients with AML 2mL
IGA 1 x SST 2mL
IGA SUBCLASSES DO NOT COLLECT - test not currently available in Australia
IGD 1 x SST Referred to: Royal Prince Alfred Hospital
2mL
IGE 1 x SST 2mL
IGF 2 Test Not Available 2mL
IGF BP3 1 x SST spin separate and freeze
Referred to: PathWest QEII 1mL
IGF-1 1 x SST - Spin and freeze
Spin and freeze IGF-1 and Growth Hormone are usually co-requested but can be requested individually. If tests are co-requested, only collect 1 SST (spin and freeze) for both tests.
4mL
Specimen Collection Manual Issue 19 Page 52 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
IGG 1 x SST 2mL
IGG FOOD SENSITIVITY 1 x SST Referred to: Healthscope Functional Health Pathology
2mL
IGG SUBCLASSES 1 x SST Referred to: QML Pathology 2mL
IGM 1 x SST 2mL
IMMUNOFIXATION 1 x SST If the urine immunofixation is requested, collect 1x spot urine or 24-hour urine if requested by doctor.
2 mL
IMMUNOGLOBULIN D 1 x SST Referred to: Royal Prince Alfred Hospital
2mL
IMMUNOGLOBULINS 1 x SST 2mL
IMMUNOPHENOTYPING 1 x ACD and 1 x EDTA
Full tube of ACD (can use 1mL of EDTA ONLY if paediatric or elderly/infirm). See: WI-COLL-96 - Flow Cytometry
6mL ACD
INFECTIOUS MONONUCLEOSIS SCREEN
1 x SST 2 ml
INFLUENZA A&B 1 x SST Referred to: QML Pathology 2mL
INFLUENZA A/B PCR 1 x Dry Swab - Orange/White or 1 x Swab - Viral Transport Media
See WI-COLL-115 and FRM-COLL-81 (Swab Collection guide)
INHIBIN A/B/ SCREEN Yes 1 x SST spin separate and freeze
Referred to: QML Pathology 1mL
INHIBITOR SCREEN
3 x Sodium Citrate
To Myaree within 4hrs (transport in blue bag) or single spin/separate/freeze. Transport on dry ice. Patient to complete Coagulation Questionnaire: FRM- COLL-43. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
INORGANIC PHOSPHATE 1 x SST 2mL
INR OR INTERNATIONAL NORMALISED RATIO (WARFARIN MONITORING)
1 x Sodium Citrate Must be at the lab within 24 hours (transport in blue bag) or single spin, separate and freeze. For patients on WARFARIN (Coumadin or Marevan). Record date, time and amount of last dose on form. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
INSECTS IDENTIFICATION 1 x Urine Container
Referred to: Dr Peter Neville | Medical Entomologist Medical Entomology | Public Health Division Mount Claremont. Normally submitted by patient in container already.
Specimen Collection Manual Issue 19 Page 53 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
INSTANT DRUG SCREEN Full Chain of Custody Collect Please ensure you have completed all relevant fields on the chain of custody before the donor leaves. All non- negative cups need to be split into Vacutainers and sent into the laboratory for storage. Please fax completed form to Toxicology Dept. on 08 93170859 for resulting. See: WI-COLL- 53 - Drugs of Abuse Collection Manual
10mL
INSULIN 1 x SST Patient should be fasting unless specifically indicated
2 mL
INSULIN ANTIBODIES 1 x SST spin separate and freeze
Referred to: Royal Prince Alfred Hospital
1mL
INSULIN LEVELS 3 x SST and 3 x Fluoride Oxalate
Patient MUST be fasting. 1. Collect 1 x fasting SST & 1x FLOX. 2. Give 75g glucose drink 3. Collect 1x SST & 1x FLOX 1-hour
post drink 4. Collect 1x SST & 1x FLOX 2-hour
post drink
2 mL each
INTERLEUKIN 28B POLYMORPHISM GENOTYPE (IL28B)
1 x EDTA - dedicated tube required
Referred to: PathWest QEII 1mL
INTERLEUKIN 6 Yes 1 x SST - spin separate and freeze
Referred to: QML Pathology 1mL
INTRINSIC FACTOR ANTIBODY 1 x SST 1mL
IODINE BLOOD Yes 1 x Trace Element Free - DO NOT SPIN
Referred to: Royal North Shore (NSW) Place DO NOT SPIN sticker around the lid.
2mL
IODINE URINE Yes 1 x Urine Container - random
Referred to: Laverty Pathology 20mL
IONISED CALCIUM 1 x SST - dedicated tube required – DO NOT OPEN
Must be a pristine SST tube – place DO NOT OPEN sticker around the lid. If recollect is required for CAI, check if PTH is also requested. If yes, PPT must be collected at the same time for PTH (VDU: CAI, PTH). Do not use torniquet for calcium collection.
4 mL
IRON STUDIES 1 x SST 2mL
ISLET CELL ANTIBODIES 1 x SST Referred to: PathWest QEII 1mL
ISOAMYLASE Test Not Available
Specimen Collection Manual Issue 19 Page 54 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
ISOCYANATES URINE Yes 1 x Urine Container - random
Referred to: WorkCover NSW End of shift. Urine collection must be made within 48hrs of last exposure. Keep samples cool.
50mL
JAK 2 MUTATION Yes 2 x EDTA 6mL - Pink, dedicated tube
Referred to: Laverty Pathology When asked with JAK 2 Mutation and/or MPL Mutation only 2x EDTA 6ml Pink required altogether.
1mL
JAPANESE B ENCEPHALITIS VIRUS 1 x SST Referred to: PathWest QEII 2mL
JOINT FLUID CYTOLOGY DR COLLECT
KARYOTYPE - SEE CHROMOSOMES
KEPPRA 1 x Plain Tube (Red Top) - Spin & Separate
Referred to: Sullivan Nicolaides Provide clinical and medication details. Post-Dose: 1 hour after oral dose. Trough-Dose: 8 hours after oral dose.
2mL
KETONES SERUM 1 x Lithium Heparin - DO NOT SPIN
Referred to: PathWest QEII Sample MUST be kept cold immediately after collection. Transport on ice brick.
1mL
KETONES URINE 1 x Urine Container - random
Urine sample must be fresh. 1mL
KETOSTEROIDS URINE 1 x Urine Container- 24hr plain
Referred to: Dorevitch Pathology Children acceptable for 6- 12HRS early morning collection.
KFT 1 x SST Spin within 4 hours. Do not transport on an ice brick.
2mL
KIT GENE FOR MASTOCYTOSIS Yes 1 x EDTA - dedicated tube required
Referred to: Molecular Haematology, Peter MacCallum Cancer Centre, Victorian Comprehensive Cancer Centre Building, 305 Grattan St Melbourne 3000 VICTORIA
5mL
KLEIHAUER TEST 1 x EDTA 6mL - Pink, dedicated tube
1. Full Name, DOB or URN, DOC & TOC must be on EDTA tube and request form. All information must match between form and sample.
2. Collector must sign the tube and certifier on form - these signatures must match.
Contact Transfusion Medicine Dept. on 08 9321 3300 for any enquiries.
2 mL
Specimen Collection Manual Issue 19 Page 55 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
KLEIHAUER TEST (KL) / FOETO- MATERNAL HAEMORRHAGE
1 x EDTA - dedicated tube required
1. Full Name, DOB or URN, DOC & TOC must be on EDTA tube and request form. All information must match between form and sample.
2. Collector must sign the tube and certifier on form - these signatures must match.
If unsure what to collect ring the Transfusion Medicine Dept. on 08 9321 3300
1mL
KLINEFELTER SYNDROME 1 x Lithium Heparin
Referred to: QML Pathology 1.5mL
KUNJIN SERO 1 x SST Referred to: PathWest QEII 2mL
LACTATE 1 x Fluoride Oxalate
LACTATE DEHYDROGENASE 1 x SST 2mL
LACTATE PYRUVATE Special Tube (no gel) with Special Preservative - DO NOT SPIN and freeze ASAP
Referred to: PathWest QEII Contact Manual Chemistry on 08 9317 0838 to order the special tube 1. Add blood to line (1 mL) to special
tube 2. Mix well 3. DO NOT SPIN OR SEPARATE 4. Sample will turn into brown
colour 5. Freeze ASAP
Contact the team leader for any collection queries
1mL
LACTOSE INTOLERANCE TEST 4 x Fluoride Oxalate
Contact Myaree patient reception (08 9317 0704) for lactose powder. Collect: 1. 1 x fasting FLOX 2. Give lactose drink 3. 1 x FLOX 30 minutes post drink 4. 1 x FLOX 1-hour post drink 5. 1 x FLOX 2 hours post drink See: WI-COLL-60 - Lactose Tolerance Testing
2mL
Specimen Collection Manual Issue 19 Page 56 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
LACTOSE TOLERANCE TEST 4 x Fluoride Oxalate
Contact Myaree patient reception (08 9317 0704) for lactose powder. Collect: 1. 1 x fasting FLOX 2. Give lactose drink 3. 1 x FLOX 30 minutes post drink 4. 1 x FLOX 1-hour post drink 5. 1 x FLOX 2 hours post drink See: WI-COLL-60 - Lactose Tolerance Testing
2mL
LAMOTRIGINE 1 x Plain Tube (Red Top)
Referred to: QML Pathology Provide clinical and medication details, including time, date and amount of last dose. Collect sample immediately prior to next dose.
2mL
LANOXIN 1 x SST Record date and time of last dose of medication on the form. Bloods should be taken 6 hours post dose. Do not turn patients away if not 6-hour post dose.
2mL
LC-1 ANTIBODIES 1 x SST Referred to: PathWest QEII 1mL
LDH ISOENZYMES 1 x SST Referred to: Royal Prince Alfred Hospital Sample must be room temperature. Only send if LDH is elevated.
2mL
LDL SUBFRACTIONS Yes 1 x SST - dedicated tube required
Referred to: San Pathology Sydney Adventist Hospital Provide clinical & medication details. Spin sample and transport on ice brick.
2mL
LEAD BLOOD 1 x Trace Element Free - DO NOT SPIN
Referred to: Laverty Pathology Place DO NOT SPIN sticker around the lid.
6mL
LEAD URINE 1 x Urine Container
Referred to: Laverty Pathology Post shift collect.
20mL
Specimen Collection Manual Issue 19 Page 57 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
LEFLUNOMIDE Yes 1 x Lithium Heparin
Referred to: Royal Brisbane Hospital
LEGIONELLA PCR 1 x Dry Swab - Orange/White or 1 x Swab - Viral Transport Media
See WI-COLL-115 and FRM-COLL-81 Note: This test is only performed on specific request for respiratory Legionella pneumophila and Legionella longbeachae
LEGIONELLA SEROLOGY 1 x SST Referred to: QML Pathology 2mL
LEGIONELLA URINARY ANTIGEN 1 x Urine Container - random
Referred to: PathWest QEII 20mL
LEISHMANIASIS SEROLOGY 1 x SST Referred to: ICPMR Westmead (NSW) 2mL
LEPTIN Yes 1 x SST spin separate and freeze
Referred to: Royal Prince Alfred Hospital
5mL
LEPTOSPIROSIS 1 x SST Referred to: QML Pathology 2mL
LEPTOSPOROSIS PCR 1 x EDTA - dedicated tube required
Referred to: PathWest QEII 2mL
LFT 1 x SST 2mL
LH 1 x SST 2mL
LIPASE 1 x SST 2mL
LIPID STUDIES 1 x SST See: WI-COLL-61 - Lipid Testing 2mL
LIPIDS 1 x SST See: WI-COLL-61 - Lipid Testing 2mL
LIPOPROTEIN A 1 x SST Referred to: PathWest FSH 2mL
LIPSASE (FLUID) DOCTOR COLLECT 2mL
LITHIUM 1 x SST Record: date, time of last dose and the dosage on the form.
2mL
LKM 1 x SST Please refer to TPO if THYROID MICROSOMAL AB is requested.
1mL
LUPUS ANTICOAGULANT 2 x Sodium Citrate To Myaree within 10hrs - transport in blue bag. Or DOUBLE spin/separate/freeze. Patient to complete questionnaire FRM-COLL- 43. See: FRM-COLL-86 - Coagulation Collection Guide
2.7 mL
Specimen Collection Manual Issue 19 Page 58 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
LYME DISEASE 1 x SST Referred to: PathWest QEII 2mL
LYMPHOCYTE PROLIFERATION ASSAY
1 x Special Collection Kit
Tested at PathWest Immunology. Patient needs to book an appointment with QEII Immunology on 08 6383 4330.
LYMPHOCYTE SUBSETS Yes 1 x ACD and 1 x EDTA
Full tube of ACD (can use 1mL of EDTA ONLY if paediatric or elderly/infirm). See: WI-COLL-96 - Flow Cytometry
6mL ACD
LYMPHOMA STUDIES 1 x Sterile Container
DOCTOR COLLECT Send to Histology department ASAP marked URGENT.
MACRO CK 1 x SST Contact Duty Biochemist 2mL
MACROPROLACTIN 1 x SST See: WI-COLL-63 - Prolactin/Macroprolactin Collection
2mL
MAGNESIUM 1 x SST 2mL
MAGNESIUM (24 HOUR URINE) 1 x Urine Container- 24hr acid
Must be acidified. 2mL
MAGNESIUM (FLUID) 1 x Sterile Container
DOCTOR COLLECT 2mL
MAGNESIUM (URINE SPOT) 1 x Urine Container - random
Does not need to be acidified. Transfer into Vacutainer (no additive) urine tube. Urine tubes must not be filled beyond half way to prevent spillage.
2mL
MALARIAL PARASITES SCREEN (URGENT – Result within 4hours)
1 x EDTA TREAT AS URGENT - Full Name, DOB or URN, DOC & TOC must be on EDTA tube. Collector must sign EDTA tube and request form. TREAT AS URGENT: Needs to reach the lab within 3 hours of collection. NOTE: Only 1 EDTA required for any combination of FBC/Indices, ESR, Malaria, RET, HbA1C, HE.
1ml
MALIGNANCY HCG/QCG 1 x SST 2mL
MANGANESE BLOOD Yes 1 x EDTA dedicated tube
Referred to: Royal Prince Alfred Hospital
2mL
MANGANESE URINE Yes 1 x Urine Container - random
Referred to: Laverty Pathology 5mL
MANNOSE BINDING LECTIN 1 x Plain Tube (Red Top)
Referred to: PathWest QEII 1mL
Specimen Collection Manual Issue 19 Page 59 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
MANTOUX TEST - HUMAN Yes Special Collection Kit
See Mantoux WI-COLL-84 Note: Injection and reading done at the different locations. Injection Only done at Myaree Collection Centre (9 Hayden Court Myaree) on Monday to Wednesday. Reading Patient to attend the main laboratory (74 McCoy Street Myaree) after 48 hours from the injection. No appointments are required.
MARFANS SCREEN 2 x EDTA 6mL - Pink, dedicated tube
Referred to: PathWest QEII Hardcopy result only released to the Doctor.
2mL
MAST CELL TRYPTASE 1 x SST spin separate and freeze ASAP
Referred to: PathWest QEII Transport frozen. Samples should ideally be collected between 15minutes and 3 hours of the suspectd event causing mast cell activation.
1mL
MBA 20 1 x SST SST must be spun within 4 hours of collection. For employment and insurance purposes only.
2mL
MEASLES IMMUNE STATUS 1 x SST If querying measles infection- treat as URGENT and refer to the 'MEASLES INFECTION' entry. If immunity check- treat as routine serology collection.
1 mL
MEASLES INFECTION (CALL BEFORE COLLECTION)
1 x SST Requests for measles infection are considered urgent. Please notify the Duty Microbiologist on 08 9317 0999 whenever a query for measles infection is requested. Not to be collected in ACCs- home collect only.
2 mL
MEASLES PCR (CALL BEFORE COLLECTION)
1 x Dry Swab - Orange/White and 1 x Urine Container - random and 1 x SST
Referred to: PathWest QEII Requests for measles PCR are considered urgent. Please notify the Duty Microbiologist on 08 9317 0999 whenever measles PCR is requested. Not to be collected in ACCs- home collect only.
2mL
Specimen Collection Manual Issue 19 Page 60 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
MECP2 2 x EDTA 6mL - Pink, dedicated tube
Referred to: PathWest FSH Hardcopy report issued to the doctor only.
2mL
MELIODOSIS SEROLOGY 1 x SST Referred to: PathWest QEII 2mL
MENIN 1 x EDTA - dedicated tube required
Referred to: PathWest QEII Patient must be referred by Genetic Services of Western Australia (08) 9340 1525 or Endocrinologist. Sample is only viable for 48Hrs. Only Collect Monday to Thursday. Do not collect on Public Holidays.
2mL
MENINGOCOCCAL PCR 1 x EDTA - dedicated tube required
Referred to: PathWest QEII
2mL
MENOPAUSAL STUDIES 1 x SST 2mL
MEPACRINE STAINING (CALL BEFORE COLLECTION)
1 x Sodium Citrate - DO NOT SPIN, dedicated tube required
Referred to: PathWest FSH Ring Coagulation Dept. prior to collection on 08 9317 0862 or 9317 0861. Place DO NOT SPIN sticker around the lid. Usually requested with Platelet Aggregation Studies. Send to Myaree within 1hour. PathWest FSH do testing Mon to Fri, 8:30 to 5:00. Keep sample at room temp. Transport in Blue bag. Sample stability: 24hrs at Room temp. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
MERCURY BLOOD 1 x Trace Element Free - DO NOT SPIN
Referred to: Laverty Pathology Place DO NOT SPIN sticker around the lid.
2mL
MERCURY URINE 1 x Urine Container
Referred to: Laverty Pathology 10mL
MESOTHELIN Yes 1 x SST spin separate and freeze
Referred to: QML Pathology Provide clinical details such as exposure to asbestos.
2mL
METABOLIC BONE STUDIES (FMBS)
2 x SST - DO NOT OPEN and 2 x PPT and 1 x Urine Container - random
Bloods and urine need to be collected within 2 hours of each other. Must be a pristine SST tube for ionised calcium – place DO NOT OPEN sticker around the lid. See: WI-COLL-92 - Bone Metabolic Studies Collection
2mL each
METABOLIC URINE SCREEN 1 x Urine Container - dedicated jar required. Freeze ASAP
Referred to: PathWest QEII
5mL
Specimen Collection Manual Issue 19 Page 61 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
METANEPHRINES BLOOD See CATECHOLAMINES BLOOD
METANEPHRINES URINE See CATECHOLAMINES URINE
METHADONE 1 x Plain Tube (Red Top)
Referred to: PathWest QEII Take sample prior to next dose.
2mL
METHAEMOGLOBIN 1 x DOCTOR COLLECT
Test is performed at the hospital sites and must be analysed immediately. Please refer the patient to the nearest hospital.
0.5mL
METHOTREXATE (URGENT – Result within 4hours)
1 x Lithium Heparin
Referred to: PathWest 1mL
METHYLMALONIC ACID URINE 1 x Urine Container - dedicated jar required. Freeze ASAP
Referred to: PathWest QEII
5mL
METHYMALONIC ACID Test Not Available 5ml
MICROALBUMIN 1 x Urine Container - random
Transfer into Vacutainer (no additive) urine tube. Urine tubes must not be filled beyond half way to prevent spillage.
2mL
MICROALBUMIN (24 HOUR URINE)
1 x Urine Container- 24hr plain or 1 x Urine Container- 8 hr plain
Note on the form, the start time and the end time of collection period.
2mL
MICROARRAY - AMNIOTIC FLUID, CVS
DOCTOR COLLECT Referred to QML Pathology Contact Genetics Dept. for queries on 08 9317 0922. Specialised container: Amniotic fluid> 10mL sterile black top tube. POC & CVS> 70mL red top sterile jar (NO formalin) containing PBS and heparin.
MICROARRAY - BLOOD 1 x EDTA - dedicated tube required
Referred to: QML Pathology Use paediatric tube for small or difficult collects.
3mL
MICROARRAY - PRODUCT OF CONCEPTION (POC)
Sterile Container- Genetics
Referred to: QML Pathology Contact Genetics Dept. for queries on 08 9317 0922. Specialised container: 70mL red top sterile jar (NO formalin) containing PBS and heparin.
Specimen Collection Manual Issue 19 Page 62 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
MICROSOMAL ANTIBODIES 1 x SST 2mL
MITOCHONDRIAL ANTIBODY 1 x SST 1mL
MITOCHONDRIAL DISORDER 1 x EDTA 6mL - Pink, dedicated tube
Referred to: PathWest QEII 2mL
MIXED LYMPHOCYTE CULTURE No performed at WDP. Patient must book test with PathWest Immunology on 08 6383 4330
MLH1 GENE ANALYSIS 1 x EDTA 6mL - Pink, dedicated tube
Referred to: PathWest FSH Patient must be referred by Genetic Services of WA (08) 6458 1525.
MODY GENE TESTING Yes 2 x EDTA - dedicated tube required
Referred to: Mater Pathology (QLD) 4mL
MOLLUSCUM CONTAGIOSUM PCR 1 x Dry Swab - Orange/White
Referred to: PathWest QEII
MOLYBDENUM - BLOOD 1 x Trace Element Free - DO NOT SPIN
Referred to: Royal North Shore Hospital Place DO NOT SPIN sticker around the lid. Keep sample cold, transport on ice brick.
2mL
MORPHINE - SERUM 1 x Lithium Heparin spin, separate & freeze ASAP
Referred to: Royal Prince Alfred Hospital
1mL
MPL MUTATION 2 x EDTA 6mL - Pink, dedicated tube
Referred to: Genomic Diagnostics When asked with JAK 2 Mutation and/or MPL Mutation only 2 EDTA 6ml Pink Top tubes required altogether.
6mL
Specimen Collection Manual Issue 19 Page 63 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
MRSA CLEARANCE Swab - Blue Top Gel
All swabs to be placed into gel for transport back to laboratory after the following procedure and DO NOT REFRIGERATE Swab from Nostrils (see NASAL SWAB MC&S for detailed instruction) • Moisten the swab using ONLY
with either sterile water or sterile saline (DO NOT USE TAP WATER)
• Use one swab moistened in sterile water or sterile saline for both nostrils
Swab for Throat (see WI-COLL-72) • Use one swab
Dry Lesions (if present) • Moisten the swab using ONLY
with either sterile water or sterile saline (DO NOT USE TAP WATER)
Discharging/ Moist Lesions (if present) • Use one swab
Where MRSA clearance requested • Collect two sets of nasal and
throat swabs and other applicable sites (wound etc.).
• The two sets of swabs should be collected consecutively.
• Each consecutive set must be given a separate request number and labelled set 1 & Set 2.
MRSA MC&S Swab - Blue Top Gel
See MRSA WI-COLL-85 DO NOT REFRIGERATE
Specimen Collection Manual Issue 19 Page 64 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
MRSA SCREEN Swab - Blue Top Gel
All swabs to be placed into gel for transport back to laboratory after the following procedure and DO NOT REFRIGERATE Swab from Nostrils (see NASAL SWAB MC&S for detailed instruction) • Moisten the swab using ONLY
with either sterile water or sterile saline (DO NOT USE TAP WATER)
• Use one swab moistened in sterile water or sterile saline for both nostrils
Swab for Throat (see WI-COLL-72) • Use one swab
Dry Lesions (if present) • Moisten the swab using ONLY
with either sterile water or sterile saline (DO NOT USE TAP WATER)
Discharging/ Moist Lesions (if present) • Use one swab
MTHFR GENE TEST Yes 2 x EDTA 6mL - Pink, dedicated tube
Referred to: Genomic Diagnostics If there is no first degree relative or a personal history of thrombosis (DVT) stated on request form there will be an out of pocket fee.
6mL
MUCOPOLYSACCHARIDE 1 x Urine Container - dedicated jar required. Freeze ASAP
Referred to: PathWest QEII Freeze ASAP and transport Frozen
5mL
MUMPS PCR 1 x Dry Swab - Orange/White and 1 x Urine Container
Referred to: PathWest QEII
MUMPS SEROLOGY 1 x SST 1 mL
MURRAY VALLEY ENCEPHALITIS 1 x SST Referred to: PathWest QEII 2mL
MUSCLE ENZYME 1 x SST 2mL
MUSK ANTIBODIES 1 x SST Referred to: PathWest QEII 1mL
Specimen Collection Manual Issue 19 Page 65 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
MYCOBACTERIAL CULTURE Urine Container - random or Urine Container
See Mycobacterium WI-COLL-86
MYCOPHENOLIC ACID 1 x Lithium Heparin
Referred to: PathWest QEII Take sample prior to dose.
2mL
MYCOPLASMA GENITALIUM PCR 1 x Urine Container or 1 x Dry Swab - Orange/White
See WI-COLL-115 & WI-COLL-81 Swabs collected from genital sites are collected by the referring doctor.
MYCOPLASMA PNEUMONIAE PCR 1 x Dry Swab - Orange/White or 1 x Swab - Viral Transport Media
See WI-COLL-115 Note: This test is only performed on specific request for respiratory Mycoplasma (Mycoplasma pneumoniae)
MYCOPLASMA SEROLOGY 1 x SST 1 mL
MYCOPLASMA/UREAPLASMA CULTURE
Urine Container or DOCTOR COLLECT
Urine Samples WI-COLL-82 Swab collections are performed by doctor collects
MYELOMA SCREEN 1 x SST and 1 x EDTA
Do not use torniquet for calcium collection.
5mL
MYOGLOBIN 1 x Plain Tube (Red Top)
Referred to: QML Pathology 5mL
MYOSITIS SPECIFIC A/B 1 x SST Referred to: PathWest QEII 1mL
NALTREXONE Yes 1 x Plain Tube (Red Top) - Spin, Separate & Freeze
Referred to: Forensic Science at ChemCentre Advise patient to contact the Forensic Science at ChemCentre on 08 9422 9800 for pricing enquires.
1mL
NASAL SWAB MC&S Swab - Blue Top Gel
1. Advise patient to sit and have their head rested against the wall
2. Provide tissue to the patient 3. Use one moistened swab using
ONLY with either sterile water or sterile saline (DO NOT USE TAP WATER) for both nostrils
4. Insert the swab 1 - 2cm until all the cotton wool is in the nose
5. Press the swab to the centre so it is against the septum.
6. Rotate 360 ° clockwise then anticlockwise.
7. Remove and then sample through the other nostril cavity in the same way.
8. Place swab in transport gel and DO NOT REFRIGERATE
Specimen Collection Manual Issue 19 Page 66 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
NATURAL KILLER CELLS 1 x ACD and 1 x EDTA
Full tube of ACD (can use 1mL of EDTA ONLY if paediatric or elderly/infirm). See: WI-COLL-96 - Flow Cytometry
6mL ACD
NEONATAL BLOOD GROUP EDTA 6mL - Pink, dedicated tube
1. Full Name, DOB or URN, DOC & TOC must be on EDTA tube and request form. All information must match between form and sample.
2. Collector must sign the tube and certifier on form - these signatures must match.
Contact Transfusion Medicine Dept. on 08 9321 3300 for any enquiries.
1 mL
NEUROACANTHOCYTOSIS (URGENT – Result within 4hours)
1 x EDTA The EDTA must at the lab within 3 hours and the result reported within 4 hours. If in doubt, please ring Haematology Dept. on 08 9317 0861
4 mL
NEURON SPECIFIC ENOLASE 1 x SST spin separate and freeze
Referred to: PathWest FSH 0.5mL
NEUTROPHIL ANTIBODIES 1 x EDTA - dedicated tube required and 1 x Plain Tube (Red Top)
Referred to: PathWest FSH Sample must be at PathWest within 24hours of collection. Only collect Monday to Thursday. Do not collect on Public Holidays.
1mL each
NEUTROPHIL FUNCTION Not done at WDP. Patient is to book with PathWest Immunology on 08 6383 4330
NICKEL BLOOD 1 x Trace Element Free - DO NOT SPIN
Referred to: Royal North Shore Hospital Place DO NOT SPIN sticker around the lid.
0.5mL
NICKEL URINE Yes 1 x Urine Container - random
Referred to: Laverty Pathology 10mL
NICOTINE Yes 1 x Urine Container - dedicated jar required. Freeze ASAP
Referred to: Laverty Pathology 5mL
NIPPLE DISCHARGE DR COLLECT Gently squeeze the nipple until fluid appears. Smear fluid directly onto glass slide. Allow slides to air dry. Ensure slide(s) are labelled with patient name, DOB and site (Left or Right nipple).
Specimen Collection Manual Issue 19 Page 67 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
NITRAZEPAM 1 x Plain Tube (Red Top)
Referred to: QML Pathology Collect just prior to the next dose or at least 6 hours after the last dose. Provide clinical and medication detail, including time and date of last dose.
2mL
NK CELLS 1 x ACD and 1 x EDTA
Full tube of ACD (can use 1mL of EDTA ONLY if paediatric or elderly/infirm). See: WI-COLL-96 - Flow Cytometry
6mL ACD
NOROVIRUS GI/GII PCR 1 x Faeces Container
See WI-COLL-115
NORTRITYLINE 1 x Plain Tube (Red Top)
Referred to: QML Pathology Collect immediately prior to next dose. Provide clinical and medication details including time and date of next dose.
2mL
NSD1 GENE EDTA Referred to: PathWest QEII
NT-PROBNP (PLEURAL FLUID) DOCTOR COLLECT 2 mL
NTX 1 x Urine Container - random and 1 x PPT - spin and freeze
4 mL
NUCLEOSOME ANTIBODIES 1 x SST Referred to: Laverty Pathology Only if ANA positive.
1mL
OLANZAPINE 1 x Plain Tube (Red Top)
Referred to: PathWest QEII Take prior to next dose.
1mL
OLIGOSACHARIDES URINE 1 x Urine Container - dedicated jar required. Freeze ASAP
Referred to: Adelaide Women's and Children's Hospital
10mL
OMMA 1 x SST 2mL
OSPOLOT 1 x Plain Tube (Red Top)
Referred to: QML Pathology Collect sample a minimum of 8 hours after the last dose or immediately prior to the next dose.
1mL
OSTEOCALCIN Yes 1 x SST spin separate and freeze
4mL
NT-PROBNP Yes 1 x SST NOTE: This is not NT-PROBNP 2 mL
OVARIAN ANTIBODIES 1 x SST Referred to: PathWest QEII 1ml
OVARIAN CYST ASPIRATE CYTOLOGY
DR COLLECT
OVARIAN TUMOUR MARKER 1 x SST 2mL
Specimen Collection Manual Issue 19 Page 68 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
OVULATION TRACKING (URGENT – Result within 4hours)
Yes 1 x SST Must be bled before 9am to ensure same day results. See: WI-COLL-62 “Ovulation Tracking - Biochemistry”
2mL
OXALATE 1 x Urine Container- 24hr acid
Referred to: PathWest FSH Give patient 2x 24hr urine bottle containers with acid.
10mL
OXAZEPAM 1 x Plain Tube (Red Top)
Referred to: QML Pathology Collect just prior to the next dose or at least 6 hours after the last dose. Provide clinical and medication detail, including time and date of last dose.
2mL
OXYHAEMOGLOBIN DOCTOR COLLECT Test is performed at the hospital sites and must be analysed immediately. Please refer the patient to the nearest hospital.
0.5mL
OXYPURINOL 1 x EDTA - dedicated tube required
Referred to: St Vincent's Pathology 2mL
P1NP 1 x SST 4mL
PANCREATIC ANTIBODIES 1 x SST Referred to: PathWest QEII 2mL
PANCREATIC ENZYMES 1 x SST 2mL
PANCREATIC POLYPEPTIDE 1 x SST spin separate and freeze
Referred to: Royal Prince Alfred Hospital
1mL
PANCREATIC TUMOUR MARKER 1 x SST 2mL
PAP SMEAR CYTOLOGY DOCTOR COLLECT Pap smear cytology is no longer available and is not covered by Medicare. See CERVICAL SCREENING TEST for details on the new screening program requirements.
PARACETAMOL 1 x SST Always treated as URGENT. Testing performed at JND & PHC. JOONDALUP (08 9400 9810) or PEEL HEALTH CAMPUS (08 9531 8510)
0.5mL
PARAINFLUENZA SEROLOGY 1 x SST Referred to: QML Pathology 1mL
PARAPROTEIN 1 x SST 2mL
PARAQUAT URINE 1 x Urine Container - random
Referred to: PathWest QEII 4mL
PARASITE ID Urine Container - random
PARATYPHOID 1 x Faeces Container
Referred to: PathWest QEII 1mL
Specimen Collection Manual Issue 19 Page 69 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
PARECHOVIRUS PCR 1 x Dry Swab - Orange/White or Faeces Container
Referred to: PathWest QEII 1mL
PARIETAL CELL ANTIBODY 1 x SST 1mL
PARVOVIRUS B19 SEROLOGY 1 x SST 2mL
PARVOVIRUS PCR 1 x EDTA - dedicated tube required
Referred to: PathWest FSH 2mL
PCB LEVELS Yes 1 x Urine Container - random and 1 x Lithium Heparin - DO NOT SPIN
Referred to: ChemCentre Place DO NOT SPIN sticker around the lid.
PDGFR ALPHA 1 x Lithium Heparin and 2 x EDTA 6mL - Pink, dedicated tube
Referred to: PathWest FSH 2mL each
PERHEXILINE 1 x Plain Tube (Red Top)
Referred to: PathWest QEII Provide clinical and medication details. Collect just prior to next dose.
0.5mL
PERIPHERAL NATURAL KILLER CELLS
1 x ACD and 1 x EDTA
Full tube of ACD (can use 1mL of EDTA ONLY if paediatric or elderly/infirm). See: WI-COLL-96 - Flow Cytometry
6mL ACD
PERITONEAL FLUID/WASHINGS DR COLLECT
PERNICIOUS ANAEMIA 1 x SST 1mL
PESTICIDES Yes 1 x EDTA - dedicated tube required
Referred to: WorkCover NSW 5mL
PET SCREEN 1 x EDTA and SST and Urine Container - random
2mL
PETHIDINE URINARY 1 x Urine Container - random
Referred to: PathWest QEII 2mL
PF 1+2,TAT, CAT 3 x Sodium Citrate Referred to: PathWest FSH 3mL
PF1, 2 See PROTHROMBIN FRAGMENT 1 + 2 entry for collection requirements. See: FRM-COLL-86 - Coagulation Collection Guide
Specimen Collection Manual Issue 19 Page 70 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
PFA (100 OR 200) (URGENT – Result within 4hours)
See PLATELET FUNCTION ANALYSIS entry for collection requirements. See: FRM-COLL-86 - Coagulation Collection Guide
PGX MH/PAIN/MULTI Yes 1 x EDTA - dedicated tube required
Referred to Genomics Diagnostics Payment to be made prior to collection via online or phone – 1800 822 999 (9-5pm AEST).
4mL
PH (FLUID) 1 x Sterile Container
DOCTOR COLLECT 2mL
PHENOBARBITONE 1 x Plain Tube (Red Top)
Referred to: PathWest QEII 0.5mL
PHENYLALANINE 1 x Lithium Heparin - DO NOT SPIN
Referred to: PathWest QEII Place DO NOT SPIN sticker around the lid.
1mL
PHENYLKETONURIA URINE 1 x Urine Container - random
Referred to: PathWest QEII 10mL
PHENYTOIN 1 x SST Record date and time of last dose of medication on the form.
2mL
PHOSPHATE 1 x SST 2mL
PHOSPHATE (24 HOUR URINE) 1 x Urine Container- 24hr acid
Must be acidified urine. 2mL
PHOSPHATE (FLUID) DOCTOR COLLECT 2mL
PHOSPHATE (URINE SPOT) 1 x Urine Container - random
Transfer into Vacutainer (no additive) urine tube. Urine tubes must not be filled beyond half way to prevent spillage.
2mL
PHOSPHOLIPASE 2 RECEPTOR ANTIBODY/AUTOANTIBODY
1 x Plain Tube (Red Top)
Referred to: PathWest QEII 2mL
PICA 1 x SST Referred to: QML Pathology 2mL
PK ASSAY 1 x EDTA - dedicated tube required
Referred to: CHW 2mL
PLA 2 ANTIBODIES
PLACENTA-LIKE GROWTH FACTOR (PLGF)
Yes 1 x SST - Spin and freeze ASAP
1. Acceptable gestational age: 8 weeks 0 days to 14 weeks 6 days.
2. Fill out Antenatal Patient Questionnaire (FRM-COLL-6).
3. If First Trimester Screen (FTS) or Triple Test/ Neural Tube Defect is also co-requested, 1x SST is sufficient.
4 mL
Specimen Collection Manual Issue 19 Page 71 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
PLASMA 5HIAA Serum/plasma 5HIAA not available. Collect and see SEROTONIN – PLATELET instead.
PLASMA METANEPHRINES See CATECHOLAMINES
PLASMA ORGANIC ACID SCREEN 2mL
PLASMA PHOSPHOLIPIDS 1 x Sodium Citrate NOTE: This is not ANTIPHOSPHOLIPID ANTIBODIES. Transport in a blue bag to arrive at the lab within 4 hours. Otherwise, single spin, separate and freeze as per WI-COLL-89
2.7mL
PLASMINOGEN ACTIVATOR INHIBITOR-1
2 x Sodium Citrate This is a referred test. Must be at Myaree within 4hrs - transport in blue bag. Or single spin/separate/freeze and transport frozen. See: FRM-COLL- 86 - Coagulation Collection Guide
2.7mL
PLASMIROFEN ACTIVATOR INHIBITOR
2 x Sodium Citrate - Spin, Separate & Freeze ASAP
Referred to: Royal Prince Alfred Hospital
PLATELET AGGREGATION STUDIES (CALL BEFORE COLLECTION)
4 x Sodium Citrate - DO NOT SPIN
Ring Coagulation Dept. prior to collection on 08 9317 0862 or 9317 0861. Must be at Myaree lab within 1hr. Transport samples at room temp. Place DO NOT SPIN sticker around the lids. See: FRM- COLL-86 - Coagulation Collection Guide
2.7mL each
PLATELET COUNT 1 x EDTA Full Name, DOB or URN, DOC & TOC must be on EDTA tube. Collector must sign EDTA tube and request form. If "citrated platelet count" is requested, then collect 1x sodium citrate with 1x EDTA. NOTE: Only 1 EDTA required for any combination of FBC/Indices, ESR, Malaria, RET, HbA1C, HE
2.7mL
PLATELET DENSE GRANULES See MEPACRINE STAINING entry for collection requirements. See: FRM-COLL-86 - Coagulation Collection Guide
PLATELET FUNCTION ANALYSIS (URGENT – Result within 4hours)
2 x Sodium Citrate - DO NOT SPIN
Place DO NOT SPIN sticker around the lids. Transport in blue bag. Must be at Myaree lab within 4hrs. Patient to complete FRM-COLL-43 See: FRM- COLL-86 - Coagulation Collection Guide
2.7mL each
Specimen Collection Manual Issue 19 Page 72 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
PLATELET MICROPARTICLES 2 x Sodium Citrate Referred to: PathWest at FSH. Samples to lab within 4hrs. Transport in blue bag. Or single spin/separate/freeze. Transport on dry ice. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL each
PLEURAL FLUID DOCTOR COLLECT
PML-RARA PCR 2 x EDTA 6mL - Pink, dedicated tube
Referred to: PathWest FSH Collect only Monday to Thursday. Do not collect on public holidays. Store at room temperature.
3mL
PNEUMOCOCCAL AB 1 x SST Referred to: PathWest QEII 2mL
PNEUMOCYSTIS JIROVECI (CARINII) PCR
PNA tube/sterile container
See WI-COLL-115 This test is only performed on specific request for respiratory PCP
PNH SCREEN 2 x EDTA - dedicated tube required
Referred to: PathWest QEII Only collect Monday - Thursday. Do not collect on public holidays.
0.5mL
POLYOMA VIRUS 1 x Urine Container - random
Referred to: PathWest QEII
POMPES DISEASE Yes 1 x Urine Container - random and 1 x EDTA - dedicated tube required
Referred to: Adelaide Women's and Children's Hospital
2mL each
PORPHOBILINOGEN SCREEN 1 x Urine Container - random
Referred to: PathWest QEII Wrap in foil to protect from light. Keep cold and transport on ice brick.
1mL
PORPHYRINS 2 x EDTA and 1 x Urine Container - random
Referred to: PathWest QEII Wrap EDTA and urine samples in foil to protect from light. Keep cold and transport on ice brick.
2mL blood 10mL urine
PORPHYRINS FAECES 1 x Faeces Container
Referred to: Royal Prince Alfred Hospital Wrap in foil and freeze ASAP. Transport frozen.
Specimen Collection Manual Issue 19 Page 73 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
POTASSIUM 1 x SST Spin within 4 hours of collection. Do not transport on an ice brick.
2mL
POTASSIUM (FLUID) DOCTOR COLLECT 2mL
POTASSIUM (PLASMA) 1 x Lithium Heparin
2mL
POTASSIUM (URINE 24 HOUR) 1 x Urine Container- 24hr plain
Does not need to be acidified. 2mL
POTASSIUM (URINE SPOT) 1 x Urine Container - random
Transfer into Vacutainer (no additive) urine tube. Urine tubes must not be filled beyond half way to prevent spillage.
2mL
POUCH OF DOUGLAS FLUID DOCTOR COLLECT
PRADER WILLI ANALYSIS Yes* 1 x EDTA - dedicated tube required
For WA patients – no out-of-pocket fees and referred to PathWest QEII. *For NT patients – there will be an out-of-pocket fee and referred to Women and Children’s Hospital Adelaide.
2mL
PRE-ECLAMPSIA SCREEN 1 x EDTA and SST and Urine Container - random
2mL
PRO INSULIN 1 x SST spin separate and freeze
Referred to: SEALS (PRINCE OF WALES, NSW)
2mL
PROCALCITONIN Yes Metro (within 48H) 1 x SST or Regional 1 x SST spin separate and freeze
Spin, separate and freeze if > 48 hours 0.5mL
PROCOLLAGEN TYPE 3 NT Yes 1 x SST Referred to: Sullivan Nicolaides (QLD) 2mL
PROGESTERONE 1 x SST 2mL
PROLACTIN 1 x SST Patient MUST rest for 20 minutes prior to test. See: WI-COLL-63 Prolactin/Macroprolactin Collection
2mL
Specimen Collection Manual Issue 19 Page 74 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
PROSTATE HEALTH INDEX (PHI) Yes 1 x SST Referred to: QML Pathology 4mL
PROTEIN 1 x SST 2mL
PROTEIN (CSF) (URGENT)
DOCTOR COLLECT 2mL
PROTEIN (FLUID) DOCTOR COLLECT 2mL
PROTEIN C Yes 2 x Sodium Citrate To Myaree within 10hrs. Transport in blue bag. Or single spin/separate/freeze. Patient to complete Questionnaire: FRM-COLL- 43. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
PROTEIN CREATININE RATIO 1 x Urine Container - random
Transfer into Vacutainer (no additive) urine tube. Urine tubes must not be filled beyond half way to prevent spillage.
2mL
PROTEIN CREATININE RATIO (24 HOUR URINE)
1 x Urine Container- 24hr plain
2mL
PROTEIN S Yes 2 x Sodium Citrate To Myaree within 10hrs. Transport in blue bag. Or single spin/separate/freeze. Patient to complete Questionnaire: FRM-COLL- 43. If unsure of collection requirements, phone Coagulation Dept. on 08 9317 0862. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
PROTHROMBIN FRAGMENT 1 + 2 2 x Sodium Citrate Referred to: PathWest Must be to a lab within 4 hrs. Transport in blue bag. Or DOUBLE spin/separate/freeze. Patient to complete questionnaire: FRM-COLL- 43. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
PROTHROMBIN G20210A MUTATION
Yes 1 x EDTA 6mL - Pink, dedicated tube
Referred to: Genomic Diagnostics This test is performed in same assay with Factor V Leiden mutation. The same charge applies whether testing for just Prothrombin G20210A mutation or together with Factor V Leiden. Eligible for Medicare rebate if patient has had a proven venous thrombosis or pulmonary embolism or has a first degree relative with the mutation.
6mL
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PROTHROMBIN TIME (PT), INTERNATIONAL NORMALISED RATIO (INR)
1 x Sodium Citrate For patients NOT on Warfarin (Coumadin or Marevan). To lab within 24 hours - transport in blue bag. Or single spin/separate/freeze. Patient to complete questionnaire: FRM-COLL-43. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
PSA Yes 1 x SST Medicare will pay a rebate for only one (1) PSA test performed in any 12- month period in the absence of previously abnormal results or previously diagnosed prostatic disease. A non-refundable fee will be charged if not billed through Medicare. Request form to be stamped with "Patient Advised of Fee" and signed by the patient acknowledging that an out of pocket payment may be due. See: Patient to complete FRM-COLL-36 - Prostate Specific Antigen (PSA) Patient Questionnaire
2mL
PTH 1 x PPT spin within 30 minutes of collection and 1 x SST
Spin within 30 minutes of collection (DO NOT FREEZE OR SEPARATE) Collect 1 x SST for concurrent calcium testing (even if not requested)
2mL
PTH RELATED PEPTIDE (PTHRP) Test Not Available
PYRROLE URINE TEST Yes DO NOT ACCEPT SAMPLE
Advise patient to contact SAFE Analytical Laboratories on 07 5522 1919 for collection kit. DO NOT accept the sample from patient.
PYRUVATE KINASE 1 x EDTA 6mL - Pink, dedicated tube
The referral laboratory will only offer the test after consultation with the Haematologist at the Children’s Hospital at Westmead (NSW). The requesting doctor must call the Haematologist on (02) 9845 0000 prior to requesting the test.
2mL
Q FEVER 1 x SST Only for Immunity/ pre or post vaccination done in-house. Q Fever requests which are acute or diagnostic e.g. query Q Fever or IgM requested are referred to PathWest QEII.
2mL
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Vol
QEP 1 x SST 2mL
QF-PCR - AMNIOCENTESIS, CVS, OTHER
Yes DOCTOR COLLECT QF-PCR requests to send to QML: will be FISH testing Out of pocket - if not covered by medicare Specialised container: Sterile 10mL black top tube: contact Genetic Dept. 08 9317 0922
QF-PCR - BLOOD 1 x EDTA 6mL - Pink, dedicated tube
Please confirm with QML/WDP genetics this may need to go to PathWest QEII dept diagnostic genomics.
6mL
QUANTIFERON TB GOLD ASSAY Yes 1 x Special Collection Kit
See WI-COLL-49 - Quantiferon Gold Test for TB Immunity for collection method. Patient to complete FRM-COLL-33 Quantiferon Gold Patient Questionnaire
1mL
QUETIAPINE 1 x Lithium Heparin - DO NOT SPIN
Referred to: PathWest QEII Place DO NOT SPIN sticker around the lid.
0.5mL
RABIES 1 x SST Referred to: ICPMR Westmead (NSW) 5mL
RAST Yes 1 x SST Most requests will be fully Medicare rebateable but there will be out-of-pocket for non-specialist requests for more than 4 tests. See FRM-COLL-104 for out-of-pocket fees and test names. Contact Immunology Dept. on 08 9317 0920 for any pricing queries.
2mL
RBC COPPER 1 x Trace Element Free - DO NOT SPIN
Referred to: Royal North Shore Hospital (NSW) Place DO NOT SPIN sticker around the lid.
5mL
RBC MAGNESIUM 1 x Trace Element Free - DO NOT SPIN
Referred to: Royal Prince Alfred Hospital Place DO NOT SPIN sticker around the lid.
5mL
RBC SELENIUM 1 x Trace Element Free - DO NOT SPIN
Referred to: Royal North Shore Hospital (NSW) Place DO NOT SPIN sticker around the lid.
2mL
RBC ZINC 1 x Trace Element Free - DO NOT SPIN
Referred to: Royal Prince Alfred Hospital Place DO NOT SPIN sticker around the lid.
5mL
RECTAL SWAB MC&S DOCTOR COLLECT DO NOT REFRIGERATE
Specimen Collection Manual Issue 19 Page 77 of 95
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Vol
RED CELL PHENOTYPE EDTA 6mL - Pink, dedicated tube
1. Full Name, DOB or URN, DOC &TOC must be on EDTA tube andrequest form. All information mustmatch between form and sample.
2. Collector must sign the tube andcertifier on form - these signaturesmust match.
Contact Transfusion Medicine Dept. on 08 9321 3300 for any enquiries.
6 mL
REDUCING SUBSTANCES 1 x Faeces Container - freeze within 4 hours from collection
Only available for children under 11 years of age. 11+ Test not available (TNA ). Specimen should be frozen within 4 hours from collection. Require small quantity of fresh faeces (e.g. at least pea size) Advise patient’s guardian NOT to obtain directly from absorbent napkin liner as detergents cause false results. Collect from impervious material such as polythene and include fluid at present.
1g
RENIN 1 x PPT - spin and freeze ASAP
See WI- COLL 105 2mL
REPTILASE TIME 1 x Sodium Citrate To lab within 24 hrs. Transport in blue bag. Or single spin/separate/freeze and transport frozen. See: FRM-COLL- 86 - Coagulation Collection Guide
2.7mL
RESPIRATORY SYNCITIAL VIRUS 1 x SST Referred to: PathWest QEII 3mL
RESPIRATORY VIRUS PCR 1 x Dry Swab - Orange/White or 1 x Swab - Viral Transport Media
See WI-COLL-115 & FRM-COLL-81 (Swab Collection guide)
RETICULIN ANTIBODY 1 x SST RETICULIN ANTIBODY testing is no longer performed. Please refer to COELIAC SEROLOGY.
RETICULOCYTE COUNT 1 x EDTA Full Name, DOB or URN, DOC & TOC must be on EDTA tube. Collector must sign EDTA tube and request form. NOTE: Only 1 EDTA required for any combination of FBC/Indices, ESR, Malaria, RET, HbA1C, HE.
1ml
REVERSE T3 Yes 1 x SST spin separate and freeze
Referred to: QML Pathology 2mL
RHEUMATOID FACTOR 1 x SST 2mL
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Vol
RICKETTSIA 1 x SST Referred to: PathWest QEII 2mL
RIDAUIA URINE Test Not Available
RISPERIDONE 1 x Lithium Heparin - DO NOT SPIN
Referred to: PathWest QEII Place DO NOT SPIN sticker around the lid.
1mL
RISTOCETIN COFACTOR See FACTOR STUDIES entry for Collection requirements. See: FRM- COLL-86 - Coagulation Collection Guide
RNA POLYMERASE 1 x SST Referred to: IMVS 1mL
ROSS RIVER PCR 1 x EDTA - dedicated tube required
Referred to: PathWest QEII 1mL
ROSS RIVER VIRUS (RRV) 1 x SST 1 mL
ROTAVIRUS PCR 1 x Faeces Container
See WI-COLL-13
RUBELLA 1 x SST 2 mL
RUBELLA PCR (CALL BEFORE COLLECTION)
1 x EDTA or 1 x Dry Swab - Orange/White
Referred to: PathWest QEII Requests for Rubella PCR are considered urgent. Please notify the Duty Microbiologist on 08 9317 0999 whenever Rubella PCR is requested.
1mL
RUSSELL SILVER SYNDROME 1 x EDTA - dedicated tube required
Contact Genetics Dept. on 08 9317 0922 for any enquires.
2mL
SABRIL 1 x Plain Tube (Red Top) - Spin, Separate & Freeze
Referred to: RPA 2mL
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Vol
SALBUTAMOL Yes 1 x Urine Container
Referred to: QML Pathology Urine specimen should be collected as per protocol on Chain-of-Custody form into a tamper-proof container or container enclosed in a tamper- evident bag. Ensure chain-of- custody form is completed. The original copy must always accompany the primary sample to the testing laboratory. NB: Due to possible legal implications, contact the QML Laboratory (07 3121 4419) if the correct procedure cannot be followed. As there is no Medicare rebate for this test, the patient will be invoiced for the cost of the test. If the patient does not accept the cost, do not collect a specimen for this test and advise the patient to discuss this with their doctor. This test is referred to and performed by QML (Queensland Medical Laboratory) See: WI-COLL-53 - Drugs of Abuse Collection Manual
20mL
SALICYLATE 1 x SST Always treated as URGENT. Testing performed at JND. NOTE ON REQUEST FORM: (1)time, date and amount of last dose (2) route - IV or IM (3) age, height, weight and gender of patient ***** FOR REGIONAL COLLECTION CENTRES ***** Please refer the patient to the nearest hospital. JOONDALUP (08 9400 9810)
0.5mL
SALIVARY AND PANCREATIC ISOENZYMES
1 x Lithium Heparin spin, separate & freeze ASAP
Referred to: The Children’s Hospital Westmead
2mL
SALIVARY CORTISOL Yes 1 x Special Collection Kit
Provide patient with FRM-COLL-16 and the salivette tube. Order salivette tube from Manual Biochemistry on 08 9317 0838
1mL
SALIVARY HORMONES 1 x Urine Container
OOP = $25 per hormone for the first 5 hormones, $15 additional hormones, $30 handling fee. This test is referred to and performed by Healthscope Pathology (Incorp ARL)
0.5mL
Specimen Collection Manual Issue 19 Page 80 of 95
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Vol
SALMONELLA MC&S Faeces Container or Blood Culture Bottles
Faeces: Keep refrigerated and see WI-COLL-78 for collection method Blood Cultures: Keep at room temperature and DO NOT REFRIGERATE and see WI-COLL-74
SALMONELLA SEROLOGY 1 x SST Referred to: Prince of Wales Hospital (NSW)
2mL
SAP 1 x SST 2mL
SBR 1 x SST 2mL
SCHISTOSOMA (URINE) Urine Container Coolection instructions for Schistosoma Ova in Urine: The maximum ova secretion is between 1200 hrs and 1500 hrs. Procedure • Ask the patient to collect a 10 - 30mL terminal (end) stream urine using a 70ml yellow cap urine jar between 1200hrs and 1500hrs • Process request as usual for a urine sample See: WI-COLL-75 - Schistosoma Ova in Urine
10mL
SCHISTOSOMA AB (SEROLOGY) 1 x SST Referred to: QML Pathology or PathWest QEII (if specifically requested by doctor)
1mL
SCN1A
SCRAPINGS - CYTOLOGY DOCTOR COLLECT
SELENIUM 1 x Trace Element Free - spin & separate
Referred to: Laverty Pathology 6mL
SELENIUM URINE 1 x Urine Container - random
Referred to: Laverty Pathology 20mL
SEMEN ANALYSIS (POST VASECTOMY)
Seminal Container DO NOT REFRIGERATE. Provide patient with Semen analysis WI-COLL-69 See FRM-COLL-4 for collection method.
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Vol
SEMEN ANLYSIS (FERTILITY STUDIES) (URGENT – Result within 2hours)
Seminal Container DO NOT REFRIGERATE. Delivery must be within 1 hour of collection to specified laboratories.
Provide patient with Semen analysis WI-COLL-69 See FRM-COLL-4 for collection method.
Note: If HALO (DNA defragmentation) is requested along with semen analysis then patient should be directed to Concept Fertility to have the semen analysis done with the HALO test.
Note: MAR refers to SEMEN ANTIBODY testing.
SEMINAL FRUCTOSE 1 x Urine Container
Referred to: PathWest QEII
SERENACE LEVEL
SEROTONIN - PLATELET 2 x EDTA - dedicated tube required
Referred to: PathWest FSH Collect only Monday – Thursday. DO NOT collect on public holidays. Must be at PathWest FSH within 24 hours.
4mL
SEROTONIN SERUM
SERTRALINE Test Not Available
SERUM IMMUNOFIXATION 1 x SST 4mL
SERUM OSMOLALITY 1 x SST Serum and urine osmolality must be collected on the same day if both tests are requested. Joondalup, Hollywood and Peel Health Campus also perform Osmolality testing.
2mL
SERUM PROTEIN ELECTROPHORESIS
1 x SST 4mL
SERUM TRANSFERRIN Yes 1 x Lithium Heparin - DO NOT SPIN
Referred to: PathWest FSH Place DO NOT SPIN sticker around the lid.
SGOT 1 x SST 2mL
SGPT 1 x SST 2mL
SHBG 1 x SST 2mL
Specimen Collection Manual Issue 19 Page 82 of 95
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Vol
SILVER Yes 1 x EDTA - dedicated tube required
Referred to: Royal North Shore Hospital
2mL
SINDBIS SEROLOGY 1 x SST Referred to: Virology, Queensland Health Forensic and Scientific Services
2mL
SIROLIMUS 1 x EDTA - dedicated tube required
Referred to: PathWest QEII 0.5mL
SJOGREN'S SYNDROME SCREEN 1 x SST 1mL
SKELETAL/STRIATED MUSCLE AB 1 x SST Referred to: PathWest QEII 1mL
SKIN ANTIBODIES 1 x SST Referred to: PathWest QEII 1mL
SKIN PRICK TESTING Test only done by Clinical Immunologist / Allergist. Advise patient to get referral from their GP for a Clinical Immunologist / Allergist.
SKIN SCRAPINGS FOR FUNGAL CULTURE
or Petri Dish DO NOT REFRIGERATE See: WI-COLL-70 - Fungal Sample Collection from Skin Nail Hair
SLA ANTIBODIES 1 x SST Referred to: PathWest QEII 1mL
SLE SCREEN 1 x SST Dependent on history, could also be Lupus Anticoagulant - see Haematology. Call Immunology on 08 9317 0920 if unsure
1mL
SMOOTH MUSCLE ANTIBODY 1 x SST 1mL
SODIUM 1 x SST 2mL
SODIUM (24 HOUR URINE) 1 x Urine Container- 24hr plain
Does not need to be acidified. 2mL
SODIUM (FLUID) 1 x Sterile Container
DOCTOR COLLECT 2mL
SODIUM (SPOT URINE) 1 x Urine Container - random
2mL
SOLUBLE TRANSFERRIN RECEPTOR Yes 1 x SST Referred to: PathWest FSH 1mL
SOLVS (SELF OBTAINED LOW VAGINAL SWAB) MC&S
PATIENT COLLECT SOLVS is a patient collect. Provide patient with Blue Top Gel swab. DO NOT REFRIGERATE
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Vol
SPECIFIC IGE (RAST) Yes 1 x SST Most requests will be fully Medicare rebateable but there will be out-of-pocket for non-specialist requests for more than 4 tests. See FRM-COLL-104 for out-of-pocket fees and test names. Contact Immunology Dept. on 08 9317 0920 for any pricing queries.
2mL
SPINAL MUSCULAR ATROPHY Yes 1 x EDTA - dedicated tube required
Referred to: Genomic Diagnostics. This test is also part of the "Genetic Carrier Screen"
1mL
SPINOCEREBELLAR ATAXIA 2 x EDTA 6mL - Pink, dedicated tube
Referred to: PathWest QEII 2mL
SPIROCHETES 1 x Dry Swab - Orange/White
Referred to: PathWest
SPUTUM CYTOLOGY Urine Container Provide patient with a copy of FRM-COLL-32 Collect deep cough early morning specimen. Do not add anything to the specimen. Where sputum cytology x3 is requested, it is recommended that x3 (three) samples be collected on consecutive days. Where possible, ensure each specimen is sent to the Myaree lab on the day it was collected. If any delays are likely, make sure the specimen is kept refrigerated.
SPUTUM MC&S Urine Container Keep at Room Temperature. See: WI- COLL-71 - Sputum Collection for MC&S
STAT RSV 1 x Dry Swab - Orange/White or 1 x PNA
See WI-COLL-115
STEROID PROFILE 1 x Urine Container- 24hr plain
Referred to: Dorevitch Pathology For children where it may be difficult to collect a full 24-hour specimen, then it may be acceptable to collect the earliest waking urine and then collect for as long as possible. 6-12 hours is acceptable, noting the times urine was collected.
50mL
STEROID SULPHATE ASSAY 1 x EDTA - dedicated tube required
Referred to: Women And Children’s Hospital Adelaide
1mL
STONE ANALYSIS 1 x Urine Container
Referred to: QML pathology
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Vol
STREPTOCOCCAL SEROLOGY 1 x SST Referred to: QML pathology 1mL
STRONGYLOIDES SEROLOGY 1 x SST 2mL
SYNACTHEN STIMULATION TEST 3 x SST This service is offered at Joondalup Hospital on Wednesday morning - by appointment only. Patients are required to make a booking with Dr Steven Ward's rooms on 08 6142 0970. If ACTH co-requested. Collected 2 x chilled EDTA's on the first bleed. See: WI-COLL-64 - Synacthen Stimulation Test
2mL
SYNOVIAL FLUID MC&S DOCTOR COLLECT DO NOT REFRIGERATE
SYNTHETIC CANNABINOIDS Yes Full Chain of Custody Collect If a lab-based test is requested as well, this does not need to be a separate collection. See: WI-COLL-53 - Drugs of Abuse Collection Manual
2x 5mL
SYPHILIS CONFIRMATION 1 x SST Only applicable where Syphilis serology is positive.
SYPHILIS SEROLOGY 1 x SST 2 mL
SYPHILLIS PCR 1 x Dry Swab - Orange/White
Referred to: PathWest QEII Must be a site swab from a lesion.
SYSTEMIC FUNGAL CULTURE (TISSUE MC&S)
T/B/NK 1 x ACD and 1 x EDTA
Full tube of ACD (can use 1mL of EDTA ONLY if paediatric or elderly/infirm). See: WI-COLL-96 - Flow Cytometry
6mL ACD
T3 1 x SST Patient to complete FRM-COLL-45 Patient questionnaire for Thyroid Function Tests
2mL
T4 1 x SST Patient to complete FRM-COLL-45 Patient questionnaire for Thyroid Function Tests
2mL
TACROLIMUS 1 x EDTA - dedicated tube required
Referred to: PathWest QEII 0.5mL
T CELL/TCR GENE RE-ARRANGEMENT STUDIES
Yes 1 x EDTA 6mL - Pink, dedicated tube
Referred to: QML Pathology Other sample types (Doctor collect): 1mL Bone Marrow or sample from Lymph Node or tumor
6mL
TEGRATOL 1 x SST Record date and time of last dose of medication on the form.
2mL
TELLURIUM 1 x SST Referred to: PathWest QEII 1mL
Specimen Collection Manual Issue 19 Page 85 of 95
Test Name Out of
Pocket Specimen Collection Method Min Vol
TERBUTALINE Yes 1 x Urine Container
Referred to: QML Pathology Full Chain of Custody Collect
20mL
TESTICULAR TUMOUR MARKERS 1 x SST 2mL
TESTOSTERONE 1 x SST 2mL
TETANUS SEROLOGY 1 x SST Referred to: QML Pathology 2mL
TFT 1 x SST Patient will only receive FT4 and FT3 results if clinical history that meets Medicare guidelines is documented on the request form, or TSH is abnormal. Patient to complete FRM-COLL-45 Patient questionnaire for Thyroid Function Tests.
2mL
THALLIUM 1 x EDTA - dedicated tube required
Referred to: Dorevitch Pathology 2mL
THALLIUM URINE 1 x Urine Container
Referred to: Laverty Pathology 10mL
THEOPHYLLINE 1 x Lithium Heparin - DO NOT SPIN
Referred to: PathWest QEII Take 4-6 hours post dose. Place DO NOT SPIN sticker around the lid.
0.3mL
THROAT SWAB MC&S Swab - Blue Top Gel
DO NOT REFRIGERATE See: WI-COLL-72 Throat Swab Collection for MC&S
THROMBIN ACTIVATABLE FIBRINOLYSIS INHIBITOR
2 x Sodium Citrate Must be at the lab within 4 hours, Transport in Blue bag or double spin, separate, freeze as per WI-COLL-89. Transport frozen if already frozen. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
THROMBIN ANTI-THROMBIN COMPLEX (TAT)
2 x Sodium Citrate Referred to: PathWest FSH Must be at the lab within 4 hours - transport in blue bag. Or DOUBLE spin/separate/freeze and transport frozen. Patient to complete questionnaire: FRM-COLL-43. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
THROMBIN CLOTTING TIME (TCT) 2 x Sodium Citrate Must be at the lab within 10 hrs or single spin/separate/freeze. Patient to complete questionnaire: FRM-COLL- 43 See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
THROMBIN GENERATION See CALIBRATED AUTOMATED THROMBOGRAM entry for Collection requirements. See: FRM-COLL-86 - Coagulation Collection Guide
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Vol
THROMBOPHILIA SCREEN Yes Collect all the following • 4 x Sodium
Citrate • 2 x EDTA -
dedicated tube required
• 1 x PPT spin within 30 minutes of collection
• 1 x SST
This test has an out of pocket expense unless a pensioner or DVA or relevant medical history. To Myaree within 10hrs -transport in blue bag. Or citrates DOUBLE spun/separated/frozen. Patient to complete questionnaire: FRM-COLL- 43. Thrombophilia screen is made up of the following tests: AT3, PRC, PRS, APCR, Lupus anticoagulant, PGM, FVL, ACL, B2GP1 and Homocysteine.
See: FRM-COLL-86 - Coagulation Collection Guide
THYROGLOBULIN 1 x SST 4mL
THYROID RECEPTOR ANTIBODIES 1 x SST 2mL
THYROID STIMULATING IMMUNOGLOGULINS
1 x SST Referred to: QML Pathology 1mL
TIBC 1 x SST 2mL
TISSUE AUTOANTIBODY SCREEN 1 x SST 1mL
TISSUE FACTOR PATHWAY INHIBITOR
2 x Sodium Citrate Referred to: PathWest FSH Must be at Myaree within 10hrs - transport in Blue bag. Otherwise single spin/separate/freeze. Patient to complete: FRM-COLL-43. See: FRM- COLL-86 - Coagulation Collection Guide
2.7mL
TISSUE PLASMINOGEN ACTIVATOR 2 x Sodium Citrate Referred to: PathWest FSH Must be at Myaree within 10hrs - transport in Blue bag. Otherwise single spin/separate/freeze. Patient to complete: FRM-COLL-43. See: FRM- COLL-86 - Coagulation Collection Guide
2.7mL
TISSUE TRANSGLUTAMINASE ABS (TTG)
1 x SST 1mL
TITANIUM Yes 1 x Trace Element Free - DO NOT SPIN
Referred to: Royal North Shore Hospital (NSW) Place DO NOT SPIN sticker around the lid.
5mL
TOBRAMYCIN 1 x Plain Tube (Red Top) - Spin & Separate
Referred to: PathWest QEII 0.3mL
TOFRANIL 1 x Plain Tube (Red Top)
Referred to: QML Pathology Collect just prior to the next dose or at least 6 hours after the last dose. Provide clinical and medication detail, including time and date of last dose.
2mL
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Vol
TOPOISOMERASE ANTIBODIES 1 x SST 1mL
TOPOMAX 1 x Plain Tube (Red Top)
Referred to: Royal Prince Alfred Hospital
5mL
TORCH SEROLOGY 1 x SST 2mL
TOXOCARA AB 1 x SST Referred to: PathWest QEII 1mL
TOXOPLASMA SEROLOGY 1 x SST 2mL
TPMT 2 x EDTA - dedicated tube required
Referred to: PathWest QEII 3mL each
TPO 1 x SST 1mL
TRANSFERRIN 1 x SST 2mL
TRANSFUSION REACTION INVESTIGATION
2 x EDTA 6mL – Pink and 1 x SST
1. Full Name, DOB or URN, DOC & TOC must be on EDTA tube and request form. All information must match between form and sample.
2. Collector must sign the tube and certifier on form - these signatures must match.
3. In addition to samples offending units and giving set is also required.
6mL
TREPONEMA
TRICHMONAS VAGINALIS PCR 1 x Swab - Cobas or 1 x Urine - Cobas tube or 1 x Dry Swab - Orange/White
See WI-COLL-115 and WI-COLL-81
TRIGLYCERIDE 1 x SST See: WI-COLL-61 - Lipid Testing 2mL
TRIMETHYLAMINE 1 x Urine Container - dedicated jar required. Freeze ASAP
Referred to: Women and Children’s Hospital Adelaide Early morning urine preferred following egg or fish meal the night before.
10mL
TRIPLE TEST 1 x SST - Spin and freeze ASAP
Acceptable gestational age for Triple Test: 14 weeks - 20 weeks. Please fill out Antenatal Patient Questionnaire (FRM-COLL-6).
4mL
TROPONIN (URGENT – Result within 2hours)
1 x SST Must be in the testing lab within 1 hour – call Laboratory Liaising Officer on 08 9317 0814 for Myaree. For country and NT laboratories: call the testing laboratory directly. Collectors to obtain an after-hours contact number for requesting doctors.
2mL
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Vol
TRYPSINOGEN Test Not Available
TSH 1 x SST Patient to complete FRM-COLL-45 Patient questionnaire for Thyroid Function Tests
2mL
TURNER SYNDROME 1 x Lithium Heparin
1mL
TYPHOID CULTURE Faeces Container and/or Blood Culture Bottles
Faeces samples x 3 (these can be on the same day or over several days but preferably successive movements (i.e. one after the another) Provide patient FRM-COLL-13 and explain the collection instructions per the sheet. Blood Culture - see Blood Culture collection WI-COLL-74 See: WI-COLL-73 - Typhoid Culture Samples
TYPHOID PCR Test Not Available
TYPHOID SEROLOGY 1 x SST Referred to: QML Pathology 1mL
TYROSINE 1 x Lithium Heparin - Spin, Separate & Freeze ASAP
Referred to: PathWest QEII 1mL
UA 1 x SST 2mL
ULTRA SENSITIVE PSA Yes 1 x SST Medicare will pay a rebate for only one (1) PSA test performed in any 12- month period in the absence of previously abnormal results or previously diagnosed prostatic disease. A non-refundable fee will be charged if not billed through Medicare. Request form to be stamped with "Patient Advised of Fee" and signed by the patient acknowledging that an out of pocket payment may be due. See: Patient to complete FRM-COLL-36 - Prostate Specific Antigen (PSA) Patient Questionnaire
2mL
URANIUM 1 x Urine Container
Referred to: WorkCover NSW (TestSafe)
10mL
URATE (FLUID) DOCTOR COLLECT 2mL
URATE (SERUM) 1 x SST 2mL
URATE (SPOT URINE) 1 x Urine Container - random
Transfer into Vacutainer (no additive) urine tube. Urine tubes must not be filled beyond half way to prevent spillage.
2mL
Specimen Collection Manual Issue 19 Page 89 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
URATE CLEARANCE 1 x Urine Container- 24hr plain
2mL
UREA 1 x SST 2mL
UREA (24 HOUR URINE) 1 x Urine Container- 24hr plain
2mL
UREA (FLUID) DOCTOR COLLECT 2mL
UREA (SPOT URINE) 1 x Urine Container - random
Transfer into Vacutainer (no additive) urine tube. Urine tubes must not be filled beyond half way to prevent spillage.
2mL
UREA AND CREATININE 1 x SST 2mL
UREA AND ELECTROLYTES 1 x SST Serum must be spun within 4 hours of collection. Sample must not be transported on an ice brick.
2mL
URETHRAL SWAB MC&S DOCTOR COLLECT DO NOT REFRIGERATE
URINARY ALDOSTERONE Yes 1 x Urine Container- 24hr plain
Referred to: Royal Prince Alfred Hospital Samples once aliquoted must be frozen in the lab.
20mL
URINARY HAEMOSIDERIN 1 x Urine Container - random
Urine container to have name, DOB, TOC, DOC all written on the label.
20 mL
URINE CYTOLOGY Urine Container Collect the FIRST part, or all (not mid stream), of the SECOND or later VOID of the day. A spot urine is sufficient if the patient can produce a sample immediately. Keep the specimen refrigerated. Provide patient with a copy of FRM-COLL-27. Where urine cytology x3 is requested, it is recommended that x3 (three) samples be collected on consecutive days. Where possible, ensure each specimen is sent to the Myaree lab on the day it was collected. If any delays are likely, make sure the specimen is kept refrigerated.
10mL
URINE FOR FASTIDIOUS ORGANISMS
Urine Container See WI-COLL-82 Mid-Stream Urine Collection
5mL
URINE HEAVY METAL SCREEN 1 x Urine Container - random
Referred to: Laverty Pathology 10mL
URINE IMMUNOFIXATION 1 x Urine Container - random
If 24-hour urine is specifically requested, then collect 24-hour plain urine.
7mL
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Test Name Out of Pocket Specimen Collection Method Min
Vol
URINE IRON 1 x Urine Container
Referred to: Royal Prince Alfred Hospital
20mL
URINE MC&S Urine Container See WI-COLL-82 Mid-Stream Urine Collection For Stamey’s test (prostatic massage collection): 3x samples will be submitted by patient or doctor. All samples to go under same laboratory number.
5mL
URINE MICROSCOPY Urine Container See WI-COLL-82 Mid-Stream Urine Collection
5mL
URINE OSMOLALITY 1 x Urine Container - random
Serum and urine osmolality must be collected on the same day if both tests are requested. Joondalup, Hollywood and Peel Health Campus also perform Osmolality testing.
2mL
URINE PROTEIN ELECTROPHORESIS
1 x Urine Container - random
If 24-hour urine is specifically requested, then collect 24-hour plain urine.
7mL
URINE RED CELL MORPHOLOGY (URGENT – Result within 2hours)
Urine Container Transport to the laboratory within 1 hour of collection. These samples must be examined immediately upon receipt in the laboratory. See WI-COLL-82 Mid-Stream Urine Collection
10mL
VALPROATE 1 x SST Record date and time of last dose of medication on the form.
2mL
VANADIUM 1 x Urine Container
Referred to: PathWest QEII 10mL
VANCOMYCIN (URGENT – Result before next dosing)
1 x SST Testing performed at Hollywood, Joondalup & Peel Health Campus Labs. NOTE ON REQUEST FORM: (1) time, date and amount of last dose. Less than 1hr before next dose or >11 hours post dose. (2) Injection route: IV; IM; or Oral.
0.5mL
VANCOMYCIN RESISTANT ENTEROCOCCI (VRE SCREEN)
Yes PATIENT COLLECT or Faeces Container
Separate rectal swabs required for VRE screen if requested with CRE screen (ie 2 separate rectal swabs required.) Rectal swab will be a Patient Collect - Provide patient with Blue Top Gel swab. DO NOT REFRIGERATE swabs
VARICELLA ZOSTER PCR 1 x Dry Swab - Orange/White or 1 x Swab - Viral Transport Media
See WI-COLL-115 and FRM-COLL-81 (Swab Collection guide). Please note swabs collected from genital sites are required to be collected by referring doctor.
Specimen Collection Manual Issue 19 Page 91 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
VARICELLA ZOSTER SEROLOGY 1 x SST 2mL
VASCULITIS SCREEN 1 x SST 2mL
VASOACTIVE INTESTINAL PEPTIDE (VIP)
Special Collection Kit
Must be spun and frozen within 1 hour of collection. Call Manual Chemistry for a special tube 08 9317 0838. Sent to RPA.
15mL
VAULT SMEAR DOCTOR COLLECT Refer to PAP SMEAR CYTOLOGY
VENOUS BLOOD GAS DOCTOR COLLECT Test is performed at the hospital sites and must be analysed immediately. Please refer the patient to the nearest hospital.
0.5mL
VERY LONG CHAIN FATTY ACIDS Yes 1 x Lithium Heparin spin, separate & freeze ASAP
Referred to: Women and Children’s Hospital Adelaide or Children’s Hospital Westmead.
2mL
VIRAL SEROLOGY AND ATYPICAL SEROLOGY
1 x SST 2mL
VISCOSITY 3 x EDTA - dedicated tube required
Referred to: PathWest QEII Must be at RPH within 4 hours. If delayed spin and separate samples. Must be at RPH before 2pm day of collection. Only Collect Monday to Thursday. DO NOT Collect on Public Holidays.
3mL
VITAMIN A 1 x SST - spin freeze and foil
Referred to: QML pathology 3mL
VITAMIN B1 1 x EDTA - freeze whole and foil
Referred to: QML Pathology 4mL
VITAMIN B2 1 x EDTA - freeze whole and foil
Referred to: QML Pathology 2mL
VITAMIN B3 1 x Urine Container- 24hr acid
Referred to: Royal Prince Alfred Hospital
10mL
VITAMIN B6 1 x EDTA - freeze whole and foil
Referred to: QML Pathology 2mL
VITAMIN B7 Test Not Available
VITAMIN C 1 x SST spin separate and freeze ASAP
Referred to: QML Pathology Patient must be fasting.
2mL
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Test Name Out of Pocket Specimen Collection Method Min
Vol
VITAMIN D Yes 1 x SST Western Diagnostic Pathology will bulk bill when indicated by referring doctor. WDP assume requesting doctor has determined that patient meets requirements as stipulated in the Medicare Benefits Schedule (Item No: 66833) Request form to be stamped with "Patient Advised of Fee" and signed by the patient acknowledging that an out of pocket payment will be due. See: WI-COLL- 113 Vitamin D Bulk Billing Criteria
2mL
VITAMIN E 1 x SST - spin freeze and foil
Referred to: QML Pathology 3mL
VITAMIN K 1 x Lithium Heparin - Spin, Separate, Foil & Freeze ASAP
Referred to: SEALS (Prince of Wales, NSW)
2mL
VON WILLEBRAND SCREEN See FACTOR STUDIES entry for Collection requirements. See: FRM- COLL-86 - Coagulation Collection Guide
VORICONAZOLE 1 x Plain Tube (Red Top)
Referred to: Royal Brisbane Hospital (Path Queensland)
1mL
WEST NILE VIRUS 1 x SST This test is referred to and performed by PathWest QEII.
2mL
WHIPPLE'S DISEASE 1 x EDTA - dedicated tube required
Referred to: PathWest QEII 2mL
WHITE CELL COUNT 1 x EDTA Full Name, DOB or URN, DOC & TOC must be on EDTA tube. Collector must sign EDTA tube and request form. Transport chilled. NOTE: Only 1 EDTA required for any combination of FBC/Indices, ESR, Malaria, RET, HbA1C, HE.
1ml
WHITE CELL COUNT & DIFFERENTIAL (PATIENTS ON CLOZAPINE ONLY)
1 x EDTA Sample must be labelled with full name, DOB or URN, DOC &TOC. Tube & request form certifier must be signed by collector. Transport chilled. Patient's on Clozapine therapy.
1ml
WHITE CELL ENZYMES Yes 1 x EDTA - dedicated tube required
Referred to: Women and Children’s Hospital Adelaide CONSENT REQUIRED DUE TO LARGE OUT OF POCKET EXPENSE
1mL
WILLIAMS SYNDROME See MICROARRAY - BLOOD
Specimen Collection Manual Issue 19 Page 93 of 95
Test Name Out of Pocket Specimen Collection Method Min
Vol
WILSON’S DISEASE 1 x Urine Container- 24hr plain
Referred to: Laverty Pathology Only test for genetics when specifically requested. Otherwise a 24hr urine for 'Copper' is collected.
10mL
WILSON’S DISEASE GENE 4 x EDTA 6mL - Pink, dedicated tube
Referred to: PathWest QEII 6mL
WOUND SWAB MC&S Swab - Blue Top Gel
See Wound swab WI-COLL-81 DO NOT REFRIGERATE
XACT See FACTOR X- ACTIVATED CLOTTING TIME entry for Collection requirements. See: FRM- COLL-86 - Coagulation Collection Guide
XYMOPHEN OR XYMOGEN 2 x Sodium Citrate Referred to: PathWest FSH To Myaree within 4hrs, transport in blue bag. Or, DOUBLE spin, separate and freeze and transport frozen. See: FRM-COLL-86 - Coagulation Collection Guide
2.7mL
Y CHROMOSOME DELETION 1 x EDTA 6mL - Pink, dedicated tube
Referred to: QML Pathology 6mL
YELLOW FEVER 1 x SST Referred to: PathWest QEII 2mL
YERSINIA SEROLOGY 1 x SST Referred to: QML Pathology 2mL
ZIKA VIRUS PCR 1 x EDTA - dedicated tube required and 1 x Urine Container - random
Referred to: PathWest QEII Record details of the country visited. How long the patient stayed in the country. If pregnant or trying.
1mL blood 10mL urine
ZIKA VIRUS SEROLOGY 1 x SST Referred to: PathWest QEII Record details of the country visited. How long the patient stayed in the country. If pregnant or trying.
2mL
ZINC PROTOPORPHYRIN Yes 1 x EDTA 6mL - Pink, dedicated tube
Referred to: QML Pathology 3mL
ZINC SERUM 1 x Trace Element Free - spin & separate
Referred to: Laverty Pathology 2mL
ZINC URINE 1 x Urine Container - random
Referred to: Laverty Pathology 20mL
ZIPRASIDONE Test Not Available
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Test Name Out of Pocket Specimen Collection Method Min
Vol
ZNT8 ABS 1 x SST Referred to: Sullivan Nicolaides 2mL
ZYMUPHEN MICROPARTICLE ACTIVITY
See PLATELET MICROPARTICLE entry for collection requirements. See: FRM-COLL-86 - Coagulation Collection Guide
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