http://oilab.seas.wustl.edu -- 1

In Vivo Optical Imaging: Human Application?

In Vivo Optical Imaging: Human Application?

Vassiliy Tsytsarev

University of Maryland school of Medicine

E-mail: tsytsarev@umaryland.edu

http://oilab.seas.wustl.edu -- 2

Glucose MetabolismGlucose Metabolism

Capillary

Glycogen

Yongmei Chen and Raymond A SwansonJournal of Cerebral Blood Flow & Metabolism (2003) 23, 137–149; Astrocytes and Brain Injury

TCA

TCA

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Glucose Fluorescence Substitute: 2-NBDGGlucose Fluorescence Substitute: 2-NBDG

2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-d-glucopyranose (2-NBDG)

2-deoxy-d-glucose

D-glucose

http://oilab.seas.wustl.edu -- 4

Glucose Fluorescence Substitute:In vivo imaging of epileptic activity using 2-

NBDG

Glucose Fluorescence Substitute:In vivo imaging of epileptic activity using 2-

NBDG

http://oilab.seas.wustl.edu -- 5

Epileptic Seizures: Fluorescence ImagingEpileptic Seizures: Fluorescence Imaging

http://oilab.seas.wustl.edu -- 6

fMRI vs NIRSfMRI vs NIRS

http://oilab.seas.wustl.edu -- 7

Light BananaLight Banana

http://oilab.seas.wustl.edu -- 8

Light Banana: OxygenationLight Banana: Oxygenation

http://oilab.seas.wustl.edu -- 9

Main parts of experimental setupMain parts of experimental setup

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HITACHI: medical applicationHITACHI: medical application

http://oilab.seas.wustl.edu -- 11

Spectrum: T-raySpectrum: T-ray

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EROS: Optical correlation of the EEG?EROS: Optical correlation of the EEG?

Evoked Response Optical Signal (EROS) (Gratton et al, 2002, 6, 7)

http://oilab.seas.wustl.edu -- 13

T-ray imagingT-ray imaging

http://oilab.seas.wustl.edu -- 14

AcknowledgmentsAcknowledgments

Drs. Shigeru Tanaka, Hidenao Fukuyama, Kazuyuki Imamura, Ayako Ajima, Hisayuki Ojima, Minoru Kimura and Jerom Ribot – Brain Science Institute of RIKEN and Kyoto University, Japan

Dr. Sonya Bahar, Director, Center for Neurodynamics, University of Missouri at St. Louis

Daisuke Takeshita and Douglas Joseph Brumm – Ph.D students, Center for Neurodynamics, University of Missouri at St. Louis

Song Hu, Junjie Yao, Li Li – Ph.D. student of the Washington University in St. Louis

Drs Konstantin Maslov and Lihong Wang – Department of Biomedical Engineering, Washington University in St. Louis

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Thank you very much for your attention Thank you very much for your attention