Embed Size (px)
Citation preview
Console Software
The ACQUITY UPLC PDA Detector is equipped with a customizable instrument console, for both Empower™ and MassLynx™ Software, that enables users to easily stay in control of their detector. Instrument setup, status monitoring, and diagnostics are easily accessible through an intuitive, easy-to-learn interface.
The console uses a simple navigational approach, combined with instrument default parameters, defined range settings, tool tips, and online help, that makes system navigation and instrument usability a snap. Furthermore, at any time the control panel can display the operating status of the module providing assurance that the system is working properly.
ACQUITY UPLC PhoTodIode ArrAY deTeCTor
The Waters® ACQUITY UPLC™ PDA Detector is specifically designed to complement the core ACQUITY UPLC system. With data rates up to 80 Hz and noise specifications as low as 10 µAU, the ACQUITY UPLC PDA Detector offers sensitivity that is unmatched by any other photodiode array on the market. Further, enhanced software control provides flexibility for simultaneous 2D and 3D operation in either Empower™ or MassLynx™ Software.
PDA detector features:
• Unequaled trace impurity detection and quantification using maximum chromatographic sensitivity
• Independent optimization of data rate and filtering constants
• ACQUITY UPLC light-guiding flow cells
• Superior linear range with constant optical bandpass
• Console software
Worldwide services and support
• Analytical Instrumentation and Software Services include Total Assurance Plans and Warranties that extend and enhance the original warranty you receive when you buy a Waters product. These plans minimize the level of insurance investment and deliver the value you need to avoid costly and time-consuming system downtime.
• Connections Compliance Services provide you with timely and cost-efficient solutions for your regulatory compliance challenges. You can use Waters Compliance Services to verify proper equipment operation for CGMP/GLP compliance, significantly reducing operating costs.
• Educational Services provide extensive HPLC and MS training and education at your site, at our corporate headquarters or at our local offices around the world.
• Representatives of Waters Global Customer Assurance Organization, trained and certified in all Waters products and current in LC and MS applications, are available in person, on the phone, via FAX or at www.waters.com to answer questions and provide you with service, support and information.
Waters, ACQUITY UPLC, UPLC, Ultra Performance LC, Empower, and MassLynx are trademarks of Waters Corporation.Teflon is a registered trademark of E.I. du Port de Nemours and Company. All other trademarks are the property of their respective owners.©2006 Waters Corporation Printed in the U.S.A. March 2006 720001332EN MC-CV
Sales offices:Austria 43 1 877 18 07
Australia 61 2 9933 1777
Belgium and Luxembourg 32 2 726 1000
Brazil 55 11 5094 3788
Canada 1 800 252 4752
China 86 10 8451 8918
Czech republic 420 2 617 11384
denmark 45 46 59 8080
Finland 358 9 506 4140
France 33 1 3048 7200
Germany 49 6196 400 7000
hong Kong 852 29 64 1800
hungary 36 1 350 5086
India 91 80 2837 1900
Ireland 353 1 448 1500
Italy 39 02 27 4211
Japan 81 3 3471 7191
Korea 82 2 820 2700
Mexico 52 55 5200 1860
The Netherlands 31 76 508 7200
Norway 47 6 384 6050
Poland 48 22 833 4400
Puerto rico 787 747 8445
russia/CIS 7 495 3367000
Singapore 65 6273 1221
Spain 34 93 600 9300
Sweden 46 8 555 11 500
Switzerland 41 62 889 2030
Taiwan 886 2 2543 1898
UK 44 208 238 6100
US 1 800 252 4752
WATERS CORPORATION 34 Maple St. Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com
Waters ACQUITY UPLC System with the PDA Detector.
optimized for UPLC separations
Waters ACQUITY UPLC Systems have been shown to significantly reduce run times. The ACQUITY UPLC PDA Detector, coupled with innovative ACQUITY UPLC column chemistries and separations modules, leverages ultra-low dwell volumes and low-noise, high-speed detection to bring you productivity, sensitivity, and resolution that are unprecedented in HPLC techniques today.
Additionally, the PDA Detector may be interfaced directly with mass spectrometers. The ACQUITY UPLC System’s low dispersion fluidics eliminate the need to remove the optical detector, or to split the flow path between the two detectors, in order to maintain optimal resolution.
Trace Impurity detection
The Waters ACQUITY UPLC PDA Detector has exceptional signal-to-noise ratios, high optical and digital resolution, and high-sensitivity library matching. This enables the ACQUITY UPLC System to map low levels of compounds and determine trace impurity levels within the compound’s peak. Unique Lamp Optimization Software optimizes light intensity and maintains minimal noise level across the entire deuterium spectrum over the lifetime of the lamp. The need to compromise optical bandpass and linear range simply to reduce noise is eliminated.
Superior linear range
The ACQUITY UPLC PDA detector can acquire chromatographic and spectral data at wavelengths 190 to 500 nm and up to 2.0 AU, without compromising linearity. Such conformity is crucial when comparing spectra across a broad wavelength and concentration range. Without it, disparate levels in common stability indicating methods cannot be quantified at the wavelength that offers the best signal-to-noise and minimal interference.
Independent optimization of data rate and Filtering Constants
UPLC Systems can generate peak widths as low as one second half-height, a detection challenge for which the ACQUITY UPLC PDA Detector is specifically designed. The detector has a high sampling rate to perform accurate and reproducible integration of the analyte peak, while allowing for independent optimization of the data rate and the filtering constants for the best sensitivity and resolution.
Light rays entering the liquid core of the flow cell are internally reflected when they meet the Teflon AF boundary. These rays are transmitted through the cell without loss, except for absorption by the sample.
Light-guiding flow cell technology
Small bore high-capacity ACQUITY UPLC Columns produce small volume peaks. To avoid band spreading and maintain concentration, the detector flow cell volume must be correspondingly low. To achieve the required volume reduction with conventional absorbance detector flow cells, the pathlength must be reduced, which, as predicted by Beer’s law, results in a loss of sensitivity.
Waters has specifically designed a low volume light-guiding flow cell for the ACQUITY UPLC PDA Detector that has optimum path length and high light throughput. The cell uses Teflon® AF, utilizing total internal reflection principles, to improve light transmission efficiency by eliminating internal absorption.
Plotting the detector response versus concentration demonstrates the linear range and linearity for high concentration actives and low concentration impurities.
Optimizing for sensitivity and resolution with different data rates and different filter constants provides the best results. The simultaneous collection of 3D data provides the spectral information needed for library matching and peak purity analysis.
The ACQUITY UPLC PDA detector allows quantitation of impurities at levels down to 0.004%.
650.0815.007Impurity 7
7092799.8454.484Oxybutynin
560.0443.366Impurity 6
100.0082.557Impurity 5
200.0122.439Impurity 4
60.0031.495Impurity 3
110.0041.175Impurity 2
70.0041.005Impurity 1
Signal-to-Noise
Area %Retention Time(Minutes)
Compound
Fast Time Constant Normal Time Constant
10 Hz
20 Hz
40 Hz
80 Hz
Minutes0.100 0.125 0.150 0.175 0.200 0.225 0.250
Minutes0.100 0.125 0.150 0.175 0.200 0.225 0.250
Sample: Beta Blockers Mix
optimized for UPLC separations
Waters ACQUITY UPLC Systems have been shown to significantly reduce run times. The ACQUITY UPLC PDA Detector, coupled with innovative ACQUITY UPLC column chemistries and separations modules, leverages ultra-low dwell volumes and low-noise, high-speed detection to bring you productivity, sensitivity, and resolution that are unprecedented in HPLC techniques today.
Additionally, the PDA Detector may be interfaced directly with mass spectrometers. The ACQUITY UPLC System’s low dispersion fluidics eliminate the need to remove the optical detector, or to split the flow path between the two detectors, in order to maintain optimal resolution.
Trace Impurity detection
The Waters ACQUITY UPLC PDA Detector has exceptional signal-to-noise ratios, high optical and digital resolution, and high-sensitivity library matching. This enables the ACQUITY UPLC System to map low levels of compounds and determine trace impurity levels within the compound’s peak. Unique Lamp Optimization Software optimizes light intensity and maintains minimal noise level across the entire deuterium spectrum over the lifetime of the lamp. The need to compromise optical bandpass and linear range simply to reduce noise is eliminated.
Superior linear range
The ACQUITY UPLC PDA detector can acquire chromatographic and spectral data at wavelengths 190 to 500 nm and up to 2.0 AU, without compromising linearity. Such conformity is crucial when comparing spectra across a broad wavelength and concentration range. Without it, disparate levels in common stability indicating methods cannot be quantified at the wavelength that offers the best signal-to-noise and minimal interference.
Independent optimization of data rate and Filtering Constants
UPLC Systems can generate peak widths as low as one second half-height, a detection challenge for which the ACQUITY UPLC PDA Detector is specifically designed. The detector has a high sampling rate to perform accurate and reproducible integration of the analyte peak, while allowing for independent optimization of the data rate and the filtering constants for the best sensitivity and resolution.
Light rays entering the liquid core of the flow cell are internally reflected when they meet the Teflon AF boundary. These rays are transmitted through the cell without loss, except for absorption by the sample.
Light-guiding flow cell technology
Small bore high-capacity ACQUITY UPLC Columns produce small volume peaks. To avoid band spreading and maintain concentration, the detector flow cell volume must be correspondingly low. To achieve the required volume reduction with conventional absorbance detector flow cells, the pathlength must be reduced, which, as predicted by Beer’s law, results in a loss of sensitivity.
Waters has specifically designed a low volume light-guiding flow cell for the ACQUITY UPLC PDA Detector that has optimum path length and high light throughput. The cell uses Teflon® AF, utilizing total internal reflection principles, to improve light transmission efficiency by eliminating internal absorption.
Plotting the detector response versus concentration demonstrates the linear range and linearity for high concentration actives and low concentration impurities.
Optimizing for sensitivity and resolution with different data rates and different filter constants provides the best results. The simultaneous collection of 3D data provides the spectral information needed for library matching and peak purity analysis.
The ACQUITY UPLC PDA detector allows quantitation of impurities at levels down to 0.004%.
650.0815.007Impurity 7
7092799.8454.484Oxybutynin
560.0443.366Impurity 6
100.0082.557Impurity 5
200.0122.439Impurity 4
60.0031.495Impurity 3
110.0041.175Impurity 2
70.0041.005Impurity 1
Signal-to-Noise
Area %Retention Time(Minutes)
Compound
Fast Time Constant Normal Time Constant
10 Hz
20 Hz
40 Hz
80 Hz
Minutes0.100 0.125 0.150 0.175 0.200 0.225 0.250
Minutes0.100 0.125 0.150 0.175 0.200 0.225 0.250
Sample: Beta Blockers Mix
optimized for UPLC separations
Waters ACQUITY UPLC Systems have been shown to significantly reduce run times. The ACQUITY UPLC PDA Detector, coupled with innovative ACQUITY UPLC column chemistries and separations modules, leverages ultra-low dwell volumes and low-noise, high-speed detection to bring you productivity, sensitivity, and resolution that are unprecedented in HPLC techniques today.
Additionally, the PDA Detector may be interfaced directly with mass spectrometers. The ACQUITY UPLC System’s low dispersion fluidics eliminate the need to remove the optical detector, or to split the flow path between the two detectors, in order to maintain optimal resolution.
Trace Impurity detection
The Waters ACQUITY UPLC PDA Detector has exceptional signal-to-noise ratios, high optical and digital resolution, and high-sensitivity library matching. This enables the ACQUITY UPLC System to map low levels of compounds and determine trace impurity levels within the compound’s peak. Unique Lamp Optimization Software optimizes light intensity and maintains minimal noise level across the entire deuterium spectrum over the lifetime of the lamp. The need to compromise optical bandpass and linear range simply to reduce noise is eliminated.
Superior linear range
The ACQUITY UPLC PDA detector can acquire chromatographic and spectral data at wavelengths 190 to 500 nm and up to 2.0 AU, without compromising linearity. Such conformity is crucial when comparing spectra across a broad wavelength and concentration range. Without it, disparate levels in common stability indicating methods cannot be quantified at the wavelength that offers the best signal-to-noise and minimal interference.
Independent optimization of data rate and Filtering Constants
UPLC Systems can generate peak widths as low as one second half-height, a detection challenge for which the ACQUITY UPLC PDA Detector is specifically designed. The detector has a high sampling rate to perform accurate and reproducible integration of the analyte peak, while allowing for independent optimization of the data rate and the filtering constants for the best sensitivity and resolution.
Light rays entering the liquid core of the flow cell are internally reflected when they meet the Teflon AF boundary. These rays are transmitted through the cell without loss, except for absorption by the sample.
Light-guiding flow cell technology
Small bore high-capacity ACQUITY UPLC Columns produce small volume peaks. To avoid band spreading and maintain concentration, the detector flow cell volume must be correspondingly low. To achieve the required volume reduction with conventional absorbance detector flow cells, the pathlength must be reduced, which, as predicted by Beer’s law, results in a loss of sensitivity.
Waters has specifically designed a low volume light-guiding flow cell for the ACQUITY UPLC PDA Detector that has optimum path length and high light throughput. The cell uses Teflon® AF, utilizing total internal reflection principles, to improve light transmission efficiency by eliminating internal absorption.
Plotting the detector response versus concentration demonstrates the linear range and linearity for high concentration actives and low concentration impurities.
Optimizing for sensitivity and resolution with different data rates and different filter constants provides the best results. The simultaneous collection of 3D data provides the spectral information needed for library matching and peak purity analysis.
The ACQUITY UPLC PDA detector allows quantitation of impurities at levels down to 0.004%.
650.0815.007Impurity 7
7092799.8454.484Oxybutynin
560.0443.366Impurity 6
100.0082.557Impurity 5
200.0122.439Impurity 4
60.0031.495Impurity 3
110.0041.175Impurity 2
70.0041.005Impurity 1
Signal-to-Noise
Area %Retention Time(Minutes)
Compound
Fast Time Constant Normal Time Constant
10 Hz
20 Hz
40 Hz
80 Hz
Minutes0.100 0.125 0.150 0.175 0.200 0.225 0.250
Minutes0.100 0.125 0.150 0.175 0.200 0.225 0.250
Sample: Beta Blockers Mix
Console Software
The ACQUITY UPLC PDA Detector is equipped with a customizable instrument console, for both Empower™ and MassLynx™ Software, that enables users to easily stay in control of their detector. Instrument setup, status monitoring, and diagnostics are easily accessible through an intuitive, easy-to-learn interface.
The console uses a simple navigational approach, combined with instrument default parameters, defined range settings, tool tips, and online help, that makes system navigation and instrument usability a snap. Furthermore, at any time the control panel can display the operating status of the module providing assurance that the system is working properly.
ACQUITY UPLC PhoTodIode ArrAY deTeCTor
The Waters® ACQUITY UPLC™ PDA Detector is specifically designed to complement the core ACQUITY UPLC system. With data rates up to 80 Hz and noise specifications as low as 10 µAU, the ACQUITY UPLC PDA Detector offers sensitivity that is unmatched by any other photodiode array on the market. Further, enhanced software control provides flexibility for simultaneous 2D and 3D operation in either Empower™ or MassLynx™ Software.
PDA detector features:
• Unequaled trace impurity detection and quantification using maximum chromatographic sensitivity
• Independent optimization of data rate and filtering constants
• ACQUITY UPLC light-guiding flow cells
• Superior linear range with constant optical bandpass
• Console software
Worldwide services and support
• Analytical Instrumentation and Software Services include Total Assurance Plans and Warranties that extend and enhance the original warranty you receive when you buy a Waters product. These plans minimize the level of insurance investment and deliver the value you need to avoid costly and time-consuming system downtime.
• Connections Compliance Services provide you with timely and cost-efficient solutions for your regulatory compliance challenges. You can use Waters Compliance Services to verify proper equipment operation for CGMP/GLP compliance, significantly reducing operating costs.
• Educational Services provide extensive HPLC and MS training and education at your site, at our corporate headquarters or at our local offices around the world.
• Representatives of Waters Global Customer Assurance Organization, trained and certified in all Waters products and current in LC and MS applications, are available in person, on the phone, via FAX or at www.waters.com to answer questions and provide you with service, support and information.
Waters, ACQUITY UPLC, UPLC, Ultra Performance LC, Empower, and MassLynx are trademarks of Waters Corporation.Teflon is a registered trademark of E.I. du Port de Nemours and Company. All other trademarks are the property of their respective owners.©2006 Waters Corporation Printed in the U.S.A. March 2006 720001332EN MC-CV
Sales offices:Austria 43 1 877 18 07
Australia 61 2 9933 1777
Belgium and Luxembourg 32 2 726 1000
Brazil 55 11 5094 3788
Canada 1 800 252 4752
China 86 10 8451 8918
Czech republic 420 2 617 11384
denmark 45 46 59 8080
Finland 358 9 506 4140
France 33 1 3048 7200
Germany 49 6196 400 7000
hong Kong 852 29 64 1800
hungary 36 1 350 5086
India 91 80 2837 1900
Ireland 353 1 448 1500
Italy 39 02 27 4211
Japan 81 3 3471 7191
Korea 82 2 820 2700
Mexico 52 55 5200 1860
The Netherlands 31 76 508 7200
Norway 47 6 384 6050
Poland 48 22 833 4400
Puerto rico 787 747 8445
russia/CIS 7 495 3367000
Singapore 65 6273 1221
Spain 34 93 600 9300
Sweden 46 8 555 11 500
Switzerland 41 62 889 2030
Taiwan 886 2 2543 1898
UK 44 208 238 6100
US 1 800 252 4752
WATERS CORPORATION 34 Maple St. Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com
Waters ACQUITY UPLC System with the PDA Detector.
Console Software
The ACQUITY UPLC PDA Detector is equipped with a customizable instrument console, for both Empower™ and MassLynx™ Software, that enables users to easily stay in control of their detector. Instrument setup, status monitoring, and diagnostics are easily accessible through an intuitive, easy-to-learn interface.
The console uses a simple navigational approach, combined with instrument default parameters, defined range settings, tool tips, and online help, that makes system navigation and instrument usability a snap. Furthermore, at any time the control panel can display the operating status of the module providing assurance that the system is working properly.
ACQUITY UPLC PhoTodIode ArrAY deTeCTor
The Waters® ACQUITY UPLC™ PDA Detector is specifically designed to complement the core ACQUITY UPLC system. With data rates up to 80 Hz and noise specifications as low as 10 µAU, the ACQUITY UPLC PDA Detector offers sensitivity that is unmatched by any other photodiode array on the market. Further, enhanced software control provides flexibility for simultaneous 2D and 3D operation in either Empower™ or MassLynx™ Software.
PDA detector features:
• Unequaled trace impurity detection and quantification using maximum chromatographic sensitivity
• Independent optimization of data rate and filtering constants
• ACQUITY UPLC light-guiding flow cells
• Superior linear range with constant optical bandpass
• Console software
Worldwide services and support
• Analytical Instrumentation and Software Services include Total Assurance Plans and Warranties that extend and enhance the original warranty you receive when you buy a Waters product. These plans minimize the level of insurance investment and deliver the value you need to avoid costly and time-consuming system downtime.
• Connections Compliance Services provide you with timely and cost-efficient solutions for your regulatory compliance challenges. You can use Waters Compliance Services to verify proper equipment operation for CGMP/GLP compliance, significantly reducing operating costs.
• Educational Services provide extensive HPLC and MS training and education at your site, at our corporate headquarters or at our local offices around the world.
• Representatives of Waters Global Customer Assurance Organization, trained and certified in all Waters products and current in LC and MS applications, are available in person, on the phone, via FAX or at www.waters.com to answer questions and provide you with service, support and information.
Waters, ACQUITY UPLC, UPLC, Ultra Performance LC, Empower, and MassLynx are trademarks of Waters Corporation.Teflon is a registered trademark of E.I. du Port de Nemours and Company. All other trademarks are the property of their respective owners.©2006 Waters Corporation Printed in the U.S.A. March 2006 720001332EN MC-CV
Sales offices:Austria 43 1 877 18 07
Australia 61 2 9933 1777
Belgium and Luxembourg 32 2 726 1000
Brazil 55 11 5094 3788
Canada 1 800 252 4752
China 86 10 8451 8918
Czech republic 420 2 617 11384
denmark 45 46 59 8080
Finland 358 9 506 4140
France 33 1 3048 7200
Germany 49 6196 400 7000
hong Kong 852 29 64 1800
hungary 36 1 350 5086
India 91 80 2837 1900
Ireland 353 1 448 1500
Italy 39 02 27 4211
Japan 81 3 3471 7191
Korea 82 2 820 2700
Mexico 52 55 5200 1860
The Netherlands 31 76 508 7200
Norway 47 6 384 6050
Poland 48 22 833 4400
Puerto rico 787 747 8445
russia/CIS 7 495 3367000
Singapore 65 6273 1221
Spain 34 93 600 9300
Sweden 46 8 555 11 500
Switzerland 41 62 889 2030
Taiwan 886 2 2543 1898
UK 44 208 238 6100
US 1 800 252 4752
WATERS CORPORATION 34 Maple St. Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com
Waters ACQUITY UPLC System with the PDA Detector.
